In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen

Detalhes bibliográficos
Autor(a) principal: Silva, Daiane Moreira
Data de Publicação: 2019
Outros Autores: Holden, Shauna Ann, Souza, José Camisão de, Fair, Sean
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/40599
Resumo: High seminal quality is important to achieve acceptable pregnancy rate following artificial insemination with cryopreserved semen in the equine industry. Docosahexaenoic acid (DHA) is an omega-3-polyunsaturated acid, which improves the integrity of the spermatozoa membrane during temperature changes. Insulin-like growth factor-I (IGF-I) is a protein hormone that helps mainly glucose to enter spermatozoa and it is an antioxidant. The aim of this study was to assess the effect of the in vitro addition of DHA in combination with IGF-I to frozen-thawed stallion semen. Three ejaculates from each of three Irish Sport Horse stallions were collected, the gel fraction was removed and semen was diluted in a 1:1 ratio using extender, centrifuged (1.000 g) for 10 minutes at 32 °C and ressuspended to 100 x 106 spermatozoa/mL in freezing extender. Semen was cooled to 4 °C, packed into 0.5 mL straws, frozen and stored under liquid nitrogen at -196 °C. Straws were thawed at 37 °C for 30 seconds, semen was diluted to 25 x 106 spermatozoa/mL and split in four treatments adding 0 or 1 ng of DHA /mL and 0 or 100 ng of IGF-I /mL: DHA0, DHA0 + IGF-I, DHA1 and DHA1 + IGF-I. Semen was incubated at 32 °C and after 30 minutes, total motility (TM), rapid progressive motility (PM), viability and acrosome integrity were assessed. After 60 and 120 minutes, TM and PM were assessed again. Post-thawed PM was higher (P < 0.05) when DHA1 + IGF-I was added, but there was no effect of the addition of DHA and IGF-I to TM, viability or acrosome integrity (P > 0.05).
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spelling In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semenAdição in vitro de DHA e IGF-I aumenta a motilidade progressiva do sêmen criopreservado de garanhõesEquine - SpermatozoaOmega-3AntioxidantHormoneEquinos - EspermatozoidesÔmega-3AntioxidanteHormôniosHigh seminal quality is important to achieve acceptable pregnancy rate following artificial insemination with cryopreserved semen in the equine industry. Docosahexaenoic acid (DHA) is an omega-3-polyunsaturated acid, which improves the integrity of the spermatozoa membrane during temperature changes. Insulin-like growth factor-I (IGF-I) is a protein hormone that helps mainly glucose to enter spermatozoa and it is an antioxidant. The aim of this study was to assess the effect of the in vitro addition of DHA in combination with IGF-I to frozen-thawed stallion semen. Three ejaculates from each of three Irish Sport Horse stallions were collected, the gel fraction was removed and semen was diluted in a 1:1 ratio using extender, centrifuged (1.000 g) for 10 minutes at 32 °C and ressuspended to 100 x 106 spermatozoa/mL in freezing extender. Semen was cooled to 4 °C, packed into 0.5 mL straws, frozen and stored under liquid nitrogen at -196 °C. Straws were thawed at 37 °C for 30 seconds, semen was diluted to 25 x 106 spermatozoa/mL and split in four treatments adding 0 or 1 ng of DHA /mL and 0 or 100 ng of IGF-I /mL: DHA0, DHA0 + IGF-I, DHA1 and DHA1 + IGF-I. Semen was incubated at 32 °C and after 30 minutes, total motility (TM), rapid progressive motility (PM), viability and acrosome integrity were assessed. After 60 and 120 minutes, TM and PM were assessed again. Post-thawed PM was higher (P < 0.05) when DHA1 + IGF-I was added, but there was no effect of the addition of DHA and IGF-I to TM, viability or acrosome integrity (P > 0.05).Alta qualidade seminal é importante para alcançar taxa de gestação aceitável por meio da inseminação artificial na equinocultura. Ácido docosahexaenoico (DHA) é um ácido graxo poliinsaturado também conhecido como ômega-3, que melhora a integridade da membrana espermática durante as mudanças de temperatura. Hormônio do crescimento semelhante à insulina I (IGF-I) é um hormônio proteico que auxilia principalmente a glicose a entrar no espermatozoide, além de ser um antioxidante. O objetivo deste estudo foi analisar o efeito da adição in vitro de DHA combinado com IGF-I no sêmen congelado-descongelado de garanhões. Três ejaculados de cada um de três garanhões da raça Irish Sport Horse foram coletados, a fração gel foi removida e o sêmen foi diluído na proporção 1:1 usando diluidor, em seguida, o sêmen foi centrifugado (1000 g) por 10 minutos a 32°C e ressuspenso a 100 x 106 espermatozoides/mL em diluidor de congelamento. O sêmen foi resfriado a 4°C, envasado em palhetas de 0,5 mL, congelado e estocado em nitrogênio líquido a -196°C. As palhetas foram descongeladas a 37°C por 30 segundos, o sêmen foi diluído a 25 x 106 espermatozoides/mL e foi dividido em quatro tratamentos, nos quais se adicionou 0 ou 1 ng de DHA/mL e 0 ou 100 ng de IGF-I /mL: DHA0, DHA0 + IGF-I, DHA1 e DHA1 + IGF-I. O sêmen foi incubado a 32°C e, depois de 30 minutos, a motilidade total (TM), motilidade progressiva rápida (PM), viabilidade e integrade acrossômica foram avaliadas. Depois de 60 e 120 minutos, TM e PM foram analisadas novamente. A PM após o descongelamento foi superior (P 0,05).Instituto Federal de Educação, Ciência e Tecnologia do Sul de Minas Gerais - IFSULDEMINAS2020-05-05T17:58:35Z2020-05-05T17:58:35Z2019-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSILVA, D. M. et al. In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion sêmen. Revista Agrogeoambiental, Pouso Alegre, v. 11, n. 2, p. 127-138, jun. 2019.http://repositorio.ufla.br/jspui/handle/1/40599Revista Agrogeoambientalreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSilva, Daiane MoreiraHolden, Shauna AnnSouza, José Camisão deFair, Seaneng2020-05-05T17:58:56Zoai:localhost:1/40599Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-05-05T17:58:56Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen
Adição in vitro de DHA e IGF-I aumenta a motilidade progressiva do sêmen criopreservado de garanhões
title In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen
spellingShingle In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen
Silva, Daiane Moreira
Equine - Spermatozoa
Omega-3
Antioxidant
Hormone
Equinos - Espermatozoides
Ômega-3
Antioxidante
Hormônios
title_short In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen
title_full In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen
title_fullStr In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen
title_full_unstemmed In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen
title_sort In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion semen
author Silva, Daiane Moreira
author_facet Silva, Daiane Moreira
Holden, Shauna Ann
Souza, José Camisão de
Fair, Sean
author_role author
author2 Holden, Shauna Ann
Souza, José Camisão de
Fair, Sean
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva, Daiane Moreira
Holden, Shauna Ann
Souza, José Camisão de
Fair, Sean
dc.subject.por.fl_str_mv Equine - Spermatozoa
Omega-3
Antioxidant
Hormone
Equinos - Espermatozoides
Ômega-3
Antioxidante
Hormônios
topic Equine - Spermatozoa
Omega-3
Antioxidant
Hormone
Equinos - Espermatozoides
Ômega-3
Antioxidante
Hormônios
description High seminal quality is important to achieve acceptable pregnancy rate following artificial insemination with cryopreserved semen in the equine industry. Docosahexaenoic acid (DHA) is an omega-3-polyunsaturated acid, which improves the integrity of the spermatozoa membrane during temperature changes. Insulin-like growth factor-I (IGF-I) is a protein hormone that helps mainly glucose to enter spermatozoa and it is an antioxidant. The aim of this study was to assess the effect of the in vitro addition of DHA in combination with IGF-I to frozen-thawed stallion semen. Three ejaculates from each of three Irish Sport Horse stallions were collected, the gel fraction was removed and semen was diluted in a 1:1 ratio using extender, centrifuged (1.000 g) for 10 minutes at 32 °C and ressuspended to 100 x 106 spermatozoa/mL in freezing extender. Semen was cooled to 4 °C, packed into 0.5 mL straws, frozen and stored under liquid nitrogen at -196 °C. Straws were thawed at 37 °C for 30 seconds, semen was diluted to 25 x 106 spermatozoa/mL and split in four treatments adding 0 or 1 ng of DHA /mL and 0 or 100 ng of IGF-I /mL: DHA0, DHA0 + IGF-I, DHA1 and DHA1 + IGF-I. Semen was incubated at 32 °C and after 30 minutes, total motility (TM), rapid progressive motility (PM), viability and acrosome integrity were assessed. After 60 and 120 minutes, TM and PM were assessed again. Post-thawed PM was higher (P < 0.05) when DHA1 + IGF-I was added, but there was no effect of the addition of DHA and IGF-I to TM, viability or acrosome integrity (P > 0.05).
publishDate 2019
dc.date.none.fl_str_mv 2019-06
2020-05-05T17:58:35Z
2020-05-05T17:58:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, D. M. et al. In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion sêmen. Revista Agrogeoambiental, Pouso Alegre, v. 11, n. 2, p. 127-138, jun. 2019.
http://repositorio.ufla.br/jspui/handle/1/40599
identifier_str_mv SILVA, D. M. et al. In vitro addition of DHA and IGF-I increases the progressive motility of cryopreserved stallion sêmen. Revista Agrogeoambiental, Pouso Alegre, v. 11, n. 2, p. 127-138, jun. 2019.
url http://repositorio.ufla.br/jspui/handle/1/40599
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Federal de Educação, Ciência e Tecnologia do Sul de Minas Gerais - IFSULDEMINAS
publisher.none.fl_str_mv Instituto Federal de Educação, Ciência e Tecnologia do Sul de Minas Gerais - IFSULDEMINAS
dc.source.none.fl_str_mv Revista Agrogeoambiental
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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