Micropropagação de Dioscorea multiflora Grised

Detalhes bibliográficos
Autor(a) principal: Souza, Ana Valéria de
Data de Publicação: 2011
Outros Autores: Bertoni, Bianca Waléria, França, Suzelei de Castro, Pereira, Ana Maria Soares
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000100011
http://repositorio.ufla.br/jspui/handle/1/7357
Resumo: Dioscorea multiflora is a plant native to southern Brazil that produces diosgenin as a major secondary metabolite, a substance which is used by the pharmaceutical industry for the production of cortisone and substances with contraceptive action. The objective of this work was to optimize the micropropagation protocol of D. multiflora, for the production of seedlings on a commercial scale. Nodal segments subcultured in solid MS medium were transferred for multiplication to MS medium supplemented with BAP (0.01, 0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with 0.1 mg L-1 or 0.5 mg L-1 BAP plus different concentrations of sucrose (2, 4, 6, 8 and 10%). For rooting, the shoots were cultured on MS medium supplemented with IBA (0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with NAA (0.1, 0.5, 1.0 and 3.0 mg L-1). A completely randomized design was used with treatment consisting of 3 replicates with 10 buckets per plot. Seedlings with and without roots were acclimatized in a greenhouse. The best results of multiplication and rooting were obtained in MS medium + 0.1 mg L-1 BAP (80%) and in MS medium + 1.0 mg L-1 IBA (42.6%), respectively. There was no difference in the survival percentage of seedlings in vitro and during ex vitro acclimatization (75%). The micropropagation protocol for production of D. multiflora is effective and can be used for commercial production.
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spelling Micropropagação de Dioscorea multiflora GrisedMicropropagation of Dioscorea multiflora GrisedDiosgeninaCultivo in vitroAclimataçãoDiosgeninIn vitro cultivationAcclimatizationDioscorea multiflora is a plant native to southern Brazil that produces diosgenin as a major secondary metabolite, a substance which is used by the pharmaceutical industry for the production of cortisone and substances with contraceptive action. The objective of this work was to optimize the micropropagation protocol of D. multiflora, for the production of seedlings on a commercial scale. Nodal segments subcultured in solid MS medium were transferred for multiplication to MS medium supplemented with BAP (0.01, 0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with 0.1 mg L-1 or 0.5 mg L-1 BAP plus different concentrations of sucrose (2, 4, 6, 8 and 10%). For rooting, the shoots were cultured on MS medium supplemented with IBA (0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with NAA (0.1, 0.5, 1.0 and 3.0 mg L-1). A completely randomized design was used with treatment consisting of 3 replicates with 10 buckets per plot. Seedlings with and without roots were acclimatized in a greenhouse. The best results of multiplication and rooting were obtained in MS medium + 0.1 mg L-1 BAP (80%) and in MS medium + 1.0 mg L-1 IBA (42.6%), respectively. There was no difference in the survival percentage of seedlings in vitro and during ex vitro acclimatization (75%). The micropropagation protocol for production of D. multiflora is effective and can be used for commercial production.Dioscorea multiflora uma planta nativa do Sul do Brasil produz a diosgenina como metabólito secundário majoritário, uma substância potencialmente usada pela indústria farmacêutica para a produção de cortisona e substâncias com ação contraceptiva. Objetivou-se, neste trabalho otimizar o protocolo de micropropagação de D. multiflora, visando a produção de mudas em escala comercial. Segmentos nodais subcultivados em meio MS sólido foram transferidos para multiplicação em meio MS suplementado com BAP (0,01; 0,1; 0,5; 1,0 e 3,0 mg L-1)e meio MS suplementado com 0,1 mg L-1 ou 0,5 mg L-1 de BAP acrescido de diferentes concentrações de sacarose (2, 4, 6, 8 e 10%). Para o enraizamento, as brotações foram cultivadas em meio MS suplementado com AIB (0,1; 0,5; 1,0 e 3,0 mg L-1) e meio MS suplementado com ANA (0,1; 0,5; 1,0 e 3,0 mg L-1). Os experimentos in vitro foram instalados em delineamento experimental inteiramente casualizado e cada tratamento constituiu-se de 3 repetições e 10 cubetas/parcela. Plântulas com e sem raízes foram aclimatizadas em casa de vegetação. Melhores resultados de multiplicação e enraizamento foram obtidos em meio MS + 0,1 mg L-1 de BAP (80%) e em meio MS + 1,0 mg L-1 de AIB (42,6%), respectivamente. Não houve diferença quanto à porcentagem de sobrevivência das plântulas in vitro e ex vitro durante a aclimatização (75%). O protocolo de micropropagação para D. Multiflora é efetivo e pode ser usado para a produção em escala comercial.Editora da Universidade Federal de Lavras2011-02-012015-04-30T13:36:04Z2015-04-30T13:36:04Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000100011SOUZA, A. V. de et al. Micropropagação de Dioscorea multiflora Grised. Ciência e Agrotecnologia, Lavras, v. 35, n. 1, p. 92-98, jan./fev. 2011.http://repositorio.ufla.br/jspui/handle/1/7357Ciência e Agrotecnologia v.35 n.1 2011reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLASouza, Ana Valéria deBertoni, Bianca WalériaFrança, Suzelei de CastroPereira, Ana Maria Soaresporinfo:eu-repo/semantics/openAccess2016-07-21T18:04:34Zoai:localhost:1/7357Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-07-21T18:04:34Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Micropropagação de Dioscorea multiflora Grised
Micropropagation of Dioscorea multiflora Grised
title Micropropagação de Dioscorea multiflora Grised
spellingShingle Micropropagação de Dioscorea multiflora Grised
Souza, Ana Valéria de
Diosgenina
Cultivo in vitro
Aclimatação
Diosgenin
In vitro cultivation
Acclimatization
title_short Micropropagação de Dioscorea multiflora Grised
title_full Micropropagação de Dioscorea multiflora Grised
title_fullStr Micropropagação de Dioscorea multiflora Grised
title_full_unstemmed Micropropagação de Dioscorea multiflora Grised
title_sort Micropropagação de Dioscorea multiflora Grised
author Souza, Ana Valéria de
author_facet Souza, Ana Valéria de
Bertoni, Bianca Waléria
França, Suzelei de Castro
Pereira, Ana Maria Soares
author_role author
author2 Bertoni, Bianca Waléria
França, Suzelei de Castro
Pereira, Ana Maria Soares
author2_role author
author
author
dc.contributor.author.fl_str_mv Souza, Ana Valéria de
Bertoni, Bianca Waléria
França, Suzelei de Castro
Pereira, Ana Maria Soares
dc.subject.por.fl_str_mv Diosgenina
Cultivo in vitro
Aclimatação
Diosgenin
In vitro cultivation
Acclimatization
topic Diosgenina
Cultivo in vitro
Aclimatação
Diosgenin
In vitro cultivation
Acclimatization
description Dioscorea multiflora is a plant native to southern Brazil that produces diosgenin as a major secondary metabolite, a substance which is used by the pharmaceutical industry for the production of cortisone and substances with contraceptive action. The objective of this work was to optimize the micropropagation protocol of D. multiflora, for the production of seedlings on a commercial scale. Nodal segments subcultured in solid MS medium were transferred for multiplication to MS medium supplemented with BAP (0.01, 0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with 0.1 mg L-1 or 0.5 mg L-1 BAP plus different concentrations of sucrose (2, 4, 6, 8 and 10%). For rooting, the shoots were cultured on MS medium supplemented with IBA (0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with NAA (0.1, 0.5, 1.0 and 3.0 mg L-1). A completely randomized design was used with treatment consisting of 3 replicates with 10 buckets per plot. Seedlings with and without roots were acclimatized in a greenhouse. The best results of multiplication and rooting were obtained in MS medium + 0.1 mg L-1 BAP (80%) and in MS medium + 1.0 mg L-1 IBA (42.6%), respectively. There was no difference in the survival percentage of seedlings in vitro and during ex vitro acclimatization (75%). The micropropagation protocol for production of D. multiflora is effective and can be used for commercial production.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-01
2015-04-30T13:36:04Z
2015-04-30T13:36:04Z
2015-04-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000100011
SOUZA, A. V. de et al. Micropropagação de Dioscorea multiflora Grised. Ciência e Agrotecnologia, Lavras, v. 35, n. 1, p. 92-98, jan./fev. 2011.
http://repositorio.ufla.br/jspui/handle/1/7357
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000100011
http://repositorio.ufla.br/jspui/handle/1/7357
identifier_str_mv SOUZA, A. V. de et al. Micropropagação de Dioscorea multiflora Grised. Ciência e Agrotecnologia, Lavras, v. 35, n. 1, p. 92-98, jan./fev. 2011.
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
publisher.none.fl_str_mv Editora da Universidade Federal de Lavras
dc.source.none.fl_str_mv Ciência e Agrotecnologia v.35 n.1 2011
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
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