Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn

Detalhes bibliográficos
Autor(a) principal: Stein, Vanessa Cristina
Data de Publicação: 2010
Outros Autores: Paiva, Renato, Vargas, Daiane Peixoto, Soares, Fernanda Pereira, Alves, Eduardo, Nogueira, Gabriela Ferreira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/28505
Resumo: Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, nodal segments and leaves were disinfected and inoculated in test tubes containing MS medium with 3% sucrose and 4.5µM 2.4-D, except for leaf callogenesis, where 9µM of this auxin was used, and for the callogenesis of anthers and ovaries, where the culture medium was enriched with 0.25% activated charcoal and 90µM PVP. After 45 days in culture medium, the anther, ovary, leaf and nodal segment calli were fixed in Karnovisky and prepared for visualization by scanning and transmission electron microscopy. Ultrastructural differences were observed among the callus cells of anthers, ovaries, segments and leaves. There was no evidence of somatic embryo formation in the anther, leaf and nodal segment calli, in spite of some embryogenic characteristics in the cells. The ovary calli, with indications of embryo formation, seem to be the most responsive explant source for embryogenesis.
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spelling Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. PennAnálise ultraestrutural de calos de ingazeiro (Inga vera Willd. subsp. Affinis (DC.) T.D. Penn.)AnthersOvariesNodal segmentsAnterasOváriosSegmentos foliaresSubcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, nodal segments and leaves were disinfected and inoculated in test tubes containing MS medium with 3% sucrose and 4.5µM 2.4-D, except for leaf callogenesis, where 9µM of this auxin was used, and for the callogenesis of anthers and ovaries, where the culture medium was enriched with 0.25% activated charcoal and 90µM PVP. After 45 days in culture medium, the anther, ovary, leaf and nodal segment calli were fixed in Karnovisky and prepared for visualization by scanning and transmission electron microscopy. Ultrastructural differences were observed among the callus cells of anthers, ovaries, segments and leaves. There was no evidence of somatic embryo formation in the anther, leaf and nodal segment calli, in spite of some embryogenic characteristics in the cells. The ovary calli, with indications of embryo formation, seem to be the most responsive explant source for embryogenesis.A compreensão da organogênese e embriogênese de plantas, nos estágios iniciais de desenvolvimento das células, requer a observação das mudanças subcelulares. No entanto, a caracterização citológica durante o desenvolvimento desses estágios não tem sido realizada com frequência na cultura de tecidos. Portanto, o objetivo deste trabalho foi caracterizar as diferenças ultraestruturais relacionadas a calogênese de anteras, ovários, folha e segmentos nodais de Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Para tanto, botões florais, segmentos nodais e folhas foram desinfestados e inoculados em tubos de ensaio contendo meio MS com 3% de sacarose e 4,5 µM de 2,4-D, exceto na calogênese de folhas, em que foram utilizados 9 µM dessa auxina, e na calogênese de anteras e ovários, cujo meio de cultura foi acrescido de 0,25% de carvão ativado e 90 µM de PVP. Após 45 dias em meio de cultura, os calos de anteras, ovários, folhas e segmentos nodais foram fixados em Karnovisky e preparados para a visualização em microscopia eletrônica de varredura e microscopia eletrônica de transmissão. Assim, foram observadas diferenças ultraestruturais entre as células dos calos provenientes de anteras, ovários, segmentos e folhas. Os calos de anteras, folhas e segmentos nodais não apresentaram evidências de formação de embriões somáticos, apesar de suas células possuírem algumas características embriogênicas. No entanto, os calos de ovários parecem ser a fonte de explante mais responsiva à embriogênese, mostrando indícios de formação de embriões.Sociedade de Investigações Florestais2018-01-27T12:24:12Z2018-01-27T12:24:12Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSTEIN, V. C. et al. Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Revista Árvore, Viçosa, MG, v. 34, n. 5, p. 789-796, 2010.http://repositorio.ufla.br/jspui/handle/1/28505Revista Árvorereponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessStein, Vanessa CristinaPaiva, RenatoVargas, Daiane PeixotoSoares, Fernanda PereiraAlves, EduardoNogueira, Gabriela Ferreirapor2018-01-27T12:24:12Zoai:localhost:1/28505Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-01-27T12:24:12Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn
Análise ultraestrutural de calos de ingazeiro (Inga vera Willd. subsp. Affinis (DC.) T.D. Penn.)
title Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn
spellingShingle Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn
Stein, Vanessa Cristina
Anthers
Ovaries
Nodal segments
Anteras
Ovários
Segmentos foliares
title_short Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn
title_full Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn
title_fullStr Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn
title_full_unstemmed Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn
title_sort Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn
author Stein, Vanessa Cristina
author_facet Stein, Vanessa Cristina
Paiva, Renato
Vargas, Daiane Peixoto
Soares, Fernanda Pereira
Alves, Eduardo
Nogueira, Gabriela Ferreira
author_role author
author2 Paiva, Renato
Vargas, Daiane Peixoto
Soares, Fernanda Pereira
Alves, Eduardo
Nogueira, Gabriela Ferreira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Stein, Vanessa Cristina
Paiva, Renato
Vargas, Daiane Peixoto
Soares, Fernanda Pereira
Alves, Eduardo
Nogueira, Gabriela Ferreira
dc.subject.por.fl_str_mv Anthers
Ovaries
Nodal segments
Anteras
Ovários
Segmentos foliares
topic Anthers
Ovaries
Nodal segments
Anteras
Ovários
Segmentos foliares
description Subcellular changes are relevant to understand plant organogenesis and embryogenesis in the early stages of cell development. The cytology during cell development in tissue culture is however still poorly characterized. This study aimed to characterize the ultrastructural differences related to callogenesis of anthers, ovaries, leaf and nodal segments of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Flower buds, nodal segments and leaves were disinfected and inoculated in test tubes containing MS medium with 3% sucrose and 4.5µM 2.4-D, except for leaf callogenesis, where 9µM of this auxin was used, and for the callogenesis of anthers and ovaries, where the culture medium was enriched with 0.25% activated charcoal and 90µM PVP. After 45 days in culture medium, the anther, ovary, leaf and nodal segment calli were fixed in Karnovisky and prepared for visualization by scanning and transmission electron microscopy. Ultrastructural differences were observed among the callus cells of anthers, ovaries, segments and leaves. There was no evidence of somatic embryo formation in the anther, leaf and nodal segment calli, in spite of some embryogenic characteristics in the cells. The ovary calli, with indications of embryo formation, seem to be the most responsive explant source for embryogenesis.
publishDate 2010
dc.date.none.fl_str_mv 2010
2018-01-27T12:24:12Z
2018-01-27T12:24:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv STEIN, V. C. et al. Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Revista Árvore, Viçosa, MG, v. 34, n. 5, p. 789-796, 2010.
http://repositorio.ufla.br/jspui/handle/1/28505
identifier_str_mv STEIN, V. C. et al. Ultrastructural calli analysis of Inga vera Willd. subsp. Affinis (DC.) T.D. Penn. Revista Árvore, Viçosa, MG, v. 34, n. 5, p. 789-796, 2010.
url http://repositorio.ufla.br/jspui/handle/1/28505
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Investigações Florestais
publisher.none.fl_str_mv Sociedade de Investigações Florestais
dc.source.none.fl_str_mv Revista Árvore
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815439060831830016