Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes

Detalhes bibliográficos
Autor(a) principal: Almeida, Julieta Andréa Silva de
Data de Publicação: 2014
Outros Autores: Leal, Rebeca Rocha, Carmazini, Valéria Cristina Barbosa, Salomon, Marcus Vinicius, Guerreiro-Filho, Oliveiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/13841
http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/668
Resumo: The vegetative multiplication of Coffea arabica hybrids can be carried out by direct somatic embryogenesis. The objective of this study was to verify if temperature and cytokinins could influence the capacity for direct somatic embryogeneis in arabica genotypes. For this purpose foliar explants taken from adult plants of three C. arabica genotypes, hybrids 812 and 956 and cultivar Catuaí, were inoculated into a culture medium with ½ MS salts, 20.0 g l-1 sucrose and 30 μM 6-benzyladenine (6-BA) or 10 μM 2-isopenteniladenina (2-iP) and submitted to temperatures of 25 and 30 ºC in the absence of light. The treatments were evaluated with respect to the number of somatic embryos formed per foliar explant, at 270 days after the beginning of the experiment. A completely random experimental design was adopted with a 3 x 2 x 2 (genotype x temperature x plant growth regulator) factorial scheme, with ten replications per treatment and two explants in each. Temperature of 30 oC favored significantly the formation of somatic embryos when compared to 25 oC and this response pattern predominated amongst the three genotypes. On the other hand, 6-BA and 2-iso-pentenladenine caused similar answers for the majority of genotypes. The results of this study showed that temperature has a significant influence on the direct somatic embryogenesis capacity of C. arabica genotypes.
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spelling Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypesEfeito da temperatura e citocinina na capacidade de embriogênese somática direta em genótipos de Coffea arabica L.Híbrido de arábicaCiências biológicasArabica hybridEmbrião somático6-benziladenina2-isopenteniladeninaSomatic embryo6-benzyladenine2-isopenteniladeninaThe vegetative multiplication of Coffea arabica hybrids can be carried out by direct somatic embryogenesis. The objective of this study was to verify if temperature and cytokinins could influence the capacity for direct somatic embryogeneis in arabica genotypes. For this purpose foliar explants taken from adult plants of three C. arabica genotypes, hybrids 812 and 956 and cultivar Catuaí, were inoculated into a culture medium with ½ MS salts, 20.0 g l-1 sucrose and 30 μM 6-benzyladenine (6-BA) or 10 μM 2-isopenteniladenina (2-iP) and submitted to temperatures of 25 and 30 ºC in the absence of light. The treatments were evaluated with respect to the number of somatic embryos formed per foliar explant, at 270 days after the beginning of the experiment. A completely random experimental design was adopted with a 3 x 2 x 2 (genotype x temperature x plant growth regulator) factorial scheme, with ten replications per treatment and two explants in each. Temperature of 30 oC favored significantly the formation of somatic embryos when compared to 25 oC and this response pattern predominated amongst the three genotypes. On the other hand, 6-BA and 2-iso-pentenladenine caused similar answers for the majority of genotypes. The results of this study showed that temperature has a significant influence on the direct somatic embryogenesis capacity of C. arabica genotypes.A multiplicação vegetativa dos híbridos de Coffea arabica pode ser obtida pela embriogênese somática direta. Objetivou-se, neste estudo, verificar se a temperatura e as citocininas 6-benziladenina (6-BA) and 2-isopentenladenina (2-iP) poderiam influenciar na capacidade de embriogênese somática direta de genótipos de arábica. Para tanto, explantes foliares provenientes de plantas adultas dos híbridos 812 e 956 e da cultivar Catuai Vermelho foram inoculados em meio de cultura com metade da concentração dos sais de MS, adição de 20 g l-1 de sacarose, 30 μM de 6-BA ou 10 μM de 2-iP, submetidos às temperaturas de 25 e 30 ºC, em ausência de luz. Foi adotado o delineamento inteiramente casualizado em esquema fatorial 3 x 2 x 2 (genótipo x temperatura x regulador de crescimento de planta), com dez repetições por tratamento e dois explantes em cada repetição. Os tratamentos foram avaliados quanto ao número de embriões somáticos formados por explante foliar, aos 270 dias do início do experimento. A temperatura de 30 ºC aumentou significativamente a formação de embriões somáticos quando comparado com 25 ºC. Por outro lado, as citocininas 6-BA e 2-iP causaram respostas similares para a maioria dos genótipos. Os resultados do presente estudo demonstraram que o fator temperatura tem influência significativa na capacidade de embriogênese somática direta de explantes foliares de genótipos de C. arabica.Almeida, Julieta Andréa Silva deAlmeida, Julieta Andréa Silva deLeal, Rebeca RochaCarmazini, Valéria Cristina BarbosaSalomon, Marcus ViniciusGuerreiro-Filho, OliveiroLeal, Rebeca RochaCarmazini, Valéria Cristina BarbosaSalomon, Marcus ViniciusGuerreiro-Filho, Oliveiro2014-07-162017-08-01T20:08:26Z2017-08-01T20:08:26Z2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfALMEIDA, J. A. S. de et al. Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes. Coffee Science, Lavras, v. 9, n. 3, p. 394-399, jul./set. 2014.http://repositorio.ufla.br/jspui/handle/1/13841http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/668Coffee Science; v. 9, n. 3 (2014); 394-399Coffee Science; v. 9, n. 3 (2014); 394-399Coffee Science; v. 9, n. 3 (2014); 394-3991984-39091809-6875reponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAenghttp://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/668/pdf_105http://www.coffeescience.ufla.br/index.php/Coffeescience/article/downloadSuppFile/668/585http://www.coffeescience.ufla.br/index.php/Coffeescience/article/downloadSuppFile/668/586Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccess2021-03-10T19:14:39Zoai:localhost:1/13841Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2021-03-10T19:14:39Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes
Efeito da temperatura e citocinina na capacidade de embriogênese somática direta em genótipos de Coffea arabica L.
title Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes
spellingShingle Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes
Almeida, Julieta Andréa Silva de
Híbrido de arábica
Ciências biológicas
Arabica hybrid
Embrião somático
6-benziladenina
2-isopenteniladenina
Somatic embryo
6-benzyladenine
2-isopenteniladenina
title_short Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes
title_full Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes
title_fullStr Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes
title_full_unstemmed Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes
title_sort Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes
author Almeida, Julieta Andréa Silva de
author_facet Almeida, Julieta Andréa Silva de
Leal, Rebeca Rocha
Carmazini, Valéria Cristina Barbosa
Salomon, Marcus Vinicius
Guerreiro-Filho, Oliveiro
author_role author
author2 Leal, Rebeca Rocha
Carmazini, Valéria Cristina Barbosa
Salomon, Marcus Vinicius
Guerreiro-Filho, Oliveiro
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Almeida, Julieta Andréa Silva de
dc.contributor.author.fl_str_mv Almeida, Julieta Andréa Silva de
Leal, Rebeca Rocha
Carmazini, Valéria Cristina Barbosa
Salomon, Marcus Vinicius
Guerreiro-Filho, Oliveiro
Leal, Rebeca Rocha
Carmazini, Valéria Cristina Barbosa
Salomon, Marcus Vinicius
Guerreiro-Filho, Oliveiro
dc.subject.por.fl_str_mv Híbrido de arábica
Ciências biológicas
Arabica hybrid
Embrião somático
6-benziladenina
2-isopenteniladenina
Somatic embryo
6-benzyladenine
2-isopenteniladenina
topic Híbrido de arábica
Ciências biológicas
Arabica hybrid
Embrião somático
6-benziladenina
2-isopenteniladenina
Somatic embryo
6-benzyladenine
2-isopenteniladenina
description The vegetative multiplication of Coffea arabica hybrids can be carried out by direct somatic embryogenesis. The objective of this study was to verify if temperature and cytokinins could influence the capacity for direct somatic embryogeneis in arabica genotypes. For this purpose foliar explants taken from adult plants of three C. arabica genotypes, hybrids 812 and 956 and cultivar Catuaí, were inoculated into a culture medium with ½ MS salts, 20.0 g l-1 sucrose and 30 μM 6-benzyladenine (6-BA) or 10 μM 2-isopenteniladenina (2-iP) and submitted to temperatures of 25 and 30 ºC in the absence of light. The treatments were evaluated with respect to the number of somatic embryos formed per foliar explant, at 270 days after the beginning of the experiment. A completely random experimental design was adopted with a 3 x 2 x 2 (genotype x temperature x plant growth regulator) factorial scheme, with ten replications per treatment and two explants in each. Temperature of 30 oC favored significantly the formation of somatic embryos when compared to 25 oC and this response pattern predominated amongst the three genotypes. On the other hand, 6-BA and 2-iso-pentenladenine caused similar answers for the majority of genotypes. The results of this study showed that temperature has a significant influence on the direct somatic embryogenesis capacity of C. arabica genotypes.
publishDate 2014
dc.date.none.fl_str_mv 2014-07-16
2014
2017-08-01T20:08:26Z
2017-08-01T20:08:26Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv ALMEIDA, J. A. S. de et al. Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes. Coffee Science, Lavras, v. 9, n. 3, p. 394-399, jul./set. 2014.
http://repositorio.ufla.br/jspui/handle/1/13841
http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/668
identifier_str_mv ALMEIDA, J. A. S. de et al. Effect of temperature and cytokinin on thr capacity of direct somatic embryogenesis in Coffea arabica L. genotypes. Coffee Science, Lavras, v. 9, n. 3, p. 394-399, jul./set. 2014.
url http://repositorio.ufla.br/jspui/handle/1/13841
http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/668
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.coffeescience.ufla.br/index.php/Coffeescience/article/view/668/pdf_105
http://www.coffeescience.ufla.br/index.php/Coffeescience/article/downloadSuppFile/668/585
http://www.coffeescience.ufla.br/index.php/Coffeescience/article/downloadSuppFile/668/586
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv Coffee Science; v. 9, n. 3 (2014); 394-399
Coffee Science; v. 9, n. 3 (2014); 394-399
Coffee Science; v. 9, n. 3 (2014); 394-399
1984-3909
1809-6875
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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