Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/30202 |
Resumo: | Plant flavonoids have been increasingly studied for its importance in plant metabolism, inter-species interactions and human health-promoting effects. Each botanical species has a distinct flavonoid profile, and as such, this work aimed to develop and apply a high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method to quantify specific flavonoids in different plants and parts. Six flavonoids (isoquercitrin, quercetin, naringin, naringenin, hesperidin and rutin), two isoflavonoids (daidzin and daidzein) and one coumarin (umbelliferone) were targeted. Calibration curves showed good correlations (R2 > 0.994) and limits of quantification (≤ 500 ng mL-1). The method was applied for detection of analytes in common bean seeds (Phaseolus vulgaris), soybean leaves (Glycine max), and sour orange (Citrus aurantium), sweet orange (Citrus sinensis), and Tahiti lime (Citrus latifolia) flavedo after ultrasound assisted acidic hydroalcoholic extraction. The method was tuned for quick and efficient extraction. Principal component analysis was helpful for the discrimination of matrices based on their flavonoid profiles. |
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Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matricesFlavonoidQuantitative analysisHigh performance liquid chromatography - electrospray tandem mass spectrometry (HPLC-ESI-MS-MS)Plant flavonoids have been increasingly studied for its importance in plant metabolism, inter-species interactions and human health-promoting effects. Each botanical species has a distinct flavonoid profile, and as such, this work aimed to develop and apply a high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method to quantify specific flavonoids in different plants and parts. Six flavonoids (isoquercitrin, quercetin, naringin, naringenin, hesperidin and rutin), two isoflavonoids (daidzin and daidzein) and one coumarin (umbelliferone) were targeted. Calibration curves showed good correlations (R2 > 0.994) and limits of quantification (≤ 500 ng mL-1). The method was applied for detection of analytes in common bean seeds (Phaseolus vulgaris), soybean leaves (Glycine max), and sour orange (Citrus aurantium), sweet orange (Citrus sinensis), and Tahiti lime (Citrus latifolia) flavedo after ultrasound assisted acidic hydroalcoholic extraction. The method was tuned for quick and efficient extraction. Principal component analysis was helpful for the discrimination of matrices based on their flavonoid profiles.Sociedade Brasileira de Química2018-08-27T19:31:48Z2018-08-27T19:31:48Z2016-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPERLATTI, B. et al. Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices. Journal of the Brazilian Chemical Society, São Paulo, v. 27, n. 3, Mar. 2016.http://repositorio.ufla.br/jspui/handle/1/30202Journal of the Brazilian Chemical Societyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAPerlatti, BrunoFernandes, João B.Silva, Maria F. G. F.Ardila, Jorge A.Carneiro, Renato L.Souza, Bruno H. S.Costa, Eduardo N.Eduardo, Wellington I.Boiça Junior, Arlindo L.Forim, Moacir R.info:eu-repo/semantics/openAccesspor2023-05-09T11:53:22Zoai:localhost:1/30202Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-09T11:53:22Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices |
title |
Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices |
spellingShingle |
Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices Perlatti, Bruno Flavonoid Quantitative analysis High performance liquid chromatography - electrospray tandem mass spectrometry (HPLC-ESI-MS-MS) |
title_short |
Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices |
title_full |
Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices |
title_fullStr |
Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices |
title_full_unstemmed |
Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices |
title_sort |
Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices |
author |
Perlatti, Bruno |
author_facet |
Perlatti, Bruno Fernandes, João B. Silva, Maria F. G. F. Ardila, Jorge A. Carneiro, Renato L. Souza, Bruno H. S. Costa, Eduardo N. Eduardo, Wellington I. Boiça Junior, Arlindo L. Forim, Moacir R. |
author_role |
author |
author2 |
Fernandes, João B. Silva, Maria F. G. F. Ardila, Jorge A. Carneiro, Renato L. Souza, Bruno H. S. Costa, Eduardo N. Eduardo, Wellington I. Boiça Junior, Arlindo L. Forim, Moacir R. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Perlatti, Bruno Fernandes, João B. Silva, Maria F. G. F. Ardila, Jorge A. Carneiro, Renato L. Souza, Bruno H. S. Costa, Eduardo N. Eduardo, Wellington I. Boiça Junior, Arlindo L. Forim, Moacir R. |
dc.subject.por.fl_str_mv |
Flavonoid Quantitative analysis High performance liquid chromatography - electrospray tandem mass spectrometry (HPLC-ESI-MS-MS) |
topic |
Flavonoid Quantitative analysis High performance liquid chromatography - electrospray tandem mass spectrometry (HPLC-ESI-MS-MS) |
description |
Plant flavonoids have been increasingly studied for its importance in plant metabolism, inter-species interactions and human health-promoting effects. Each botanical species has a distinct flavonoid profile, and as such, this work aimed to develop and apply a high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method to quantify specific flavonoids in different plants and parts. Six flavonoids (isoquercitrin, quercetin, naringin, naringenin, hesperidin and rutin), two isoflavonoids (daidzin and daidzein) and one coumarin (umbelliferone) were targeted. Calibration curves showed good correlations (R2 > 0.994) and limits of quantification (≤ 500 ng mL-1). The method was applied for detection of analytes in common bean seeds (Phaseolus vulgaris), soybean leaves (Glycine max), and sour orange (Citrus aurantium), sweet orange (Citrus sinensis), and Tahiti lime (Citrus latifolia) flavedo after ultrasound assisted acidic hydroalcoholic extraction. The method was tuned for quick and efficient extraction. Principal component analysis was helpful for the discrimination of matrices based on their flavonoid profiles. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03 2018-08-27T19:31:48Z 2018-08-27T19:31:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PERLATTI, B. et al. Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices. Journal of the Brazilian Chemical Society, São Paulo, v. 27, n. 3, Mar. 2016. http://repositorio.ufla.br/jspui/handle/1/30202 |
identifier_str_mv |
PERLATTI, B. et al. Application of a quantitative HPLC-ESI-MS/MS method for flavonoids in different vegetable matrices. Journal of the Brazilian Chemical Society, São Paulo, v. 27, n. 3, Mar. 2016. |
url |
http://repositorio.ufla.br/jspui/handle/1/30202 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835222339747840 |