Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/11827 |
Resumo: | Brazil has the largest global diversity index, however we have witnessed the high percentage of environmental devastation. Maintaining species in the laboratory, together with innovation, are the best alternative to maintai n that diversity. The soil is considered to be a mosaic of micro-habitats, due to its heterogeneity and complexity, and thus is a major ecosystems for the development of microbial populations. This microflora, fungi represent a large part of the biomass, and are of great importance. Aspergillus and Penicillium are the most important genera of fungi related human activities and the exclusive study of these in the soil is unusual. This study evaluated the diversity of fungi Aspergillus and Penicillium isolated from soil of five distinct areas in the Iron Quadrangle, and evaluated its potential toxigenic and correlations with soil nutrients. Twenty soil samples were collected, and for each of these samples, serial dilutions were made in DG18 and DRBC means. The species of Penicillium and Aspergillus were identified by morphological characteristics. The characterization of the unidentified isolates was performed using microscopy and the colonies pictures, as well as phylogenetic study of some of them. The production ocrotoxina A (OTA), citrinin and aflatoxins B1, B2, G1 and G2 was assessed by Thin Layer Chromatography. Diversity, richness and composition of species, and their relationship with soil properties was verified. They were isolated in 1984 specimens belonging to 88 different morphotypes, 50 of these unidentified. An isolate of A. ochraceoroseus been identified and may be the only specimen found outside the Ivory Coast. Phylogenetic analysis of unidentified morphotypes was inconclusive, requiring more genes to confirm the species. All isolates of A. carbonarius produced OTA; any isolated from A. niger and A. niger aggregate OTA produced; all isolates of A. flavus and A. parasiticus produced the respective aflatoxins. All P. citrinum produced citrinin, together with Penicillium sp. 04, 19 and 27. Luminescent several unidentified substances were produced by unidentified morphotypes, which can help identify these and can be compounds of industrial interest. The areas were heterogeneous in diversity, richness and species composition. Grass and Eucalyptus were more representative in richness and abundance of species. Some soil attributes were highly associated with the occurrence and abundance of species of Aspergillus and Penicillium in soils. The species evaluated in this work, are possibly resistant Fe, Mn, Cu, Al and S and can presumably be used in bioremediation of contaminated sites. |
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Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero FerríferoDiversity, richness and composition of genres Aspergillus and Penicillium in soil of Iron QuadrangleFungos no soloSolos – NutrienteMicotoxinasSoil fungiSoil – NutrientMycotoxinsAspergillus sp.Penicillium sp.Microbiologia e Bioquímica do SoloBrazil has the largest global diversity index, however we have witnessed the high percentage of environmental devastation. Maintaining species in the laboratory, together with innovation, are the best alternative to maintai n that diversity. The soil is considered to be a mosaic of micro-habitats, due to its heterogeneity and complexity, and thus is a major ecosystems for the development of microbial populations. This microflora, fungi represent a large part of the biomass, and are of great importance. Aspergillus and Penicillium are the most important genera of fungi related human activities and the exclusive study of these in the soil is unusual. This study evaluated the diversity of fungi Aspergillus and Penicillium isolated from soil of five distinct areas in the Iron Quadrangle, and evaluated its potential toxigenic and correlations with soil nutrients. Twenty soil samples were collected, and for each of these samples, serial dilutions were made in DG18 and DRBC means. The species of Penicillium and Aspergillus were identified by morphological characteristics. The characterization of the unidentified isolates was performed using microscopy and the colonies pictures, as well as phylogenetic study of some of them. The production ocrotoxina A (OTA), citrinin and aflatoxins B1, B2, G1 and G2 was assessed by Thin Layer Chromatography. Diversity, richness and composition of species, and their relationship with soil properties was verified. They were isolated in 1984 specimens belonging to 88 different morphotypes, 50 of these unidentified. An isolate of A. ochraceoroseus been identified and may be the only specimen found outside the Ivory Coast. Phylogenetic analysis of unidentified morphotypes was inconclusive, requiring more genes to confirm the species. All isolates of A. carbonarius produced OTA; any isolated from A. niger and A. niger aggregate OTA produced; all isolates of A. flavus and A. parasiticus produced the respective aflatoxins. All P. citrinum produced citrinin, together with Penicillium sp. 04, 19 and 27. Luminescent several unidentified substances were produced by unidentified morphotypes, which can help identify these and can be compounds of industrial interest. The areas were heterogeneous in diversity, richness and species composition. Grass and Eucalyptus were more representative in richness and abundance of species. Some soil attributes were highly associated with the occurrence and abundance of species of Aspergillus and Penicillium in soils. The species evaluated in this work, are possibly resistant Fe, Mn, Cu, Al and S and can presumably be used in bioremediation of contaminated sites.Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)ValeO Brasil possui a maior diversidade global, entretanto tem testemunhado a percentuais elevados de devastação ambiental. A manutenção de espécies em laboratório, aliado a inovação, são a melhor alternativa para a manutenção dessa diversidade. O solo é considerado um mosaico de micro-habitats devido a sua heterogeneidade e complexidade e, dessa forma é um dos principais ecossistemas para o desenvolvimento de populações microbianas. Dessa microbiota, os fungos representam grande parte da biomassa, e são de grande importância. Aspergillus e Penicillium são os mais importantes gêneros de fungos ligados as atividades humanas e o estudo exclusivo destes no solo é pouco comum. Este estudo teve como objetivo avaliar a diversidade de fungos dos gêneros Aspergillus e Penicillium isolados do solo de cinco áreas no Quadrilátero Ferrífero, bem como avaliar seu potencial toxigênico e as correlações com os nutrientes do solo. Foram coletadas 20 amostras de solo, e para cada uma destas foram realizadas diluições seriadas nos meios DG18 e DRBC. As espécies dos gêneros Penicillium e Aspergillus foram identificadas através de características morfológicas. Foi realizada a caracterização dos isolados não identificados, utilizando fotografias das colônias e microscopia, assim como o estudo filogenético de alguns deles. A produção de ocrotoxina A (OTA), citrinina e aflatoxinas B1, B2, G1 e G2 foi avaliada por cromatografia de camada delgada. Foi verificada a diversidade, riqueza e composição das espécies e sua relação com os atributos do solo. Foram isolados 1984 espécimes pertencentes a 88 morfotipos distintos, sendo 50 destes não identificados. Um isolado de A. ochraceoroseus foi identificado e pode ser o único espécime encontrado fora da Costa do Marfim. A análise filogenética dos morfotipos não identificados foi inconclusiva, sendo necessários mais genes para confirmação das espécies. Todos isolados de A. carbonarius produziram OTA; nenhum isolado de A. niger e A. niger agregado produziram OTA; todos isolados de A. flavus e A. parasiticus produziram as respectivas aflatoxinas. Todos isolads de P. citrinum produziram citrinina, assim como os morfotipos Penicillium sp. 04, 19 e 27. Foi visualizado a produção de várias substâncias luminescentes não identificadas dos morfotipos não identificados, o que pode auxiliar na identificação desses morfotipos e caracterizarem compostos de interesse industrial. As áreas foram heterogêneas em diversidade, riqueza e composição de espécies. Capim e Eucalipto foram mais representativas em riqueza e abundância de espécies. Alguns atributos do solo foram altamente associados à ocorrência e abundância das espécies de Aspergillus e Penicillium nos solos. As espécies avaliadas nesse trabalho, possivelmente são resistentes a Fe, Mn, Cu, Al e S e presumivelmente podem ser utilizadas na biorremediação de sítios contaminados.Universidade Federal de LavrasPrograma de Pós-Graduação em Microbiologia AgrícolaUFLAbrasilDepartamento de BiologiaBatista, Luís RobertoSouza, Sara Maria Chalfoun deSouza, Sara Maria Chalfoun dePassamani, Fabiana Reins FrancaBatista, Cristina Ferreira Silva eCunha, Rodrigo Luz daPereira, Vanessa Maria2016-09-27T12:01:55Z2016-09-27T12:01:55Z2016-09-262016-09-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfPEREIRA, V. M. Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero. 2016. 222 p. Tese (Doutorado em Agroquímica)-Universidade Federal de Lavras, Lavras, 2016.http://repositorio.ufla.br/jspui/handle/1/11827porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2023-04-24T21:53:48Zoai:localhost:1/11827Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-04-24T21:53:48Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero Diversity, richness and composition of genres Aspergillus and Penicillium in soil of Iron Quadrangle |
title |
Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero |
spellingShingle |
Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero Pereira, Vanessa Maria Fungos no solo Solos – Nutriente Micotoxinas Soil fungi Soil – Nutrient Mycotoxins Aspergillus sp. Penicillium sp. Microbiologia e Bioquímica do Solo |
title_short |
Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero |
title_full |
Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero |
title_fullStr |
Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero |
title_full_unstemmed |
Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero |
title_sort |
Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero |
author |
Pereira, Vanessa Maria |
author_facet |
Pereira, Vanessa Maria |
author_role |
author |
dc.contributor.none.fl_str_mv |
Batista, Luís Roberto Souza, Sara Maria Chalfoun de Souza, Sara Maria Chalfoun de Passamani, Fabiana Reins Franca Batista, Cristina Ferreira Silva e Cunha, Rodrigo Luz da |
dc.contributor.author.fl_str_mv |
Pereira, Vanessa Maria |
dc.subject.por.fl_str_mv |
Fungos no solo Solos – Nutriente Micotoxinas Soil fungi Soil – Nutrient Mycotoxins Aspergillus sp. Penicillium sp. Microbiologia e Bioquímica do Solo |
topic |
Fungos no solo Solos – Nutriente Micotoxinas Soil fungi Soil – Nutrient Mycotoxins Aspergillus sp. Penicillium sp. Microbiologia e Bioquímica do Solo |
description |
Brazil has the largest global diversity index, however we have witnessed the high percentage of environmental devastation. Maintaining species in the laboratory, together with innovation, are the best alternative to maintai n that diversity. The soil is considered to be a mosaic of micro-habitats, due to its heterogeneity and complexity, and thus is a major ecosystems for the development of microbial populations. This microflora, fungi represent a large part of the biomass, and are of great importance. Aspergillus and Penicillium are the most important genera of fungi related human activities and the exclusive study of these in the soil is unusual. This study evaluated the diversity of fungi Aspergillus and Penicillium isolated from soil of five distinct areas in the Iron Quadrangle, and evaluated its potential toxigenic and correlations with soil nutrients. Twenty soil samples were collected, and for each of these samples, serial dilutions were made in DG18 and DRBC means. The species of Penicillium and Aspergillus were identified by morphological characteristics. The characterization of the unidentified isolates was performed using microscopy and the colonies pictures, as well as phylogenetic study of some of them. The production ocrotoxina A (OTA), citrinin and aflatoxins B1, B2, G1 and G2 was assessed by Thin Layer Chromatography. Diversity, richness and composition of species, and their relationship with soil properties was verified. They were isolated in 1984 specimens belonging to 88 different morphotypes, 50 of these unidentified. An isolate of A. ochraceoroseus been identified and may be the only specimen found outside the Ivory Coast. Phylogenetic analysis of unidentified morphotypes was inconclusive, requiring more genes to confirm the species. All isolates of A. carbonarius produced OTA; any isolated from A. niger and A. niger aggregate OTA produced; all isolates of A. flavus and A. parasiticus produced the respective aflatoxins. All P. citrinum produced citrinin, together with Penicillium sp. 04, 19 and 27. Luminescent several unidentified substances were produced by unidentified morphotypes, which can help identify these and can be compounds of industrial interest. The areas were heterogeneous in diversity, richness and species composition. Grass and Eucalyptus were more representative in richness and abundance of species. Some soil attributes were highly associated with the occurrence and abundance of species of Aspergillus and Penicillium in soils. The species evaluated in this work, are possibly resistant Fe, Mn, Cu, Al and S and can presumably be used in bioremediation of contaminated sites. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-27T12:01:55Z 2016-09-27T12:01:55Z 2016-09-26 2016-09-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PEREIRA, V. M. Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero. 2016. 222 p. Tese (Doutorado em Agroquímica)-Universidade Federal de Lavras, Lavras, 2016. http://repositorio.ufla.br/jspui/handle/1/11827 |
identifier_str_mv |
PEREIRA, V. M. Diversidade, riqueza e composição dos gêneros Aspergillus e Penicillium de solos do Quadrilátero Ferrífero. 2016. 222 p. Tese (Doutorado em Agroquímica)-Universidade Federal de Lavras, Lavras, 2016. |
url |
http://repositorio.ufla.br/jspui/handle/1/11827 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Microbiologia Agrícola UFLA brasil Departamento de Biologia |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Microbiologia Agrícola UFLA brasil Departamento de Biologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
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Universidade Federal de Lavras (UFLA) |
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UFLA |
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UFLA |
reponame_str |
Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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