Genetic similarity between coriander genotypes using ISSR markers
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/37581 |
Resumo: | With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line) using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-9299. The genetic similarity between the cultivars was estimated using 227 banded regions of ISSR molecular markers. The UBC 897 oligonucleotide generated the highest number of fragments (16), resulting in a higher polymorphism. The results indicate that the twenty-nine oligonucleotides chosen were satisfactory for detecting polymorphism. Based on the grouping analysis determined from the similarity data, there were two groups and two sub-groups. The calculated similarity for the genotypes varied from 52 to 75%. The lowest similarity was observed between Português and Verdão, at 52%. The highest similarity was found between Português and Palmeira, at 75%. The ISSR is efficient for identifying DNA polymorphism in coriander. |
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Genetic similarity between coriander genotypes using ISSR markersSimilaridade genética entre genótipos de coentro por marcadores ISSRCoriandrum sativum L.Molecular characterizationMicrosatellitesCondimentsCaracterização molecularMicrossatélitesCondimentaresWith the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line) using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-9299. The genetic similarity between the cultivars was estimated using 227 banded regions of ISSR molecular markers. The UBC 897 oligonucleotide generated the highest number of fragments (16), resulting in a higher polymorphism. The results indicate that the twenty-nine oligonucleotides chosen were satisfactory for detecting polymorphism. Based on the grouping analysis determined from the similarity data, there were two groups and two sub-groups. The calculated similarity for the genotypes varied from 52 to 75%. The lowest similarity was observed between Português and Verdão, at 52%. The highest similarity was found between Português and Palmeira, at 75%. The ISSR is efficient for identifying DNA polymorphism in coriander.Com o surgimento de novas cultivares, uma caracterização genética precisa é essencial, visando à utilização em programas de melhoramento ou para fins de registros e ou proteção de cultivares. Marcadores moleculares vêm complementando a caracterização morfológica e agronômica tradicional, uma vez que são virtualmente ilimitados, cobrem todo o genoma e não são influenciados pelo ambiente. A caracterização genética constitui a base para trabalhos de estimativas de similaridade genética. Portanto, este trabalho teve como objetivo avaliar a similaridade genética entre dez genótipos de coentro (nove cultivares e uma linhagem) por meio de marcadores ISSR. As cultivares utilizadas foram Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão e a linhagem experimental HTV-9299. A similaridade genética entre as cultivares foi estimada com base nos marcadores moleculares de ISSR, utilizando-se 227 regiões de bandas de ISSR. O oligonucleotídeo UBC 897 gerou o maior número de fragmentos (16), resultando em um maior polimorfismo. Os resultados indicam que os vinte e nove oligonucleotídeos escolhidos foram satisfatórios para detecção de polimorfismo. Como resultado da análise de agrupamento a partir dos dados de similaridade, verificou-se a presença de dois grupos e dois subgrupos. A similaridade calculada para os genótipos variou de 52 a 75%. A menor similaridade ficou entre Português e Verdão, com 52%. Já a maior similaridade foi obtida entre Português e Palmeira com 75%. O ISSR é eficiente na identificação de polimorfismo de DNA em coentro.Associação Brasileira de Horticultura (ABH)2019-11-08T13:05:58Z2019-11-08T13:05:58Z2011-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMELO, R. de A. et al. Genetic similarity between coriander genotypes using ISSR markers. Horticultura Brasileira, Brasília, DF, v. 29, n. 4, p. 526-530, out./dez. 2011. DOI: 10.1590/S0102-05362011000400014.http://repositorio.ufla.br/jspui/handle/1/37581Horticultura Brasileirareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessMelo, Roberto de A.Resende, Luciane V.Menezes, DimasBeck, Ana Paula A.Costa, José Carlos daCoutinho, Alisson E.Nascimento, Ana Verônica S. doeng2023-05-26T18:50:14Zoai:localhost:1/37581Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:50:14Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Genetic similarity between coriander genotypes using ISSR markers Similaridade genética entre genótipos de coentro por marcadores ISSR |
title |
Genetic similarity between coriander genotypes using ISSR markers |
spellingShingle |
Genetic similarity between coriander genotypes using ISSR markers Melo, Roberto de A. Coriandrum sativum L. Molecular characterization Microsatellites Condiments Caracterização molecular Microssatélites Condimentares |
title_short |
Genetic similarity between coriander genotypes using ISSR markers |
title_full |
Genetic similarity between coriander genotypes using ISSR markers |
title_fullStr |
Genetic similarity between coriander genotypes using ISSR markers |
title_full_unstemmed |
Genetic similarity between coriander genotypes using ISSR markers |
title_sort |
Genetic similarity between coriander genotypes using ISSR markers |
author |
Melo, Roberto de A. |
author_facet |
Melo, Roberto de A. Resende, Luciane V. Menezes, Dimas Beck, Ana Paula A. Costa, José Carlos da Coutinho, Alisson E. Nascimento, Ana Verônica S. do |
author_role |
author |
author2 |
Resende, Luciane V. Menezes, Dimas Beck, Ana Paula A. Costa, José Carlos da Coutinho, Alisson E. Nascimento, Ana Verônica S. do |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Melo, Roberto de A. Resende, Luciane V. Menezes, Dimas Beck, Ana Paula A. Costa, José Carlos da Coutinho, Alisson E. Nascimento, Ana Verônica S. do |
dc.subject.por.fl_str_mv |
Coriandrum sativum L. Molecular characterization Microsatellites Condiments Caracterização molecular Microssatélites Condimentares |
topic |
Coriandrum sativum L. Molecular characterization Microsatellites Condiments Caracterização molecular Microssatélites Condimentares |
description |
With the development of new cultivars, a precise genetic characterization is essential for improvement programs or for cultivar registration and protection. Molecular markers have been complementing the traditional morphological and agronomic characterization techniques because they are virtually unlimited, cover the whole genome and are not environmentally influenced. Genetic characterization constitutes the basis for studies involving estimates of genetic similarity. Therefore, the objective of the present study was to evaluate the genetic similarity between ten coriander genotypes (nine cultivars and one line) using ISSR markers. The cultivars used were: Americano, Asteca, Palmeira, Português, Santo, Supéria, Tabocas, Tapacurá, Verdão and the experimental line HTV-9299. The genetic similarity between the cultivars was estimated using 227 banded regions of ISSR molecular markers. The UBC 897 oligonucleotide generated the highest number of fragments (16), resulting in a higher polymorphism. The results indicate that the twenty-nine oligonucleotides chosen were satisfactory for detecting polymorphism. Based on the grouping analysis determined from the similarity data, there were two groups and two sub-groups. The calculated similarity for the genotypes varied from 52 to 75%. The lowest similarity was observed between Português and Verdão, at 52%. The highest similarity was found between Português and Palmeira, at 75%. The ISSR is efficient for identifying DNA polymorphism in coriander. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12 2019-11-08T13:05:58Z 2019-11-08T13:05:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MELO, R. de A. et al. Genetic similarity between coriander genotypes using ISSR markers. Horticultura Brasileira, Brasília, DF, v. 29, n. 4, p. 526-530, out./dez. 2011. DOI: 10.1590/S0102-05362011000400014. http://repositorio.ufla.br/jspui/handle/1/37581 |
identifier_str_mv |
MELO, R. de A. et al. Genetic similarity between coriander genotypes using ISSR markers. Horticultura Brasileira, Brasília, DF, v. 29, n. 4, p. 526-530, out./dez. 2011. DOI: 10.1590/S0102-05362011000400014. |
url |
http://repositorio.ufla.br/jspui/handle/1/37581 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Horticultura (ABH) |
publisher.none.fl_str_mv |
Associação Brasileira de Horticultura (ABH) |
dc.source.none.fl_str_mv |
Horticultura Brasileira reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835201781366784 |