Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.

Detalhes bibliográficos
Autor(a) principal: Santana, José Raniere Ferreira de
Data de Publicação: 2011
Outros Autores: Paiva, Renato, Souza, Ana Valéria de, Oliveira, Lenaldo Muniz de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000500008
http://repositorio.ufla.br/jspui/handle/1/7454
Resumo: The present work evaluated the effects of different types of culture flask seals and varying concentrations of sucrose and activated charcoal on the in vitro induction and growth of buds of Annona glabra L.; an edible fruit-producing species popularly known as "araticum". Nodal segments obtained from A. glabra plants maintained in green houses were surface sterilized and inoculated into a WPM culture medium solidified with 7 g L-1 of agar and supplemented with sucrose (0.00; 29.21; 58.63 and 116.84 mM), activated charcoal (0.0 and 2.0 g L-1), and 250 mg L-1 benomyl. In addition to the varying concentrations of sucrose and activated charcoal, we evaluated the efficiency of two types of test tube seals: PVC film, and cotton plugs. All possible combinations of caps and nutrient media were tested with 4 repetitions with 5 tubes each, evaluating the number of buds, the percentage of explant responses, the number of expanded leaves per bud, the length of the largest leaves, leaf abscission, and the length and dry weight of the buds. The type of seal influenced organogenesis in nodal segments of A. glabra, and no bud induction was observed in the absence of sucrose. The largest number of expanded leaves were obtained when 58.42 mM of sucrose was used in tubes sealed with cotton plugs, and leaf abscission was halved in the presence of activated charcoal. The greatest bud length and dry weight were obtained in tubes sealed with cotton plugs and in the presence of activated charcoal.
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spelling Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.Efeito do tipo de selamento do recipiente de cultura, diferentes concentrações de carvão ativado e sacarose na indução e crescimento de brotações in vitro de Annona glabra L.AnnonaceaeCotton plugsLeaf abscissionTampão de algodãoAbscisão foliarThe present work evaluated the effects of different types of culture flask seals and varying concentrations of sucrose and activated charcoal on the in vitro induction and growth of buds of Annona glabra L.; an edible fruit-producing species popularly known as "araticum". Nodal segments obtained from A. glabra plants maintained in green houses were surface sterilized and inoculated into a WPM culture medium solidified with 7 g L-1 of agar and supplemented with sucrose (0.00; 29.21; 58.63 and 116.84 mM), activated charcoal (0.0 and 2.0 g L-1), and 250 mg L-1 benomyl. In addition to the varying concentrations of sucrose and activated charcoal, we evaluated the efficiency of two types of test tube seals: PVC film, and cotton plugs. All possible combinations of caps and nutrient media were tested with 4 repetitions with 5 tubes each, evaluating the number of buds, the percentage of explant responses, the number of expanded leaves per bud, the length of the largest leaves, leaf abscission, and the length and dry weight of the buds. The type of seal influenced organogenesis in nodal segments of A. glabra, and no bud induction was observed in the absence of sucrose. The largest number of expanded leaves were obtained when 58.42 mM of sucrose was used in tubes sealed with cotton plugs, and leaf abscission was halved in the presence of activated charcoal. The greatest bud length and dry weight were obtained in tubes sealed with cotton plugs and in the presence of activated charcoal.Este trabalho foi realizado com o objetivo de avaliar o efeito do tipo de selamento do recipiente de cultura e diferentes concentrações de sacarose e carvão ativado na indução e crescimento de brotações in vitro de Annona glabra L.; espécie frutífera conhecida popularmente como araticum. Segmentos nodais obtidos de plantas de A. glabra mantidas em casa de vegetação, após o processo de assepsia, foram inoculados em meio de cultura WPM com 7 g L-1 de agar e sacarose (0,00; 29,21; 58,63 e 116,84 mM) combinado com carvão ativado (0,0 e 2,0 g L-1) e 250 mg L-1 de benomil. Avaliaram-se dois tipos de selamento nos tubos de ensaio: tampa com película de PVC e tampão de algodão. O experimento foi instalado em DIC em arranjo fatorial 4x2x2, com 4 repetições/5 tubos por parcela. Avaliou-se o número brotações, a porcentagem de explantes responsivos, o número de folhas expandidas por brotação, o comprimento da maior folha, a abscisão foliar, o comprimento e a matéria seca das brotações. O tipo de selamento influenciou a organogênese em segmentos nodais de A. glabra. Contudo, não houve indução de brotações na ausência de sacarose. O maior número de folhas expandidas foi obtido quando utilizou-se 58,42 mM de sacarose em tubos selados com tampão de algodão e a abscisão foliar foi reduzida à metade na presença do carvão ativado. Maiores comprimentos e maiores pesos da matéria seca das brotações foram obtidos em tubos selados com tampão de algodão, também na presença de carvão ativado.Editora da Universidade Federal de Lavras2011-10-012015-04-30T13:36:15Z2015-04-30T13:36:15Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000500008SANTANA, J. R. F. de et al. Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L. Ciência e Agrotecnologia, Lavras, v. 35, n. 5, p. 916-923, set./out. 2011.http://repositorio.ufla.br/jspui/handle/1/7454Ciência e Agrotecnologia v.35 n.5 2011reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLASantana, José Raniere Ferreira dePaiva, RenatoSouza, Ana Valéria deOliveira, Lenaldo Muniz deenginfo:eu-repo/semantics/openAccess2016-06-07T20:29:22Zoai:localhost:1/7454Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-06-07T20:29:22Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.
Efeito do tipo de selamento do recipiente de cultura, diferentes concentrações de carvão ativado e sacarose na indução e crescimento de brotações in vitro de Annona glabra L.
title Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.
spellingShingle Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.
Santana, José Raniere Ferreira de
Annonaceae
Cotton plugs
Leaf abscission
Tampão de algodão
Abscisão foliar
title_short Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.
title_full Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.
title_fullStr Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.
title_full_unstemmed Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.
title_sort Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L.
author Santana, José Raniere Ferreira de
author_facet Santana, José Raniere Ferreira de
Paiva, Renato
Souza, Ana Valéria de
Oliveira, Lenaldo Muniz de
author_role author
author2 Paiva, Renato
Souza, Ana Valéria de
Oliveira, Lenaldo Muniz de
author2_role author
author
author
dc.contributor.author.fl_str_mv Santana, José Raniere Ferreira de
Paiva, Renato
Souza, Ana Valéria de
Oliveira, Lenaldo Muniz de
dc.subject.por.fl_str_mv Annonaceae
Cotton plugs
Leaf abscission
Tampão de algodão
Abscisão foliar
topic Annonaceae
Cotton plugs
Leaf abscission
Tampão de algodão
Abscisão foliar
description The present work evaluated the effects of different types of culture flask seals and varying concentrations of sucrose and activated charcoal on the in vitro induction and growth of buds of Annona glabra L.; an edible fruit-producing species popularly known as "araticum". Nodal segments obtained from A. glabra plants maintained in green houses were surface sterilized and inoculated into a WPM culture medium solidified with 7 g L-1 of agar and supplemented with sucrose (0.00; 29.21; 58.63 and 116.84 mM), activated charcoal (0.0 and 2.0 g L-1), and 250 mg L-1 benomyl. In addition to the varying concentrations of sucrose and activated charcoal, we evaluated the efficiency of two types of test tube seals: PVC film, and cotton plugs. All possible combinations of caps and nutrient media were tested with 4 repetitions with 5 tubes each, evaluating the number of buds, the percentage of explant responses, the number of expanded leaves per bud, the length of the largest leaves, leaf abscission, and the length and dry weight of the buds. The type of seal influenced organogenesis in nodal segments of A. glabra, and no bud induction was observed in the absence of sucrose. The largest number of expanded leaves were obtained when 58.42 mM of sucrose was used in tubes sealed with cotton plugs, and leaf abscission was halved in the presence of activated charcoal. The greatest bud length and dry weight were obtained in tubes sealed with cotton plugs and in the presence of activated charcoal.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-01
2015-04-30T13:36:15Z
2015-04-30T13:36:15Z
2015-04-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000500008
SANTANA, J. R. F. de et al. Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L. Ciência e Agrotecnologia, Lavras, v. 35, n. 5, p. 916-923, set./out. 2011.
http://repositorio.ufla.br/jspui/handle/1/7454
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000500008
http://repositorio.ufla.br/jspui/handle/1/7454
identifier_str_mv SANTANA, J. R. F. de et al. Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L. Ciência e Agrotecnologia, Lavras, v. 35, n. 5, p. 916-923, set./out. 2011.
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
dc.source.none.fl_str_mv Ciência e Agrotecnologia v.35 n.5 2011
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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