Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/49355 |
Resumo: | Species of the Digitalis genus, commonly known as ‘Foxglove', are the main source of cardiotonic heterosides (CHs) of the cardenolide type, responsible for much of their pharmacological properties and therapeutic applications. The cardenolide glucoevatromonoside (GEV), produced in Digitalis mariana ssp. heywoodii has great medicinal value for effectively inhibiting the acyclovir-resistant Herpes simplex type 1 (HSV-1) virus, demonstrating anticancer activity in vitro. This paper aimed to evaluate the vegetative growth and accumulation of total cardenolides and glucoevatromonoside (GEV) in cultivated plants of Digitalis mariana ssp. heywoodii, in pots under different colored screens and a hydroponic system under omission of macro and micronutrients. For this purpose, a GEV dosage method by HPLC-DAD was first developed and validated, according to the criteria of the ICH guide - International Conference on Harmonization (ICH, 1996) and ANVISA Resolution RE nº 166 of July 24th, 2017 (BRASIL, 2017). Two agronomic experiments were installed. The first consisted of Digitalis mariana ssp. heywoodii seedlings cultivated under blue, red, and black photo converter screens and full sun. The second experiment consisted of seedlings cultivated in a hydroponic system, employing the missing element technique, with the individual omission of macro and micronutrients from the Hogland and Arnon (1950) solution. The complete Hogland and Arnon (1950) solution was used as the control. Growth parameters, morphology, protein accumulation, photosynthetic pigments, cardenolides, and GEV were analyzed. The differences between the groups evaluated were considered statistically significant according to the Tukey test (5%). The blue screen provided the highest amount of leaf dry matter (12.69 g), root growth (54.00 cm), leaf area (46.97 cm2), protein content (0.118 mg mL-1), and chlorophyll a (1.88 mg g-1). The blue and red screens showed no significant difference at the level of 5% significance (2.29% and 2.33%, respectively) for total cardenolides. The highest levels of GEV were found only in plants cultivated under the blue screen (26.0 μg/100g of plant material). The omission of nitrogen (N), phosphorus (P), sulfur (S), and manganese (Mn) did not affect the growth of the aerial part of the plants. Boron (B) and magnesium (Mg) deficiency caused a greater accumulation of total protein (0.066 and 0.062 mg mL-1, respectively). However, sulfur (S) deficiency caused a decrease of 51.9% compared to the full treatment. The nutrient limitation impacted the accumulation of total cardenolides in the following order: Cu = Fe = Mg = Ca > Mn = B = Zn > S = K = P = N = full. The chromatographic method developed and validated was successfully used in the quantitative analysis of GEV. Different light spectra and availability of macro and micronutrients affect vegetative growth, production of photosynthesizing pigments, proteins and total cardenolide, and GEV levels in Digitalis mariana ssp. heywoodii. |
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Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeosCultivation of Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz under the omission of nutrients in a hydroponic system and colored screens: analytical validation, cardenolide dosage, and vegetative growthHeterosídeos cardiotônicosCardenolídeo glicoevatromonosídeoQualidade espectral de luzElementos faltantesDedaleiraCrescimento vegetativoMissing elementsSpectral quality of lightCardiotonic heterosidesCardenolide glucoevatromonosideVegetative growthFoxgloveFisiologia de Plantas CultivadasSpecies of the Digitalis genus, commonly known as ‘Foxglove', are the main source of cardiotonic heterosides (CHs) of the cardenolide type, responsible for much of their pharmacological properties and therapeutic applications. The cardenolide glucoevatromonoside (GEV), produced in Digitalis mariana ssp. heywoodii has great medicinal value for effectively inhibiting the acyclovir-resistant Herpes simplex type 1 (HSV-1) virus, demonstrating anticancer activity in vitro. This paper aimed to evaluate the vegetative growth and accumulation of total cardenolides and glucoevatromonoside (GEV) in cultivated plants of Digitalis mariana ssp. heywoodii, in pots under different colored screens and a hydroponic system under omission of macro and micronutrients. For this purpose, a GEV dosage method by HPLC-DAD was first developed and validated, according to the criteria of the ICH guide - International Conference on Harmonization (ICH, 1996) and ANVISA Resolution RE nº 166 of July 24th, 2017 (BRASIL, 2017). Two agronomic experiments were installed. The first consisted of Digitalis mariana ssp. heywoodii seedlings cultivated under blue, red, and black photo converter screens and full sun. The second experiment consisted of seedlings cultivated in a hydroponic system, employing the missing element technique, with the individual omission of macro and micronutrients from the Hogland and Arnon (1950) solution. The complete Hogland and Arnon (1950) solution was used as the control. Growth parameters, morphology, protein accumulation, photosynthetic pigments, cardenolides, and GEV were analyzed. The differences between the groups evaluated were considered statistically significant according to the Tukey test (5%). The blue screen provided the highest amount of leaf dry matter (12.69 g), root growth (54.00 cm), leaf area (46.97 cm2), protein content (0.118 mg mL-1), and chlorophyll a (1.88 mg g-1). The blue and red screens showed no significant difference at the level of 5% significance (2.29% and 2.33%, respectively) for total cardenolides. The highest levels of GEV were found only in plants cultivated under the blue screen (26.0 μg/100g of plant material). The omission of nitrogen (N), phosphorus (P), sulfur (S), and manganese (Mn) did not affect the growth of the aerial part of the plants. Boron (B) and magnesium (Mg) deficiency caused a greater accumulation of total protein (0.066 and 0.062 mg mL-1, respectively). However, sulfur (S) deficiency caused a decrease of 51.9% compared to the full treatment. The nutrient limitation impacted the accumulation of total cardenolides in the following order: Cu = Fe = Mg = Ca > Mn = B = Zn > S = K = P = N = full. The chromatographic method developed and validated was successfully used in the quantitative analysis of GEV. Different light spectra and availability of macro and micronutrients affect vegetative growth, production of photosynthesizing pigments, proteins and total cardenolide, and GEV levels in Digitalis mariana ssp. heywoodii.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Espécies do gênero Digitalis, popularmente conhecidas como ‘Dedaleira", são a principal fonte de heterosídeos cardiotônicos (HCs) do tipo cardenolídeos, responsáveis por grande parte de suas propriedades farmacológicas e aplicações terapêuticas. O cardenolídeo glicoevatromonosídeo (GEV), produzido em Digitalis mariana ssp. heywoodii, possui grande valor medicinal por inibir efetivamente o vírus da Herpes simplex tipo 1 (VHS-1), aciclovir-resistente e demonstrou atividade anticancerígena in vitro. Objetivou-se avaliar o crescimento vegetativo e acúmulo de cardenolídeos totais e do glicoevatromonosídeo (GEV), em plantas cultivadas de Digitalis mariana ssp. heywoodii, em vasos sob diferentes malhas coloridas e em sistema hidropônico sob omissão de macro e micronutrientes. Para isto, primeiramente foi desenvolvido e validado um método de doseamento de GEV por CLAE-DAD, conforme critérios do guia ICH - International Conference on Harmonisation (ICH, 1996) e da Resolução RE no 166, de 24 de julho de 2017 da ANVISA (BRASIL, 2017). Foram instalados dois experimentos agronômicos: no primeiro, mudas de D. mariana ssp. heywoodii cultivadas sob malhas fotoconversoras nas colorações azul, vermelha e preta e a pleno-sol. No segundo experimento, mudas foram cultivadas em sistema hidropônico, empregando a técnica do elemento faltante, com omissão individual dos macros e micronutrientes da solução de Hoagland e Arnon (1950). A solução completa de Hoagland e Arnon (1950) foi usada como controle. Parâmetros de crescimento, morfologia, acúmulo de proteínas, pigmentos fotossintéticos, cardenolídeos e GEV foram analisados. As diferenças entre os grupos avaliados foram consideradas estatisticamente significativas de acordo com Tukey (5%). A tela de cor azul proporcionou a maior quantidade de matéria seca de folha (12,69 g), crescimento de raiz (54,00 cm), área foliar (46,97 cm2), teores de proteína (0,118 mg mL-1), clorofila a (1,88 mg g-1). Em relação aos cardenolídeos totais, as telas azul e vermelha não apresentaram diferença significativa ao nível de 5% de significância (2,29% e 2,33%, respectivamente). Os maiores teores de GEV foram encontrados apenas em plantas cultivadas sob tela azul (26,0 μg/ 100g de material vegetal). A omissão de nitrogênio (N), fósforo (P), enxofre (S) e manganês (Mn) não afetou o crescimento da parte aérea das plantas. A deficiência de boro (B) e magnésio (Mg) causou maior acúmulo de proteínas totais (0,066 e 0,062 mg mL-1, respectivamente). Entretanto a deficiência de enxofre (S) causou uma queda de 51,9% em relação ao tratamento completo. A limitação de nutrientes impactou o acúmulo de cardenolídeos totais na seguinte ordem Cu = Fe = Mg = Ca > Mn = B = Zn > S = K = P = N = completo. O método cromatográfico desenvolvido e validado foi empregado com sucesso nas análises quantitativas de GEV. Diferentes espectros luminosos e disponibilidade de macro e micronutrientes influenciam no crescimento vegetativo, produção de pigmentos fotossintetizantes, proteínas e teores de cardenolídeos totais e GEVem Digitalis mariana ssp. heywoodii.Universidade Federal de LavrasPrograma de Pós-Graduação em Plantas Medicinais Aromáticas e CondimentaresUFLAbrasilDepartamento de AgriculturaBertolucci, Suzan Kelly VilelaPinto, José Eduardo Brasil PereiraPádua, Rodrigo Maia deFigueiredo, Felipe CamposChibli, Lucas ApolinárioMarafeli, Érica Alves Marques2022-02-16T21:37:13Z2022-02-16T21:37:13Z2022-02-162021-12-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfMARAFELI, E. A. M. Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos. 2021. 174 p. Tese (Doutorado em Plantas Medicinais, Aromáticas e Condimentares) – Universidade Federal de Lavras, Lavras, 2022.http://repositorio.ufla.br/jspui/handle/1/49355porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2023-04-12T13:00:43Zoai:localhost:1/49355Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-04-12T13:00:43Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos Cultivation of Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz under the omission of nutrients in a hydroponic system and colored screens: analytical validation, cardenolide dosage, and vegetative growth |
title |
Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos |
spellingShingle |
Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos Marafeli, Érica Alves Marques Heterosídeos cardiotônicos Cardenolídeo glicoevatromonosídeo Qualidade espectral de luz Elementos faltantes Dedaleira Crescimento vegetativo Missing elements Spectral quality of light Cardiotonic heterosides Cardenolide glucoevatromonoside Vegetative growth Foxglove Fisiologia de Plantas Cultivadas |
title_short |
Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos |
title_full |
Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos |
title_fullStr |
Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos |
title_full_unstemmed |
Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos |
title_sort |
Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos |
author |
Marafeli, Érica Alves Marques |
author_facet |
Marafeli, Érica Alves Marques |
author_role |
author |
dc.contributor.none.fl_str_mv |
Bertolucci, Suzan Kelly Vilela Pinto, José Eduardo Brasil Pereira Pádua, Rodrigo Maia de Figueiredo, Felipe Campos Chibli, Lucas Apolinário |
dc.contributor.author.fl_str_mv |
Marafeli, Érica Alves Marques |
dc.subject.por.fl_str_mv |
Heterosídeos cardiotônicos Cardenolídeo glicoevatromonosídeo Qualidade espectral de luz Elementos faltantes Dedaleira Crescimento vegetativo Missing elements Spectral quality of light Cardiotonic heterosides Cardenolide glucoevatromonoside Vegetative growth Foxglove Fisiologia de Plantas Cultivadas |
topic |
Heterosídeos cardiotônicos Cardenolídeo glicoevatromonosídeo Qualidade espectral de luz Elementos faltantes Dedaleira Crescimento vegetativo Missing elements Spectral quality of light Cardiotonic heterosides Cardenolide glucoevatromonoside Vegetative growth Foxglove Fisiologia de Plantas Cultivadas |
description |
Species of the Digitalis genus, commonly known as ‘Foxglove', are the main source of cardiotonic heterosides (CHs) of the cardenolide type, responsible for much of their pharmacological properties and therapeutic applications. The cardenolide glucoevatromonoside (GEV), produced in Digitalis mariana ssp. heywoodii has great medicinal value for effectively inhibiting the acyclovir-resistant Herpes simplex type 1 (HSV-1) virus, demonstrating anticancer activity in vitro. This paper aimed to evaluate the vegetative growth and accumulation of total cardenolides and glucoevatromonoside (GEV) in cultivated plants of Digitalis mariana ssp. heywoodii, in pots under different colored screens and a hydroponic system under omission of macro and micronutrients. For this purpose, a GEV dosage method by HPLC-DAD was first developed and validated, according to the criteria of the ICH guide - International Conference on Harmonization (ICH, 1996) and ANVISA Resolution RE nº 166 of July 24th, 2017 (BRASIL, 2017). Two agronomic experiments were installed. The first consisted of Digitalis mariana ssp. heywoodii seedlings cultivated under blue, red, and black photo converter screens and full sun. The second experiment consisted of seedlings cultivated in a hydroponic system, employing the missing element technique, with the individual omission of macro and micronutrients from the Hogland and Arnon (1950) solution. The complete Hogland and Arnon (1950) solution was used as the control. Growth parameters, morphology, protein accumulation, photosynthetic pigments, cardenolides, and GEV were analyzed. The differences between the groups evaluated were considered statistically significant according to the Tukey test (5%). The blue screen provided the highest amount of leaf dry matter (12.69 g), root growth (54.00 cm), leaf area (46.97 cm2), protein content (0.118 mg mL-1), and chlorophyll a (1.88 mg g-1). The blue and red screens showed no significant difference at the level of 5% significance (2.29% and 2.33%, respectively) for total cardenolides. The highest levels of GEV were found only in plants cultivated under the blue screen (26.0 μg/100g of plant material). The omission of nitrogen (N), phosphorus (P), sulfur (S), and manganese (Mn) did not affect the growth of the aerial part of the plants. Boron (B) and magnesium (Mg) deficiency caused a greater accumulation of total protein (0.066 and 0.062 mg mL-1, respectively). However, sulfur (S) deficiency caused a decrease of 51.9% compared to the full treatment. The nutrient limitation impacted the accumulation of total cardenolides in the following order: Cu = Fe = Mg = Ca > Mn = B = Zn > S = K = P = N = full. The chromatographic method developed and validated was successfully used in the quantitative analysis of GEV. Different light spectra and availability of macro and micronutrients affect vegetative growth, production of photosynthesizing pigments, proteins and total cardenolide, and GEV levels in Digitalis mariana ssp. heywoodii. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-03 2022-02-16T21:37:13Z 2022-02-16T21:37:13Z 2022-02-16 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
MARAFELI, E. A. M. Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos. 2021. 174 p. Tese (Doutorado em Plantas Medicinais, Aromáticas e Condimentares) – Universidade Federal de Lavras, Lavras, 2022. http://repositorio.ufla.br/jspui/handle/1/49355 |
identifier_str_mv |
MARAFELI, E. A. M. Cultivo de Digitalis mariana Boiss. ssp. heywoodii (P. Silva and M. Silva) Hinz sob omissão de nutrientes em sistema hidropônico e sob malhas coloridas: crescimento vegetativo e doseamento de cardenolídeos. 2021. 174 p. Tese (Doutorado em Plantas Medicinais, Aromáticas e Condimentares) – Universidade Federal de Lavras, Lavras, 2022. |
url |
http://repositorio.ufla.br/jspui/handle/1/49355 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Plantas Medicinais Aromáticas e Condimentares UFLA brasil Departamento de Agricultura |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Plantas Medicinais Aromáticas e Condimentares UFLA brasil Departamento de Agricultura |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
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UFLA |
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UFLA |
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Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
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Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
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nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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