Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/13476 http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/306 |
Resumo: | The effects of water deficit on the gene CaM6PR expression, encoding mannose -6- phosphate reductase, were evaluated in coffee trees in the formation phase of coffee cultivars IAPAR59 ( Villa Sarchi x Timor hybrid HT832 / 2) and RUBI MG 1192 (Mundo Novo x Catuaí ) of Coffea arabica, respectively regarded as tolerant and sensitive to water stress. The cultivars were planted in December 2007 in the experimental field of Embrapa Cerrados - DF ( CPAC ) and culturedfor two years (2008 and 2009 ) with (I) and without ( NI ) irrigation. For each year two assessments were carried out (P1, not stressed, during the rainy season and P2 , dry season ). For both cultivars, the CaM6PR gene expression measured inleaves through quantitative PCR, showed a strong increase in the dry season for non-irrigated plants when compared with irrigated plants. In addition, the expression of this gene was always greater in IAPAR59 than in RUBI MG 1192. Also, therewas an increased expression of this gene in 2008 when compared to 2009. This difference could be a direct consequence of drought stress levels received by plants, since drought conditions in 2008 were more severe than in 2009. Thus, in this work, we propose the use of the CaM6PR gene as a molecular marker to evaluate the stress level of the coffee plants submitted to water deficit. |
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Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídricoAnalysis of the mannose 6 phosphate reductase gene expression in coffee trees submitted to water deficitCoffea arabicaBiological sciencesAbiotic stressEstresse abióticoExpressão gênicaGenes candidatosqPCRGene expressionGene candidatesThe effects of water deficit on the gene CaM6PR expression, encoding mannose -6- phosphate reductase, were evaluated in coffee trees in the formation phase of coffee cultivars IAPAR59 ( Villa Sarchi x Timor hybrid HT832 / 2) and RUBI MG 1192 (Mundo Novo x Catuaí ) of Coffea arabica, respectively regarded as tolerant and sensitive to water stress. The cultivars were planted in December 2007 in the experimental field of Embrapa Cerrados - DF ( CPAC ) and culturedfor two years (2008 and 2009 ) with (I) and without ( NI ) irrigation. For each year two assessments were carried out (P1, not stressed, during the rainy season and P2 , dry season ). For both cultivars, the CaM6PR gene expression measured inleaves through quantitative PCR, showed a strong increase in the dry season for non-irrigated plants when compared with irrigated plants. In addition, the expression of this gene was always greater in IAPAR59 than in RUBI MG 1192. Also, therewas an increased expression of this gene in 2008 when compared to 2009. This difference could be a direct consequence of drought stress levels received by plants, since drought conditions in 2008 were more severe than in 2009. Thus, in this work, we propose the use of the CaM6PR gene as a molecular marker to evaluate the stress level of the coffee plants submitted to water deficit.Os efeitos do déficit hídrico sobre a expressão do gene CaM6PR, codificando a manose-6-fosfato redutase,foram avaliados em cafeeiros em fase de formação das cultivares IAPAR59 (Villa Sarchi x hibrido de Timor HT832/2) eRUBI MG 1192 (Mundo Novo x Catuaí) de Coffea arabica, consideradas respectivamente como tolerante e sensível ao estresse hídrico. As cultivares foram plantadas em dezembro de 2007 no campo experimental da Embrapa Cerrados – DF(CPAC) e cultivadas durante dois anos (2008 e 2009) com (I) e sem (NI) irrigação. Para cada ano, foram realizadas duas avaliações (P1, não estressado, durante a estação chuvosa e P2, estação seca). Para as duas cultivares, a expressão do gene CaM6PR foi medido em folhas por meio da técnica de PCR quantitativa, apresentou um forte aumento na estação seca para as plantas não irrigadas em comparação com as plantas irrigadas. Além disso, a expressão desse gene sempre foi maior no IAPAR59 que no RUBI MG 1192. Também, observou-se uma maior expressão desse gene no ano de 2008,quando comparada ao ano de 2009. Essa diferença poderia ser uma consequência direta dos níveis de estresse hídrico recebidos pelas plantas, já que as condições da seca em 2008 foram mais severas do que no ano de 2009. Assim,nesse trabalho, propõe-se o uso do gene CaM6PR como marcador molecular, para avaliar o nível de estresse das plantas cafeeiras submetidas ao déficit hídrico.2013-04-192017-08-01T20:05:34Z2017-08-01T20:05:34Z2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfFREIRE, L. P. et al. Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico. Coffee Science, Lavras, v. 8, n. 1, p. 15 - 20, jan./mar. 2013.http://repositorio.ufla.br/jspui/handle/1/13476http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/306Coffee Science; v. 8, n. 1 (2013); 17-23Coffee Science; v. 8, n. 1 (2013); 17-23Coffee Science; v. 8, n. 1 (2013); 17-231984-39091809-6875reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAengporhttp://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/306/pdf_111http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/306/pdfhttp://www.coffeescience.ufla.br/index.php/Coffeescience/article/downloadSuppFile/306/129Freire, Luciana PereiraMarraccini, Pierre RogerRodrigues, Gustavo CostaAndrade, Alan Carvalhoinfo:eu-repo/semantics/openAccess2021-11-22T13:10:08Zoai:localhost:1/13476Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-11-22T13:10:08Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico Analysis of the mannose 6 phosphate reductase gene expression in coffee trees submitted to water deficit |
title |
Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico |
spellingShingle |
Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico Freire, Luciana Pereira Coffea arabica Biological sciences Abiotic stress Estresse abiótico Expressão gênica Genes candidatos qPCR Gene expression Gene candidates |
title_short |
Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico |
title_full |
Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico |
title_fullStr |
Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico |
title_full_unstemmed |
Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico |
title_sort |
Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico |
author |
Freire, Luciana Pereira |
author_facet |
Freire, Luciana Pereira Marraccini, Pierre Roger Rodrigues, Gustavo Costa Andrade, Alan Carvalho |
author_role |
author |
author2 |
Marraccini, Pierre Roger Rodrigues, Gustavo Costa Andrade, Alan Carvalho |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Freire, Luciana Pereira Marraccini, Pierre Roger Rodrigues, Gustavo Costa Andrade, Alan Carvalho |
dc.subject.por.fl_str_mv |
Coffea arabica Biological sciences Abiotic stress Estresse abiótico Expressão gênica Genes candidatos qPCR Gene expression Gene candidates |
topic |
Coffea arabica Biological sciences Abiotic stress Estresse abiótico Expressão gênica Genes candidatos qPCR Gene expression Gene candidates |
description |
The effects of water deficit on the gene CaM6PR expression, encoding mannose -6- phosphate reductase, were evaluated in coffee trees in the formation phase of coffee cultivars IAPAR59 ( Villa Sarchi x Timor hybrid HT832 / 2) and RUBI MG 1192 (Mundo Novo x Catuaí ) of Coffea arabica, respectively regarded as tolerant and sensitive to water stress. The cultivars were planted in December 2007 in the experimental field of Embrapa Cerrados - DF ( CPAC ) and culturedfor two years (2008 and 2009 ) with (I) and without ( NI ) irrigation. For each year two assessments were carried out (P1, not stressed, during the rainy season and P2 , dry season ). For both cultivars, the CaM6PR gene expression measured inleaves through quantitative PCR, showed a strong increase in the dry season for non-irrigated plants when compared with irrigated plants. In addition, the expression of this gene was always greater in IAPAR59 than in RUBI MG 1192. Also, therewas an increased expression of this gene in 2008 when compared to 2009. This difference could be a direct consequence of drought stress levels received by plants, since drought conditions in 2008 were more severe than in 2009. Thus, in this work, we propose the use of the CaM6PR gene as a molecular marker to evaluate the stress level of the coffee plants submitted to water deficit. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-19 2013 2017-08-01T20:05:34Z 2017-08-01T20:05:34Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FREIRE, L. P. et al. Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico. Coffee Science, Lavras, v. 8, n. 1, p. 15 - 20, jan./mar. 2013. http://repositorio.ufla.br/jspui/handle/1/13476 http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/306 |
identifier_str_mv |
FREIRE, L. P. et al. Analise da expressão do gene manose 6 fosfato redutase em cafeeiros submetidos ao déficit hídrico. Coffee Science, Lavras, v. 8, n. 1, p. 15 - 20, jan./mar. 2013. |
url |
http://repositorio.ufla.br/jspui/handle/1/13476 http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/306 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/306/pdf_111 http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/306/pdf http://www.coffeescience.ufla.br/index.php/Coffeescience/article/downloadSuppFile/306/129 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.source.none.fl_str_mv |
Coffee Science; v. 8, n. 1 (2013); 17-23 Coffee Science; v. 8, n. 1 (2013); 17-23 Coffee Science; v. 8, n. 1 (2013); 17-23 1984-3909 1809-6875 reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835073770160128 |