The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem

Detalhes bibliográficos
Autor(a) principal: Balbi, Rodrigo Vieira
Data de Publicação: 2019
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/38266
Resumo: Pear cultivation in the tropics currently requires the complex use of Pyrus calleryana or Chaenomeles sinensis rootstocks with Cydonia oblonga interstocks. However, there is a disagreement in the literature regarding the tissues that are responsible for regeneration during grafting, especially during intergeneric grafting. The objective of the present study is to quantify the different proportions of tissues in rootstock-interstock combinations established to obtain saplings. Packham's Triumph pear (crown) was double grafted by cleft grafting onto interstocks of C. oblonga (Adams, BA-29, EMA, EMC and Sydo cultivars), and these combinations were subsequently grafted onto C. sinensis or P. calleryana rootstocks. The sprouting percentage was evaluated 150 days after sprouting. Subsequently, the anatomy of the connections between the crown and interstock and the interstock and rootstock was examined. The following characteristics were evaluated: xylem parenchyma ray area of each grafting component (scion, interstock and rootstock); total connection area; connection areas between xylem-xylem, phloem-phloem, phloem-cortex, and cortex-cortex; percentage of xylem, phloem, and cortex for all materials; rootstock area; interstock area; scion area; rootstock/interstock connection area; interstock/scion connection area; and areas with no connection or with necrosis. The data were subjected to analysis of variance and the ScottKnott test (P ≤ 0.05), and correlations between tissue percentages and connection areas were calculated and considered significant when R 2 ≥ 0.6. Only the tissues composing the cortex and phloem were positively correlated with the connection area. The P. calleryana rootstock has greater compatibility with the interstock due to its greater proportion of cortex. The xylem parenchyma rays do not significantly influence cell regeneration or connection. Therefore, woody species that contain more cortex and phloem in the rootstock and/or scion will have greater potential for grafting success.
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spelling The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloemCortex e floema determinam o processo de regeneração e conexão celular na enxertia entre pereiras e marmeleirosEnxertiaGraftingAnatomical compatibilityCellular connectionParenchymaWood anatomyFitotecniaPear cultivation in the tropics currently requires the complex use of Pyrus calleryana or Chaenomeles sinensis rootstocks with Cydonia oblonga interstocks. However, there is a disagreement in the literature regarding the tissues that are responsible for regeneration during grafting, especially during intergeneric grafting. The objective of the present study is to quantify the different proportions of tissues in rootstock-interstock combinations established to obtain saplings. Packham's Triumph pear (crown) was double grafted by cleft grafting onto interstocks of C. oblonga (Adams, BA-29, EMA, EMC and Sydo cultivars), and these combinations were subsequently grafted onto C. sinensis or P. calleryana rootstocks. The sprouting percentage was evaluated 150 days after sprouting. Subsequently, the anatomy of the connections between the crown and interstock and the interstock and rootstock was examined. The following characteristics were evaluated: xylem parenchyma ray area of each grafting component (scion, interstock and rootstock); total connection area; connection areas between xylem-xylem, phloem-phloem, phloem-cortex, and cortex-cortex; percentage of xylem, phloem, and cortex for all materials; rootstock area; interstock area; scion area; rootstock/interstock connection area; interstock/scion connection area; and areas with no connection or with necrosis. The data were subjected to analysis of variance and the ScottKnott test (P ≤ 0.05), and correlations between tissue percentages and connection areas were calculated and considered significant when R 2 ≥ 0.6. Only the tissues composing the cortex and phloem were positively correlated with the connection area. The P. calleryana rootstock has greater compatibility with the interstock due to its greater proportion of cortex. The xylem parenchyma rays do not significantly influence cell regeneration or connection. Therefore, woody species that contain more cortex and phloem in the rootstock and/or scion will have greater potential for grafting success.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)O porta-enxerto Pyrus calleryana possui restrições para as pereiras em virtude do alto vigor que proporciona à cultivar copa. A opção seria o marmeleiuro (Cydonia oblonga) como portaenxerto, porém não tolera solos ácidos. Outro possível porta-enxerto seria o marmeleiro da espécie Chaenomeles sinensis, porém, ainda, não apresenta compatibilidade com as pereiras. A alternativa seria o uso dos porta-enxertos Pyrus calleryana e Chaenomeles sinensis com interenxertos inter-Cydonia oblonga. No caso da enxertia, a literatura diverge quanto aos tecidos são responsáveis pela regeneração, o que precisa ser investigado com enxertias intergenéricas. O presente trabalho teve como objetivo quantificar as diferentes proporções dos tecidos, em combinações de porta-enxertos e interenxertos, no processo de obtenção de mudas da pereira. Em junho, realizou-se uma enxertia de mesa por garfagem da pereira Packham‟s triumph, nos interenxertos do gênero C. oblonga (cultivares Adams, BA-29, MA, MC e Sydom), para, então, garfos e interenxertos serem enxertados nos porta-enxertos C. sinensis e P. calleryana. Aos 150 dias após a realização da enxertia, foi avaliada a porcentagem de garfos brotados. Posteriormente, foi realizado o estudo da anatomia. O delineamento utilizado foi inteiramente casualizado, em fatorial 2 x 5, sendo o primeiro fator os dois porta-enxertos e o segundo fator, os cinco interenxertos do gênero Cydonia com três repetições de uma planta. Foram avaliadas uma lâmina, três cortes e cinco cortes, para cada repetição e realizada a média destes dados para cada repetição. As seguintes características foram avaliadas: as áreas dos raios parenquimáticos do xilema de cada componente de enxertia (enxerto, inter-enxerto e porta-enxerto); a área total da conexão, a área das conexões entre: xilema-xilema, floema-floema, floema-córtex e córtex-córtex; calcularam-se, também, as porcentagens do xilema, floema e córtex para todos os materiais; área do porta-enxerto; área do inter-enxerto; área do enxerto; área de conexão entre porta-enxerto/inter-enxerto; área de conexão entre interenxerto/enxerto e, por fim, áreas com ausência de conexão ou necrosadas. O índice de correlação foi calculado entre o percentual de tecidos e a área de conexão, e os dados foram considerados com correlação significativa, quando o R 2 apresentou valores ≥ 0,60. Os tecidos que compõem o córtex e o floema são os responsáveis diretos pela regeneração e conexão celular entre os componentes envolvidos no processo de enxertia. O xilema apresenta uma contribuição pouco significativa durante essa etapa da enxertia. O porta-enxerto Pyrus calleryana apresenta uma compatibilidade maior com os interenxertos da espécie Cydonia oblonga e com a copa „Packham‟s triumph‟. A superioridade do portaenxerto Pyrus calleryana está relacionada à maior proporção de córtex, o que favorece a regeneração e a conexão celular. Cultivares de materiais que contenham mais córtex e floema, no porta-enxerto ou enxerto, possuirão maior potencial para o sucesso no processo de enxertia. Os tecidos que compõem o córtex e o floema são responsáveis pela regeneração e conexão celular entre os componentes envolvidos no processo de enxertia. O porta-enxerto Pyrus calleryana apresenta compatibilidade maior com os interenxertos pela maior proporção de córtex. Espécies que contenham mais córtex e floema, no porta-enxerto ou enxerto, possuirão maior potencial para o sucesso no processo de enxertia.Universidade Federal de LavrasPrograma de Pós-Graduação em Agronomia/FitotecniaUFLAbrasilDepartamento de AgriculturaPio, RafaelAlvarenga, Ângelo AlbéricoPio, Leila Aparecida SallesPires, Marines FerreiraPereira , Fabrício JoséBalbi, Rodrigo Vieira2019-12-17T17:44:38Z2019-12-17T17:44:38Z2019-12-172019-10-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfBALBI, R. V. The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem. 2019. 52 p. Tese (Doutorado em Agronomia/Fitotecnia) – Universidade Federal de Lavras, Lavras, 2019.http://repositorio.ufla.br/jspui/handle/1/38266porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2019-12-17T17:45:41Zoai:localhost:1/38266Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-12-17T17:45:41Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem
Cortex e floema determinam o processo de regeneração e conexão celular na enxertia entre pereiras e marmeleiros
title The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem
spellingShingle The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem
Balbi, Rodrigo Vieira
Enxertia
Grafting
Anatomical compatibility
Cellular connection
Parenchyma
Wood anatomy
Fitotecnia
title_short The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem
title_full The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem
title_fullStr The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem
title_full_unstemmed The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem
title_sort The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem
author Balbi, Rodrigo Vieira
author_facet Balbi, Rodrigo Vieira
author_role author
dc.contributor.none.fl_str_mv Pio, Rafael
Alvarenga, Ângelo Albérico
Pio, Leila Aparecida Salles
Pires, Marines Ferreira
Pereira , Fabrício José
dc.contributor.author.fl_str_mv Balbi, Rodrigo Vieira
dc.subject.por.fl_str_mv Enxertia
Grafting
Anatomical compatibility
Cellular connection
Parenchyma
Wood anatomy
Fitotecnia
topic Enxertia
Grafting
Anatomical compatibility
Cellular connection
Parenchyma
Wood anatomy
Fitotecnia
description Pear cultivation in the tropics currently requires the complex use of Pyrus calleryana or Chaenomeles sinensis rootstocks with Cydonia oblonga interstocks. However, there is a disagreement in the literature regarding the tissues that are responsible for regeneration during grafting, especially during intergeneric grafting. The objective of the present study is to quantify the different proportions of tissues in rootstock-interstock combinations established to obtain saplings. Packham's Triumph pear (crown) was double grafted by cleft grafting onto interstocks of C. oblonga (Adams, BA-29, EMA, EMC and Sydo cultivars), and these combinations were subsequently grafted onto C. sinensis or P. calleryana rootstocks. The sprouting percentage was evaluated 150 days after sprouting. Subsequently, the anatomy of the connections between the crown and interstock and the interstock and rootstock was examined. The following characteristics were evaluated: xylem parenchyma ray area of each grafting component (scion, interstock and rootstock); total connection area; connection areas between xylem-xylem, phloem-phloem, phloem-cortex, and cortex-cortex; percentage of xylem, phloem, and cortex for all materials; rootstock area; interstock area; scion area; rootstock/interstock connection area; interstock/scion connection area; and areas with no connection or with necrosis. The data were subjected to analysis of variance and the ScottKnott test (P ≤ 0.05), and correlations between tissue percentages and connection areas were calculated and considered significant when R 2 ≥ 0.6. Only the tissues composing the cortex and phloem were positively correlated with the connection area. The P. calleryana rootstock has greater compatibility with the interstock due to its greater proportion of cortex. The xylem parenchyma rays do not significantly influence cell regeneration or connection. Therefore, woody species that contain more cortex and phloem in the rootstock and/or scion will have greater potential for grafting success.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-17T17:44:38Z
2019-12-17T17:44:38Z
2019-12-17
2019-10-19
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv BALBI, R. V. The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem. 2019. 52 p. Tese (Doutorado em Agronomia/Fitotecnia) – Universidade Federal de Lavras, Lavras, 2019.
http://repositorio.ufla.br/jspui/handle/1/38266
identifier_str_mv BALBI, R. V. The cell regeneration and conection on grafting between pear and quince trees is defined by cortex and phloem. 2019. 52 p. Tese (Doutorado em Agronomia/Fitotecnia) – Universidade Federal de Lavras, Lavras, 2019.
url http://repositorio.ufla.br/jspui/handle/1/38266
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Agronomia/Fitotecnia
UFLA
brasil
Departamento de Agricultura
publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Agronomia/Fitotecnia
UFLA
brasil
Departamento de Agricultura
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815439026459508736

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