Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith

Detalhes bibliográficos
Autor(a) principal: Avelar, Maria Lopes Martins
Data de Publicação: 2022
Outros Autores: Moscardini, Bruno Alves, Souza, Denys Matheus Santana Costa, Molinari, Letícia Vaz, Gonçalves, Douglas Santos, Faria, Júlio Cezar Tannure, Brondani, Gilvano Ebling
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/50908
Resumo: We aimed to evaluate the in vitro establishment of nodal segments of Eucalyptus pilularis Smith considering two origins of tissues (Or1 - epicormic shoots collected from pruned branches of selected adult trees; Or2 - shoots collected from seminal mini-stumps) and four inoculation methods (Me1 - culture medium supplemented with 0.5 g L-1 activated charcoal; Me2 - culture medium supplemented with 800 mg L-1 PVP30; Me3 - exposure to light for 30 days; Me4 - exposure to dark for 7 days). At 30 days after the in vitro inoculation of tissues, there was no establishment of tissues from epicormic shoots (Or1). Or2 resulted in lower percentages of tissue oxidation and contamination by microorganisms, in addition to having presented establishment and formation of shoots. Me1 resulted in a lower mean tissue oxidation, although it differed statistically only from Me4. An origin of the tissues of ontogenetic age was a determining factor for the successful in vitro establishment of E. pilularis. The use of the Or2 origin and the Me1, Me2, and Me3 methods are recommended to reduce phenolic oxidation of tissues in the in vitro establishment.
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spelling Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smithdade ontogenética e métodos de inoculação para o estabelecimento in vitrodeEucalyptus pilularis SmithAntioxidant agentsExposure to lightEpicormic shootsTissue oxidationAgentes antioxidantesExposição à luminosidadeBrotos epicórmicosOxidação de tecidosWe aimed to evaluate the in vitro establishment of nodal segments of Eucalyptus pilularis Smith considering two origins of tissues (Or1 - epicormic shoots collected from pruned branches of selected adult trees; Or2 - shoots collected from seminal mini-stumps) and four inoculation methods (Me1 - culture medium supplemented with 0.5 g L-1 activated charcoal; Me2 - culture medium supplemented with 800 mg L-1 PVP30; Me3 - exposure to light for 30 days; Me4 - exposure to dark for 7 days). At 30 days after the in vitro inoculation of tissues, there was no establishment of tissues from epicormic shoots (Or1). Or2 resulted in lower percentages of tissue oxidation and contamination by microorganisms, in addition to having presented establishment and formation of shoots. Me1 resulted in a lower mean tissue oxidation, although it differed statistically only from Me4. An origin of the tissues of ontogenetic age was a determining factor for the successful in vitro establishment of E. pilularis. The use of the Or2 origin and the Me1, Me2, and Me3 methods are recommended to reduce phenolic oxidation of tissues in the in vitro establishment.Objetivou-se avaliar o estabelecimento in vitro de segmentos nodais de Eucalyptus pilularisconsiderando duas origens de tecidos (Or1 - brotos epicórmicos coletados em galhos podados de matrizes adultas e Or2 - brotos coletados de minicepas seminais) e quatro métodos de inoculação (Me1 - meio de cultura suplementado com 0,5 g L-1 de carvão ativado, Me2 - meio de cultura suplementado com 800 mg L-1 de PVP30, Me3 - exposição à luminosidade por trinta dias e Me4 - exposição em ambiente com ausência de luminosidade por sete dias). Aos 30 dias após a inoculação in vitro dos tecidos, constatou-se que não houve estabelecimento de tecidos oriundos da Or1. A utilização de segmentos nodais provenientes da Or2 resultou em menores percentuais de oxidação e de contaminação por microrganismos, além de ter apresentado estabelecimento e emissão de brotos. O uso do Me1 resultou em menor média de oxidação, embora tenha diferido estatisticamente somente do Me4. A origem dos tecidos associada à idade ontogenética foi um fator determinante para o sucesso do estabelecimento in vitro de E. pilularis. Recomenda-se a utilização da Or2 e dos métodos Me1, Me2 e Me3 a fim de reduzir a oxidação fenólica dos tecidos durante o estabelecimento in vitro.Universidade Federal do Mato Grosso2022-08-09T19:32:20Z2022-08-09T19:32:20Z2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfAVELAR, M. L. M. et al. Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith. Nativa, Sinop, v. 10, n. 1, p. 40-46, 2022.http://repositorio.ufla.br/jspui/handle/1/50908Nativareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAvelar, Maria Lopes MartinsMoscardini, Bruno AlvesSouza, Denys Matheus Santana CostaMolinari, Letícia VazGonçalves, Douglas SantosFaria, Júlio Cezar TannureBrondani, Gilvano Eblingeng2022-08-09T19:32:20Zoai:localhost:1/50908Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2022-08-09T19:32:20Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith
dade ontogenética e métodos de inoculação para o estabelecimento in vitrodeEucalyptus pilularis Smith
title Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith
spellingShingle Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith
Avelar, Maria Lopes Martins
Antioxidant agents
Exposure to light
Epicormic shoots
Tissue oxidation
Agentes antioxidantes
Exposição à luminosidade
Brotos epicórmicos
Oxidação de tecidos
title_short Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith
title_full Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith
title_fullStr Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith
title_full_unstemmed Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith
title_sort Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith
author Avelar, Maria Lopes Martins
author_facet Avelar, Maria Lopes Martins
Moscardini, Bruno Alves
Souza, Denys Matheus Santana Costa
Molinari, Letícia Vaz
Gonçalves, Douglas Santos
Faria, Júlio Cezar Tannure
Brondani, Gilvano Ebling
author_role author
author2 Moscardini, Bruno Alves
Souza, Denys Matheus Santana Costa
Molinari, Letícia Vaz
Gonçalves, Douglas Santos
Faria, Júlio Cezar Tannure
Brondani, Gilvano Ebling
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Avelar, Maria Lopes Martins
Moscardini, Bruno Alves
Souza, Denys Matheus Santana Costa
Molinari, Letícia Vaz
Gonçalves, Douglas Santos
Faria, Júlio Cezar Tannure
Brondani, Gilvano Ebling
dc.subject.por.fl_str_mv Antioxidant agents
Exposure to light
Epicormic shoots
Tissue oxidation
Agentes antioxidantes
Exposição à luminosidade
Brotos epicórmicos
Oxidação de tecidos
topic Antioxidant agents
Exposure to light
Epicormic shoots
Tissue oxidation
Agentes antioxidantes
Exposição à luminosidade
Brotos epicórmicos
Oxidação de tecidos
description We aimed to evaluate the in vitro establishment of nodal segments of Eucalyptus pilularis Smith considering two origins of tissues (Or1 - epicormic shoots collected from pruned branches of selected adult trees; Or2 - shoots collected from seminal mini-stumps) and four inoculation methods (Me1 - culture medium supplemented with 0.5 g L-1 activated charcoal; Me2 - culture medium supplemented with 800 mg L-1 PVP30; Me3 - exposure to light for 30 days; Me4 - exposure to dark for 7 days). At 30 days after the in vitro inoculation of tissues, there was no establishment of tissues from epicormic shoots (Or1). Or2 resulted in lower percentages of tissue oxidation and contamination by microorganisms, in addition to having presented establishment and formation of shoots. Me1 resulted in a lower mean tissue oxidation, although it differed statistically only from Me4. An origin of the tissues of ontogenetic age was a determining factor for the successful in vitro establishment of E. pilularis. The use of the Or2 origin and the Me1, Me2, and Me3 methods are recommended to reduce phenolic oxidation of tissues in the in vitro establishment.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-09T19:32:20Z
2022-08-09T19:32:20Z
2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv AVELAR, M. L. M. et al. Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith. Nativa, Sinop, v. 10, n. 1, p. 40-46, 2022.
http://repositorio.ufla.br/jspui/handle/1/50908
identifier_str_mv AVELAR, M. L. M. et al. Ontogenetic age and inoculation methods for the in vitro establishment of Eucalyptus pilularis Smith. Nativa, Sinop, v. 10, n. 1, p. 40-46, 2022.
url http://repositorio.ufla.br/jspui/handle/1/50908
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Mato Grosso
publisher.none.fl_str_mv Universidade Federal do Mato Grosso
dc.source.none.fl_str_mv Nativa
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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