The wild sugarcane and sorghum kinomes: insights into expansion, diversification, and expression patterns

• Autor(a) principal: Aono, Alexandre Hild
• Data de Publicação: 2021
• Outros Autores: Pimenta, Ricardo José Gonzaga, Garcia, Ana Letycia Basso, Correr, Fernando Henrique, Hosaka, Guilherme Kenichi, Carrasco, Marishani Marin, Cardoso-Silva, Cláudio Benício, Mancini, Melina Cristina, Sforça, Danilo Augusto, Santos, Lucas Borges dos, Nagai, James Shiniti, Pinto, Luciana Rossini, Landell, Marcos Guimarães de Andrade, Carneiro, Monalisa Sampaio, Balsalobre, Thiago Willian, Quiles, Marcos Gonçalves, Pereira, Welison Andrade, Margarido, Gabriel Rodrigues Alves, Souza, Anete Pereira de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFLA
• Texto Completo: http://repositorio.ufla.br/jspui/handle/1/48691
• topic: Coexpression networks; Kinase gene family; Phylogenetic analyses; RNA-seq; Saccharum spontaneum; Sorghum bicolor; Redes de coexpressão; Família de genes quinase; Análises filogenéticas; Sorgo bicolor

The protein kinase (PK) superfamily is one of the largest superfamilies in plants and the core regulator of cellular signaling. Despite this substantial importance, the kinomes of sugarcane and sorghum have not been profiled. Here, we identified and profiled the complete kinomes of the polyploid Saccharum spontaneum (Ssp) and Sorghum bicolor (Sbi), a close diploid relative. The Sbi kinome was composed of 1,210 PKs; for Ssp, we identified 2,919 PKs when disregarding duplications and allelic copies, and these were related to 1,345 representative gene models. The Ssp and Sbi PKs were grouped into 20 groups and 120 subfamilies and exhibited high compositional similarities and evolutionary divergences. By utilizing the collinearity between the species, this study offers insights into Sbi and Ssp speciation, PK differentiation and selection. We assessed the PK subfamily expression profiles via RNA-Seq and identified significant similarities between Sbi and Ssp. Moreover, coexpression networks allowed inference of a core structure of kinase interactions with specific key elements. This study provides the first categorization of the allelic specificity of a kinome and offers a wide reservoir of molecular and genetic information, thereby enhancing the understanding of Sbi and Ssp PK evolutionary history