A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni

Detalhes bibliográficos
Autor(a) principal: Mourão, Marina de Moraes
Data de Publicação: 2013
Outros Autores: Bitar, Mainá, Lobo, Francisco Pereira, Peconick, Ana Paula, Grynberg, Priscila, Prosdocimi, Francisco, Waisberg, Michael, Cerqueira, Gustavo Coutinho, Macedo, Andréa Mara, Machado, Carlos Renato, Yoshino, Timothy, Franco, Glória Regina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/12063
Resumo: Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of transspliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974).
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spelling A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoniSpliced leaderTrans-splicingRNA interferenceSchistosoma mansoniSchistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of transspliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974).Instituto Oswaldo Cruz, Ministério da Saúde2016-12-14T12:32:37Z2016-12-14T12:32:37Z2013-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMOURÃO, M. de M. et al. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013.http://repositorio.ufla.br/jspui/handle/1/12063Memórias do Instituto Oswaldo Cruzreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAMourão, Marina de MoraesBitar, MaináLobo, Francisco PereiraPeconick, Ana PaulaGrynberg, PriscilaProsdocimi, FranciscoWaisberg, MichaelCerqueira, Gustavo CoutinhoMacedo, Andréa MaraMachado, Carlos RenatoYoshino, TimothyFranco, Glória Reginainfo:eu-repo/semantics/openAccesseng2016-12-14T12:32:37Zoai:localhost:1/12063Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-12-14T12:32:37Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
title A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
spellingShingle A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
Mourão, Marina de Moraes
Spliced leader
Trans-splicing
RNA interference
Schistosoma mansoni
title_short A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
title_full A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
title_fullStr A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
title_full_unstemmed A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
title_sort A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
author Mourão, Marina de Moraes
author_facet Mourão, Marina de Moraes
Bitar, Mainá
Lobo, Francisco Pereira
Peconick, Ana Paula
Grynberg, Priscila
Prosdocimi, Francisco
Waisberg, Michael
Cerqueira, Gustavo Coutinho
Macedo, Andréa Mara
Machado, Carlos Renato
Yoshino, Timothy
Franco, Glória Regina
author_role author
author2 Bitar, Mainá
Lobo, Francisco Pereira
Peconick, Ana Paula
Grynberg, Priscila
Prosdocimi, Francisco
Waisberg, Michael
Cerqueira, Gustavo Coutinho
Macedo, Andréa Mara
Machado, Carlos Renato
Yoshino, Timothy
Franco, Glória Regina
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mourão, Marina de Moraes
Bitar, Mainá
Lobo, Francisco Pereira
Peconick, Ana Paula
Grynberg, Priscila
Prosdocimi, Francisco
Waisberg, Michael
Cerqueira, Gustavo Coutinho
Macedo, Andréa Mara
Machado, Carlos Renato
Yoshino, Timothy
Franco, Glória Regina
dc.subject.por.fl_str_mv Spliced leader
Trans-splicing
RNA interference
Schistosoma mansoni
topic Spliced leader
Trans-splicing
RNA interference
Schistosoma mansoni
description Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to investigate the function of trans-splicing in Schistosoma mansoni through analysis of genes that may be regulated by this mechanism and via silencing SL-containing transcripts through RNA interference. Here, we report our analysis of SL transcript-enriched cDNA libraries from different S. mansoni life stages. Our results show that the trans-splicing mechanism is apparently not associated with specific genes, subcellular localisations or life stages. In cross-species comparisons, even though the sets of genes that are subject to SL trans-splicing regulation appear to differ between organisms, several commonly shared orthologues were observed. Knockdown of transspliced transcripts in sporocysts resulted in a systemic reduction of the expression levels of all tested trans-spliced transcripts; however, the only phenotypic effect observed was diminished larval size. Further studies involving the findings from this work will provide new insights into the role of trans-splicing in the biology of S. mansoni and other organisms. All Expressed Sequence Tags generated in this study were submitted to dbEST as five different libraries. The accessions for each library and for the individual sequences are as follows: (i) adult worms of mixed sexes (LIBEST_027999: JZ139310 - JZ139779), (ii) female adult worms (LIBEST_028000: JZ139780 - JZ140379), (iii) male adult worms (LIBEST_028001: JZ140380 - JZ141002), (iv) eggs (LIBEST_028002: JZ141003 - JZ141497) and (v) schistosomula (LIBEST_028003: JZ141498 - JZ141974).
publishDate 2013
dc.date.none.fl_str_mv 2013-09
2016-12-14T12:32:37Z
2016-12-14T12:32:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MOURÃO, M. de M. et al. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013.
http://repositorio.ufla.br/jspui/handle/1/12063
identifier_str_mv MOURÃO, M. de M. et al. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 108, n. 6, p. 707-717, Sept. 2013.
url http://repositorio.ufla.br/jspui/handle/1/12063
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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