Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/30320 |
Resumo: | A great number of species and individuals of scandent legumes establishing symbiosis with nitrogen fixing bacteria occurs in the Amazon Forest. These symbiosis probably play an important role in contributing to nitrogen incorporation in this ecossystem. The objectives of this study were to evaluate the growth of eight species of scandent legumes in five nursery substrates; to compare nodulation with rhizobia strains introduced or native to these substrates; and to characterize phenotypically and genetically these rhizobia. The experiment was carried out in a completely randomized design with five replications. Five to seven months after seedling emergency, according to the legume species, growth and nodulation parameters were determined. Rhizobia identification of strains was carried out by 16S rRNA gene partial sequencing. The survival of seedlings after the transplanting varied from 93 to 98%, in Ultisol (Argissolo in Brazilian classification), collected in primary forest, and fertilized with all nutrients, except nitrogen (ULTfert); and in a clay and sand mixture, in a ratio 3:2 (CONV), respectively. Species with height superior to 30 cm, in general, grew better in substrates with higher fertility: ULTfert and Humic Gley soil (HG). Seven out of the eight species were able to nodulate. The percentage of nodulation per substrate was: SAND, washed sand with mixed inoculum of 100 rhizobia strains plus fertilization (100), HG (80), CONV (100), ULT, A-horizon of red-yellow Ultisol collected in the Ducke Forest Reserve (Manaus) (44), and ULTfert (55%). Bradyrhizobium spp. were isolated from nodules of all species and substrates. Burkolderia fungorum was isolated from Dalbergia inundata. For Dalbergia riedelli and Dalbergia inundata, this is the first report on the identification of symbiotic strains. Scandent legumes present high survival of seedlings in nursery, and develop better in substrates with higher fertility, and generally present symbiosis with Bradyrhizobium. |
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Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon regionCrescimento e simbiose com rizóbios em casa de vegetação de espécies de Leguminosae escandentes nativas da região AmazônicaBactérias fixadoras de nitrogênioLeguminosas escandentesBradyrhizobium - SimbioseBiological nitrogen fixationThick legumesBradyrhizobium - SymbiosisA great number of species and individuals of scandent legumes establishing symbiosis with nitrogen fixing bacteria occurs in the Amazon Forest. These symbiosis probably play an important role in contributing to nitrogen incorporation in this ecossystem. The objectives of this study were to evaluate the growth of eight species of scandent legumes in five nursery substrates; to compare nodulation with rhizobia strains introduced or native to these substrates; and to characterize phenotypically and genetically these rhizobia. The experiment was carried out in a completely randomized design with five replications. Five to seven months after seedling emergency, according to the legume species, growth and nodulation parameters were determined. Rhizobia identification of strains was carried out by 16S rRNA gene partial sequencing. The survival of seedlings after the transplanting varied from 93 to 98%, in Ultisol (Argissolo in Brazilian classification), collected in primary forest, and fertilized with all nutrients, except nitrogen (ULTfert); and in a clay and sand mixture, in a ratio 3:2 (CONV), respectively. Species with height superior to 30 cm, in general, grew better in substrates with higher fertility: ULTfert and Humic Gley soil (HG). Seven out of the eight species were able to nodulate. The percentage of nodulation per substrate was: SAND, washed sand with mixed inoculum of 100 rhizobia strains plus fertilization (100), HG (80), CONV (100), ULT, A-horizon of red-yellow Ultisol collected in the Ducke Forest Reserve (Manaus) (44), and ULTfert (55%). Bradyrhizobium spp. were isolated from nodules of all species and substrates. Burkolderia fungorum was isolated from Dalbergia inundata. For Dalbergia riedelli and Dalbergia inundata, this is the first report on the identification of symbiotic strains. Scandent legumes present high survival of seedlings in nursery, and develop better in substrates with higher fertility, and generally present symbiosis with Bradyrhizobium.Um grande número de espécies e indivíduos de leguminosas escandentes estabelecendo simbiose com bactérias fixadoras de nitrogênio ocorrem na floresta Amazônica. Estas simbioses provavelmente desempenham importante papel, contribuindo para incorporação de nitrogênio neste ecossistema. Os objetivos deste trabalho foram avaliar o crescimento de oito espécies de leguminosas escandentes em cinco substratos no viveiro; comparar a nodulação com as estirpes de rizóbio introduzidas ou nativas destes substratos; e caracterizar esses rizóbios fenotípica e geneticamente. O experimento foi conduzido em delineamento inteiramente casualizado com cinco repetições. Cinco a sete meses após a emergência das mudas parâmetros de crescimento e nodulação foram determinados. A identificação das estirpes de rizóbios foi feita pelo sequenciamento parcial do gene 16S rRNA. A sobrevivência das mudas após o transplante variou de 93 a 98%, em um Argissolo (Ultisol de acordo com a classificação da USDA), coletado em floresta primária e adubado com todos os nutrientes, exceto nitrogênio (ULTfert), e na mistura argila e areia, na proporção 3:2 (CONV), respectivamente. Espécies que apresentaram altura superior a 30 cm, no geral, cresceram melhor nos substratos de maior fertilidade: ULTfert e solo Glei Húmico (GH). Das oito espécies estudadas, sete nodularam. A porcentagem de nodulação por substrato foi: AREIA, areia lavada com inóculo misto de 100 estirpes de rizóbio mais adubação (100), GH (80), CONV (100), ULT, horizonte A de Argissolo vermelho-amarelo coletado na Reserva Florestal Ducke (Manaus) (44) e ULTfert (55%). Bradyrhizobium spp. foram isolados de nódulos de todas as espécies e substratos. Burkolderia fungorum foi isolada de Dalbergia inundata. Este é o primeiro relato de identificação de estirpes simbióticas para Dalbergia riedelli e Dalbergia inundata. Leguminosas escandentes apresentaram alta sobrevivência de mudas em casa de vegetação e desenvolveram-se melhor em substratos com maior fertilidade e, geralmente, estabeleceram simbiose com Bradyrhizobium.Instituto Nacional de Pesquisas da Amazônia2018-08-31T11:12:14Z2018-08-31T11:12:14Z2016-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMOREIRA, F. M. de S. et al. Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region. Acta Amazonica, Manaus, v. 46, n. 4, p. 367-376, 2016.http://repositorio.ufla.br/jspui/handle/1/30320Acta Amazonicareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMoreira, Fatima Maria de SouzaCoelho, Katia PereiraRibeiro, Paula Rose de AlmeidaGuimarães, Amanda Azariaseng2018-08-31T11:12:15Zoai:localhost:1/30320Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-08-31T11:12:15Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region Crescimento e simbiose com rizóbios em casa de vegetação de espécies de Leguminosae escandentes nativas da região Amazônica |
title |
Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region |
spellingShingle |
Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region Moreira, Fatima Maria de Souza Bactérias fixadoras de nitrogênio Leguminosas escandentes Bradyrhizobium - Simbiose Biological nitrogen fixation Thick legumes Bradyrhizobium - Symbiosis |
title_short |
Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region |
title_full |
Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region |
title_fullStr |
Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region |
title_full_unstemmed |
Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region |
title_sort |
Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region |
author |
Moreira, Fatima Maria de Souza |
author_facet |
Moreira, Fatima Maria de Souza Coelho, Katia Pereira Ribeiro, Paula Rose de Almeida Guimarães, Amanda Azarias |
author_role |
author |
author2 |
Coelho, Katia Pereira Ribeiro, Paula Rose de Almeida Guimarães, Amanda Azarias |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Moreira, Fatima Maria de Souza Coelho, Katia Pereira Ribeiro, Paula Rose de Almeida Guimarães, Amanda Azarias |
dc.subject.por.fl_str_mv |
Bactérias fixadoras de nitrogênio Leguminosas escandentes Bradyrhizobium - Simbiose Biological nitrogen fixation Thick legumes Bradyrhizobium - Symbiosis |
topic |
Bactérias fixadoras de nitrogênio Leguminosas escandentes Bradyrhizobium - Simbiose Biological nitrogen fixation Thick legumes Bradyrhizobium - Symbiosis |
description |
A great number of species and individuals of scandent legumes establishing symbiosis with nitrogen fixing bacteria occurs in the Amazon Forest. These symbiosis probably play an important role in contributing to nitrogen incorporation in this ecossystem. The objectives of this study were to evaluate the growth of eight species of scandent legumes in five nursery substrates; to compare nodulation with rhizobia strains introduced or native to these substrates; and to characterize phenotypically and genetically these rhizobia. The experiment was carried out in a completely randomized design with five replications. Five to seven months after seedling emergency, according to the legume species, growth and nodulation parameters were determined. Rhizobia identification of strains was carried out by 16S rRNA gene partial sequencing. The survival of seedlings after the transplanting varied from 93 to 98%, in Ultisol (Argissolo in Brazilian classification), collected in primary forest, and fertilized with all nutrients, except nitrogen (ULTfert); and in a clay and sand mixture, in a ratio 3:2 (CONV), respectively. Species with height superior to 30 cm, in general, grew better in substrates with higher fertility: ULTfert and Humic Gley soil (HG). Seven out of the eight species were able to nodulate. The percentage of nodulation per substrate was: SAND, washed sand with mixed inoculum of 100 rhizobia strains plus fertilization (100), HG (80), CONV (100), ULT, A-horizon of red-yellow Ultisol collected in the Ducke Forest Reserve (Manaus) (44), and ULTfert (55%). Bradyrhizobium spp. were isolated from nodules of all species and substrates. Burkolderia fungorum was isolated from Dalbergia inundata. For Dalbergia riedelli and Dalbergia inundata, this is the first report on the identification of symbiotic strains. Scandent legumes present high survival of seedlings in nursery, and develop better in substrates with higher fertility, and generally present symbiosis with Bradyrhizobium. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10 2018-08-31T11:12:14Z 2018-08-31T11:12:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MOREIRA, F. M. de S. et al. Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region. Acta Amazonica, Manaus, v. 46, n. 4, p. 367-376, 2016. http://repositorio.ufla.br/jspui/handle/1/30320 |
identifier_str_mv |
MOREIRA, F. M. de S. et al. Nursery growth and rhizobia symbiosis of scandent Leguminosae species native to the Amazon region. Acta Amazonica, Manaus, v. 46, n. 4, p. 367-376, 2016. |
url |
http://repositorio.ufla.br/jspui/handle/1/30320 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
publisher.none.fl_str_mv |
Instituto Nacional de Pesquisas da Amazônia |
dc.source.none.fl_str_mv |
Acta Amazonica reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835092420132864 |