Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300010 http://repositorio.ufla.br/jspui/handle/1/6070 |
Resumo: | The prerequisite to obtain success in the plants genetic transformation, and also for the fast multiplication of the modified genotype through the micropropagation, it is a efficient regeneration protocol. The objective of this work was to study the expression of the morphogenetic potential of thin fragments of apple tree leaves, and to optimize a regeneration protocol seeking futures works of genetic transformation. The completely randomized experimental design was used, in factorial outline 3 x 2 x 6, with three cultivate of apple tree (Galaxy, Maxigala and Mastergala), two explants types (thin fragments of leaves cut in the transversal and longitudinal direction), and six concentrations of thidiazuron (TDZ) in the culture medium (0; 4.54; 9.08; 13.62; 18.16 and 22.7 µM), totaling 36 treatments. The MS medium salts and vitamins were added of myo-inositol (100 mg.L-1), sucrose (30 g.L-1), agar (6 g.L-1) and 1.6 µM naphthaleneacetic acid (NAA). Flasks with capacity for 150 mL with 6 mL of culture medium were used. The explants were obtained of plants in vitro cultivated, in multiplication phase, 45 days after the inoculation, and they were constituted of fine fragments of the medium part of leaves, cut in the tranversal direction (length from 8 to 10 mm) or longitudinal (length from 10 to 12 mm), both with approximate width of 1 mm. The regeneration percentage, the intensity of callus formation and the number of shoots formed by explant were evaluated by the 45 days of cultivation. Starting from the obtained results it was ended that the expression of the morphogenetic potential of the thin fragments of leaf cut in the transversal direction is higher than those cut in the longitudinal direction, and its cultivation in culture medium with 4.54 µM of TDZ, propitiates, simultaneously, high regeneration percentage, great number of shoots formation and smaller callus intensity. |
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Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhasIn vitro morphogenesis of shoots of apple tree (Malus domestica Borkh.) Starting from thin fragments of leavesCultura de tecidosRegeneração de brotosPlantas - Reguladores de crescimentoThidiazuronTipo de explanteTissue cultureShoots regenerationPlants - Growth regulatorsExplant typeThe prerequisite to obtain success in the plants genetic transformation, and also for the fast multiplication of the modified genotype through the micropropagation, it is a efficient regeneration protocol. The objective of this work was to study the expression of the morphogenetic potential of thin fragments of apple tree leaves, and to optimize a regeneration protocol seeking futures works of genetic transformation. The completely randomized experimental design was used, in factorial outline 3 x 2 x 6, with three cultivate of apple tree (Galaxy, Maxigala and Mastergala), two explants types (thin fragments of leaves cut in the transversal and longitudinal direction), and six concentrations of thidiazuron (TDZ) in the culture medium (0; 4.54; 9.08; 13.62; 18.16 and 22.7 µM), totaling 36 treatments. The MS medium salts and vitamins were added of myo-inositol (100 mg.L-1), sucrose (30 g.L-1), agar (6 g.L-1) and 1.6 µM naphthaleneacetic acid (NAA). Flasks with capacity for 150 mL with 6 mL of culture medium were used. The explants were obtained of plants in vitro cultivated, in multiplication phase, 45 days after the inoculation, and they were constituted of fine fragments of the medium part of leaves, cut in the tranversal direction (length from 8 to 10 mm) or longitudinal (length from 10 to 12 mm), both with approximate width of 1 mm. The regeneration percentage, the intensity of callus formation and the number of shoots formed by explant were evaluated by the 45 days of cultivation. Starting from the obtained results it was ended that the expression of the morphogenetic potential of the thin fragments of leaf cut in the transversal direction is higher than those cut in the longitudinal direction, and its cultivation in culture medium with 4.54 µM of TDZ, propitiates, simultaneously, high regeneration percentage, great number of shoots formation and smaller callus intensity.O pré-requisito para se obter sucesso na transformação genética de plantas, e também para a multiplicação rápida do genótipo modificado pela micropropagação, é um protocolo de regeneração eficiente. Objetivou-se com este trabalho estudar a expressão do potencial morfogenético de fragmentos delgados de folhas de macieira e otimizar um protocolo de regeneração visando futuros trabalhos de transformação genética. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 3 x 2 x 6, com três cultivares de macieira (Galaxy, Maxigala e Mastergala), dois tipos de explantes (fragmentos finos de folhas cortados no sentido transversal e longitudinal), e seis concentrações de thidiazuron (TDZ) no meio de cultura (0; 4,54; 9,08; 13,62; 18,16 e 22,7 µM), totalizando 36 tratamentos. Os sais e vitaminas do meio MS foram acrescidos de mio-inositol (100 mg.L-1), sacarose (30 g.L-1), ágar (6 g.L-1) e adicionados de 1,6 µM ácido naftalenoacético (ANA). Foram utilizados frascos com capacidade para 150 mL com 6 mL de meio de cultura. Os explantes foram obtidos de plantas cultivadas in vitro, em fase de multiplicação, 45 dias após a repicagem, e se constituíram de finos fragmentos da parte mediana de folhas, cortados no sentido transversal (comprimento de 8 a 10 mm) ou longitudinal (comprimento de 10 a 12 mm), ambos com largura aproximada de 1 mm. A porcentagem de regeneração, a intensidade de formação de calo e o número de brotos por explante foram avaliados aos 45 dias de cultivo. A partir dos resultados obtidos concluiu-se que a expressão do potencial morfogenético dos fragmentos delgados de folhas cortados no sentido transversal é maior do que o daqueles cortados no sentido longitudinal, e o seu cultivo em meio de cultura com 4,54 µM de TDZ, propicia, simultaneamente, elevada porcentagem de regeneração, maior número de brotos e menor intensidade de calo.Editora da Universidade Federal de Lavras2005-06-012015-04-30T13:33:29Z2015-04-30T13:33:29Z2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300010ERIG, A. C.; SCHUCH, M. W. Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas. Ciência e Agrotecnologia, Lavras, v. 29, n. 3, p. 575-581, maio/jun. 2005.http://repositorio.ufla.br/jspui/handle/1/6070Ciência e Agrotecnologia v.29 n.3 2005reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAErig, Alan CristianoSchuch, Márcia Wulffporinfo:eu-repo/semantics/openAccess2016-07-22T13:48:45Zoai:localhost:1/6070Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-07-22T13:48:45Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas In vitro morphogenesis of shoots of apple tree (Malus domestica Borkh.) Starting from thin fragments of leaves |
title |
Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas |
spellingShingle |
Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas Erig, Alan Cristiano Cultura de tecidos Regeneração de brotos Plantas - Reguladores de crescimento Thidiazuron Tipo de explante Tissue culture Shoots regeneration Plants - Growth regulators Explant type |
title_short |
Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas |
title_full |
Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas |
title_fullStr |
Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas |
title_full_unstemmed |
Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas |
title_sort |
Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas |
author |
Erig, Alan Cristiano |
author_facet |
Erig, Alan Cristiano Schuch, Márcia Wulff |
author_role |
author |
author2 |
Schuch, Márcia Wulff |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Erig, Alan Cristiano Schuch, Márcia Wulff |
dc.subject.por.fl_str_mv |
Cultura de tecidos Regeneração de brotos Plantas - Reguladores de crescimento Thidiazuron Tipo de explante Tissue culture Shoots regeneration Plants - Growth regulators Explant type |
topic |
Cultura de tecidos Regeneração de brotos Plantas - Reguladores de crescimento Thidiazuron Tipo de explante Tissue culture Shoots regeneration Plants - Growth regulators Explant type |
description |
The prerequisite to obtain success in the plants genetic transformation, and also for the fast multiplication of the modified genotype through the micropropagation, it is a efficient regeneration protocol. The objective of this work was to study the expression of the morphogenetic potential of thin fragments of apple tree leaves, and to optimize a regeneration protocol seeking futures works of genetic transformation. The completely randomized experimental design was used, in factorial outline 3 x 2 x 6, with three cultivate of apple tree (Galaxy, Maxigala and Mastergala), two explants types (thin fragments of leaves cut in the transversal and longitudinal direction), and six concentrations of thidiazuron (TDZ) in the culture medium (0; 4.54; 9.08; 13.62; 18.16 and 22.7 µM), totaling 36 treatments. The MS medium salts and vitamins were added of myo-inositol (100 mg.L-1), sucrose (30 g.L-1), agar (6 g.L-1) and 1.6 µM naphthaleneacetic acid (NAA). Flasks with capacity for 150 mL with 6 mL of culture medium were used. The explants were obtained of plants in vitro cultivated, in multiplication phase, 45 days after the inoculation, and they were constituted of fine fragments of the medium part of leaves, cut in the tranversal direction (length from 8 to 10 mm) or longitudinal (length from 10 to 12 mm), both with approximate width of 1 mm. The regeneration percentage, the intensity of callus formation and the number of shoots formed by explant were evaluated by the 45 days of cultivation. Starting from the obtained results it was ended that the expression of the morphogenetic potential of the thin fragments of leaf cut in the transversal direction is higher than those cut in the longitudinal direction, and its cultivation in culture medium with 4.54 µM of TDZ, propitiates, simultaneously, high regeneration percentage, great number of shoots formation and smaller callus intensity. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-06-01 2015-04-30T13:33:29Z 2015-04-30T13:33:29Z 2015-04-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300010 ERIG, A. C.; SCHUCH, M. W. Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas. Ciência e Agrotecnologia, Lavras, v. 29, n. 3, p. 575-581, maio/jun. 2005. http://repositorio.ufla.br/jspui/handle/1/6070 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000300010 http://repositorio.ufla.br/jspui/handle/1/6070 |
identifier_str_mv |
ERIG, A. C.; SCHUCH, M. W. Morfogênese in vitro de brotos de macieira (Malus domestica Borkh.) a partir de fragmentos delgados de folhas. Ciência e Agrotecnologia, Lavras, v. 29, n. 3, p. 575-581, maio/jun. 2005. |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia v.29 n.3 2005 reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1784550132147027968 |