Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying

Detalhes bibliográficos
Autor(a) principal: Guimarães, Cristiane Carvalho
Data de Publicação: 2017
Outros Autores: Oliveira, Julio Maia, Faria, José Marcio Rocha, Silva, Edvaldo Aparecido Amaral da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/34012
Resumo: The aim of this study was to evaluate DNA integrity and to identify ultrastructural changes in the primary root of Peltophorum dubium seeds subjected to drying, since these changes may reflect the germination capacity. Seeds were germinated and were dehydrated when reached 1 mm in length of the primary root, until reaching different moisture contents, being then rehydrated and evaluated in relation to survival. After this procedure, different points of interest (control, 20% and 10% moisture content) were selected to evaluate DNA and cell ultrastructure integrity in order to detect possible cellular changes in primary roots after dehydration. When dried down to 32% moisture, there was no influence on the growth resumption of the germinated seeds, but when they were dehydrated to 20% moisture, the survival was reduced to 67%, reaching nullity in the survival with drying to 8% moisture. By the evaluation of ultrastructural changes in root cells, it was observed that drying to approximately 20% moisture content marked the onset of damages by desiccation, both physiologically and ultrastructurally. Drying to levels close to 10% moisture content was not only responsible for total disorganization and rupture of membranes, but also promoted intracellular collapse. Loss of genomic DNA integrity was observed during drying.
id UFLA_a4a57c5848b8c6a773a88f9cea9fb377
oai_identifier_str oai:localhost:1/34012
network_acronym_str UFLA
network_name_str Repositório Institucional da UFLA
repository_id_str
spelling Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to dryingMudanças ultra-estruturais e integridade do DNA genômico em sementes germinadas de peltophorum dubium submetidas à secagemDesiccation toleranceCellular alterationsDNA damageTolerância à dessecaçãoDanos ao DNAThe aim of this study was to evaluate DNA integrity and to identify ultrastructural changes in the primary root of Peltophorum dubium seeds subjected to drying, since these changes may reflect the germination capacity. Seeds were germinated and were dehydrated when reached 1 mm in length of the primary root, until reaching different moisture contents, being then rehydrated and evaluated in relation to survival. After this procedure, different points of interest (control, 20% and 10% moisture content) were selected to evaluate DNA and cell ultrastructure integrity in order to detect possible cellular changes in primary roots after dehydration. When dried down to 32% moisture, there was no influence on the growth resumption of the germinated seeds, but when they were dehydrated to 20% moisture, the survival was reduced to 67%, reaching nullity in the survival with drying to 8% moisture. By the evaluation of ultrastructural changes in root cells, it was observed that drying to approximately 20% moisture content marked the onset of damages by desiccation, both physiologically and ultrastructurally. Drying to levels close to 10% moisture content was not only responsible for total disorganization and rupture of membranes, but also promoted intracellular collapse. Loss of genomic DNA integrity was observed during drying.Objetivou-se com esse trabalho avaliar a integridade do DNA e identificar mudanças ultra-estruturais em raiz primária de sementes de Peltophorum dubium submetidas a secagem. Para tanto, sementes foram posta a germinar e ao atingirem 1 mm de comprimento da raiz primária, foram desidratadas, até atingirem diferentes graus de umidade, sendo em seguida reidratadas e avaliadas quanto à sobrevivência. Após esse procedimento foram selecionados diferentes pontos de interesse (controle, 20% e 10% de umidade) para avaliação da integridade do DNA e da ultraestrutura celular no intuito de detectar possíveis mudanças celulares nas raízes primárias após desidratação. Quando secas até 32% de umidade não houve influência na retomada do crescimento das sementes germinadas, mas ao serem desidratadas a 20% de umidade, a sobrevivência foi reduzida a 67% e a secagem a 8% de umidade concorreu para nulidade na sobrevivência. Pela avaliação de mudanças ultraestruturais em células da raiz observou-se que secagem a aproximadamente 20% de umidade marcou o início dos danos por dessecação, tanto fisiológica quanto ultraestruturalmente. Secagem a níveis próximos a 10% de umidade concorreu não somente para total desconfiguração e ruptura das membranas como também promoveu colapso intracelular. Foi observada perda da integridade do DNA genômico no decorrer da secagem.Sociedade de Investigações Florestais2019-05-07T11:21:29Z2019-05-07T11:21:29Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGUIMARÃES, C. C. et al. Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying. Revista Árvore, Viçosa, MG, v. 41, n. 4, 2017.http://repositorio.ufla.br/jspui/handle/1/34012Revista Árvorereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessGuimarães, Cristiane CarvalhoOliveira, Julio MaiaFaria, José Marcio RochaSilva, Edvaldo Aparecido Amaral daeng2019-05-07T11:21:30Zoai:localhost:1/34012Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-05-07T11:21:30Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying
Mudanças ultra-estruturais e integridade do DNA genômico em sementes germinadas de peltophorum dubium submetidas à secagem
title Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying
spellingShingle Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying
Guimarães, Cristiane Carvalho
Desiccation tolerance
Cellular alterations
DNA damage
Tolerância à dessecação
Danos ao DNA
title_short Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying
title_full Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying
title_fullStr Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying
title_full_unstemmed Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying
title_sort Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying
author Guimarães, Cristiane Carvalho
author_facet Guimarães, Cristiane Carvalho
Oliveira, Julio Maia
Faria, José Marcio Rocha
Silva, Edvaldo Aparecido Amaral da
author_role author
author2 Oliveira, Julio Maia
Faria, José Marcio Rocha
Silva, Edvaldo Aparecido Amaral da
author2_role author
author
author
dc.contributor.author.fl_str_mv Guimarães, Cristiane Carvalho
Oliveira, Julio Maia
Faria, José Marcio Rocha
Silva, Edvaldo Aparecido Amaral da
dc.subject.por.fl_str_mv Desiccation tolerance
Cellular alterations
DNA damage
Tolerância à dessecação
Danos ao DNA
topic Desiccation tolerance
Cellular alterations
DNA damage
Tolerância à dessecação
Danos ao DNA
description The aim of this study was to evaluate DNA integrity and to identify ultrastructural changes in the primary root of Peltophorum dubium seeds subjected to drying, since these changes may reflect the germination capacity. Seeds were germinated and were dehydrated when reached 1 mm in length of the primary root, until reaching different moisture contents, being then rehydrated and evaluated in relation to survival. After this procedure, different points of interest (control, 20% and 10% moisture content) were selected to evaluate DNA and cell ultrastructure integrity in order to detect possible cellular changes in primary roots after dehydration. When dried down to 32% moisture, there was no influence on the growth resumption of the germinated seeds, but when they were dehydrated to 20% moisture, the survival was reduced to 67%, reaching nullity in the survival with drying to 8% moisture. By the evaluation of ultrastructural changes in root cells, it was observed that drying to approximately 20% moisture content marked the onset of damages by desiccation, both physiologically and ultrastructurally. Drying to levels close to 10% moisture content was not only responsible for total disorganization and rupture of membranes, but also promoted intracellular collapse. Loss of genomic DNA integrity was observed during drying.
publishDate 2017
dc.date.none.fl_str_mv 2017
2019-05-07T11:21:29Z
2019-05-07T11:21:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv GUIMARÃES, C. C. et al. Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying. Revista Árvore, Viçosa, MG, v. 41, n. 4, 2017.
http://repositorio.ufla.br/jspui/handle/1/34012
identifier_str_mv GUIMARÃES, C. C. et al. Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying. Revista Árvore, Viçosa, MG, v. 41, n. 4, 2017.
url http://repositorio.ufla.br/jspui/handle/1/34012
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Investigações Florestais
publisher.none.fl_str_mv Sociedade de Investigações Florestais
dc.source.none.fl_str_mv Revista Árvore
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815438979313434624