Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/50671 |
Resumo: | Due to the intense exploitation of its essential oil over the years, Ocotea odorifera was included in the endangered species in Brazil. In this context, the micro-propagation technique is an alternative to sexual propagation, and consequently, it favors the conservation of the species. Thus, the objective of this study was to produce seedlings of O. odorifera through the micro-propagation technique. Seed collection was carried out in mother trees distributed in a natural stand. In vitro cultivation was divided into three phases: (i) in vitro germination and establishment; (ii) elongation and rooting and (iii) acclimatization. For explant disinfestation, a 3x2 factorial was used: three sources of propagules (seed, embryo and nodal segment) combined with two immersion times in NaClO (50%) (5 and 10 min), totaling 6 treatments. In the in vitro establishment, two culture media (MS supplemented with ANA and BAP) combined with or without the addition of activated charcoal were tested. In the elongation and rooting phase, the presence and absence of activated charcoal in the development of shoots was evaluated. For the acclimatization of seedlings and shoots, two ex vitro conditions were evaluated: in a growth room environment and in a nursery. We found significant differences between the evaluated treatments. The embryo-type propagule source and disinfested with 50% NaClO for 10 minutes showed the best morphological characteristics of germination and development. The addition of charcoal to basic MS medium, and free from plant regulators, reduced tissue oxidation in the elongation and rooting phase. Shoots of Ocotea odorifera developed in vitro and adapted well to ex vitro acclimatization conditions. Therefore, the micro-propagation protocol used in this work was efficient, providing the production of healthy and suitable seedlings for field conditions. |
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Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation techniqueProdução de mudas de Ocotea odorifera (Vell.) Rohwer por meio da técnica de micropropagaçãoCanela-sassafrásPropagationTissue cultureSeedsPropagaçãoCultura de tecidosSementesDue to the intense exploitation of its essential oil over the years, Ocotea odorifera was included in the endangered species in Brazil. In this context, the micro-propagation technique is an alternative to sexual propagation, and consequently, it favors the conservation of the species. Thus, the objective of this study was to produce seedlings of O. odorifera through the micro-propagation technique. Seed collection was carried out in mother trees distributed in a natural stand. In vitro cultivation was divided into three phases: (i) in vitro germination and establishment; (ii) elongation and rooting and (iii) acclimatization. For explant disinfestation, a 3x2 factorial was used: three sources of propagules (seed, embryo and nodal segment) combined with two immersion times in NaClO (50%) (5 and 10 min), totaling 6 treatments. In the in vitro establishment, two culture media (MS supplemented with ANA and BAP) combined with or without the addition of activated charcoal were tested. In the elongation and rooting phase, the presence and absence of activated charcoal in the development of shoots was evaluated. For the acclimatization of seedlings and shoots, two ex vitro conditions were evaluated: in a growth room environment and in a nursery. We found significant differences between the evaluated treatments. The embryo-type propagule source and disinfested with 50% NaClO for 10 minutes showed the best morphological characteristics of germination and development. The addition of charcoal to basic MS medium, and free from plant regulators, reduced tissue oxidation in the elongation and rooting phase. Shoots of Ocotea odorifera developed in vitro and adapted well to ex vitro acclimatization conditions. Therefore, the micro-propagation protocol used in this work was efficient, providing the production of healthy and suitable seedlings for field conditions.Devido à intensa exploração de óleo essencial ao longo dos anos, Ocotea odorifera foi incluída em espécies ameaçadas de extinção no Brasil. Diante desse contexto, a técnica de micropropagação é uma alternativa à propagação sexuada, e consequentemente, favorece a conservação da espécie. Desta forma, o objetivo deste estudo foi produzir mudas de O. odorifera por meio da técnica de micropropagação. A coleta de sementes foi realizada em árvores matrizes distribuídas em povoamento natural. O cultivo in vitro foi dividido em três fases: (i) germinação e estabelecimento in vitro; (ii) alongamento e enraizamento e (iii) aclimatização. Na desinfestação dos explantes utilizou-se um fatorial 3x2: três fontes de propágulo (semente, embrião e segmento nodal) combinado com dois tempo de imersão em NaClO (50%) (5 e 10 min), totalizando 6 tratamentos. No estabelecimento in vitro testaram-se dois meio de cultura (MS suplementado com ANA e BAP) combinados com ou sem a adição de carvão ativado. Na fase de alongamento e enraizamento avaliou-se a presença e ausência de carvão ativado no desenvolvimento das brotações. Para a aclimatação das plântulas e brotações avaliaram-se duas condições ex vitro, em ambiente de sala de crescimento e em viveiro. Houve diferenças significativas entre os tratamentos avaliados: a fonte de propágulo do tipo embrião e desinfestado com NaClO a 50% por 10 minutos apresentou as melhores características morfológicas de germinação e desenvolvimento. A adição do carvão ao meio MS básico, e isento de fitorreguladores, reduziu a oxidação de tecidos na fase de alongamento e enraizamento. As brotações de Ocotea odorifera desenvolvidas in vitro, adaptaram-se bem às condições de aclimatização ex vitro. Portanto, o protocolo de micropropagação utilizado no presente trabalho foi eficiente, proporcionando a produção de mudas sadias e aptas para condução a campo.Instituto de Pesquisas e Estudos Florestais (IPEF)2022-07-20T22:31:31Z2022-07-20T22:31:31Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSANTOS, J. A. et al. Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique. Scientia Forestalis, Piracicaba, v. 50, e3759, 2022. DOI: https://doi.org/10.18671/scifor.v50.04.http://repositorio.ufla.br/jspui/handle/1/50671Scientia Forestalisreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSantos, Juscelina ArcanjoPaula, Natália RibeiroSouza, Denys Matheus Santana CostaCarvalho, Dulcinéia deBrondani, Gilvano Eblingeng2022-07-20T22:31:54Zoai:localhost:1/50671Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2022-07-20T22:31:54Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique Produção de mudas de Ocotea odorifera (Vell.) Rohwer por meio da técnica de micropropagação |
title |
Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique |
spellingShingle |
Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique Santos, Juscelina Arcanjo Canela-sassafrás Propagation Tissue culture Seeds Propagação Cultura de tecidos Sementes |
title_short |
Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique |
title_full |
Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique |
title_fullStr |
Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique |
title_full_unstemmed |
Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique |
title_sort |
Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique |
author |
Santos, Juscelina Arcanjo |
author_facet |
Santos, Juscelina Arcanjo Paula, Natália Ribeiro Souza, Denys Matheus Santana Costa Carvalho, Dulcinéia de Brondani, Gilvano Ebling |
author_role |
author |
author2 |
Paula, Natália Ribeiro Souza, Denys Matheus Santana Costa Carvalho, Dulcinéia de Brondani, Gilvano Ebling |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Santos, Juscelina Arcanjo Paula, Natália Ribeiro Souza, Denys Matheus Santana Costa Carvalho, Dulcinéia de Brondani, Gilvano Ebling |
dc.subject.por.fl_str_mv |
Canela-sassafrás Propagation Tissue culture Seeds Propagação Cultura de tecidos Sementes |
topic |
Canela-sassafrás Propagation Tissue culture Seeds Propagação Cultura de tecidos Sementes |
description |
Due to the intense exploitation of its essential oil over the years, Ocotea odorifera was included in the endangered species in Brazil. In this context, the micro-propagation technique is an alternative to sexual propagation, and consequently, it favors the conservation of the species. Thus, the objective of this study was to produce seedlings of O. odorifera through the micro-propagation technique. Seed collection was carried out in mother trees distributed in a natural stand. In vitro cultivation was divided into three phases: (i) in vitro germination and establishment; (ii) elongation and rooting and (iii) acclimatization. For explant disinfestation, a 3x2 factorial was used: three sources of propagules (seed, embryo and nodal segment) combined with two immersion times in NaClO (50%) (5 and 10 min), totaling 6 treatments. In the in vitro establishment, two culture media (MS supplemented with ANA and BAP) combined with or without the addition of activated charcoal were tested. In the elongation and rooting phase, the presence and absence of activated charcoal in the development of shoots was evaluated. For the acclimatization of seedlings and shoots, two ex vitro conditions were evaluated: in a growth room environment and in a nursery. We found significant differences between the evaluated treatments. The embryo-type propagule source and disinfested with 50% NaClO for 10 minutes showed the best morphological characteristics of germination and development. The addition of charcoal to basic MS medium, and free from plant regulators, reduced tissue oxidation in the elongation and rooting phase. Shoots of Ocotea odorifera developed in vitro and adapted well to ex vitro acclimatization conditions. Therefore, the micro-propagation protocol used in this work was efficient, providing the production of healthy and suitable seedlings for field conditions. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-20T22:31:31Z 2022-07-20T22:31:31Z 2022 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SANTOS, J. A. et al. Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique. Scientia Forestalis, Piracicaba, v. 50, e3759, 2022. DOI: https://doi.org/10.18671/scifor.v50.04. http://repositorio.ufla.br/jspui/handle/1/50671 |
identifier_str_mv |
SANTOS, J. A. et al. Plant production of Ocotea odorifera (Vell.) Rohwer by the micro-propagation technique. Scientia Forestalis, Piracicaba, v. 50, e3759, 2022. DOI: https://doi.org/10.18671/scifor.v50.04. |
url |
http://repositorio.ufla.br/jspui/handle/1/50671 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Instituto de Pesquisas e Estudos Florestais (IPEF) |
publisher.none.fl_str_mv |
Instituto de Pesquisas e Estudos Florestais (IPEF) |
dc.source.none.fl_str_mv |
Scientia Forestalis reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835081059860480 |