Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/42120 |
Resumo: | The objetive of the currently study was to evaluate the effects of combination between ionophores and non-ionofores additives on performance, carcass traits and lipid turnover over time in feedlot of Nellore Angus young bulls. Seventy-two animals were utilized, with initial body weight 417.4 ± 41.0 kg, and randomly allotted in 18 pens, totalizing 4 animals per pen, in a completely randomized design. The three treatments consisted of supplementation of monensin (MON), monensin plus zinc bacitracin (MONZB) or monensin plus virginiamycin (MONVM). Monensin, zinc bacitracin and virginiamycin doses were 25.0, 8.75 and 25.0 mg/kg dry matter, respectively. The facilities and diet adaptation period lasted 27 d and the experimental period 82 d. The diet consisted in 15% of corn silage and 85% of concentrate. It was performed muscle biopsy from longissimus thoracis on d 7 of the experimental period to RT-qPCR analysis. In addition, blood samples were collected on d 7 and 43 of the experimental period to measured serum D-lactate concentration. At slaughter were collected muscle samples. There was no significant effect on overall performance, feed efficiency and most of carcass traits (P > 0.16). Animals fed MONVM presented lower average daily gain (ADG) in the beginning of the feedlot (P = 0.02). On the other hand, in the end of the feedlot the same treatment presented greater ADG (P = 0.08). Serum D-lactate concentration was similar among the three treatments and was not affected by time (P > 0.40). Animals fed MONVM had higher number of meals compared to animals fed MON (P = 0.03). On the other hand, animals fed MON had greater preferential intake of middle particle size (8 to 19 mm) than animals fed MONVM (P = 0.05). While animals fed MON, also refuse more the small particles (< 8 mm) than other treatments (P < 0.01). The expression of SREBF1, PPARG, ACACA and SCD1 was not influenced by the additives either by time (P > 0.20). However, the expression of LPL and CD36 increased over time (P = 0.02), while FASN expression decreased (P = 0.07). MONZB presented greater expression of PPARA on d 7 and lower expression at slaughter (P = 0.07). CPT2 expression was lower on d 7 for MONZB and higher for MONVM at slaughter (P = 0.07). Thus, the alone or combined use of the additives did not improved animal overall performance either carcass traits, suggesting that additives can be used alone or combined. In addition, the changes in gene expression was not enough to modify chemical composition of the meat. |
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Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophoresDesempenho e metabolismo lipídico em tourinhos nelore x angus alimentados com aditivos ionóforos e não-ionóforosBacitracina de zincoVirginiamicinaMonensinaTurnover lipídicoVirginiamicinaFeedlotLipid turnoverLipogenesisMonensinVirginiamycinZinc bacitracinZootecniaThe objetive of the currently study was to evaluate the effects of combination between ionophores and non-ionofores additives on performance, carcass traits and lipid turnover over time in feedlot of Nellore Angus young bulls. Seventy-two animals were utilized, with initial body weight 417.4 ± 41.0 kg, and randomly allotted in 18 pens, totalizing 4 animals per pen, in a completely randomized design. The three treatments consisted of supplementation of monensin (MON), monensin plus zinc bacitracin (MONZB) or monensin plus virginiamycin (MONVM). Monensin, zinc bacitracin and virginiamycin doses were 25.0, 8.75 and 25.0 mg/kg dry matter, respectively. The facilities and diet adaptation period lasted 27 d and the experimental period 82 d. The diet consisted in 15% of corn silage and 85% of concentrate. It was performed muscle biopsy from longissimus thoracis on d 7 of the experimental period to RT-qPCR analysis. In addition, blood samples were collected on d 7 and 43 of the experimental period to measured serum D-lactate concentration. At slaughter were collected muscle samples. There was no significant effect on overall performance, feed efficiency and most of carcass traits (P > 0.16). Animals fed MONVM presented lower average daily gain (ADG) in the beginning of the feedlot (P = 0.02). On the other hand, in the end of the feedlot the same treatment presented greater ADG (P = 0.08). Serum D-lactate concentration was similar among the three treatments and was not affected by time (P > 0.40). Animals fed MONVM had higher number of meals compared to animals fed MON (P = 0.03). On the other hand, animals fed MON had greater preferential intake of middle particle size (8 to 19 mm) than animals fed MONVM (P = 0.05). While animals fed MON, also refuse more the small particles (< 8 mm) than other treatments (P < 0.01). The expression of SREBF1, PPARG, ACACA and SCD1 was not influenced by the additives either by time (P > 0.20). However, the expression of LPL and CD36 increased over time (P = 0.02), while FASN expression decreased (P = 0.07). MONZB presented greater expression of PPARA on d 7 and lower expression at slaughter (P = 0.07). CPT2 expression was lower on d 7 for MONZB and higher for MONVM at slaughter (P = 0.07). Thus, the alone or combined use of the additives did not improved animal overall performance either carcass traits, suggesting that additives can be used alone or combined. In addition, the changes in gene expression was not enough to modify chemical composition of the meat.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)O objetivo do presente trabalho foi avaliar os efeitos da associação entre aditivos ionóforos e não-ionóforos sobre o desempenho, características de carcaça e turnover lipídico ao longo do tempo no confinamento de tourinhos F1 Nelore Angus. Foram utilizados 72 animais, com peso corporal inicial de 417,4 ± 41,0 kg, que foram aleatoriamente alocados em 18 baias, totalizando 4 animais por baia, em um delineamento inteiramente casualizado. Os três tratamentos consistiam no fornecimento de monensina (MON), monensina e bacitracina de zinco (MONBZ) ou monensina e virginiamicina (MONVM). As doses de monensina, bacitracina de zinco e virginiamicina foram 25,0; 8,75 e 25,0 mg/kg matéria seca, respectivamente. O período de adaptação dos animais à dieta e às instalações foi de 27 dias e o período experimental durou 82 dias. A dieta era composta por 15% de silagem de milho e 85% de concentrado. Foi realizada biópsia do músculo longissimus thoracis no dia 7 do período experimental para análise de RT-qPCR. Também foram coletadas amostras de sangue nos dias 7 e 43 do período experimental para mensuração da concentração sérica de D-lactato. No abate também foram coletadas amostras de músculo. Não houve efeito significativo no desempenho total, eficiência alimentar, composição química da carne e na maioria das características de carcaça (P > 0,16). Os animais do tratamento MONVM apresentaram menor ganho médio diário (GMD) no início do confinamento (P = 0,02), já final do confinamento, os mesmos animais tenderam a apresentar maior GMD (P = 0,08). A concentração de D-lactato foi semelhante entre os três tratamentos e não foi afetada pelo tempo (P > 0,40). Os animais alimentados com MONVM tiveram maior número de refeições comparados com os animais alimentados com MON (P = 0,03). Por outro lado, os animais do tratamento MON tiveram maior seleção a favor das partículas médias (8 a 19 mm) do que o tratamento MONVM (P = 0,05). Enquanto que o tratamento MON também recusou mais as partículas pequenas (< 8 mm) do que os demais tratamentos (P < 0,01). A expressão dos genes SREBF1, PPARG, ACACA e SCD1 não foi influenciada pelos aditivos nem pelo tempo (P > 0,20). Todavia, a expressão dos genes LPL e CD36 aumentaram com o tempo (P = 0,02), enquanto que do gene FASN diminuiu (P = 0,07). O tratamento MONBZ apresentou maior expressão do PPARA no dia 7 e menor expressão no momento do abate (P = 0,07), já a expressão do CPT2 foi menor no tempo 7 para o tratamento MONBZ e maior no momento do abate para MONVM (P = 0,07). Diante disso, conclui-se que o uso isolado ou combinado dos aditivos não melhorou o desempenho dos animais nem as características de carcaça sugerindo que o uso pode ser realizado na forma isolada ou associada. Além disso, as alterações na expressão dos genes não foram suficientes para modificar a composição química da carne.Universidade Federal de LavrasPrograma de Pós-Graduação em ZootecniaUFLAbrasilDepartamento de ZootecniaLadeira, Marcio MachadoCasagrande, Daniel RumeLadeira, Marcio MachadoBatista, Erick DarlissonDuarte, Márcio de SouzaOliveira Júnior, José Maria de2020-07-29T11:12:23Z2020-07-29T11:12:23Z2020-07-272020-03-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfOLIVEIRA JÚNIOR, J. M. de O. Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores. 2020. 62 p. Dissertação (Mestrado em Zootecnia) – Universidade Federal de Lavras, Lavras, 2020.http://repositorio.ufla.br/jspui/handle/1/42120enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2023-05-31T13:55:45Zoai:localhost:1/42120Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-31T13:55:45Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores Desempenho e metabolismo lipídico em tourinhos nelore x angus alimentados com aditivos ionóforos e não-ionóforos |
title |
Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores |
spellingShingle |
Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores Oliveira Júnior, José Maria de Bacitracina de zinco Virginiamicina Monensina Turnover lipídico Virginiamicina Feedlot Lipid turnover Lipogenesis Monensin Virginiamycin Zinc bacitracin Zootecnia |
title_short |
Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores |
title_full |
Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores |
title_fullStr |
Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores |
title_full_unstemmed |
Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores |
title_sort |
Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores |
author |
Oliveira Júnior, José Maria de |
author_facet |
Oliveira Júnior, José Maria de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Ladeira, Marcio Machado Casagrande, Daniel Rume Ladeira, Marcio Machado Batista, Erick Darlisson Duarte, Márcio de Souza |
dc.contributor.author.fl_str_mv |
Oliveira Júnior, José Maria de |
dc.subject.por.fl_str_mv |
Bacitracina de zinco Virginiamicina Monensina Turnover lipídico Virginiamicina Feedlot Lipid turnover Lipogenesis Monensin Virginiamycin Zinc bacitracin Zootecnia |
topic |
Bacitracina de zinco Virginiamicina Monensina Turnover lipídico Virginiamicina Feedlot Lipid turnover Lipogenesis Monensin Virginiamycin Zinc bacitracin Zootecnia |
description |
The objetive of the currently study was to evaluate the effects of combination between ionophores and non-ionofores additives on performance, carcass traits and lipid turnover over time in feedlot of Nellore Angus young bulls. Seventy-two animals were utilized, with initial body weight 417.4 ± 41.0 kg, and randomly allotted in 18 pens, totalizing 4 animals per pen, in a completely randomized design. The three treatments consisted of supplementation of monensin (MON), monensin plus zinc bacitracin (MONZB) or monensin plus virginiamycin (MONVM). Monensin, zinc bacitracin and virginiamycin doses were 25.0, 8.75 and 25.0 mg/kg dry matter, respectively. The facilities and diet adaptation period lasted 27 d and the experimental period 82 d. The diet consisted in 15% of corn silage and 85% of concentrate. It was performed muscle biopsy from longissimus thoracis on d 7 of the experimental period to RT-qPCR analysis. In addition, blood samples were collected on d 7 and 43 of the experimental period to measured serum D-lactate concentration. At slaughter were collected muscle samples. There was no significant effect on overall performance, feed efficiency and most of carcass traits (P > 0.16). Animals fed MONVM presented lower average daily gain (ADG) in the beginning of the feedlot (P = 0.02). On the other hand, in the end of the feedlot the same treatment presented greater ADG (P = 0.08). Serum D-lactate concentration was similar among the three treatments and was not affected by time (P > 0.40). Animals fed MONVM had higher number of meals compared to animals fed MON (P = 0.03). On the other hand, animals fed MON had greater preferential intake of middle particle size (8 to 19 mm) than animals fed MONVM (P = 0.05). While animals fed MON, also refuse more the small particles (< 8 mm) than other treatments (P < 0.01). The expression of SREBF1, PPARG, ACACA and SCD1 was not influenced by the additives either by time (P > 0.20). However, the expression of LPL and CD36 increased over time (P = 0.02), while FASN expression decreased (P = 0.07). MONZB presented greater expression of PPARA on d 7 and lower expression at slaughter (P = 0.07). CPT2 expression was lower on d 7 for MONZB and higher for MONVM at slaughter (P = 0.07). Thus, the alone or combined use of the additives did not improved animal overall performance either carcass traits, suggesting that additives can be used alone or combined. In addition, the changes in gene expression was not enough to modify chemical composition of the meat. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07-29T11:12:23Z 2020-07-29T11:12:23Z 2020-07-27 2020-03-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
OLIVEIRA JÚNIOR, J. M. de O. Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores. 2020. 62 p. Dissertação (Mestrado em Zootecnia) – Universidade Federal de Lavras, Lavras, 2020. http://repositorio.ufla.br/jspui/handle/1/42120 |
identifier_str_mv |
OLIVEIRA JÚNIOR, J. M. de O. Performance and lipid metabolism in Nellore x Angus young bulls fed ionophores and non-ionophores. 2020. 62 p. Dissertação (Mestrado em Zootecnia) – Universidade Federal de Lavras, Lavras, 2020. |
url |
http://repositorio.ufla.br/jspui/handle/1/42120 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Zootecnia UFLA brasil Departamento de Zootecnia |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Zootecnia UFLA brasil Departamento de Zootecnia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1815439190197796864 |