Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos

Detalhes bibliográficos
Autor(a) principal: CARVALHO, Bruno Mileno Magalhães de
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFMA
Texto Completo: https://tedebc.ufma.br/jspui/handle/tede/tede/3771
Resumo: Schistosomiasis is a debilitating disease that can affect various tissues of the human body. The immune response starts with a predominance of Th1 cells and gradually changes to the Th2 profile from oviposition, for the formation and maturation of granulomas. While the lung is one of organs most susceptible organs, it is vulnerable to the parasite in both the acute and chronic phases. Within this context, the objective of this work is to describe the histopathological and histomorphometric alterations in the lung of mice infected with Schistosoma mansoni, as well as to investigate correlations with the presence of eosinophils in the blood, parasite eggs and hepatic granulomas. Initially, male Swiss mice were percutaneously infected with 50 cercariae of Schistosoma mansoni and euthanized after 60 (INF60) and 90 days after infection (INF90). At each time point, there was also euthanasia in a group composed of uninfected animals. Blood was obtained by puncture through the orbital plexus for evaluation of leukocytes. The rectum and liver were removed respectively to prove the infection and histomorphometric comparison. While the lungs were removed for histopathological and histomorphometric analysis. The data showed that within sixty days of infection, there was local and systemic eosinophilia (p<0.0001), associated with a tissue inflammatory process, with pre-granulomatous formation and a predominance of granulocytes in the lung tissue, a period in which there were already several mature granulomas in the liver. Ninety days after the infection, the appearance of pulmonary granulomas and a decrease in blood eosinophilia were observed, which was correlated with the number of eggs in the feces (r= -0.8 and p = 0.01) and the number of granulomas in the liver (r= -0.8 and p = 0.01). The data confirm the importance of the participation of eosinophils in the process, being able to discriminate evolutionary moments and the intensity of the disease until the onset of chronicity, as well as exposing a delay in lung disease compared to liver disease. The data presented in this study confirm the need to observe pulmonary schistosomiasis as an individualized disease in its diagnosis, classification, and management.
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spelling NASCIMENTO, Flávia Raquel Fernandes dohttp://lattes.cnpq.br/9073277157401960NASCIMENTO, Flávia Raquel Fernandes dohttp://lattes.cnpq.br/9073277157401960SOUZA, Valdênia Maria Oliveira dehttp://lattes.cnpq.br/8056939101932406AZEVEDO, Conceição de Maria Pedrozo e Silva dehttp://lattes.cnpq.br/1702212486285798GUERRA, Rosane Nassar Meireleshttp://lattes.cnpq.br/2316192786452127http://lattes.cnpq.br/8671328228112724CARVALHO, Bruno Mileno Magalhães de2022-06-27T13:55:57Z2021-09-10CARVALHO, Bruno Mileno Magalhães de. Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos. 2021. 84 f. Dissertação (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021.https://tedebc.ufma.br/jspui/handle/tede/tede/3771Schistosomiasis is a debilitating disease that can affect various tissues of the human body. The immune response starts with a predominance of Th1 cells and gradually changes to the Th2 profile from oviposition, for the formation and maturation of granulomas. While the lung is one of organs most susceptible organs, it is vulnerable to the parasite in both the acute and chronic phases. Within this context, the objective of this work is to describe the histopathological and histomorphometric alterations in the lung of mice infected with Schistosoma mansoni, as well as to investigate correlations with the presence of eosinophils in the blood, parasite eggs and hepatic granulomas. Initially, male Swiss mice were percutaneously infected with 50 cercariae of Schistosoma mansoni and euthanized after 60 (INF60) and 90 days after infection (INF90). At each time point, there was also euthanasia in a group composed of uninfected animals. Blood was obtained by puncture through the orbital plexus for evaluation of leukocytes. The rectum and liver were removed respectively to prove the infection and histomorphometric comparison. While the lungs were removed for histopathological and histomorphometric analysis. The data showed that within sixty days of infection, there was local and systemic eosinophilia (p<0.0001), associated with a tissue inflammatory process, with pre-granulomatous formation and a predominance of granulocytes in the lung tissue, a period in which there were already several mature granulomas in the liver. Ninety days after the infection, the appearance of pulmonary granulomas and a decrease in blood eosinophilia were observed, which was correlated with the number of eggs in the feces (r= -0.8 and p = 0.01) and the number of granulomas in the liver (r= -0.8 and p = 0.01). The data confirm the importance of the participation of eosinophils in the process, being able to discriminate evolutionary moments and the intensity of the disease until the onset of chronicity, as well as exposing a delay in lung disease compared to liver disease. The data presented in this study confirm the need to observe pulmonary schistosomiasis as an individualized disease in its diagnosis, classification, and management.A esquistossomose é uma doença de caráter debilitante que pode acometer vários tecidos do corpo humano. A resposta imunológica se inicia com predomínio de células Th1 e gradualmente é substituída para o perfil Th2 a partir da oviposição, para a formação e maturação dos granulomas. Enquanto o pulmão é um dos órgãos mais suscetíveis, pois está vulnerável ao parasito tanto na fase aguda quanto na fase crônica. Dentro deste contexto, o objetivo do trabalho é descrever as alterações histopatológicas e histomorfométricas no pulmão de camundongos infectados com Schistosoma mansoni bem como, investigar correlações com à presença de eosinófilos no sangue, ovos do parasito e granulomas hepáticos. Inicialmente, camundongos Swiss machos foram infectados via percutânea com 50 cercárias de Schistosoma mansoni e eutanasiados após 60 (INF60) e 90 dias da infecção (INF90). Em cada tempo, também houve eutanásia em um grupo composto por animais não infectados. O sangue foi obtido por punção pelo plexo orbital para avaliação de leucócitos. O reto e fígado foram retirados respectivamente para comprovação da infecção e comparação histomorfométrica. Enquanto os pulmões foram removidos para análise histopatológica e histomorfométrica. Os dados mostraram que em sessenta dias da infecção, havia uma eosinofilia local e sistêmica (p<0,0001), associado ao processo inflamatório tecidual, com formação pré-granulomatosa e predomínio de granulócitos no tecido pulmonar, período em que já havia vários granulomas maduros no fígado. Aos noventa dias da infecção, observou-se o aparecimento de granulomas pulmonares e uma diminuição da eosinofilia sanguínea, a qual mostrou correlação com a quantidade de ovos nas fezes (r= -0,8 e p = 0,01) e número de granulomas no fígado (r= -0,8 e p = 0,01). Os dados ratificam a importância da participação dos eosinófilos no processo podendo ser capaz de discriminar momentos evolutivos e a intensidade da doença até o início da cronificação, como também expõe um retardo da doença pulmonar em comparação a doença hepática. Os dados apresentados neste trabalho ratificam a necessidade de observar a esquistossomose pulmonar como uma doença individualizada em seu diagnóstico, classificação e conduta.Submitted by Jonathan Sousa de Almeida (jonathan.sousa@ufma.br) on 2022-06-27T13:55:57Z No. of bitstreams: 1 BRUNOMILENOMAGALHÃESDECARVALHO.pdf: 5323679 bytes, checksum: b6be357c8c4ef791384cedc4fe535f19 (MD5)Made available in DSpace on 2022-06-27T13:55:57Z (GMT). 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dc.title.por.fl_str_mv Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos
dc.title.alternative.eng.fl_str_mv Histomorphometry of lung granulomas in the acute and chronic phases during murine Schistosoma mansoni infection and analysis of the correlations with blood eosinophilia, presence of eggs and liver granulomas
title Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos
spellingShingle Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos
CARVALHO, Bruno Mileno Magalhães de
Eosinófilos;
Histomorfometria;
Granuloma Pulmonar;
Esquistossomose.
Pulmonary Granuloma;
Eosinophils;
Histomorphometry;
Schistosomiasis.
Ciências da Saúde
title_short Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos
title_full Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos
title_fullStr Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos
title_full_unstemmed Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos
title_sort Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos
author CARVALHO, Bruno Mileno Magalhães de
author_facet CARVALHO, Bruno Mileno Magalhães de
author_role author
dc.contributor.advisor1.fl_str_mv NASCIMENTO, Flávia Raquel Fernandes do
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/9073277157401960
dc.contributor.referee1.fl_str_mv NASCIMENTO, Flávia Raquel Fernandes do
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/9073277157401960
dc.contributor.referee2.fl_str_mv SOUZA, Valdênia Maria Oliveira de
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/8056939101932406
dc.contributor.referee3.fl_str_mv AZEVEDO, Conceição de Maria Pedrozo e Silva de
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/1702212486285798
dc.contributor.referee4.fl_str_mv GUERRA, Rosane Nassar Meireles
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/2316192786452127
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/8671328228112724
dc.contributor.author.fl_str_mv CARVALHO, Bruno Mileno Magalhães de
contributor_str_mv NASCIMENTO, Flávia Raquel Fernandes do
NASCIMENTO, Flávia Raquel Fernandes do
SOUZA, Valdênia Maria Oliveira de
AZEVEDO, Conceição de Maria Pedrozo e Silva de
GUERRA, Rosane Nassar Meireles
dc.subject.por.fl_str_mv Eosinófilos;
Histomorfometria;
Granuloma Pulmonar;
Esquistossomose.
Pulmonary Granuloma;
topic Eosinófilos;
Histomorfometria;
Granuloma Pulmonar;
Esquistossomose.
Pulmonary Granuloma;
Eosinophils;
Histomorphometry;
Schistosomiasis.
Ciências da Saúde
dc.subject.eng.fl_str_mv Eosinophils;
Histomorphometry;
Schistosomiasis.
dc.subject.cnpq.fl_str_mv Ciências da Saúde
description Schistosomiasis is a debilitating disease that can affect various tissues of the human body. The immune response starts with a predominance of Th1 cells and gradually changes to the Th2 profile from oviposition, for the formation and maturation of granulomas. While the lung is one of organs most susceptible organs, it is vulnerable to the parasite in both the acute and chronic phases. Within this context, the objective of this work is to describe the histopathological and histomorphometric alterations in the lung of mice infected with Schistosoma mansoni, as well as to investigate correlations with the presence of eosinophils in the blood, parasite eggs and hepatic granulomas. Initially, male Swiss mice were percutaneously infected with 50 cercariae of Schistosoma mansoni and euthanized after 60 (INF60) and 90 days after infection (INF90). At each time point, there was also euthanasia in a group composed of uninfected animals. Blood was obtained by puncture through the orbital plexus for evaluation of leukocytes. The rectum and liver were removed respectively to prove the infection and histomorphometric comparison. While the lungs were removed for histopathological and histomorphometric analysis. The data showed that within sixty days of infection, there was local and systemic eosinophilia (p<0.0001), associated with a tissue inflammatory process, with pre-granulomatous formation and a predominance of granulocytes in the lung tissue, a period in which there were already several mature granulomas in the liver. Ninety days after the infection, the appearance of pulmonary granulomas and a decrease in blood eosinophilia were observed, which was correlated with the number of eggs in the feces (r= -0.8 and p = 0.01) and the number of granulomas in the liver (r= -0.8 and p = 0.01). The data confirm the importance of the participation of eosinophils in the process, being able to discriminate evolutionary moments and the intensity of the disease until the onset of chronicity, as well as exposing a delay in lung disease compared to liver disease. The data presented in this study confirm the need to observe pulmonary schistosomiasis as an individualized disease in its diagnosis, classification, and management.
publishDate 2021
dc.date.issued.fl_str_mv 2021-09-10
dc.date.accessioned.fl_str_mv 2022-06-27T13:55:57Z
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dc.identifier.citation.fl_str_mv CARVALHO, Bruno Mileno Magalhães de. Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos. 2021. 84 f. Dissertação (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021.
dc.identifier.uri.fl_str_mv https://tedebc.ufma.br/jspui/handle/tede/tede/3771
identifier_str_mv CARVALHO, Bruno Mileno Magalhães de. Histomorfometria de granulomas pulmonares nas fases aguda e crônica durante a infecção murina por Schistosoma mansoni e análise das correlações com eosinofilia sanguínea, presença de ovos e granulomas hepáticos. 2021. 84 f. Dissertação (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021.
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