Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/39227 |
Resumo: | Objective: The purpose of this work was to evaluate the reproductive performance and the toxicity of DMSO cryoprotectants and methanol toward semen of Nile tilapia (Oreochromis niloticus), fed using diets containing different Crude Protein (CP) levels. Study Design: completely randomized design. Place and Duration: The experiment was performed for 90 days, at the Hydrobiology and Fish Culture Station of Eletrobrás Furnas, in São José da Barra, State of Minas Gerais, Brazil. Methodology: 15 broodstock males with around 30 months old were used. They were stocked in ten reservoirs of storage capacity around 8 cubic meters, in which treatments consisted of five diets containing different levels of crude protein (32, 34, 36, 38 and 40%) and 9.5kcal/kg of digestible energy per gram of protein. For the in-natura semen, the statistical analyses were carried out using data obtained from a completely randomized design, while for diluted semen, the completely randomized design was also used but, in 5×2 factorial scheme, representing 5 levels of protein and 2 cryoprotectants. Results: The hepatosomatic index (HSI), gonadosomatic index (GSI), rate and duration of Nile tilapia males in-natura semen motility were not influenced (p>0.05) by the crude protein levels in the diets. After dilution, significant differences were observed for motility (p=0.053) and motility duration (p=0.021). The effects of third and fourth degree occurred for the rate (p=0.0349) and duration (p=0.0220). As these adjustments do not allow a biological interpretation, the Tukey multiple comparison test was used. The diet containing 36% of crude protein level showed mean motility rate similar (p>0.05) to those of treatments containing 32, 34 and 40% of crude protein and mean motility rate inferior (p<0.05) to treatment containing 38% of crude protein. For motility duration, fish fed using diet containing 38% and 40% of crude protein showed significant differences (p<0.05) to only the group of fish fed using diet containing 34% of crude protein. Conclusion: Diet containing 32, 38 and 40% of crude protein level does not deteriorate the semen quality, so that may be used for feeding tilapia broodstocks. As protein is an expensive input, we can infer that 32% of crude protein is sufficient to determine good quality gametes. |
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Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus)Fish - ReproductionSemenCryopreservationFish-malePeixes - ReproduçãoQualidade espermáticaTilápia do NiloObjective: The purpose of this work was to evaluate the reproductive performance and the toxicity of DMSO cryoprotectants and methanol toward semen of Nile tilapia (Oreochromis niloticus), fed using diets containing different Crude Protein (CP) levels. Study Design: completely randomized design. Place and Duration: The experiment was performed for 90 days, at the Hydrobiology and Fish Culture Station of Eletrobrás Furnas, in São José da Barra, State of Minas Gerais, Brazil. Methodology: 15 broodstock males with around 30 months old were used. They were stocked in ten reservoirs of storage capacity around 8 cubic meters, in which treatments consisted of five diets containing different levels of crude protein (32, 34, 36, 38 and 40%) and 9.5kcal/kg of digestible energy per gram of protein. For the in-natura semen, the statistical analyses were carried out using data obtained from a completely randomized design, while for diluted semen, the completely randomized design was also used but, in 5×2 factorial scheme, representing 5 levels of protein and 2 cryoprotectants. Results: The hepatosomatic index (HSI), gonadosomatic index (GSI), rate and duration of Nile tilapia males in-natura semen motility were not influenced (p>0.05) by the crude protein levels in the diets. After dilution, significant differences were observed for motility (p=0.053) and motility duration (p=0.021). The effects of third and fourth degree occurred for the rate (p=0.0349) and duration (p=0.0220). As these adjustments do not allow a biological interpretation, the Tukey multiple comparison test was used. The diet containing 36% of crude protein level showed mean motility rate similar (p>0.05) to those of treatments containing 32, 34 and 40% of crude protein and mean motility rate inferior (p<0.05) to treatment containing 38% of crude protein. For motility duration, fish fed using diet containing 38% and 40% of crude protein showed significant differences (p<0.05) to only the group of fish fed using diet containing 34% of crude protein. Conclusion: Diet containing 32, 38 and 40% of crude protein level does not deteriorate the semen quality, so that may be used for feeding tilapia broodstocks. As protein is an expensive input, we can infer that 32% of crude protein is sufficient to determine good quality gametes.Journal of Experimental Agriculture International2020-03-06T18:44:20Z2020-03-06T18:44:20Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfOLIVEIRA, M. M. de et al. Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus). American Journal of Experimental Agriculture, [S. I.], v. 5, n. 3, p. 192-199, 2015.http://repositorio.ufla.br/jspui/handle/1/39227American Journal of Experimental Agriculturereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessOliveira, Marinez Moraes deFerreira, Mônica RodriguesGoulart, Marcilia BarbosaFelizardo, Viviane de OliveiraMurgas, Luis David SolisAndrade, Estefânia de SouzaAllaman, Ivan BezerraVeras, Galileu CrovattoCosta, Diego Vicente daRosa, Priscila Vieira eeng2020-03-06T18:45:13Zoai:localhost:1/39227Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-03-06T18:45:13Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus) |
title |
Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus) |
spellingShingle |
Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus) Oliveira, Marinez Moraes de Fish - Reproduction Semen Cryopreservation Fish-male Peixes - Reprodução Qualidade espermática Tilápia do Nilo |
title_short |
Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus) |
title_full |
Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus) |
title_fullStr |
Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus) |
title_full_unstemmed |
Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus) |
title_sort |
Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus) |
author |
Oliveira, Marinez Moraes de |
author_facet |
Oliveira, Marinez Moraes de Ferreira, Mônica Rodrigues Goulart, Marcilia Barbosa Felizardo, Viviane de Oliveira Murgas, Luis David Solis Andrade, Estefânia de Souza Allaman, Ivan Bezerra Veras, Galileu Crovatto Costa, Diego Vicente da Rosa, Priscila Vieira e |
author_role |
author |
author2 |
Ferreira, Mônica Rodrigues Goulart, Marcilia Barbosa Felizardo, Viviane de Oliveira Murgas, Luis David Solis Andrade, Estefânia de Souza Allaman, Ivan Bezerra Veras, Galileu Crovatto Costa, Diego Vicente da Rosa, Priscila Vieira e |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Marinez Moraes de Ferreira, Mônica Rodrigues Goulart, Marcilia Barbosa Felizardo, Viviane de Oliveira Murgas, Luis David Solis Andrade, Estefânia de Souza Allaman, Ivan Bezerra Veras, Galileu Crovatto Costa, Diego Vicente da Rosa, Priscila Vieira e |
dc.subject.por.fl_str_mv |
Fish - Reproduction Semen Cryopreservation Fish-male Peixes - Reprodução Qualidade espermática Tilápia do Nilo |
topic |
Fish - Reproduction Semen Cryopreservation Fish-male Peixes - Reprodução Qualidade espermática Tilápia do Nilo |
description |
Objective: The purpose of this work was to evaluate the reproductive performance and the toxicity of DMSO cryoprotectants and methanol toward semen of Nile tilapia (Oreochromis niloticus), fed using diets containing different Crude Protein (CP) levels. Study Design: completely randomized design. Place and Duration: The experiment was performed for 90 days, at the Hydrobiology and Fish Culture Station of Eletrobrás Furnas, in São José da Barra, State of Minas Gerais, Brazil. Methodology: 15 broodstock males with around 30 months old were used. They were stocked in ten reservoirs of storage capacity around 8 cubic meters, in which treatments consisted of five diets containing different levels of crude protein (32, 34, 36, 38 and 40%) and 9.5kcal/kg of digestible energy per gram of protein. For the in-natura semen, the statistical analyses were carried out using data obtained from a completely randomized design, while for diluted semen, the completely randomized design was also used but, in 5×2 factorial scheme, representing 5 levels of protein and 2 cryoprotectants. Results: The hepatosomatic index (HSI), gonadosomatic index (GSI), rate and duration of Nile tilapia males in-natura semen motility were not influenced (p>0.05) by the crude protein levels in the diets. After dilution, significant differences were observed for motility (p=0.053) and motility duration (p=0.021). The effects of third and fourth degree occurred for the rate (p=0.0349) and duration (p=0.0220). As these adjustments do not allow a biological interpretation, the Tukey multiple comparison test was used. The diet containing 36% of crude protein level showed mean motility rate similar (p>0.05) to those of treatments containing 32, 34 and 40% of crude protein and mean motility rate inferior (p<0.05) to treatment containing 38% of crude protein. For motility duration, fish fed using diet containing 38% and 40% of crude protein showed significant differences (p<0.05) to only the group of fish fed using diet containing 34% of crude protein. Conclusion: Diet containing 32, 38 and 40% of crude protein level does not deteriorate the semen quality, so that may be used for feeding tilapia broodstocks. As protein is an expensive input, we can infer that 32% of crude protein is sufficient to determine good quality gametes. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2020-03-06T18:44:20Z 2020-03-06T18:44:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
OLIVEIRA, M. M. de et al. Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus). American Journal of Experimental Agriculture, [S. I.], v. 5, n. 3, p. 192-199, 2015. http://repositorio.ufla.br/jspui/handle/1/39227 |
identifier_str_mv |
OLIVEIRA, M. M. de et al. Effect Crude Protein Levels on the Broodstock Spermatic Quality of Nile tilapia (Oreochromis niloticus). American Journal of Experimental Agriculture, [S. I.], v. 5, n. 3, p. 192-199, 2015. |
url |
http://repositorio.ufla.br/jspui/handle/1/39227 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Journal of Experimental Agriculture International |
publisher.none.fl_str_mv |
Journal of Experimental Agriculture International |
dc.source.none.fl_str_mv |
American Journal of Experimental Agriculture reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835193569968128 |