Evolution of aerenchyma formation in a maize breeding program

Detalhes bibliográficos
Autor(a) principal: Campos, Nádia Alves
Data de Publicação: 2016
Outros Autores: Alves, José Donizeti, Souza, Kamila Rezende Dázio de, Porto, Brenda Neves, Magalhães, Marcelo Murad, Silva, Glacy Jaqueline da, Paiva, Luciano Vilela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/31926
Resumo: Approximately 28 million hectares of intermittently flooded land with agricultural potential in Brazil could be used for rice crop rotation to increase production. Understanding the flood tolerance mechanisms in "Saracura" is of paramount importance for maize and other crops. Thus, we evaluated the expression levels of the superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, and catalase antioxidant genes and cell wall loosening (XET) enzyme in maize roots under flooding as well as associated expression with the initiation of aerenchyma formation, which is the most important plant adaptation to flooding. We collected data on "Saracura" at the beginning of the breeding program (cycle 1), a time considered more sensitive to flooding, and during the last selection cycle (cycle 18), a time considered more resistant to flooding. Maize plants were flooded for 12 and 24h, and roots were collected for anatomic (aerenchyma density in the root cortex) and gene expression analyses by qRT-PCR. Our results showed that there was a proportional graded increase in aerenchyma formed in the root cortex with increased flooding time that was most pronounced during selection cycle 18. Antioxidant enzyme gene expression levels at both time points were similar: high expression just after germination, decreased expression after 12h, and increased expression after 24h of flooding. Aerenchyma formation was detectable after 24h of flooding, which coincided with the highest antioxidant enzyme gene expression levels. These results indicate that the correlation between antioxidant enzyme activities and flooding can act as a molecular flag to improve flood tolerance in "Saracura". Our results are important to understand the induction of aerenchyma formation and flooding tolerance in "Saracura"
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spelling Evolution of aerenchyma formation in a maize breeding programFloodingGene expressionAntioxidant system enzymesAerenchyma formationMaize - BreedingInundaçãoExpressão genéticaEnzimas do sistema antioxidanteFormação de aerênquimaMilho - Melhoramento genéticoApproximately 28 million hectares of intermittently flooded land with agricultural potential in Brazil could be used for rice crop rotation to increase production. Understanding the flood tolerance mechanisms in "Saracura" is of paramount importance for maize and other crops. Thus, we evaluated the expression levels of the superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, and catalase antioxidant genes and cell wall loosening (XET) enzyme in maize roots under flooding as well as associated expression with the initiation of aerenchyma formation, which is the most important plant adaptation to flooding. We collected data on "Saracura" at the beginning of the breeding program (cycle 1), a time considered more sensitive to flooding, and during the last selection cycle (cycle 18), a time considered more resistant to flooding. Maize plants were flooded for 12 and 24h, and roots were collected for anatomic (aerenchyma density in the root cortex) and gene expression analyses by qRT-PCR. Our results showed that there was a proportional graded increase in aerenchyma formed in the root cortex with increased flooding time that was most pronounced during selection cycle 18. Antioxidant enzyme gene expression levels at both time points were similar: high expression just after germination, decreased expression after 12h, and increased expression after 24h of flooding. Aerenchyma formation was detectable after 24h of flooding, which coincided with the highest antioxidant enzyme gene expression levels. These results indicate that the correlation between antioxidant enzyme activities and flooding can act as a molecular flag to improve flood tolerance in "Saracura". Our results are important to understand the induction of aerenchyma formation and flooding tolerance in "Saracura"Southern Cross Publishing2018-11-23T14:51:52Z2018-11-23T14:51:52Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCAMPOS, N. A. et al. Evolution of aerenchyma formation in a maize breeding program. Plant Omics Journal, [S. l.], v. 9, n. 1, p. 19-25, 2016.http://repositorio.ufla.br/jspui/handle/1/31926Plant Omics Journalreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessCampos, Nádia AlvesAlves, José DonizetiSouza, Kamila Rezende Dázio dePorto, Brenda NevesMagalhães, Marcelo MuradSilva, Glacy Jaqueline daPaiva, Luciano Vilelaeng2018-11-23T14:51:52Zoai:localhost:1/31926Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-11-23T14:51:52Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Evolution of aerenchyma formation in a maize breeding program
title Evolution of aerenchyma formation in a maize breeding program
spellingShingle Evolution of aerenchyma formation in a maize breeding program
Campos, Nádia Alves
Flooding
Gene expression
Antioxidant system enzymes
Aerenchyma formation
Maize - Breeding
Inundação
Expressão genética
Enzimas do sistema antioxidante
Formação de aerênquima
Milho - Melhoramento genético
title_short Evolution of aerenchyma formation in a maize breeding program
title_full Evolution of aerenchyma formation in a maize breeding program
title_fullStr Evolution of aerenchyma formation in a maize breeding program
title_full_unstemmed Evolution of aerenchyma formation in a maize breeding program
title_sort Evolution of aerenchyma formation in a maize breeding program
author Campos, Nádia Alves
author_facet Campos, Nádia Alves
Alves, José Donizeti
Souza, Kamila Rezende Dázio de
Porto, Brenda Neves
Magalhães, Marcelo Murad
Silva, Glacy Jaqueline da
Paiva, Luciano Vilela
author_role author
author2 Alves, José Donizeti
Souza, Kamila Rezende Dázio de
Porto, Brenda Neves
Magalhães, Marcelo Murad
Silva, Glacy Jaqueline da
Paiva, Luciano Vilela
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Campos, Nádia Alves
Alves, José Donizeti
Souza, Kamila Rezende Dázio de
Porto, Brenda Neves
Magalhães, Marcelo Murad
Silva, Glacy Jaqueline da
Paiva, Luciano Vilela
dc.subject.por.fl_str_mv Flooding
Gene expression
Antioxidant system enzymes
Aerenchyma formation
Maize - Breeding
Inundação
Expressão genética
Enzimas do sistema antioxidante
Formação de aerênquima
Milho - Melhoramento genético
topic Flooding
Gene expression
Antioxidant system enzymes
Aerenchyma formation
Maize - Breeding
Inundação
Expressão genética
Enzimas do sistema antioxidante
Formação de aerênquima
Milho - Melhoramento genético
description Approximately 28 million hectares of intermittently flooded land with agricultural potential in Brazil could be used for rice crop rotation to increase production. Understanding the flood tolerance mechanisms in "Saracura" is of paramount importance for maize and other crops. Thus, we evaluated the expression levels of the superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, and catalase antioxidant genes and cell wall loosening (XET) enzyme in maize roots under flooding as well as associated expression with the initiation of aerenchyma formation, which is the most important plant adaptation to flooding. We collected data on "Saracura" at the beginning of the breeding program (cycle 1), a time considered more sensitive to flooding, and during the last selection cycle (cycle 18), a time considered more resistant to flooding. Maize plants were flooded for 12 and 24h, and roots were collected for anatomic (aerenchyma density in the root cortex) and gene expression analyses by qRT-PCR. Our results showed that there was a proportional graded increase in aerenchyma formed in the root cortex with increased flooding time that was most pronounced during selection cycle 18. Antioxidant enzyme gene expression levels at both time points were similar: high expression just after germination, decreased expression after 12h, and increased expression after 24h of flooding. Aerenchyma formation was detectable after 24h of flooding, which coincided with the highest antioxidant enzyme gene expression levels. These results indicate that the correlation between antioxidant enzyme activities and flooding can act as a molecular flag to improve flood tolerance in "Saracura". Our results are important to understand the induction of aerenchyma formation and flooding tolerance in "Saracura"
publishDate 2016
dc.date.none.fl_str_mv 2016
2018-11-23T14:51:52Z
2018-11-23T14:51:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv CAMPOS, N. A. et al. Evolution of aerenchyma formation in a maize breeding program. Plant Omics Journal, [S. l.], v. 9, n. 1, p. 19-25, 2016.
http://repositorio.ufla.br/jspui/handle/1/31926
identifier_str_mv CAMPOS, N. A. et al. Evolution of aerenchyma formation in a maize breeding program. Plant Omics Journal, [S. l.], v. 9, n. 1, p. 19-25, 2016.
url http://repositorio.ufla.br/jspui/handle/1/31926
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Southern Cross Publishing
publisher.none.fl_str_mv Southern Cross Publishing
dc.source.none.fl_str_mv Plant Omics Journal
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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