Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFLA |
Texto Completo: | http://repositorio.ufla.br/jspui/handle/1/12100 http://www.scielo.br/pdf/ni/v13n1/1679-6225-ni-20140066.pdf |
Resumo: | The effects of reduced doses of Ovaprim™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and postthaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not different from that of cPE-treated males, thus these data were pooled. In B. orbignyanus, motility was higher in fresh (99%) than in equilibrated sperm (81%); post-thaw motility dropped to 42%. In P. lineatus, motility was similar in fresh (99%) and equilibrated sperm (92%); post-thaw motility was 73%. Motility decreased as a function of time post-activation, and this decrease was significant after 60 s in fresh and equilibrated sperm, and as soon as 30 s in post-thaw sperm, in both species. Ovaprim™ at ¼ of the recommended dose can successfully replace cPE. |
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Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extractComputer-assisted sperm analyzerCharaciformesCryopreservationOvaprim™Sperm motilityThe effects of reduced doses of Ovaprim™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and postthaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not different from that of cPE-treated males, thus these data were pooled. In B. orbignyanus, motility was higher in fresh (99%) than in equilibrated sperm (81%); post-thaw motility dropped to 42%. In P. lineatus, motility was similar in fresh (99%) and equilibrated sperm (92%); post-thaw motility was 73%. Motility decreased as a function of time post-activation, and this decrease was significant after 60 s in fresh and equilibrated sperm, and as soon as 30 s in post-thaw sperm, in both species. Ovaprim™ at ¼ of the recommended dose can successfully replace cPE.O efeito de doses reduzidas de Ovaprim® (GnRHa + domperidona) na liberação do sêmen de Brycon orbignyanus e Prochilodus lineatus foi avaliado. Além disso, a qualidade do sêmen foi comparada entre as amostras frescas, equilibradas e descongeladas. Os machos receberam dose única e reduzida de Ovaprim® (0,125 ou 0,25 ml/kg); os machos-controle receberam extrato de hipófise (cPE; 3 mg/kg). O sêmen fresco foi avaliado quanto ao volume, concentração, e osmolalidade e pH do plasma seminal. Em seguida, o sêmen foi diluído num meio de congelamento, equilibrado por 15-20 min e congelado em botijão de vapor de nitrogênio (dry-shipper). A motilidade espermática foi analisada durante 60 s pósativação no sêmen fresco, equilibrado e descongelado. A qualidade do sêmen não diferiu entre os machos tratados com Ovaprim® (ambas as doses) ou cPE, assim foi feito um pool desses dados. Em B. orbignyanus, a motilidade foi maior no sêmen fresco (99%) do que no equilibrado (81%); a motilidade do sêmen descongelado caiu para 42%. Em P. lineatus, a motilidade foi semelhante entre o sêmen fresco (99%) e equilibrado (92%); a motilidade do sêmen descongelado foi 73%. A motilidade caiu em função do tempo pós-ativação, e essa queda foi significante após 60 s no sêmen fresco e equilibrado, e tão precoce quanto 30 s no sêmen descongelado, em ambas as espécies. Ovaprim® a ¼ da dose recomendada pode substituir o cPE com sucesso.Sociedade Brasileira de Ictiologia2016-12-20T17:28:26Z2016-12-20T17:28:26Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVIVEIROS, A. T. de M. et al. Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract. Neotropical Ichthyology, Porto Alegre, v. 13, n. 1, p. 157-164, 2015.http://repositorio.ufla.br/jspui/handle/1/12100http://www.scielo.br/pdf/ni/v13n1/1679-6225-ni-20140066.pdfNeotropical Ichthyologyreponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAViveiros, Ana Tereza de MendonçaGonçalves, Antônio Carlos SilveiraNascimento, Ariane Flávia doNascimento, Ariane Flávia doLeal, Marcelo de Castroinfo:eu-repo/semantics/openAccesseng2016-12-20T17:33:57Zoai:localhost:1/12100Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2016-12-20T17:33:57Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false |
dc.title.none.fl_str_mv |
Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract |
title |
Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract |
spellingShingle |
Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract Viveiros, Ana Tereza de Mendonça Computer-assisted sperm analyzer Characiformes Cryopreservation Ovaprim™ Sperm motility |
title_short |
Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract |
title_full |
Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract |
title_fullStr |
Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract |
title_full_unstemmed |
Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract |
title_sort |
Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract |
author |
Viveiros, Ana Tereza de Mendonça |
author_facet |
Viveiros, Ana Tereza de Mendonça Gonçalves, Antônio Carlos Silveira Nascimento, Ariane Flávia do Leal, Marcelo de Castro |
author_role |
author |
author2 |
Gonçalves, Antônio Carlos Silveira Nascimento, Ariane Flávia do Leal, Marcelo de Castro |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Viveiros, Ana Tereza de Mendonça Gonçalves, Antônio Carlos Silveira Nascimento, Ariane Flávia do Nascimento, Ariane Flávia do Leal, Marcelo de Castro |
dc.subject.por.fl_str_mv |
Computer-assisted sperm analyzer Characiformes Cryopreservation Ovaprim™ Sperm motility |
topic |
Computer-assisted sperm analyzer Characiformes Cryopreservation Ovaprim™ Sperm motility |
description |
The effects of reduced doses of Ovaprim™ (GnRHa + domperidone) on sperm release of Brycon orbignyanus and Prochilodus lineatus were evaluated. Furthermore, sperm quality was compared among fresh, equilibrated and postthaw samples. Males received a single and reduced dose of Ovaprim™ (0.125 or 0.25 ml/kg); control males received pituitary extract (cPE; 3 mg/kg). Fresh sperm was evaluated for volume, concentration, seminal plasma osmolality and seminal plasma pH. Then sperm was diluted in a freezing medium, equilibrated for 15-20 min and frozen in nitrogen vapor vessel (dry-shipper). Sperm motility was analyzed during 60 s post-activation in fresh, equilibrated and post-thaw samples. Sperm quality of males treated with Ovaprim™ (both doses) were not different from that of cPE-treated males, thus these data were pooled. In B. orbignyanus, motility was higher in fresh (99%) than in equilibrated sperm (81%); post-thaw motility dropped to 42%. In P. lineatus, motility was similar in fresh (99%) and equilibrated sperm (92%); post-thaw motility was 73%. Motility decreased as a function of time post-activation, and this decrease was significant after 60 s in fresh and equilibrated sperm, and as soon as 30 s in post-thaw sperm, in both species. Ovaprim™ at ¼ of the recommended dose can successfully replace cPE. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2016-12-20T17:28:26Z 2016-12-20T17:28:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
VIVEIROS, A. T. de M. et al. Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract. Neotropical Ichthyology, Porto Alegre, v. 13, n. 1, p. 157-164, 2015. http://repositorio.ufla.br/jspui/handle/1/12100 http://www.scielo.br/pdf/ni/v13n1/1679-6225-ni-20140066.pdf |
identifier_str_mv |
VIVEIROS, A. T. de M. et al. Fresh, equilibrated and post-thaw sperm quality of piracanjuba brycon orbignyanus and streaked prochilod prochilodus lineatus males treated with either salmon gnrha and domperidone or pituitary extract. Neotropical Ichthyology, Porto Alegre, v. 13, n. 1, p. 157-164, 2015. |
url |
http://repositorio.ufla.br/jspui/handle/1/12100 http://www.scielo.br/pdf/ni/v13n1/1679-6225-ni-20140066.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ictiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ictiologia |
dc.source.none.fl_str_mv |
Neotropical Ichthyology reponame:Repositório Institucional da UFLA instname:Universidade Federal de Lavras (UFLA) instacron:UFLA |
instname_str |
Universidade Federal de Lavras (UFLA) |
instacron_str |
UFLA |
institution |
UFLA |
reponame_str |
Repositório Institucional da UFLA |
collection |
Repositório Institucional da UFLA |
repository.name.fl_str_mv |
Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA) |
repository.mail.fl_str_mv |
nivaldo@ufla.br || repositorio.biblioteca@ufla.br |
_version_ |
1807835197317578752 |