Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/49591 |
Resumo: | The root-knot nematode, Meloidogyne javanica, is a harmful pathogen for soybeans. Understanding how the semiochemicals released by the soybean root system affect the second juvenile (J2) of M. javanica can help in the development of new management measures for this pathogen. In the present study, an open environment technique was developed for the study of J2 chemotaxis in soil. Our results indicate that the roots of soybean cv. Monsoy 5917 IPRO release semiochemicals that are attractive (p < 0.05) to juveniles. However, most J2 remained close to the infestation site, apparently without being affected by the semiochemicals. Next, by means of gas chromatography coupled to mass spectrometry (GC-MS), the volatile organic compounds (VOCs) released by the soybean root system were identified. A total of 13 compounds were detected in soybean emissions, of wich six: ethyl acetate, ethyl benzoate, etanol, tetradecane, 2-pentylfuran and 3-pentanone, were acquired and tested in vitro chemotaxis assays. In addition to these VOCs, the compounds vanillin and salicylic acid were also tested and these were not identified in the GC-MS of soybeans and were used for comparison routes beacuse they appear in the literature as attractive to the J2 of Meloidogyne sp. The J2s were not attracted to any of the six VOCs detected in soybean root, but were highly attracted to the compounds vanillin and salicylic acid. Finally, using the chemotaxis technique developed here, the compounds vanillin and salicylic acid were tested in pots containing a mixture of sand and soil. In the first test, in the region of application of the salicylic acid compound, an average of 6x more J2 was recovered. However, in the second assay none of the compounds attracted J2. |
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Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de sojaDistance call: chemotaxis of Meloidogyne javanica to volatile organic compounds emitted by soybean rootNematoide das galhasMeloidogyne javanicaAtração e repelênciaAleloquímicosManejo de patógenosRoot-knot nematodeAttraction and repulsionAllelochemicalsPathogen managementFitopatologiaThe root-knot nematode, Meloidogyne javanica, is a harmful pathogen for soybeans. Understanding how the semiochemicals released by the soybean root system affect the second juvenile (J2) of M. javanica can help in the development of new management measures for this pathogen. In the present study, an open environment technique was developed for the study of J2 chemotaxis in soil. Our results indicate that the roots of soybean cv. Monsoy 5917 IPRO release semiochemicals that are attractive (p < 0.05) to juveniles. However, most J2 remained close to the infestation site, apparently without being affected by the semiochemicals. Next, by means of gas chromatography coupled to mass spectrometry (GC-MS), the volatile organic compounds (VOCs) released by the soybean root system were identified. A total of 13 compounds were detected in soybean emissions, of wich six: ethyl acetate, ethyl benzoate, etanol, tetradecane, 2-pentylfuran and 3-pentanone, were acquired and tested in vitro chemotaxis assays. In addition to these VOCs, the compounds vanillin and salicylic acid were also tested and these were not identified in the GC-MS of soybeans and were used for comparison routes beacuse they appear in the literature as attractive to the J2 of Meloidogyne sp. The J2s were not attracted to any of the six VOCs detected in soybean root, but were highly attracted to the compounds vanillin and salicylic acid. Finally, using the chemotaxis technique developed here, the compounds vanillin and salicylic acid were tested in pots containing a mixture of sand and soil. In the first test, in the region of application of the salicylic acid compound, an average of 6x more J2 was recovered. However, in the second assay none of the compounds attracted J2.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)O nematoide de galhas, Meloidogyne javanica, é um patógeno danoso para a cultura da soja. Entender como os semioquímicos liberados pelo sistema radicular da soja afetam o juvenil do segundo estádio (J2) de M. javanica pode auxiliar no desenvolvimento de novas medidas de manejo para este patógeno. No presente estudo, foi desenvolvida uma técnica em ambiente aberto para o estudo da quimiotaxia de J2 em solo. Nossos resultados indicam que as raízes de soja cv. Monsoy 5917 IPRO liberam semioquímicos que são atraentes (p < 0,05) aos juvenis. Contudo, a maioria dos J2 permaneceu próximo ao local de infestação, aparentemente sem sofrer atuação dos semioquímicos. A seguir, por meio da cromatografia gasosa acoplada a espectrometria de massa (GC-MS), identificou-se os compostos orgânicos voláteis (COVs) liberados pelo sistema radicular da soja. Um total de 13 compostos foram detectados nas emissões da soja, dos quais seis: acetato de etila, benzoato de etila, etanol e tetradecano, 2-pentilfurano e 3-pentanona, foram adquiridos e testados em ensaios de quimiotaxia in vitro. Além destes COVs testou-se também os compostos vanilina e ácido salicílico e estes não foram identificados no GC-MS da soja e foram utilizados para vias de comparação por constar na literatura como atraentes aos J2 de Meloidogyne sp. Os J2 não foram atraídos por nenhum dos seis COVs detectados na raiz da soja, mas foram altamente atraídos pelos compostos vanilina e ácido salicílico. Por fim, utilizando-se a técnica de quimiotaxia aqui desenvolvida testou-se os compostos vanilina e ácido salicílico em vasos contendo a mistura de areia e solo. No primeiro ensaio, na região de aplicação do composto ácido salicílico foram recuperados em média 6x mais J2. Entretanto, no segundo ensaio nenhum dos compostos atraiu os J2.Universidade Federal de LavrasPrograma de Pós-Graduação em Agronomia/FitopatologiaUFLAbrasilDepartamento de FitopatologiaCampos, Vicente PauloPedroso, Márcio PozzobonFreire, Eduardo SouzaAndrade-Souza, Larissa Fernanda2022-03-25T20:31:20Z2022-03-25T20:31:20Z2022-03-252022-02-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfANDRADE-SOUZA, L. F. Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja. 2022. 51 p. Dissertação (Mestrado em Agronomia/Fitopatologia) – Universidade Federal de Lavras, Lavras, 2022.http://repositorio.ufla.br/jspui/handle/1/49591porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2023-05-11T19:33:57Zoai:localhost:1/49591Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-11T19:33:57Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja Distance call: chemotaxis of Meloidogyne javanica to volatile organic compounds emitted by soybean root |
title |
Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja |
spellingShingle |
Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja Andrade-Souza, Larissa Fernanda Nematoide das galhas Meloidogyne javanica Atração e repelência Aleloquímicos Manejo de patógenos Root-knot nematode Attraction and repulsion Allelochemicals Pathogen management Fitopatologia |
title_short |
Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja |
title_full |
Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja |
title_fullStr |
Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja |
title_full_unstemmed |
Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja |
title_sort |
Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja |
author |
Andrade-Souza, Larissa Fernanda |
author_facet |
Andrade-Souza, Larissa Fernanda |
author_role |
author |
dc.contributor.none.fl_str_mv |
Campos, Vicente Paulo Pedroso, Márcio Pozzobon Freire, Eduardo Souza |
dc.contributor.author.fl_str_mv |
Andrade-Souza, Larissa Fernanda |
dc.subject.por.fl_str_mv |
Nematoide das galhas Meloidogyne javanica Atração e repelência Aleloquímicos Manejo de patógenos Root-knot nematode Attraction and repulsion Allelochemicals Pathogen management Fitopatologia |
topic |
Nematoide das galhas Meloidogyne javanica Atração e repelência Aleloquímicos Manejo de patógenos Root-knot nematode Attraction and repulsion Allelochemicals Pathogen management Fitopatologia |
description |
The root-knot nematode, Meloidogyne javanica, is a harmful pathogen for soybeans. Understanding how the semiochemicals released by the soybean root system affect the second juvenile (J2) of M. javanica can help in the development of new management measures for this pathogen. In the present study, an open environment technique was developed for the study of J2 chemotaxis in soil. Our results indicate that the roots of soybean cv. Monsoy 5917 IPRO release semiochemicals that are attractive (p < 0.05) to juveniles. However, most J2 remained close to the infestation site, apparently without being affected by the semiochemicals. Next, by means of gas chromatography coupled to mass spectrometry (GC-MS), the volatile organic compounds (VOCs) released by the soybean root system were identified. A total of 13 compounds were detected in soybean emissions, of wich six: ethyl acetate, ethyl benzoate, etanol, tetradecane, 2-pentylfuran and 3-pentanone, were acquired and tested in vitro chemotaxis assays. In addition to these VOCs, the compounds vanillin and salicylic acid were also tested and these were not identified in the GC-MS of soybeans and were used for comparison routes beacuse they appear in the literature as attractive to the J2 of Meloidogyne sp. The J2s were not attracted to any of the six VOCs detected in soybean root, but were highly attracted to the compounds vanillin and salicylic acid. Finally, using the chemotaxis technique developed here, the compounds vanillin and salicylic acid were tested in pots containing a mixture of sand and soil. In the first test, in the region of application of the salicylic acid compound, an average of 6x more J2 was recovered. However, in the second assay none of the compounds attracted J2. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-25T20:31:20Z 2022-03-25T20:31:20Z 2022-03-25 2022-02-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ANDRADE-SOUZA, L. F. Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja. 2022. 51 p. Dissertação (Mestrado em Agronomia/Fitopatologia) – Universidade Federal de Lavras, Lavras, 2022. http://repositorio.ufla.br/jspui/handle/1/49591 |
identifier_str_mv |
ANDRADE-SOUZA, L. F. Chamada à distância: auimiotaxia de Meloidogyne javanica a compostos orgânicos voláteis emitidos por raiz de soja. 2022. 51 p. Dissertação (Mestrado em Agronomia/Fitopatologia) – Universidade Federal de Lavras, Lavras, 2022. |
url |
http://repositorio.ufla.br/jspui/handle/1/49591 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Agronomia/Fitopatologia UFLA brasil Departamento de Fitopatologia |
publisher.none.fl_str_mv |
Universidade Federal de Lavras Programa de Pós-Graduação em Agronomia/Fitopatologia UFLA brasil Departamento de Fitopatologia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
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Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835144485076992 |