Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/50423 |
Resumo: | Background: Micropropagation, an in vitro vegetative propagation technique using small propagules is one of the main applications of plant tissue culture. It can be used to clone specific plants with desired traits and reduce the cost of plant propagation. In this study, we developed a protocol for micropropagation of Eucalyptus microcorys F.Muell using a selected mature tree, in which we tested various combinations of different culture media and evaluated the use of biodegradable polyester-based microvessels during the adventitious rooting and acclimatisation phases. Methods: Epicormic shoots were used as an explant source. After the in vitro explant establishment and multiplication, we tested 8 combinations of BAP, NAA and IBA in the elongation phase. Three types of microvessels were tested in the adventitious rooting phase and acclimatisation of the microcuttings. Results: Epicormic shoots had an establishment percentage of 40.6% and a total of 820 explants were generated by the 11th subculture, with an average of 12 buds per explant. Best shoot elongation results were achieved with BAP (0.05 mg L-1) + NAA (1 mg L-1) and BAP (0.05 mg L-1) + NAA (1 mg L-1) + IBA (1 mg L-1) combinations, whereas microvessel types M2 and M3 provided higher rooting and acclimatisation. According to the results of ISSR markers, at the end of 535 days of in vitro cultivation, cloning was successful between acclimatised micro-plantlets and the parent plant. Conclusions: The micropropagation protocol using microvessels was efficient in producing E. microcorys clonal micro-plantlets and is recommended for further studies with this species, and for testing in the micropropagation of other species. |
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Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorysIn vitro culturePlant cloningPlant growth regulatorRooting containerVegetative propagationCultura in vitroPlantas - ClonagemReguladores de crescimento vegetalPropagação vegetativaBackground: Micropropagation, an in vitro vegetative propagation technique using small propagules is one of the main applications of plant tissue culture. It can be used to clone specific plants with desired traits and reduce the cost of plant propagation. In this study, we developed a protocol for micropropagation of Eucalyptus microcorys F.Muell using a selected mature tree, in which we tested various combinations of different culture media and evaluated the use of biodegradable polyester-based microvessels during the adventitious rooting and acclimatisation phases. Methods: Epicormic shoots were used as an explant source. After the in vitro explant establishment and multiplication, we tested 8 combinations of BAP, NAA and IBA in the elongation phase. Three types of microvessels were tested in the adventitious rooting phase and acclimatisation of the microcuttings. Results: Epicormic shoots had an establishment percentage of 40.6% and a total of 820 explants were generated by the 11th subculture, with an average of 12 buds per explant. Best shoot elongation results were achieved with BAP (0.05 mg L-1) + NAA (1 mg L-1) and BAP (0.05 mg L-1) + NAA (1 mg L-1) + IBA (1 mg L-1) combinations, whereas microvessel types M2 and M3 provided higher rooting and acclimatisation. According to the results of ISSR markers, at the end of 535 days of in vitro cultivation, cloning was successful between acclimatised micro-plantlets and the parent plant. Conclusions: The micropropagation protocol using microvessels was efficient in producing E. microcorys clonal micro-plantlets and is recommended for further studies with this species, and for testing in the micropropagation of other species.Scion2022-06-30T22:11:33Z2022-06-30T22:11:33Z2022-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfFARIA, J. C. T. et al. Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys. New Zealand Journal of Forestry Science, [S.I.], v. 52, n. 10, 2022. DOI: https://doi.org/10.33494/nzjfs522022x139x.http://repositorio.ufla.br/jspui/handle/1/50423New Zealand Journal of Forestry Sciencereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessFaria, Júlio Cézar TannureRibeiro-Kumara, CaiusCosta, Rayssa Silva da RochaNieri, Erick MartinsCarvalho, Dulcinéia dePinto, José Eduardo Brasil PereiraSena Neto, Alfredo Rodrigues deBrondani, Gilvano Eblingeng2023-05-26T18:50:30Zoai:localhost:1/50423Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-26T18:50:30Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys |
title |
Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys |
spellingShingle |
Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys Faria, Júlio Cézar Tannure In vitro culture Plant cloning Plant growth regulator Rooting container Vegetative propagation Cultura in vitro Plantas - Clonagem Reguladores de crescimento vegetal Propagação vegetativa |
title_short |
Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys |
title_full |
Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys |
title_fullStr |
Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys |
title_full_unstemmed |
Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys |
title_sort |
Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys |
author |
Faria, Júlio Cézar Tannure |
author_facet |
Faria, Júlio Cézar Tannure Ribeiro-Kumara, Caius Costa, Rayssa Silva da Rocha Nieri, Erick Martins Carvalho, Dulcinéia de Pinto, José Eduardo Brasil Pereira Sena Neto, Alfredo Rodrigues de Brondani, Gilvano Ebling |
author_role |
author |
author2 |
Ribeiro-Kumara, Caius Costa, Rayssa Silva da Rocha Nieri, Erick Martins Carvalho, Dulcinéia de Pinto, José Eduardo Brasil Pereira Sena Neto, Alfredo Rodrigues de Brondani, Gilvano Ebling |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Faria, Júlio Cézar Tannure Ribeiro-Kumara, Caius Costa, Rayssa Silva da Rocha Nieri, Erick Martins Carvalho, Dulcinéia de Pinto, José Eduardo Brasil Pereira Sena Neto, Alfredo Rodrigues de Brondani, Gilvano Ebling |
dc.subject.por.fl_str_mv |
In vitro culture Plant cloning Plant growth regulator Rooting container Vegetative propagation Cultura in vitro Plantas - Clonagem Reguladores de crescimento vegetal Propagação vegetativa |
topic |
In vitro culture Plant cloning Plant growth regulator Rooting container Vegetative propagation Cultura in vitro Plantas - Clonagem Reguladores de crescimento vegetal Propagação vegetativa |
description |
Background: Micropropagation, an in vitro vegetative propagation technique using small propagules is one of the main applications of plant tissue culture. It can be used to clone specific plants with desired traits and reduce the cost of plant propagation. In this study, we developed a protocol for micropropagation of Eucalyptus microcorys F.Muell using a selected mature tree, in which we tested various combinations of different culture media and evaluated the use of biodegradable polyester-based microvessels during the adventitious rooting and acclimatisation phases. Methods: Epicormic shoots were used as an explant source. After the in vitro explant establishment and multiplication, we tested 8 combinations of BAP, NAA and IBA in the elongation phase. Three types of microvessels were tested in the adventitious rooting phase and acclimatisation of the microcuttings. Results: Epicormic shoots had an establishment percentage of 40.6% and a total of 820 explants were generated by the 11th subculture, with an average of 12 buds per explant. Best shoot elongation results were achieved with BAP (0.05 mg L-1) + NAA (1 mg L-1) and BAP (0.05 mg L-1) + NAA (1 mg L-1) + IBA (1 mg L-1) combinations, whereas microvessel types M2 and M3 provided higher rooting and acclimatisation. According to the results of ISSR markers, at the end of 535 days of in vitro cultivation, cloning was successful between acclimatised micro-plantlets and the parent plant. Conclusions: The micropropagation protocol using microvessels was efficient in producing E. microcorys clonal micro-plantlets and is recommended for further studies with this species, and for testing in the micropropagation of other species. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-30T22:11:33Z 2022-06-30T22:11:33Z 2022-04 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FARIA, J. C. T. et al. Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys. New Zealand Journal of Forestry Science, [S.I.], v. 52, n. 10, 2022. DOI: https://doi.org/10.33494/nzjfs522022x139x. http://repositorio.ufla.br/jspui/handle/1/50423 |
identifier_str_mv |
FARIA, J. C. T. et al. Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys. New Zealand Journal of Forestry Science, [S.I.], v. 52, n. 10, 2022. DOI: https://doi.org/10.33494/nzjfs522022x139x. |
url |
http://repositorio.ufla.br/jspui/handle/1/50423 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Scion |
publisher.none.fl_str_mv |
Scion |
dc.source.none.fl_str_mv |
New Zealand Journal of Forestry Science reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835051405082624 |