Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/34504 |
Resumo: | In this work, it was developed novel bioreactor consisting of Aspergillus niger lipase (ANL) entrapped in a polyacrylamide-based supermacroporous monolithic cryogel. Bioreactor performance in catalyzing the flavor ester butyl butyrate was optimized as a function of temperature (°C), substrate molar ratio (butyric acid: n-butanol) and added water (% (v/v)). The bioreactor was also applied to interesterification reactions. The maximum esterification yield (46%) for butyl butyrate synthesis was found at 40 °C, with a butyric acid:n-butanol molar ratio of 1:1.43 and added water of 65% (v/v). The bioreactor proved to be reusable with four consecutive reuses in the esterification reaction, with a residual esterification yield of 64.6% and volumetric activity of 31.6 mmol/mL·h. A high interesterification yield (17.35% in 24 h) was achieved when the substrate concentrations of tripalmitin and triolein were both 15 mmol/L, 35% added water (v/v) and temperature of 40 °C. These results showed that the bioreactor is a promising biocatalyst for biotechnological applications. |
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Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactionsDesarollo de un biorreactor compuesto por lipasa atrapada em un criogel monolítico para las reacciones de esterificación e interesterificaciónCryogelLipaseEntrapmentButyl butyrateTripalmitinAprisionamentoButirato de butiloTripalmitinaIn this work, it was developed novel bioreactor consisting of Aspergillus niger lipase (ANL) entrapped in a polyacrylamide-based supermacroporous monolithic cryogel. Bioreactor performance in catalyzing the flavor ester butyl butyrate was optimized as a function of temperature (°C), substrate molar ratio (butyric acid: n-butanol) and added water (% (v/v)). The bioreactor was also applied to interesterification reactions. The maximum esterification yield (46%) for butyl butyrate synthesis was found at 40 °C, with a butyric acid:n-butanol molar ratio of 1:1.43 and added water of 65% (v/v). The bioreactor proved to be reusable with four consecutive reuses in the esterification reaction, with a residual esterification yield of 64.6% and volumetric activity of 31.6 mmol/mL·h. A high interesterification yield (17.35% in 24 h) was achieved when the substrate concentrations of tripalmitin and triolein were both 15 mmol/L, 35% added water (v/v) and temperature of 40 °C. These results showed that the bioreactor is a promising biocatalyst for biotechnological applications.En este trabajo se desarroll ́o un nuevo biorreactor compuesto por lipasa deAspergillus niger(ANL) atrapada en un criogelmonol ́ıtico supermacroporoso basado en poliacrilamida. El rendimiento del biorreactor en la catalizaci ́on del ́ester aromatizantebutirato de butilo se optimiz ́o en funci ́on de la temperatura (°C), de la relaci ́on molar del sustrato ( ́acido but ́ırico: n-butanol)y del agua a ̃nadida (% (v/v)). El biorreactor tambi ́en se aplic ́o a reacciones de interesterificaci ́on. El rendimiento m ́aximo deesterificaci ́on (46%) para la s ́ıntesis de butirato de butilo se encontr ́o a 40 °C, con una relaci ́on molar de ́acido but ́ırico: n-butanol de 1: 1,43 y agua a ̃nadida de 65% (v/v). El biorreactor demostr ́o ser reutilizable con cuatro reutilizaciones consecutivasen la reacci ́on de esterificaci ́on, con un rendimiento de esterificaci ́on residual del 64,6% y una actividad volum ́etrica de 31,6mmol/mL·h. Se logr ́o un alto rendimiento de interesterificaci ́on (17,35% en 24 hrs) cuando ambas concentraciones, la de sustratode tripalmitina y la de triole ́ına, fueron de 15 mmol/L, con 35% de agua a ̃nadida (v/v) y a una temperatura de 40 ºC. Estosresultados mostraron que el biorreactor es un biocatalizador prometedor para aplicaciones biotecnol ́ogicas.Palabras clave: criogel, lipasa, atrapamiento, butirato de butilo, tripalmitina.Universidad Autónoma Metropolitana2019-06-03T11:52:53Z2019-06-03T11:52:53Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfVERÍSSIMO, L. A. A. et al. Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions. Revista Mexicana de Ingeniería Química, [S. l.], v. 17, n. 1, p. 177-188, 2018.http://repositorio.ufla.br/jspui/handle/1/34504Revista Mexicana de Ingeniería Químicareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessVeríssimo, L. A. A.Mól, P. C. G.Soares, W. C. L.Minim, V. P. R.Hespanhol, M. C.Minim, L. A.eng2019-06-03T11:52:54Zoai:localhost:1/34504Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-06-03T11:52:54Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions Desarollo de un biorreactor compuesto por lipasa atrapada em un criogel monolítico para las reacciones de esterificación e interesterificación |
title |
Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions |
spellingShingle |
Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions Veríssimo, L. A. A. Cryogel Lipase Entrapment Butyl butyrate Tripalmitin Aprisionamento Butirato de butilo Tripalmitina |
title_short |
Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions |
title_full |
Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions |
title_fullStr |
Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions |
title_full_unstemmed |
Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions |
title_sort |
Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions |
author |
Veríssimo, L. A. A. |
author_facet |
Veríssimo, L. A. A. Mól, P. C. G. Soares, W. C. L. Minim, V. P. R. Hespanhol, M. C. Minim, L. A. |
author_role |
author |
author2 |
Mól, P. C. G. Soares, W. C. L. Minim, V. P. R. Hespanhol, M. C. Minim, L. A. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Veríssimo, L. A. A. Mól, P. C. G. Soares, W. C. L. Minim, V. P. R. Hespanhol, M. C. Minim, L. A. |
dc.subject.por.fl_str_mv |
Cryogel Lipase Entrapment Butyl butyrate Tripalmitin Aprisionamento Butirato de butilo Tripalmitina |
topic |
Cryogel Lipase Entrapment Butyl butyrate Tripalmitin Aprisionamento Butirato de butilo Tripalmitina |
description |
In this work, it was developed novel bioreactor consisting of Aspergillus niger lipase (ANL) entrapped in a polyacrylamide-based supermacroporous monolithic cryogel. Bioreactor performance in catalyzing the flavor ester butyl butyrate was optimized as a function of temperature (°C), substrate molar ratio (butyric acid: n-butanol) and added water (% (v/v)). The bioreactor was also applied to interesterification reactions. The maximum esterification yield (46%) for butyl butyrate synthesis was found at 40 °C, with a butyric acid:n-butanol molar ratio of 1:1.43 and added water of 65% (v/v). The bioreactor proved to be reusable with four consecutive reuses in the esterification reaction, with a residual esterification yield of 64.6% and volumetric activity of 31.6 mmol/mL·h. A high interesterification yield (17.35% in 24 h) was achieved when the substrate concentrations of tripalmitin and triolein were both 15 mmol/L, 35% added water (v/v) and temperature of 40 °C. These results showed that the bioreactor is a promising biocatalyst for biotechnological applications. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2019-06-03T11:52:53Z 2019-06-03T11:52:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VERÍSSIMO, L. A. A. et al. Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions. Revista Mexicana de Ingeniería Química, [S. l.], v. 17, n. 1, p. 177-188, 2018. http://repositorio.ufla.br/jspui/handle/1/34504 |
identifier_str_mv |
VERÍSSIMO, L. A. A. et al. Development of a bioreactor based on lipase entrapped in a monolithic cryogel for esterification and interesterification reactions. Revista Mexicana de Ingeniería Química, [S. l.], v. 17, n. 1, p. 177-188, 2018. |
url |
http://repositorio.ufla.br/jspui/handle/1/34504 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidad Autónoma Metropolitana |
publisher.none.fl_str_mv |
Universidad Autónoma Metropolitana |
dc.source.none.fl_str_mv |
Revista Mexicana de Ingeniería Química reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1815438971667218432 |