Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion

Detalhes bibliográficos
Autor(a) principal: Alves, Eduardo
Data de Publicação: 2009
Outros Autores: Leite, Breno, Pascholati, Sérgio Florentino, Ishida, Maria Lúcia, Andersen, Peter Craig
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/28504
Resumo: Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is an important disease of citrus in Brazil. X. fastidiosa is restricted to xylem vessels of plants and knowledge regarding xylem colonization is still limited. Our goal was to verify how this bacterium colonizes and spreads within xylem vessels of sweet orange Citrus sinensis cv. Pêra. Petioles and pieces of leaf blades from naturally infected plant exhibiting characteristic symptoms were prepared for light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and immunogold labeling (IGL). Petioles from healthy plants were used as control. IGL results, using an antibody against wall hemicelluloses, revealed that the pit membrane of vessels was altered. Bacterial cells were observed in the pit between adjacent vessels. Results support the contention that X. fastidiosa produces cellulases to reach adjacent vessels. SEM revealed that colonization of sweet orange started with X. fastidiosa cells attaching to the xylem wall, followed by an increase in the number of bacterial cells, the production of fibrous material, and finally vessel occlusion by biofilm composed of copious amounts of amorphous material, strands and cells. Phenolic materials, hyperplasia and hypertrophy were noticed in leaves with gummy material. Xylem vessels frequently contained an unknown needle-like, crystallized matter blocking the vessel.
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spelling Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusionColonização de pecíolo e folha de Citrus sinensis por Xylella fastidiosa: detalhes da obstrução de vasos do xilemaUltrastructureScanning electron microscopyTransmission electron microscopyImmunolabelUltra-estruturaMicroscopia eletrônica de varreduraMicroscopia eletrônica de transmissãoImunomarcaçãoCitrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is an important disease of citrus in Brazil. X. fastidiosa is restricted to xylem vessels of plants and knowledge regarding xylem colonization is still limited. Our goal was to verify how this bacterium colonizes and spreads within xylem vessels of sweet orange Citrus sinensis cv. Pêra. Petioles and pieces of leaf blades from naturally infected plant exhibiting characteristic symptoms were prepared for light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and immunogold labeling (IGL). Petioles from healthy plants were used as control. IGL results, using an antibody against wall hemicelluloses, revealed that the pit membrane of vessels was altered. Bacterial cells were observed in the pit between adjacent vessels. Results support the contention that X. fastidiosa produces cellulases to reach adjacent vessels. SEM revealed that colonization of sweet orange started with X. fastidiosa cells attaching to the xylem wall, followed by an increase in the number of bacterial cells, the production of fibrous material, and finally vessel occlusion by biofilm composed of copious amounts of amorphous material, strands and cells. Phenolic materials, hyperplasia and hypertrophy were noticed in leaves with gummy material. Xylem vessels frequently contained an unknown needle-like, crystallized matter blocking the vessel.A clorose variegada dos citrus (CVC), causada por uma bactéria restrita ao xilema (Xylella fastidiosa), é uma importante doença de citros no Brasil, entretanto, pouco se sabe sobre a colonização dos vasos do xilema pela bactéria. O objetivo deste trabalho foi estudar como X. fastidiosa invade os vasos adjacentes do xilema e algumas das alterações expressas por plantas de laranja Pêra. Foram coletadas 15 amostras de pecíolos e áreas das folhas de plantas com sintomas característicos da doença, as quais foram preparadas para microscopia de luz, microscopia eletrônica de varredura (MEV) e transmissão (MET) e imunomarcação de polissacarídeos da parede primária. Pecíolos de folhas sadias foram utilizados como controle. Os resultados da imunomarcação para hemicelulose mostraram alterações na integridade da membrana da pontuação. A bactéria foi localizada nas imediações e no interior das pontuações entre dois vasos. Estas observações suportam a hipótese de que X. fastidiosa produz celulases para alcançar os vasos adjacentes do xilema. Imagens de MEV revelaram que a colonização dos vasos do xilema de laranja doce inicia-se com as células da bactéria aderidas às paredes do xilema, seguida pelo aumento no número de células bacterianas, produção de material extracelular e finalmente a obstrução do vaso. Também, nos tecidos das folhas, verificou-se a deposição de goma, compostos fenólicos, hiperplasia e alterações no citoplasma das células da bainha e dos parênquimas paliçádico e esponjoso. Foi também observada presença de material cristalino formado por estruturas em forma de agulhas nos vasos do xilema, freqüentemente causando sua obstrução.Escola Superior de Agricultura "Luiz de Queiroz"2018-01-27T11:51:22Z2018-01-27T11:51:22Z2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfALVES, E. et al. Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion. Scientia Agricola, Piracicaba, v. 66, n. 2, p. 218-224, Mar./Apr. 2009.http://repositorio.ufla.br/jspui/handle/1/28504Scientia Agricolareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessAlves, EduardoLeite, BrenoPascholati, Sérgio FlorentinoIshida, Maria LúciaAndersen, Peter Craigpor2018-01-27T11:51:23Zoai:localhost:1/28504Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-01-27T11:51:23Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion
Colonização de pecíolo e folha de Citrus sinensis por Xylella fastidiosa: detalhes da obstrução de vasos do xilema
title Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion
spellingShingle Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion
Alves, Eduardo
Ultrastructure
Scanning electron microscopy
Transmission electron microscopy
Immunolabel
Ultra-estrutura
Microscopia eletrônica de varredura
Microscopia eletrônica de transmissão
Imunomarcação
title_short Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion
title_full Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion
title_fullStr Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion
title_full_unstemmed Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion
title_sort Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion
author Alves, Eduardo
author_facet Alves, Eduardo
Leite, Breno
Pascholati, Sérgio Florentino
Ishida, Maria Lúcia
Andersen, Peter Craig
author_role author
author2 Leite, Breno
Pascholati, Sérgio Florentino
Ishida, Maria Lúcia
Andersen, Peter Craig
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Alves, Eduardo
Leite, Breno
Pascholati, Sérgio Florentino
Ishida, Maria Lúcia
Andersen, Peter Craig
dc.subject.por.fl_str_mv Ultrastructure
Scanning electron microscopy
Transmission electron microscopy
Immunolabel
Ultra-estrutura
Microscopia eletrônica de varredura
Microscopia eletrônica de transmissão
Imunomarcação
topic Ultrastructure
Scanning electron microscopy
Transmission electron microscopy
Immunolabel
Ultra-estrutura
Microscopia eletrônica de varredura
Microscopia eletrônica de transmissão
Imunomarcação
description Citrus variegated chlorosis (CVC), caused by Xylella fastidiosa, is an important disease of citrus in Brazil. X. fastidiosa is restricted to xylem vessels of plants and knowledge regarding xylem colonization is still limited. Our goal was to verify how this bacterium colonizes and spreads within xylem vessels of sweet orange Citrus sinensis cv. Pêra. Petioles and pieces of leaf blades from naturally infected plant exhibiting characteristic symptoms were prepared for light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and immunogold labeling (IGL). Petioles from healthy plants were used as control. IGL results, using an antibody against wall hemicelluloses, revealed that the pit membrane of vessels was altered. Bacterial cells were observed in the pit between adjacent vessels. Results support the contention that X. fastidiosa produces cellulases to reach adjacent vessels. SEM revealed that colonization of sweet orange started with X. fastidiosa cells attaching to the xylem wall, followed by an increase in the number of bacterial cells, the production of fibrous material, and finally vessel occlusion by biofilm composed of copious amounts of amorphous material, strands and cells. Phenolic materials, hyperplasia and hypertrophy were noticed in leaves with gummy material. Xylem vessels frequently contained an unknown needle-like, crystallized matter blocking the vessel.
publishDate 2009
dc.date.none.fl_str_mv 2009
2018-01-27T11:51:22Z
2018-01-27T11:51:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ALVES, E. et al. Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion. Scientia Agricola, Piracicaba, v. 66, n. 2, p. 218-224, Mar./Apr. 2009.
http://repositorio.ufla.br/jspui/handle/1/28504
identifier_str_mv ALVES, E. et al. Citrus sinensis leaf petiole and blade colonization by Xylella fastidiosa: details of xylem vessel occlusion. Scientia Agricola, Piracicaba, v. 66, n. 2, p. 218-224, Mar./Apr. 2009.
url http://repositorio.ufla.br/jspui/handle/1/28504
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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