Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis

Detalhes bibliográficos
Autor(a) principal: Braga, Mariana Aparecida
Data de Publicação: 2022
Outros Autores: Abreu, Tatiane, Trento, Marcus Vinicius, Cesar, Pedro Henrique, Marques, Tamara Rezende, Thomasi, Sérgio Scherrer, Marcussi, Silvana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/50408
Resumo: Achyrocline satureioides is popularly known for its richness in phenolic compounds and medicinal properties (anti-inflammatory, analgesic, and hepatoprotective). The present study aimed at broadening the knowledge about the pharmacological potential exerted by the aqueous and ethanolic extracts of A. satureioides. These extracts were characterized by HPLC and tested for their modulatory action on phospholipases A2 and proteases of snake venoms. In addition, they were tested on the activities of digestive enzymes. Snake venoms were used as tools since they have enzymes with high functional and structural homology to human enzymes. The results demonstrate that the extracts of A. satureioides act as enzymatic inhibitors or potentiators, interfering in processes related to the hemostasis, such as coagulation and thrombus dissolution. In addition, the anti-genotoxic activity and inhibitions exerted on digestive enzymes suggests their potential use in the prevention and/or treatment of several pathologies. New studies could provide information on how the compounds present in the extracts and the different enzymes interact.
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spelling Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasisExtratos de Achyrocline satureioides (Lam.) DC atuando como moduladores enzimáticos: digestão, inflamação e hemostasiaComet assayProteases inhibitorsPhospholipases inhibitorsMedicinal plantsToxins as toolsEnsaio cometaInibidores de proteasesInibidores de fosfolipasesPlantas medicinaisToxinas como ferramentasAchyrocline satureioides is popularly known for its richness in phenolic compounds and medicinal properties (anti-inflammatory, analgesic, and hepatoprotective). The present study aimed at broadening the knowledge about the pharmacological potential exerted by the aqueous and ethanolic extracts of A. satureioides. These extracts were characterized by HPLC and tested for their modulatory action on phospholipases A2 and proteases of snake venoms. In addition, they were tested on the activities of digestive enzymes. Snake venoms were used as tools since they have enzymes with high functional and structural homology to human enzymes. The results demonstrate that the extracts of A. satureioides act as enzymatic inhibitors or potentiators, interfering in processes related to the hemostasis, such as coagulation and thrombus dissolution. In addition, the anti-genotoxic activity and inhibitions exerted on digestive enzymes suggests their potential use in the prevention and/or treatment of several pathologies. New studies could provide information on how the compounds present in the extracts and the different enzymes interact.A Achyrocline satureioides é popularmente conhecida por sua riqueza em compostos fenólicos e por suas propriedades medicinais (anti-inflamatória, analgésica e hepatoprotetora). No presente estudo, com o objetivo de ampliar o conhecimento sobre o potencial farmacológico exercido por esses extratos, os extratos aquoso e etanólico de A. satureioides foram caracterizados por HPLC e testados quanto à sua ação modulatória sobre as fosfolipases A2 e proteases de peçonhas de serpentes. Além disso, também foram testados em atividades de enzimas digestivas. As peçonhas de serpentes foram usadas como ferramentas por apresentarem enzimas com alta homologia funcional e estrutural às humanas. Os resultados demonstram que os extratos de A. satureioides atuam como inibidores ou potencializadores enzimáticos, interferindo em processos relacionados à hemostasia, como coagulação e dissolução do trombo. Além do mais, destacam seu potencial antigenotóxico e as inibições exercidas sobre as enzimas digestivas direcionando seu potencial de uso na prevenção e/ou tratamento de diversas patologias. Novos estudos poderão fornecer informações sobre os mecanismos de interação entre os compostos presentes nos extratos e as diferentes enzimas.Universidade Estadual de Londrina (UEL)2022-06-30T16:48:41Z2022-06-30T16:48:41Z2022-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBRAGA, M. A. et al. Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis. Semina: Ciências Biológicas e da Saúde, Londrina, v. 43, n. 1, p. 101-118, jan./jun. 2022. DOI: 10.5433/1679-0367.2022v43n1p101.http://repositorio.ufla.br/jspui/handle/1/50408Semina: Ciências Biológicas e da Saúdereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessBraga, Mariana AparecidaAbreu, TatianeTrento, Marcus ViniciusCesar, Pedro HenriqueMarques, Tamara RezendeThomasi, Sérgio ScherrerMarcussi, Silvanaeng2022-06-30T16:48:41Zoai:localhost:1/50408Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2022-06-30T16:48:41Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis
Extratos de Achyrocline satureioides (Lam.) DC atuando como moduladores enzimáticos: digestão, inflamação e hemostasia
title Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis
spellingShingle Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis
Braga, Mariana Aparecida
Comet assay
Proteases inhibitors
Phospholipases inhibitors
Medicinal plants
Toxins as tools
Ensaio cometa
Inibidores de proteases
Inibidores de fosfolipases
Plantas medicinais
Toxinas como ferramentas
title_short Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis
title_full Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis
title_fullStr Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis
title_full_unstemmed Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis
title_sort Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis
author Braga, Mariana Aparecida
author_facet Braga, Mariana Aparecida
Abreu, Tatiane
Trento, Marcus Vinicius
Cesar, Pedro Henrique
Marques, Tamara Rezende
Thomasi, Sérgio Scherrer
Marcussi, Silvana
author_role author
author2 Abreu, Tatiane
Trento, Marcus Vinicius
Cesar, Pedro Henrique
Marques, Tamara Rezende
Thomasi, Sérgio Scherrer
Marcussi, Silvana
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Braga, Mariana Aparecida
Abreu, Tatiane
Trento, Marcus Vinicius
Cesar, Pedro Henrique
Marques, Tamara Rezende
Thomasi, Sérgio Scherrer
Marcussi, Silvana
dc.subject.por.fl_str_mv Comet assay
Proteases inhibitors
Phospholipases inhibitors
Medicinal plants
Toxins as tools
Ensaio cometa
Inibidores de proteases
Inibidores de fosfolipases
Plantas medicinais
Toxinas como ferramentas
topic Comet assay
Proteases inhibitors
Phospholipases inhibitors
Medicinal plants
Toxins as tools
Ensaio cometa
Inibidores de proteases
Inibidores de fosfolipases
Plantas medicinais
Toxinas como ferramentas
description Achyrocline satureioides is popularly known for its richness in phenolic compounds and medicinal properties (anti-inflammatory, analgesic, and hepatoprotective). The present study aimed at broadening the knowledge about the pharmacological potential exerted by the aqueous and ethanolic extracts of A. satureioides. These extracts were characterized by HPLC and tested for their modulatory action on phospholipases A2 and proteases of snake venoms. In addition, they were tested on the activities of digestive enzymes. Snake venoms were used as tools since they have enzymes with high functional and structural homology to human enzymes. The results demonstrate that the extracts of A. satureioides act as enzymatic inhibitors or potentiators, interfering in processes related to the hemostasis, such as coagulation and thrombus dissolution. In addition, the anti-genotoxic activity and inhibitions exerted on digestive enzymes suggests their potential use in the prevention and/or treatment of several pathologies. New studies could provide information on how the compounds present in the extracts and the different enzymes interact.
publishDate 2022
dc.date.none.fl_str_mv 2022-06-30T16:48:41Z
2022-06-30T16:48:41Z
2022-06
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BRAGA, M. A. et al. Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis. Semina: Ciências Biológicas e da Saúde, Londrina, v. 43, n. 1, p. 101-118, jan./jun. 2022. DOI: 10.5433/1679-0367.2022v43n1p101.
http://repositorio.ufla.br/jspui/handle/1/50408
identifier_str_mv BRAGA, M. A. et al. Achyrocline satureioides (Lam.) DC extracts acting as enzyme modulators: digestion, inflammation, and hemostasis. Semina: Ciências Biológicas e da Saúde, Londrina, v. 43, n. 1, p. 101-118, jan./jun. 2022. DOI: 10.5433/1679-0367.2022v43n1p101.
url http://repositorio.ufla.br/jspui/handle/1/50408
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Londrina (UEL)
publisher.none.fl_str_mv Universidade Estadual de Londrina (UEL)
dc.source.none.fl_str_mv Semina: Ciências Biológicas e da Saúde
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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