Seleção assistida por marcadores para teor de matéria seca e açúcares redutores em tubérculos de batata

• Autor(a) principal: Bhering, Leonardo Lopes
• Data de Publicação: 2009
• Outros Autores: Pinto, César Augusto Brasil Pereira, Benites, Flávio Rodrigo Gandolfi, Leite, Monik Evelin, Silva, Felipe Lopes da
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UFLA
• Texto Completo: http://repositorio.ufla.br/jspui/handle/1/15175
• topic: Batata - Melhoramento genético; Batata - Processamento; Batata - Seleção precoce; Qualidades de tubérculos; Marcadores genéticos; Potato - Genetic improvement; Potato - Processing; Potato - Early Selection; Qualities of tubers; Genetic markers

Early generation selection for clones with high content of tuber dry matter and low levels of reducing sugars is required for potato (Solanum tuberosum L.) processing. Selection of superior clones at early generations became possible with the deployment of genetic markers, and can precisely identify the superior individuals. Therefore, it is necessary to identify genetic markers closely linked to genes of interest to do assisted selection. The aim of this research was to evaluate the efficiency of marker assisted selection with genetic markers previously identified by ANDREU (2004), which are assumed to be associated with dry matter and reducing sugars content in potato tubers. Clones from 20 families were evaluated during the seedling generation (S), first clonal generation (C1) and second clonal generation (C2). The estimated coefficients of correlation for all traits among generations were significant, even though of low magnitude, confirming that selection at early generation based only on phenotypic traits is inviable. A total of sixteen bands were amplified using these markers. However, by multiple stepwise regression, only seven of these bands showed association with the evaluated traits. Moreover, no markers associated with dry matter and reducing sugars content in the C1 were significantly associated with these traits in the C2, suggesting the existence of QTLs x environment interactions. The marker assisted selection resulted less efficient than the phenotypic selection in all cases studied, and thus is not recommended for early generation selection of clones for the processing industry. These results suggest that the markers used are not closely linked to the genes controlling the traits most important for processing. Therefore, it is important to identify new markers closely linked with such traits of interest that could improve the efficiency of marker assisted selection.