Optimization of jenipapo in vitro seed germination process
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/30146 |
Resumo: | The in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was no effect of the application of exogenous GA3; The pre-soaking in distilled water for 48 h contributes to obtaining better germination rates; And the reduction in MS medium salts, and laminating the pretreatment in the dark maximizes the germination potential of seeds.Therefore, the optimal conditions for in vitro germination of G. americana L. seeds requires pre-soaking in distilled water for 48 hours and inoculation into culture media consisting of 1/2 MS + 15 g L-1 sucrose, with stratification in the dark for 16 days, followed by the transfer to growth chambers with lighting provided by white fluorescent lamps. |
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Optimization of jenipapo in vitro seed germination processOtimização do processo de germinação in vitro de sementes de jenipapoGenipa americana L.In vitro cultureLigth qualityRubiaceaeStratification in the darkCultivo in vitroQualidade de luzEstratificação no escuroThe in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was no effect of the application of exogenous GA3; The pre-soaking in distilled water for 48 h contributes to obtaining better germination rates; And the reduction in MS medium salts, and laminating the pretreatment in the dark maximizes the germination potential of seeds.Therefore, the optimal conditions for in vitro germination of G. americana L. seeds requires pre-soaking in distilled water for 48 hours and inoculation into culture media consisting of 1/2 MS + 15 g L-1 sucrose, with stratification in the dark for 16 days, followed by the transfer to growth chambers with lighting provided by white fluorescent lamps.A germinação in vitro de sementes é uma alternativa eficaz para obtenção de explantes com rapidez e qualidade sanitária. No entanto, sementes de jenipapeiro apresentam germinação lenta e desuniforme. Para tanto, fatores internos e externos às sementes que interferem diretamente no processo, devem ser identificados, visando à adaptação de melhores técnicas para obtenção de mudas. Neste sentido, objetivou-se otimizar a germinação in vitro de sementes de Genipa americana L. por meio da avaliação de diferentes fatores (qualidade de luz, tratamento com GA3, pré-embebição em água destilada, diferentes composições no meio de cultivo e estratificação no escuro). Verificou-se que a germinação das sementes foi indiferente a luz, no entanto, os melhores resultadados foram obtidos sob condições de escuro contínuo; Não houve efeito da aplicação de GA3 exógeno; A pré-embebição em água destilada durante 48h contribui para obtenção de melhores taxas de germinação; E a redução de sais do meio MS, bem como o pré-tratamento de estratificação no escuro maximizam o potencial germinativo das sementes. Portanto, as condições ótimas para germinação in vitro de sementes de G. americana L. requer a pré-embebição em água destilada por 48 horas e a inoculação em meio de cultivo constituído de 1/2 MS + 15 g L-1 de sacarose, com estratificação no escuro por 16 dias, seguida pela transferência a salas de crescimento com iluminação fornecida por lâmpadas fluorescentes branca.Editora da Universidade Federal de Lavras2018-08-23T16:32:11Z2018-08-23T16:32:11Z2016-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfSOUZA, R. R. de et al. Optimization of jenipapo in vitro seed germination process. Ciência e Agrotecnologia, Lavras, v. 40, n. 6, p. 658-664, Nov./Dec. 2016.http://repositorio.ufla.br/jspui/handle/1/30146Ciência e Agrotecnologiareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessSouza, Rafaela Ribeiro dePaiva, Patrícia Duarte de OliveiraSilva, Raphael Reis daReis, Michele Valquíria dosNery, Fernanda CarlotaPaiva, Renatoeng2018-08-23T16:32:11Zoai:localhost:1/30146Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2018-08-23T16:32:11Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Optimization of jenipapo in vitro seed germination process Otimização do processo de germinação in vitro de sementes de jenipapo |
title |
Optimization of jenipapo in vitro seed germination process |
spellingShingle |
Optimization of jenipapo in vitro seed germination process Souza, Rafaela Ribeiro de Genipa americana L. In vitro culture Ligth quality Rubiaceae Stratification in the dark Cultivo in vitro Qualidade de luz Estratificação no escuro |
title_short |
Optimization of jenipapo in vitro seed germination process |
title_full |
Optimization of jenipapo in vitro seed germination process |
title_fullStr |
Optimization of jenipapo in vitro seed germination process |
title_full_unstemmed |
Optimization of jenipapo in vitro seed germination process |
title_sort |
Optimization of jenipapo in vitro seed germination process |
author |
Souza, Rafaela Ribeiro de |
author_facet |
Souza, Rafaela Ribeiro de Paiva, Patrícia Duarte de Oliveira Silva, Raphael Reis da Reis, Michele Valquíria dos Nery, Fernanda Carlota Paiva, Renato |
author_role |
author |
author2 |
Paiva, Patrícia Duarte de Oliveira Silva, Raphael Reis da Reis, Michele Valquíria dos Nery, Fernanda Carlota Paiva, Renato |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Souza, Rafaela Ribeiro de Paiva, Patrícia Duarte de Oliveira Silva, Raphael Reis da Reis, Michele Valquíria dos Nery, Fernanda Carlota Paiva, Renato |
dc.subject.por.fl_str_mv |
Genipa americana L. In vitro culture Ligth quality Rubiaceae Stratification in the dark Cultivo in vitro Qualidade de luz Estratificação no escuro |
topic |
Genipa americana L. In vitro culture Ligth quality Rubiaceae Stratification in the dark Cultivo in vitro Qualidade de luz Estratificação no escuro |
description |
The in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was no effect of the application of exogenous GA3; The pre-soaking in distilled water for 48 h contributes to obtaining better germination rates; And the reduction in MS medium salts, and laminating the pretreatment in the dark maximizes the germination potential of seeds.Therefore, the optimal conditions for in vitro germination of G. americana L. seeds requires pre-soaking in distilled water for 48 hours and inoculation into culture media consisting of 1/2 MS + 15 g L-1 sucrose, with stratification in the dark for 16 days, followed by the transfer to growth chambers with lighting provided by white fluorescent lamps. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12 2018-08-23T16:32:11Z 2018-08-23T16:32:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SOUZA, R. R. de et al. Optimization of jenipapo in vitro seed germination process. Ciência e Agrotecnologia, Lavras, v. 40, n. 6, p. 658-664, Nov./Dec. 2016. http://repositorio.ufla.br/jspui/handle/1/30146 |
identifier_str_mv |
SOUZA, R. R. de et al. Optimization of jenipapo in vitro seed germination process. Ciência e Agrotecnologia, Lavras, v. 40, n. 6, p. 658-664, Nov./Dec. 2016. |
url |
http://repositorio.ufla.br/jspui/handle/1/30146 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
publisher.none.fl_str_mv |
Editora da Universidade Federal de Lavras |
dc.source.none.fl_str_mv |
Ciência e Agrotecnologia reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
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1784549977069977600 |