Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/41005 |
Resumo: | Background: the search for new inhibitors of snake venom toxins is essential to complement or even replace traditional antivenom therapy, especially in relation to compounds that neutralize the local effects of envenomations. Besides their possible use as alternative to traditional antivenom therapy, some plant species possess bioactive secondary metabolites including essential oils, which can be extracted from weeds that are considered substantial problems for agriculture, such as Hedychium coronarium. Methods: the essential oils of leaves and rhizomes from H. coronarium were extracted by hydrodistillation, and their potential inhibitory effects on the coagulant and fibrinogenolytic activities induced by the venoms of Lachesis muta,Bothrops atrox and Bothrops moojeniwere analyzed. Citrated human plasma was used to evaluate the clotting time whereas changes in fibrinogen molecules were visualized by electrophoresis in polyacrylamide gel. The experimental design used for testing coagulation inhibition was randomized in a 3 × 2 factorial arrangement (concentration × essential oils), with three replications. The essential oils were compared since they were extracted from different organs of the same botanical species, H. coronarium. Results: the results suggest that the oils interact with venom proteases and plasma constituents, since all oils evaluated, when previously incubated with venoms, were able to inhibit the clotting effect, with less inhibition when oils and plasma were preincubated prior to the addition of venoms. Conclusions: thus, after extensive characterization of their pharmacological and toxicological effects, the essential oils can be used as an alternative to complement serum therapy, especially considering that these plant metabolites generally do not require specific formulations and may be used topically immediately after extraction. |
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Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venomsVolatile oilsWeedsNatural inhibitorsAntivenom therapyBackground: the search for new inhibitors of snake venom toxins is essential to complement or even replace traditional antivenom therapy, especially in relation to compounds that neutralize the local effects of envenomations. Besides their possible use as alternative to traditional antivenom therapy, some plant species possess bioactive secondary metabolites including essential oils, which can be extracted from weeds that are considered substantial problems for agriculture, such as Hedychium coronarium. Methods: the essential oils of leaves and rhizomes from H. coronarium were extracted by hydrodistillation, and their potential inhibitory effects on the coagulant and fibrinogenolytic activities induced by the venoms of Lachesis muta,Bothrops atrox and Bothrops moojeniwere analyzed. Citrated human plasma was used to evaluate the clotting time whereas changes in fibrinogen molecules were visualized by electrophoresis in polyacrylamide gel. The experimental design used for testing coagulation inhibition was randomized in a 3 × 2 factorial arrangement (concentration × essential oils), with three replications. The essential oils were compared since they were extracted from different organs of the same botanical species, H. coronarium. Results: the results suggest that the oils interact with venom proteases and plasma constituents, since all oils evaluated, when previously incubated with venoms, were able to inhibit the clotting effect, with less inhibition when oils and plasma were preincubated prior to the addition of venoms. Conclusions: thus, after extensive characterization of their pharmacological and toxicological effects, the essential oils can be used as an alternative to complement serum therapy, especially considering that these plant metabolites generally do not require specific formulations and may be used topically immediately after extraction.Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) - Câmpus de Botucatu2020-05-18T04:58:42Z2020-05-18T04:58:42Z2014-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMIRANDA, C. A. S. F. et al. Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms. Journal of Venomous Animals and Toxins including Tropical Diseases, Botucatu, v. 20, n. 39, p. 1-3, Oct. 2014. DOI: 10.1186/1678-9199-20-39.http://repositorio.ufla.br/jspui/handle/1/41005Journal of Venomous Animals and Toxins including Tropical Diseasesreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessMiranda, Cíntia A. S. F.Cardoso, Maria G.Mansanares, Mariana E.Gomes, Marcos S.Marcussi, Silvanaeng2020-05-18T04:58:42Zoai:localhost:1/41005Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-05-18T04:58:42Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms |
title |
Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms |
spellingShingle |
Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms Miranda, Cíntia A. S. F. Volatile oils Weeds Natural inhibitors Antivenom therapy |
title_short |
Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms |
title_full |
Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms |
title_fullStr |
Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms |
title_full_unstemmed |
Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms |
title_sort |
Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms |
author |
Miranda, Cíntia A. S. F. |
author_facet |
Miranda, Cíntia A. S. F. Cardoso, Maria G. Mansanares, Mariana E. Gomes, Marcos S. Marcussi, Silvana |
author_role |
author |
author2 |
Cardoso, Maria G. Mansanares, Mariana E. Gomes, Marcos S. Marcussi, Silvana |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Miranda, Cíntia A. S. F. Cardoso, Maria G. Mansanares, Mariana E. Gomes, Marcos S. Marcussi, Silvana |
dc.subject.por.fl_str_mv |
Volatile oils Weeds Natural inhibitors Antivenom therapy |
topic |
Volatile oils Weeds Natural inhibitors Antivenom therapy |
description |
Background: the search for new inhibitors of snake venom toxins is essential to complement or even replace traditional antivenom therapy, especially in relation to compounds that neutralize the local effects of envenomations. Besides their possible use as alternative to traditional antivenom therapy, some plant species possess bioactive secondary metabolites including essential oils, which can be extracted from weeds that are considered substantial problems for agriculture, such as Hedychium coronarium. Methods: the essential oils of leaves and rhizomes from H. coronarium were extracted by hydrodistillation, and their potential inhibitory effects on the coagulant and fibrinogenolytic activities induced by the venoms of Lachesis muta,Bothrops atrox and Bothrops moojeniwere analyzed. Citrated human plasma was used to evaluate the clotting time whereas changes in fibrinogen molecules were visualized by electrophoresis in polyacrylamide gel. The experimental design used for testing coagulation inhibition was randomized in a 3 × 2 factorial arrangement (concentration × essential oils), with three replications. The essential oils were compared since they were extracted from different organs of the same botanical species, H. coronarium. Results: the results suggest that the oils interact with venom proteases and plasma constituents, since all oils evaluated, when previously incubated with venoms, were able to inhibit the clotting effect, with less inhibition when oils and plasma were preincubated prior to the addition of venoms. Conclusions: thus, after extensive characterization of their pharmacological and toxicological effects, the essential oils can be used as an alternative to complement serum therapy, especially considering that these plant metabolites generally do not require specific formulations and may be used topically immediately after extraction. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10 2020-05-18T04:58:42Z 2020-05-18T04:58:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MIRANDA, C. A. S. F. et al. Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms. Journal of Venomous Animals and Toxins including Tropical Diseases, Botucatu, v. 20, n. 39, p. 1-3, Oct. 2014. DOI: 10.1186/1678-9199-20-39. http://repositorio.ufla.br/jspui/handle/1/41005 |
identifier_str_mv |
MIRANDA, C. A. S. F. et al. Preliminary assessment of Hedychium coronarium essential oil on fibrinogenolytic and coagulant activity induced by Bothrops and Lachesis snake venoms. Journal of Venomous Animals and Toxins including Tropical Diseases, Botucatu, v. 20, n. 39, p. 1-3, Oct. 2014. DOI: 10.1186/1678-9199-20-39. |
url |
http://repositorio.ufla.br/jspui/handle/1/41005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) - Câmpus de Botucatu |
publisher.none.fl_str_mv |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) - Câmpus de Botucatu |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1815439268124819456 |