Evolution of aerenchyma formation in a maize breeding program
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/31926 |
Resumo: | Approximately 28 million hectares of intermittently flooded land with agricultural potential in Brazil could be used for rice crop rotation to increase production. Understanding the flood tolerance mechanisms in "Saracura" is of paramount importance for maize and other crops. Thus, we evaluated the expression levels of the superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, and catalase antioxidant genes and cell wall loosening (XET) enzyme in maize roots under flooding as well as associated expression with the initiation of aerenchyma formation, which is the most important plant adaptation to flooding. We collected data on "Saracura" at the beginning of the breeding program (cycle 1), a time considered more sensitive to flooding, and during the last selection cycle (cycle 18), a time considered more resistant to flooding. Maize plants were flooded for 12 and 24h, and roots were collected for anatomic (aerenchyma density in the root cortex) and gene expression analyses by qRT-PCR. Our results showed that there was a proportional graded increase in aerenchyma formed in the root cortex with increased flooding time that was most pronounced during selection cycle 18. Antioxidant enzyme gene expression levels at both time points were similar: high expression just after germination, decreased expression after 12h, and increased expression after 24h of flooding. Aerenchyma formation was detectable after 24h of flooding, which coincided with the highest antioxidant enzyme gene expression levels. These results indicate that the correlation between antioxidant enzyme activities and flooding can act as a molecular flag to improve flood tolerance in "Saracura". Our results are important to understand the induction of aerenchyma formation and flooding tolerance in "Saracura" |
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Evolution of aerenchyma formation in a maize breeding programFloodingGene expressionAntioxidant system enzymesAerenchyma formationMaize - BreedingInundaçãoExpressão genéticaEnzimas do sistema antioxidanteFormação de aerênquimaMilho - Melhoramento genéticoApproximately 28 million hectares of intermittently flooded land with agricultural potential in Brazil could be used for rice crop rotation to increase production. Understanding the flood tolerance mechanisms in "Saracura" is of paramount importance for maize and other crops. Thus, we evaluated the expression levels of the superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, and catalase antioxidant genes and cell wall loosening (XET) enzyme in maize roots under flooding as well as associated expression with the initiation of aerenchyma formation, which is the most important plant adaptation to flooding. We collected data on "Saracura" at the beginning of the breeding program (cycle 1), a time considered more sensitive to flooding, and during the last selection cycle (cycle 18), a time considered more resistant to flooding. Maize plants were flooded for 12 and 24h, and roots were collected for anatomic (aerenchyma density in the root cortex) and gene expression analyses by qRT-PCR. Our results showed that there was a proportional graded increase in aerenchyma formed in the root cortex with increased flooding time that was most pronounced during selection cycle 18. Antioxidant enzyme gene expression levels at both time points were similar: high expression just after germination, decreased expression after 12h, and increased expression after 24h of flooding. Aerenchyma formation was detectable after 24h of flooding, which coincided with the highest antioxidant enzyme gene expression levels. These results indicate that the correlation between antioxidant enzyme activities and flooding can act as a molecular flag to improve flood tolerance in "Saracura". Our results are important to understand the induction of aerenchyma formation and flooding tolerance in "Saracura"Southern Cross Publishing2018-11-23T14:51:52Z2018-11-23T14:51:52Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfCAMPOS, N. A. et al. Evolution of aerenchyma formation in a maize breeding program. Plant Omics Journal, [S. l.], v. 9, n. 1, p. 19-25, 2016.http://repositorio.ufla.br/jspui/handle/1/31926Plant Omics Journalreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessCampos, Nádia AlvesAlves, José DonizetiSouza, Kamila Rezende Dázio dePorto, Brenda NevesMagalhães, Marcelo MuradSilva, Glacy Jaqueline daPaiva, Luciano Vilelaeng2018-11-23T14:51:52Zoai:localhost:1/31926Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-11-23T14:51:52Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Evolution of aerenchyma formation in a maize breeding program |
title |
Evolution of aerenchyma formation in a maize breeding program |
spellingShingle |
Evolution of aerenchyma formation in a maize breeding program Campos, Nádia Alves Flooding Gene expression Antioxidant system enzymes Aerenchyma formation Maize - Breeding Inundação Expressão genética Enzimas do sistema antioxidante Formação de aerênquima Milho - Melhoramento genético |
title_short |
Evolution of aerenchyma formation in a maize breeding program |
title_full |
Evolution of aerenchyma formation in a maize breeding program |
title_fullStr |
Evolution of aerenchyma formation in a maize breeding program |
title_full_unstemmed |
Evolution of aerenchyma formation in a maize breeding program |
title_sort |
Evolution of aerenchyma formation in a maize breeding program |
author |
Campos, Nádia Alves |
author_facet |
Campos, Nádia Alves Alves, José Donizeti Souza, Kamila Rezende Dázio de Porto, Brenda Neves Magalhães, Marcelo Murad Silva, Glacy Jaqueline da Paiva, Luciano Vilela |
author_role |
author |
author2 |
Alves, José Donizeti Souza, Kamila Rezende Dázio de Porto, Brenda Neves Magalhães, Marcelo Murad Silva, Glacy Jaqueline da Paiva, Luciano Vilela |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Campos, Nádia Alves Alves, José Donizeti Souza, Kamila Rezende Dázio de Porto, Brenda Neves Magalhães, Marcelo Murad Silva, Glacy Jaqueline da Paiva, Luciano Vilela |
dc.subject.por.fl_str_mv |
Flooding Gene expression Antioxidant system enzymes Aerenchyma formation Maize - Breeding Inundação Expressão genética Enzimas do sistema antioxidante Formação de aerênquima Milho - Melhoramento genético |
topic |
Flooding Gene expression Antioxidant system enzymes Aerenchyma formation Maize - Breeding Inundação Expressão genética Enzimas do sistema antioxidante Formação de aerênquima Milho - Melhoramento genético |
description |
Approximately 28 million hectares of intermittently flooded land with agricultural potential in Brazil could be used for rice crop rotation to increase production. Understanding the flood tolerance mechanisms in "Saracura" is of paramount importance for maize and other crops. Thus, we evaluated the expression levels of the superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, and catalase antioxidant genes and cell wall loosening (XET) enzyme in maize roots under flooding as well as associated expression with the initiation of aerenchyma formation, which is the most important plant adaptation to flooding. We collected data on "Saracura" at the beginning of the breeding program (cycle 1), a time considered more sensitive to flooding, and during the last selection cycle (cycle 18), a time considered more resistant to flooding. Maize plants were flooded for 12 and 24h, and roots were collected for anatomic (aerenchyma density in the root cortex) and gene expression analyses by qRT-PCR. Our results showed that there was a proportional graded increase in aerenchyma formed in the root cortex with increased flooding time that was most pronounced during selection cycle 18. Antioxidant enzyme gene expression levels at both time points were similar: high expression just after germination, decreased expression after 12h, and increased expression after 24h of flooding. Aerenchyma formation was detectable after 24h of flooding, which coincided with the highest antioxidant enzyme gene expression levels. These results indicate that the correlation between antioxidant enzyme activities and flooding can act as a molecular flag to improve flood tolerance in "Saracura". Our results are important to understand the induction of aerenchyma formation and flooding tolerance in "Saracura" |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2018-11-23T14:51:52Z 2018-11-23T14:51:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
CAMPOS, N. A. et al. Evolution of aerenchyma formation in a maize breeding program. Plant Omics Journal, [S. l.], v. 9, n. 1, p. 19-25, 2016. http://repositorio.ufla.br/jspui/handle/1/31926 |
identifier_str_mv |
CAMPOS, N. A. et al. Evolution of aerenchyma formation in a maize breeding program. Plant Omics Journal, [S. l.], v. 9, n. 1, p. 19-25, 2016. |
url |
http://repositorio.ufla.br/jspui/handle/1/31926 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Southern Cross Publishing |
publisher.none.fl_str_mv |
Southern Cross Publishing |
dc.source.none.fl_str_mv |
Plant Omics Journal reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1815439374815330304 |