Gene expression analysis associated with tissue-specific promoters in Musa spp.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/55326 |
Resumo: | The study of promoters has become essential to elucidate genetic regulation and allow new genetic transformation strategies through plant biotechnology. The challenge is to discover and validate promoters that can regulate gene transcription spatially and/or temporally. The goal of this work was to validate genes associated with tissue-specific promoters of bananas obtained from in silico sequences and selected from the DATAMusa databank. Gene expression was quantified using RT-qPCR from different tissues: leaves, flowers, roots, unripe pulp, ripe pulp, unripe peels, and ripe peels of two different genetic groups: Prata-Anã (PA; group AAB) and Grand Naine (GN; group AAA). After the analysis of the expression of genes associated with the promoters, normalization was performed with the most stable reference genes (TUB and L2) selected using the RefFinder tool. It was determined that five genes were specific or expressed to a greater extent in some tissues than others. The EMB-23 gene was highly expressed in ripe pulp and flowers of GN, EMB-26 in the ripe pulp of GN, EMB-27 in flowers of GN, EMB-28 in roots of PA and ripe pulp and roots of GN, and EMB-31 in roots and flowers of GN and PA, and unripe pulp of GN. The in silico analysis was efficient in the identification of spatial/time-specific genes, thereby decreasing analysis time and cost, making future genetic transformation studies focusing on the application of these tissue-specific promoters possible. |
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Gene expression analysis associated with tissue-specific promoters in Musa spp.BananaRTqPCRPlant biotechnologyGene expressionGenetic transformationThe study of promoters has become essential to elucidate genetic regulation and allow new genetic transformation strategies through plant biotechnology. The challenge is to discover and validate promoters that can regulate gene transcription spatially and/or temporally. The goal of this work was to validate genes associated with tissue-specific promoters of bananas obtained from in silico sequences and selected from the DATAMusa databank. Gene expression was quantified using RT-qPCR from different tissues: leaves, flowers, roots, unripe pulp, ripe pulp, unripe peels, and ripe peels of two different genetic groups: Prata-Anã (PA; group AAB) and Grand Naine (GN; group AAA). After the analysis of the expression of genes associated with the promoters, normalization was performed with the most stable reference genes (TUB and L2) selected using the RefFinder tool. It was determined that five genes were specific or expressed to a greater extent in some tissues than others. The EMB-23 gene was highly expressed in ripe pulp and flowers of GN, EMB-26 in the ripe pulp of GN, EMB-27 in flowers of GN, EMB-28 in roots of PA and ripe pulp and roots of GN, and EMB-31 in roots and flowers of GN and PA, and unripe pulp of GN. The in silico analysis was efficient in the identification of spatial/time-specific genes, thereby decreasing analysis time and cost, making future genetic transformation studies focusing on the application of these tissue-specific promoters possible.Editora da Universidade Estadual de Maringá (EDUEM)2022-10-24T14:38:14Z2022-10-24T14:38:14Z2022-06-29info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLIVRAMENTO, K. G. do et al. Gene expression analysis associated with tissue-specific promoters in Musa spp. Acta Scientiarum-Agronomy, [S.l.], v. 44, n. 1, p. 1-9, 2022. DOI: 10.4025/actasciagron.v44i1.55893.http://repositorio.ufla.br/jspui/handle/1/55326Acta Scientiarum-Agronomyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessLivramento, Kalynka Gabriella doFreitas, Natália ChagasTrindade, Luciene de Oliveira RibeiroTeixeira, Luiz Gustavo da SilvaPaiva, Luciano VilelaBordallo, Patrícia do NascimentoDiniz, Leandro Eugenio Cardamoneeng2023-05-29T11:07:54Zoai:localhost:1/55326Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2023-05-29T11:07:54Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Gene expression analysis associated with tissue-specific promoters in Musa spp. |
title |
Gene expression analysis associated with tissue-specific promoters in Musa spp. |
spellingShingle |
Gene expression analysis associated with tissue-specific promoters in Musa spp. Livramento, Kalynka Gabriella do Banana RTqPCR Plant biotechnology Gene expression Genetic transformation |
title_short |
Gene expression analysis associated with tissue-specific promoters in Musa spp. |
title_full |
Gene expression analysis associated with tissue-specific promoters in Musa spp. |
title_fullStr |
Gene expression analysis associated with tissue-specific promoters in Musa spp. |
title_full_unstemmed |
Gene expression analysis associated with tissue-specific promoters in Musa spp. |
title_sort |
Gene expression analysis associated with tissue-specific promoters in Musa spp. |
author |
Livramento, Kalynka Gabriella do |
author_facet |
Livramento, Kalynka Gabriella do Freitas, Natália Chagas Trindade, Luciene de Oliveira Ribeiro Teixeira, Luiz Gustavo da Silva Paiva, Luciano Vilela Bordallo, Patrícia do Nascimento Diniz, Leandro Eugenio Cardamone |
author_role |
author |
author2 |
Freitas, Natália Chagas Trindade, Luciene de Oliveira Ribeiro Teixeira, Luiz Gustavo da Silva Paiva, Luciano Vilela Bordallo, Patrícia do Nascimento Diniz, Leandro Eugenio Cardamone |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Livramento, Kalynka Gabriella do Freitas, Natália Chagas Trindade, Luciene de Oliveira Ribeiro Teixeira, Luiz Gustavo da Silva Paiva, Luciano Vilela Bordallo, Patrícia do Nascimento Diniz, Leandro Eugenio Cardamone |
dc.subject.por.fl_str_mv |
Banana RTqPCR Plant biotechnology Gene expression Genetic transformation |
topic |
Banana RTqPCR Plant biotechnology Gene expression Genetic transformation |
description |
The study of promoters has become essential to elucidate genetic regulation and allow new genetic transformation strategies through plant biotechnology. The challenge is to discover and validate promoters that can regulate gene transcription spatially and/or temporally. The goal of this work was to validate genes associated with tissue-specific promoters of bananas obtained from in silico sequences and selected from the DATAMusa databank. Gene expression was quantified using RT-qPCR from different tissues: leaves, flowers, roots, unripe pulp, ripe pulp, unripe peels, and ripe peels of two different genetic groups: Prata-Anã (PA; group AAB) and Grand Naine (GN; group AAA). After the analysis of the expression of genes associated with the promoters, normalization was performed with the most stable reference genes (TUB and L2) selected using the RefFinder tool. It was determined that five genes were specific or expressed to a greater extent in some tissues than others. The EMB-23 gene was highly expressed in ripe pulp and flowers of GN, EMB-26 in the ripe pulp of GN, EMB-27 in flowers of GN, EMB-28 in roots of PA and ripe pulp and roots of GN, and EMB-31 in roots and flowers of GN and PA, and unripe pulp of GN. The in silico analysis was efficient in the identification of spatial/time-specific genes, thereby decreasing analysis time and cost, making future genetic transformation studies focusing on the application of these tissue-specific promoters possible. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-24T14:38:14Z 2022-10-24T14:38:14Z 2022-06-29 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
LIVRAMENTO, K. G. do et al. Gene expression analysis associated with tissue-specific promoters in Musa spp. Acta Scientiarum-Agronomy, [S.l.], v. 44, n. 1, p. 1-9, 2022. DOI: 10.4025/actasciagron.v44i1.55893. http://repositorio.ufla.br/jspui/handle/1/55326 |
identifier_str_mv |
LIVRAMENTO, K. G. do et al. Gene expression analysis associated with tissue-specific promoters in Musa spp. Acta Scientiarum-Agronomy, [S.l.], v. 44, n. 1, p. 1-9, 2022. DOI: 10.4025/actasciagron.v44i1.55893. |
url |
http://repositorio.ufla.br/jspui/handle/1/55326 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá (EDUEM) |
publisher.none.fl_str_mv |
Editora da Universidade Estadual de Maringá (EDUEM) |
dc.source.none.fl_str_mv |
Acta Scientiarum-Agronomy reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1807835174552993792 |