Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle

Detalhes bibliográficos
Autor(a) principal: Carvalho, Elisa Barbosa de
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/11853
Resumo: Residual feed intake (RFI) has been extensively used to evaluate feed efficiency, however the molecular underlying differences in this trait remains unclear. In order to characterize mechanisms driving differences on energy metabolism of beef cattle skeletal muscle and liver tissue proteome was analyzed. From a group of 120 Nellore young bulls identified for RFI, cattle with the highest (n=9) and lowest (n=9) values were selected for protein abundance studies. Samples were collected immediately after slaughter and subjected to two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry to identify differentially abundant proteins associated with RFI values. Two differentially abundant spots were identified in skeletal muscle of high RFI group (Alpha actin 1 e 14-3-3 protein epsilon), one was identified in muscle of low RFI group (Heat shock protein beta-1) and one was identified in liver tissue of low RFI group (10-formyltetrahydrofolate dehydrogenase) (P<0.05). These data indicates that genetic selection for RFI tends to cause changes in skeletal muscle and liver protein profile, suggesting that differences in low and high RFI cattle may be due skeletal muscle protein turnover and liver energy metabolism.
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spelling Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattlePerfil proteômico do tecido muscular esquelético e tecido hepático de bovinos Nelore com alto e baixo consumo alimentar residualBeef cattle - NutritionMass spectrometryResidual feed intakeBovino de corte - NutriçãoEspectrometria de massaConsumo alimentar residualNutrição e Alimentação AnimalResidual feed intake (RFI) has been extensively used to evaluate feed efficiency, however the molecular underlying differences in this trait remains unclear. In order to characterize mechanisms driving differences on energy metabolism of beef cattle skeletal muscle and liver tissue proteome was analyzed. From a group of 120 Nellore young bulls identified for RFI, cattle with the highest (n=9) and lowest (n=9) values were selected for protein abundance studies. Samples were collected immediately after slaughter and subjected to two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry to identify differentially abundant proteins associated with RFI values. Two differentially abundant spots were identified in skeletal muscle of high RFI group (Alpha actin 1 e 14-3-3 protein epsilon), one was identified in muscle of low RFI group (Heat shock protein beta-1) and one was identified in liver tissue of low RFI group (10-formyltetrahydrofolate dehydrogenase) (P<0.05). These data indicates that genetic selection for RFI tends to cause changes in skeletal muscle and liver protein profile, suggesting that differences in low and high RFI cattle may be due skeletal muscle protein turnover and liver energy metabolism.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)O consumo alimentar residual (CAR) tem sido amplamente utilizado para avaliar a eficiência alimentar, no entanto a base molecular desta característica permanece obscura. A fim de caracterizar os mecanismos responsáveis por diferenças no metabolismo energético de gado de corte, o proteoma do músculo esquelético e tecido hepático foi analisado. A partir de um grupo de 120 machos Nelore avaliados para CAR, os animais com os maiores (n= 9) e menores (n = 9) valores foram selecionados para estudos de abundância de proteína. As amostras foram coletadas imediatamente após o abate e submetidas a eletroforese bidimensional em gel (2-DE) seguida de espectrometria de massas para identificação de proteínas diferencialmente abundantes associadas com os valores de CAR. Dois spots diferencialmente abundantes foram identificados no músculo esquelético do grupo de alto CAR (Alfa actin - 1 e 14-3-3 epsilon), um foi identificado no músculo do grupo de baixo CAR (Heat Shock Protein Beta-1) e um foi identificado no fígado do grupo de baixo CAR (10-Formyltetrahydrofolate dehydrogenase) (p≤0,05). Estes dados indicam que a seleção genética para CAR tende a causar alterações no perfil de proteínas do músculo esquelético e fígado, sugerindo que as diferenças entre animais com baixo e alto CAR podem ser ocasionadas por turnover proteico do músculo esquelético e metabolismo energético do fígado.Universidade Federal de LavrasPrograma de Pós-Graduação em ZootecniaUFLAbrasilDepartamento de ZootecniaGionbelli, Mateus PiesDuarte, Marcio de SouzaLadeira, Márcio MachadoCarvalho, Elisa Barbosa de2016-10-04T13:21:53Z2016-10-04T13:21:53Z2016-10-032016-08-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfCARVALHO, E. B. de. Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle. 2016. 65 p. Dissertação (Mestrado em Zootecnia)-Universidade Federal de Lavras, Lavras, 2016.http://repositorio.ufla.br/jspui/handle/1/11853enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLA2021-11-16T12:40:41Zoai:localhost:1/11853Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-11-16T12:40:41Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle
Perfil proteômico do tecido muscular esquelético e tecido hepático de bovinos Nelore com alto e baixo consumo alimentar residual
title Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle
spellingShingle Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle
Carvalho, Elisa Barbosa de
Beef cattle - Nutrition
Mass spectrometry
Residual feed intake
Bovino de corte - Nutrição
Espectrometria de massa
Consumo alimentar residual
Nutrição e Alimentação Animal
title_short Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle
title_full Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle
title_fullStr Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle
title_full_unstemmed Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle
title_sort Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle
author Carvalho, Elisa Barbosa de
author_facet Carvalho, Elisa Barbosa de
author_role author
dc.contributor.none.fl_str_mv Gionbelli, Mateus Pies
Duarte, Marcio de Souza
Ladeira, Márcio Machado
dc.contributor.author.fl_str_mv Carvalho, Elisa Barbosa de
dc.subject.por.fl_str_mv Beef cattle - Nutrition
Mass spectrometry
Residual feed intake
Bovino de corte - Nutrição
Espectrometria de massa
Consumo alimentar residual
Nutrição e Alimentação Animal
topic Beef cattle - Nutrition
Mass spectrometry
Residual feed intake
Bovino de corte - Nutrição
Espectrometria de massa
Consumo alimentar residual
Nutrição e Alimentação Animal
description Residual feed intake (RFI) has been extensively used to evaluate feed efficiency, however the molecular underlying differences in this trait remains unclear. In order to characterize mechanisms driving differences on energy metabolism of beef cattle skeletal muscle and liver tissue proteome was analyzed. From a group of 120 Nellore young bulls identified for RFI, cattle with the highest (n=9) and lowest (n=9) values were selected for protein abundance studies. Samples were collected immediately after slaughter and subjected to two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry to identify differentially abundant proteins associated with RFI values. Two differentially abundant spots were identified in skeletal muscle of high RFI group (Alpha actin 1 e 14-3-3 protein epsilon), one was identified in muscle of low RFI group (Heat shock protein beta-1) and one was identified in liver tissue of low RFI group (10-formyltetrahydrofolate dehydrogenase) (P<0.05). These data indicates that genetic selection for RFI tends to cause changes in skeletal muscle and liver protein profile, suggesting that differences in low and high RFI cattle may be due skeletal muscle protein turnover and liver energy metabolism.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-04T13:21:53Z
2016-10-04T13:21:53Z
2016-10-03
2016-08-16
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv CARVALHO, E. B. de. Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle. 2016. 65 p. Dissertação (Mestrado em Zootecnia)-Universidade Federal de Lavras, Lavras, 2016.
http://repositorio.ufla.br/jspui/handle/1/11853
identifier_str_mv CARVALHO, E. B. de. Proteomic profile os skeletal muscle and liver tissue of high and low residual feed intake identified Nellore cattle. 2016. 65 p. Dissertação (Mestrado em Zootecnia)-Universidade Federal de Lavras, Lavras, 2016.
url http://repositorio.ufla.br/jspui/handle/1/11853
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Zootecnia
UFLA
brasil
Departamento de Zootecnia
publisher.none.fl_str_mv Universidade Federal de Lavras
Programa de Pós-Graduação em Zootecnia
UFLA
brasil
Departamento de Zootecnia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
_version_ 1815439206811435008

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