Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/34012 |
Resumo: | The aim of this study was to evaluate DNA integrity and to identify ultrastructural changes in the primary root of Peltophorum dubium seeds subjected to drying, since these changes may reflect the germination capacity. Seeds were germinated and were dehydrated when reached 1 mm in length of the primary root, until reaching different moisture contents, being then rehydrated and evaluated in relation to survival. After this procedure, different points of interest (control, 20% and 10% moisture content) were selected to evaluate DNA and cell ultrastructure integrity in order to detect possible cellular changes in primary roots after dehydration. When dried down to 32% moisture, there was no influence on the growth resumption of the germinated seeds, but when they were dehydrated to 20% moisture, the survival was reduced to 67%, reaching nullity in the survival with drying to 8% moisture. By the evaluation of ultrastructural changes in root cells, it was observed that drying to approximately 20% moisture content marked the onset of damages by desiccation, both physiologically and ultrastructurally. Drying to levels close to 10% moisture content was not only responsible for total disorganization and rupture of membranes, but also promoted intracellular collapse. Loss of genomic DNA integrity was observed during drying. |
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Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to dryingMudanças ultra-estruturais e integridade do DNA genômico em sementes germinadas de peltophorum dubium submetidas à secagemDesiccation toleranceCellular alterationsDNA damageTolerância à dessecaçãoDanos ao DNAThe aim of this study was to evaluate DNA integrity and to identify ultrastructural changes in the primary root of Peltophorum dubium seeds subjected to drying, since these changes may reflect the germination capacity. Seeds were germinated and were dehydrated when reached 1 mm in length of the primary root, until reaching different moisture contents, being then rehydrated and evaluated in relation to survival. After this procedure, different points of interest (control, 20% and 10% moisture content) were selected to evaluate DNA and cell ultrastructure integrity in order to detect possible cellular changes in primary roots after dehydration. When dried down to 32% moisture, there was no influence on the growth resumption of the germinated seeds, but when they were dehydrated to 20% moisture, the survival was reduced to 67%, reaching nullity in the survival with drying to 8% moisture. By the evaluation of ultrastructural changes in root cells, it was observed that drying to approximately 20% moisture content marked the onset of damages by desiccation, both physiologically and ultrastructurally. Drying to levels close to 10% moisture content was not only responsible for total disorganization and rupture of membranes, but also promoted intracellular collapse. Loss of genomic DNA integrity was observed during drying.Objetivou-se com esse trabalho avaliar a integridade do DNA e identificar mudanças ultra-estruturais em raiz primária de sementes de Peltophorum dubium submetidas a secagem. Para tanto, sementes foram posta a germinar e ao atingirem 1 mm de comprimento da raiz primária, foram desidratadas, até atingirem diferentes graus de umidade, sendo em seguida reidratadas e avaliadas quanto à sobrevivência. Após esse procedimento foram selecionados diferentes pontos de interesse (controle, 20% e 10% de umidade) para avaliação da integridade do DNA e da ultraestrutura celular no intuito de detectar possíveis mudanças celulares nas raízes primárias após desidratação. Quando secas até 32% de umidade não houve influência na retomada do crescimento das sementes germinadas, mas ao serem desidratadas a 20% de umidade, a sobrevivência foi reduzida a 67% e a secagem a 8% de umidade concorreu para nulidade na sobrevivência. Pela avaliação de mudanças ultraestruturais em células da raiz observou-se que secagem a aproximadamente 20% de umidade marcou o início dos danos por dessecação, tanto fisiológica quanto ultraestruturalmente. Secagem a níveis próximos a 10% de umidade concorreu não somente para total desconfiguração e ruptura das membranas como também promoveu colapso intracelular. Foi observada perda da integridade do DNA genômico no decorrer da secagem.Sociedade de Investigações Florestais2019-05-07T11:21:29Z2019-05-07T11:21:29Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfGUIMARÃES, C. C. et al. Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying. Revista Árvore, Viçosa, MG, v. 41, n. 4, 2017.http://repositorio.ufla.br/jspui/handle/1/34012Revista Árvorereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessGuimarães, Cristiane CarvalhoOliveira, Julio MaiaFaria, José Marcio RochaSilva, Edvaldo Aparecido Amaral daeng2019-05-07T11:21:30Zoai:localhost:1/34012Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2019-05-07T11:21:30Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying Mudanças ultra-estruturais e integridade do DNA genômico em sementes germinadas de peltophorum dubium submetidas à secagem |
title |
Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying |
spellingShingle |
Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying Guimarães, Cristiane Carvalho Desiccation tolerance Cellular alterations DNA damage Tolerância à dessecação Danos ao DNA |
title_short |
Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying |
title_full |
Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying |
title_fullStr |
Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying |
title_full_unstemmed |
Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying |
title_sort |
Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying |
author |
Guimarães, Cristiane Carvalho |
author_facet |
Guimarães, Cristiane Carvalho Oliveira, Julio Maia Faria, José Marcio Rocha Silva, Edvaldo Aparecido Amaral da |
author_role |
author |
author2 |
Oliveira, Julio Maia Faria, José Marcio Rocha Silva, Edvaldo Aparecido Amaral da |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Guimarães, Cristiane Carvalho Oliveira, Julio Maia Faria, José Marcio Rocha Silva, Edvaldo Aparecido Amaral da |
dc.subject.por.fl_str_mv |
Desiccation tolerance Cellular alterations DNA damage Tolerância à dessecação Danos ao DNA |
topic |
Desiccation tolerance Cellular alterations DNA damage Tolerância à dessecação Danos ao DNA |
description |
The aim of this study was to evaluate DNA integrity and to identify ultrastructural changes in the primary root of Peltophorum dubium seeds subjected to drying, since these changes may reflect the germination capacity. Seeds were germinated and were dehydrated when reached 1 mm in length of the primary root, until reaching different moisture contents, being then rehydrated and evaluated in relation to survival. After this procedure, different points of interest (control, 20% and 10% moisture content) were selected to evaluate DNA and cell ultrastructure integrity in order to detect possible cellular changes in primary roots after dehydration. When dried down to 32% moisture, there was no influence on the growth resumption of the germinated seeds, but when they were dehydrated to 20% moisture, the survival was reduced to 67%, reaching nullity in the survival with drying to 8% moisture. By the evaluation of ultrastructural changes in root cells, it was observed that drying to approximately 20% moisture content marked the onset of damages by desiccation, both physiologically and ultrastructurally. Drying to levels close to 10% moisture content was not only responsible for total disorganization and rupture of membranes, but also promoted intracellular collapse. Loss of genomic DNA integrity was observed during drying. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2019-05-07T11:21:29Z 2019-05-07T11:21:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GUIMARÃES, C. C. et al. Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying. Revista Árvore, Viçosa, MG, v. 41, n. 4, 2017. http://repositorio.ufla.br/jspui/handle/1/34012 |
identifier_str_mv |
GUIMARÃES, C. C. et al. Ultrastructural changes and integrity of genomic dna in germinated seeds of Peltophorum dubium (Spreng.) taubert subjected to drying. Revista Árvore, Viçosa, MG, v. 41, n. 4, 2017. |
url |
http://repositorio.ufla.br/jspui/handle/1/34012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Investigações Florestais |
publisher.none.fl_str_mv |
Sociedade de Investigações Florestais |
dc.source.none.fl_str_mv |
Revista Árvore reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1815438979313434624 |