Cultivo in vitro de bambu em diferentes sistemas de propagação
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/31431 |
Resumo: | Among the new systems for cultivating plants, the bioreactors stood out as a method with suitable applicability, which may provide better conditions for the multiplication of seedlings in large scale and with a reduced cost. The aim of this study was to evaluate the number of established explants (free of bacterial or fungal contamination), as well as the multiplication and elongation of Bambusa vulgaris Schrad ex Wendl. sprouts in different cultivation systems. Explants derived from the in vitro establishment phase were transferred to three cultivation systems, which were composed by temporary immersion bioreactor (BIT), liquid-based in vitro cultivation (MEL) and in vitro cultivation in standard culture medium (STANDARD), where two sucrose concentrations were also tested (0 and 30 g.L-1). At 14 days, the rate of established explants was evaluated. From the explants considered alive and without signs of contamination, we evaluated the number and average length of sprouts. The greater number of established explants was observed in the BIT cultivation method in the absence of sucrose, and MEL when added 30 g.L-1 of sucrose. The BIT and MEL stood out showing greater average number of sprouts per explant. The STANDARD culture medium resulted in lower number of established explants, besides the lowest number of sprouts per explant. |
id |
UFLA_a9e3581f2fd4aa4462bdece99ac794ae |
---|---|
oai_identifier_str |
oai:localhost:1/31431 |
network_acronym_str |
UFLA |
network_name_str |
Repositório Institucional da UFLA |
repository_id_str |
|
spelling |
Cultivo in vitro de bambu em diferentes sistemas de propagaçãoIn vitro bamboo cultivation in different spread systemsBambusa vulgarisBiorreatorMicropropagaçãoSacaroseBioreactorMicropropagationSucroseAmong the new systems for cultivating plants, the bioreactors stood out as a method with suitable applicability, which may provide better conditions for the multiplication of seedlings in large scale and with a reduced cost. The aim of this study was to evaluate the number of established explants (free of bacterial or fungal contamination), as well as the multiplication and elongation of Bambusa vulgaris Schrad ex Wendl. sprouts in different cultivation systems. Explants derived from the in vitro establishment phase were transferred to three cultivation systems, which were composed by temporary immersion bioreactor (BIT), liquid-based in vitro cultivation (MEL) and in vitro cultivation in standard culture medium (STANDARD), where two sucrose concentrations were also tested (0 and 30 g.L-1). At 14 days, the rate of established explants was evaluated. From the explants considered alive and without signs of contamination, we evaluated the number and average length of sprouts. The greater number of established explants was observed in the BIT cultivation method in the absence of sucrose, and MEL when added 30 g.L-1 of sucrose. The BIT and MEL stood out showing greater average number of sprouts per explant. The STANDARD culture medium resulted in lower number of established explants, besides the lowest number of sprouts per explant.Dentre os novos sistemas de cultivo de plantas, os biorreatores apresentam destaque como um método de adequada aplicabilidade, o que pode proporcionar melhores condições para a multiplicação de mudas em larga escala e com custo reduzido. O objetivo do presente estudo foi avaliar o número de explantes estabelecidos (isentos de contaminação bacteriana ou fúngica), a multiplicação e o alongamento de brotos de Bambusa vulgaris Schrad ex Wendl. em diferentes sistemas de cultivo. Explantes oriundos da fase de estabelecimentoin vitroforam transferidos para três sistemas de cultivo, sendo eles compostos por biorreator de imersão temporária (BIT), cultivo in vitro em meio líquido(MEL) e cultivo in vitro em meio de cultura padrão (PADRÃO), onde também foram testadas duas concentrações de sacarose (0 e 30 g.L-1). Aos 14 dias, foi avaliada a taxa de explantes estabelecidos. Dos explantes considerados vivos e que não apresentaram contaminação foram avaliados o número e o comprimento médio dos brotos. O maior número de explantes estabelecidos foi observado no método de cultivo tipo BIT na ausência de sacarose, e MEL quando suplementado 30 g.L-1 de sacarose. O BIT e MEL se destacaram apresentando maior número médio de brotos por explante. O meio de cultura PADRÃO resultou em menor número de explantes estabelecidos e o menor número de brotos por explante.2018-10-24T13:54:57Z2018-10-24T13:54:57Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfRIBEIRO, A. dos S. et al. Cultivo in vitro de bambu em diferentes sistemas de propagação. Nativa, Sinop, v. 4, n. 1, p. 15-18, jan./fev. 2016.http://repositorio.ufla.br/jspui/handle/1/31431http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessRibeiro, Anatálya dos SantosBrondani, Gilvano EblingTormen, Gabriela Cristina RechFigueiredo, Alexssandra Jéssica Rondon deporreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2018-10-24T13:54:57Zoai:localhost:1/31431Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-10-24T13:54:57Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Cultivo in vitro de bambu em diferentes sistemas de propagação In vitro bamboo cultivation in different spread systems |
title |
Cultivo in vitro de bambu em diferentes sistemas de propagação |
spellingShingle |
Cultivo in vitro de bambu em diferentes sistemas de propagação Ribeiro, Anatálya dos Santos Bambusa vulgaris Biorreator Micropropagação Sacarose Bioreactor Micropropagation Sucrose |
title_short |
Cultivo in vitro de bambu em diferentes sistemas de propagação |
title_full |
Cultivo in vitro de bambu em diferentes sistemas de propagação |
title_fullStr |
Cultivo in vitro de bambu em diferentes sistemas de propagação |
title_full_unstemmed |
Cultivo in vitro de bambu em diferentes sistemas de propagação |
title_sort |
Cultivo in vitro de bambu em diferentes sistemas de propagação |
author |
Ribeiro, Anatálya dos Santos |
author_facet |
Ribeiro, Anatálya dos Santos Brondani, Gilvano Ebling Tormen, Gabriela Cristina Rech Figueiredo, Alexssandra Jéssica Rondon de |
author_role |
author |
author2 |
Brondani, Gilvano Ebling Tormen, Gabriela Cristina Rech Figueiredo, Alexssandra Jéssica Rondon de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Anatálya dos Santos Brondani, Gilvano Ebling Tormen, Gabriela Cristina Rech Figueiredo, Alexssandra Jéssica Rondon de |
dc.subject.por.fl_str_mv |
Bambusa vulgaris Biorreator Micropropagação Sacarose Bioreactor Micropropagation Sucrose |
topic |
Bambusa vulgaris Biorreator Micropropagação Sacarose Bioreactor Micropropagation Sucrose |
description |
Among the new systems for cultivating plants, the bioreactors stood out as a method with suitable applicability, which may provide better conditions for the multiplication of seedlings in large scale and with a reduced cost. The aim of this study was to evaluate the number of established explants (free of bacterial or fungal contamination), as well as the multiplication and elongation of Bambusa vulgaris Schrad ex Wendl. sprouts in different cultivation systems. Explants derived from the in vitro establishment phase were transferred to three cultivation systems, which were composed by temporary immersion bioreactor (BIT), liquid-based in vitro cultivation (MEL) and in vitro cultivation in standard culture medium (STANDARD), where two sucrose concentrations were also tested (0 and 30 g.L-1). At 14 days, the rate of established explants was evaluated. From the explants considered alive and without signs of contamination, we evaluated the number and average length of sprouts. The greater number of established explants was observed in the BIT cultivation method in the absence of sucrose, and MEL when added 30 g.L-1 of sucrose. The BIT and MEL stood out showing greater average number of sprouts per explant. The STANDARD culture medium resulted in lower number of established explants, besides the lowest number of sprouts per explant. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2018-10-24T13:54:57Z 2018-10-24T13:54:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
RIBEIRO, A. dos S. et al. Cultivo in vitro de bambu em diferentes sistemas de propagação. Nativa, Sinop, v. 4, n. 1, p. 15-18, jan./fev. 2016. http://repositorio.ufla.br/jspui/handle/1/31431 |
identifier_str_mv |
RIBEIRO, A. dos S. et al. Cultivo in vitro de bambu em diferentes sistemas de propagação. Nativa, Sinop, v. 4, n. 1, p. 15-18, jan./fev. 2016. |
url |
http://repositorio.ufla.br/jspui/handle/1/31431 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835109735268352 |