Micropropagação de Dioscorea multiflora Grised
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000100011 http://repositorio.ufla.br/jspui/handle/1/7357 |
Resumo: | Dioscorea multiflora is a plant native to southern Brazil that produces diosgenin as a major secondary metabolite, a substance which is used by the pharmaceutical industry for the production of cortisone and substances with contraceptive action. The objective of this work was to optimize the micropropagation protocol of D. multiflora, for the production of seedlings on a commercial scale. Nodal segments subcultured in solid MS medium were transferred for multiplication to MS medium supplemented with BAP (0.01, 0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with 0.1 mg L-1 or 0.5 mg L-1 BAP plus different concentrations of sucrose (2, 4, 6, 8 and 10%). For rooting, the shoots were cultured on MS medium supplemented with IBA (0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with NAA (0.1, 0.5, 1.0 and 3.0 mg L-1). A completely randomized design was used with treatment consisting of 3 replicates with 10 buckets per plot. Seedlings with and without roots were acclimatized in a greenhouse. The best results of multiplication and rooting were obtained in MS medium + 0.1 mg L-1 BAP (80%) and in MS medium + 1.0 mg L-1 IBA (42.6%), respectively. There was no difference in the survival percentage of seedlings in vitro and during ex vitro acclimatization (75%). The micropropagation protocol for production of D. multiflora is effective and can be used for commercial production. |
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Micropropagação de Dioscorea multiflora GrisedMicropropagation of Dioscorea multiflora GrisedDiosgeninaCultivo in vitroAclimataçãoDiosgeninIn vitro cultivationAcclimatizationDioscorea multiflora is a plant native to southern Brazil that produces diosgenin as a major secondary metabolite, a substance which is used by the pharmaceutical industry for the production of cortisone and substances with contraceptive action. The objective of this work was to optimize the micropropagation protocol of D. multiflora, for the production of seedlings on a commercial scale. Nodal segments subcultured in solid MS medium were transferred for multiplication to MS medium supplemented with BAP (0.01, 0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with 0.1 mg L-1 or 0.5 mg L-1 BAP plus different concentrations of sucrose (2, 4, 6, 8 and 10%). For rooting, the shoots were cultured on MS medium supplemented with IBA (0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with NAA (0.1, 0.5, 1.0 and 3.0 mg L-1). A completely randomized design was used with treatment consisting of 3 replicates with 10 buckets per plot. Seedlings with and without roots were acclimatized in a greenhouse. The best results of multiplication and rooting were obtained in MS medium + 0.1 mg L-1 BAP (80%) and in MS medium + 1.0 mg L-1 IBA (42.6%), respectively. There was no difference in the survival percentage of seedlings in vitro and during ex vitro acclimatization (75%). The micropropagation protocol for production of D. multiflora is effective and can be used for commercial production.Dioscorea multiflora uma planta nativa do Sul do Brasil produz a diosgenina como metabólito secundário majoritário, uma substância potencialmente usada pela indústria farmacêutica para a produção de cortisona e substâncias com ação contraceptiva. Objetivou-se, neste trabalho otimizar o protocolo de micropropagação de D. multiflora, visando a produção de mudas em escala comercial. Segmentos nodais subcultivados em meio MS sólido foram transferidos para multiplicação em meio MS suplementado com BAP (0,01; 0,1; 0,5; 1,0 e 3,0 mg L-1)e meio MS suplementado com 0,1 mg L-1 ou 0,5 mg L-1 de BAP acrescido de diferentes concentrações de sacarose (2, 4, 6, 8 e 10%). Para o enraizamento, as brotações foram cultivadas em meio MS suplementado com AIB (0,1; 0,5; 1,0 e 3,0 mg L-1) e meio MS suplementado com ANA (0,1; 0,5; 1,0 e 3,0 mg L-1). Os experimentos in vitro foram instalados em delineamento experimental inteiramente casualizado e cada tratamento constituiu-se de 3 repetições e 10 cubetas/parcela. Plântulas com e sem raízes foram aclimatizadas em casa de vegetação. Melhores resultados de multiplicação e enraizamento foram obtidos em meio MS + 0,1 mg L-1 de BAP (80%) e em meio MS + 1,0 mg L-1 de AIB (42,6%), respectivamente. Não houve diferença quanto à porcentagem de sobrevivência das plântulas in vitro e ex vitro durante a aclimatização (75%). O protocolo de micropropagação para D. Multiflora é efetivo e pode ser usado para a produção em escala comercial.Editora da Universidade Federal de Lavras2011-02-012015-04-30T13:36:04Z2015-04-30T13:36:04Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000100011SOUZA, A. V. de et al. Micropropagação de Dioscorea multiflora Grised. Ciência e Agrotecnologia, Lavras, v. 35, n. 1, p. 92-98, jan./fev. 2011.http://repositorio.ufla.br/jspui/handle/1/7357Ciência e Agrotecnologia v.35 n.1 2011reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLASouza, Ana Valéria deBertoni, Bianca WalériaFrança, Suzelei de CastroPereira, Ana Maria Soaresporinfo:eu-repo/semantics/openAccess2016-07-21T18:04:34Zoai:localhost:1/7357Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-07-21T18:04:34Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Micropropagação de Dioscorea multiflora Grised Micropropagation of Dioscorea multiflora Grised |
title |
Micropropagação de Dioscorea multiflora Grised |
spellingShingle |
Micropropagação de Dioscorea multiflora Grised Souza, Ana Valéria de Diosgenina Cultivo in vitro Aclimatação Diosgenin In vitro cultivation Acclimatization |
title_short |
Micropropagação de Dioscorea multiflora Grised |
title_full |
Micropropagação de Dioscorea multiflora Grised |
title_fullStr |
Micropropagação de Dioscorea multiflora Grised |
title_full_unstemmed |
Micropropagação de Dioscorea multiflora Grised |
title_sort |
Micropropagação de Dioscorea multiflora Grised |
author |
Souza, Ana Valéria de |
author_facet |
Souza, Ana Valéria de Bertoni, Bianca Waléria França, Suzelei de Castro Pereira, Ana Maria Soares |
author_role |
author |
author2 |
Bertoni, Bianca Waléria França, Suzelei de Castro Pereira, Ana Maria Soares |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Souza, Ana Valéria de Bertoni, Bianca Waléria França, Suzelei de Castro Pereira, Ana Maria Soares |
dc.subject.por.fl_str_mv |
Diosgenina Cultivo in vitro Aclimatação Diosgenin In vitro cultivation Acclimatization |
topic |
Diosgenina Cultivo in vitro Aclimatação Diosgenin In vitro cultivation Acclimatization |
description |
Dioscorea multiflora is a plant native to southern Brazil that produces diosgenin as a major secondary metabolite, a substance which is used by the pharmaceutical industry for the production of cortisone and substances with contraceptive action. The objective of this work was to optimize the micropropagation protocol of D. multiflora, for the production of seedlings on a commercial scale. Nodal segments subcultured in solid MS medium were transferred for multiplication to MS medium supplemented with BAP (0.01, 0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with 0.1 mg L-1 or 0.5 mg L-1 BAP plus different concentrations of sucrose (2, 4, 6, 8 and 10%). For rooting, the shoots were cultured on MS medium supplemented with IBA (0.1, 0.5, 1.0 and 3.0 mg L-1) and MS medium supplemented with NAA (0.1, 0.5, 1.0 and 3.0 mg L-1). A completely randomized design was used with treatment consisting of 3 replicates with 10 buckets per plot. Seedlings with and without roots were acclimatized in a greenhouse. The best results of multiplication and rooting were obtained in MS medium + 0.1 mg L-1 BAP (80%) and in MS medium + 1.0 mg L-1 IBA (42.6%), respectively. There was no difference in the survival percentage of seedlings in vitro and during ex vitro acclimatization (75%). The micropropagation protocol for production of D. multiflora is effective and can be used for commercial production. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-02-01 2015-04-30T13:36:04Z 2015-04-30T13:36:04Z 2015-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000100011 SOUZA, A. V. de et al. Micropropagação de Dioscorea multiflora Grised. Ciência e Agrotecnologia, Lavras, v. 35, n. 1, p. 92-98, jan./fev. 2011. http://repositorio.ufla.br/jspui/handle/1/7357 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542011000100011 http://repositorio.ufla.br/jspui/handle/1/7357 |
identifier_str_mv |
SOUZA, A. V. de et al. Micropropagação de Dioscorea multiflora Grised. Ciência e Agrotecnologia, Lavras, v. 35, n. 1, p. 92-98, jan./fev. 2011. |
dc.language.iso.fl_str_mv |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.35 n.1 2011 reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
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Universidade Federal de Lavras (UFLA) |
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UFLA |
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UFLA |
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Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
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Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
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nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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