Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses

Detalhes bibliográficos
Autor(a) principal: LUZ, Tássio Rômulo Silva Araújo
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFMA
Texto Completo: https://tedebc.ufma.br/jspui/handle/tede/tede/3306
Resumo: In the world context, especially in tropical countries, arboviruses have become an important and constant threat to public health, especially those transmitted by Aedes aegypti L. mosquitoes, and the prevention of these diseases is based mainly on vector control. The use of synthetic products for this purpose has shown problems mainly related to mosquito resistance and environmental toxicity, and studies are needed to enable the development of safer and more effective products. Thus, the objective of the study was to perform the bioprospecting of larvicidal activity against Aedes aegypti of aromatic plant species of the local flora, determining the best time of collection to obtain the essential oils through the evaluation of chemical composition, biological activity, and safety against, Artemia salina Leach and Danio rerio Hamilton Buchanan from them, to obtain larvicidal bioproducts. Chapter I comprises a literature review of essential oils and isolated compounds that have been evaluated for their larvicidal activity, and safety, and it has been shown that several oils have this biological activity and safety for use in the environment, guiding future research. Chapter II presents the results of the seasonal bioprospecting of the chemical composition, larvicidal activity and toxicity of the species: Eugenia uniflora L., Lantana camara L., Plectranthus neochilus Schltr and Ocimum basilicum L.; The chemical analysis performed by chromatography coupled to mass spectrometry showed curzerene, germacrene D, caryophyllene and methylchavicol/linalool as major compounds, respectively. Although the species presented larvicidal activity against Aedes aegypti, the essential oils showed toxicity against the non-target organisms evaluated, and additional safety studies are required. Chapter III deals with the influence of seasonality on the chemical composition, larvicidal and toxicological activity of a rare chemotype of the species Cinamomum verum J. Perls, characterized by the presence of the major compound cinnamyl acetate, and despite the larvicidal activity, the essential oil showed high toxicity against non-target organisms and further safety studies are needed to validate the use of this oil in the environment. Chapter IV deals with the influence of seasonality on the chemical composition, larvicidal and toxicological activity of Mesosphaerum suaveolens (L.) Kuntze species, where GC-MS analysis showed qualitative and quantitative changes, with 1,8-cineol as the major compound in all species the evaluated times. The oils showed larvicidal activity against A. aegypti, with lethal concentrations 90.8-135.2 μg/mL, and their isolated compounds showed no activity up to the highest concentration tested, the oils also showed lower toxicity against A. salina and D. rerio, with concentrations ranging from 94.6-215.7 μg/mL, demonstrating the potential of the species essential oil for production of larvicidal bioproducts. Chapter V describes the possible mechanism of larvicidal action of the essential oil of M. suaveolens through the enzyme inhibition of Acetylcholinesterase (AChE), in addition to detailing structural changes in the larva caused by the essential oil. In the general context the bioprospecting of aromatic species showed that the oils are effective against A. aegypti larvae, that seasonality influences the chemical composition and biological activities, being the collection in the dry season more promising for most essential oils, can generate larvicidal agents to be used in the control of arboviruses.
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spelling COUTINHO, Denise Fernandeshttp://lattes.cnpq.br/7346399893912346AMARAL, Flavia Maria Mendonça dohttp://lattes.cnpq.br/9334507801916334COUTINHO, Denise Fernandeshttp://lattes.cnpq.br/7346399893912346AMARAL, Flavia Maria Mendonça dohttp://lattes.cnpq.br/9334507801916334ROCHA, Cláudia Quintino dahttp://lattes.cnpq.br/5609489233382242DIAS, Clarice Noletohttp://lattes.cnpq.br/8044369355384082VILANOVA, Crisálida Machadohttp://lattes.cnpq.br/6169887955326331http://lattes.cnpq.br/5646604104428602LUZ, Tássio Rômulo Silva Araújo2021-05-17T12:39:57Z2021-02-24LUZ, Tássio Rômulo Silva Araújo. Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses. 2021. 216 f. Tese (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021.https://tedebc.ufma.br/jspui/handle/tede/tede/3306In the world context, especially in tropical countries, arboviruses have become an important and constant threat to public health, especially those transmitted by Aedes aegypti L. mosquitoes, and the prevention of these diseases is based mainly on vector control. The use of synthetic products for this purpose has shown problems mainly related to mosquito resistance and environmental toxicity, and studies are needed to enable the development of safer and more effective products. Thus, the objective of the study was to perform the bioprospecting of larvicidal activity against Aedes aegypti of aromatic plant species of the local flora, determining the best time of collection to obtain the essential oils through the evaluation of chemical composition, biological activity, and safety against, Artemia salina Leach and Danio rerio Hamilton Buchanan from them, to obtain larvicidal bioproducts. Chapter I comprises a literature review of essential oils and isolated compounds that have been evaluated for their larvicidal activity, and safety, and it has been shown that several oils have this biological activity and safety for use in the environment, guiding future research. Chapter II presents the results of the seasonal bioprospecting of the chemical composition, larvicidal activity and toxicity of the species: Eugenia uniflora L., Lantana camara L., Plectranthus neochilus Schltr and Ocimum basilicum L.; The chemical analysis performed by chromatography coupled to mass spectrometry showed curzerene, germacrene D, caryophyllene and methylchavicol/linalool as major compounds, respectively. Although the species presented larvicidal activity against Aedes aegypti, the essential oils showed toxicity against the non-target organisms evaluated, and additional safety studies are required. Chapter III deals with the influence of seasonality on the chemical composition, larvicidal and toxicological activity of a rare chemotype of the species Cinamomum verum J. Perls, characterized by the presence of the major compound cinnamyl acetate, and despite the larvicidal activity, the essential oil showed high toxicity against non-target organisms and further safety studies are needed to validate the use of this oil in the environment. Chapter IV deals with the influence of seasonality on the chemical composition, larvicidal and toxicological activity of Mesosphaerum suaveolens (L.) Kuntze species, where GC-MS analysis showed qualitative and quantitative changes, with 1,8-cineol as the major compound in all species the evaluated times. The oils showed larvicidal activity against A. aegypti, with lethal concentrations 90.8-135.2 μg/mL, and their isolated compounds showed no activity up to the highest concentration tested, the oils also showed lower toxicity against A. salina and D. rerio, with concentrations ranging from 94.6-215.7 μg/mL, demonstrating the potential of the species essential oil for production of larvicidal bioproducts. Chapter V describes the possible mechanism of larvicidal action of the essential oil of M. suaveolens through the enzyme inhibition of Acetylcholinesterase (AChE), in addition to detailing structural changes in the larva caused by the essential oil. In the general context the bioprospecting of aromatic species showed that the oils are effective against A. aegypti larvae, that seasonality influences the chemical composition and biological activities, being the collection in the dry season more promising for most essential oils, can generate larvicidal agents to be used in the control of arboviruses.No contexto mundial, em especial, nos países tropicais, as arboviroses são importantes e constantes ameaças a saúde pública, com destaque as transmitidas por mosquitos da espécie Aedes aegypti L., com prevenção dessas doenças baseada principalmente no controle vetorial. O uso de produtos sintéticos com essa finalidade tem demonstrado problemas relacionados a resistência dos mosquitos e toxicidade ambiental, sendo necessários estudos que possam viabilizar o desenvolvimento de produtos mais seguros e eficazes. Dessa forma o objetivo da pesquisa foi realizar a bioprospecção da atividade larvicida frente Aedes aegypti L. de espécies vegetais aromáticas da flora maranhense, determinando a melhor época de coleta para obtenção dos óleos essenciais através da avaliação da composição química, atividade biológica e segurança frente Artemia salina Leach e Danio rerio Hamilton Buchanan, além de elucidar o mecanismo de ação da atividade larvicida. O capítulo I compreende uma revisão de literatura sobre óleos essenciais e compostos isolados que foram avaliados quanto a sua atividade larvicida e segurança, sendo demonstrado que diversos óleos possuem potencial para serem utilizados sem impacto ao meio ambiente, além de direcionar pesquisas futuras. O capítulo II apresenta o resultado da bioprospecção sazonal fundamentada na investigação da composição química, atividade larvicida e toxicidade das espécies: Eugenia uniflora L., Lantana camara L., Plectranthus neochilus Schltr e Ocimum basilicum L.; com análise química realizada por cromatografia gasosa acoplada a espectrometria de massas evidenciando curzereno, germacreno D, cariofileno e metilchavicol/linalol como compostos majoritários, respectivamente. Apesar das espécies apresentarem atividade larvicida frente Aedes aegypti, os óleos essenciais demonstraram toxicidade frente aos organismos não alvo avaliados (A. salina e D. rerio), sendo necessários estudos complementares de segurança. O capítulo III aborda a influência da sazonalidade na composição química, atividade larvicida e toxicológica de um novo quimiotipo para espécie Cinamomum verum J. Perls, caracterizado pela presença do composto majoritário acetato de cinamila, com comprovada atividade larvicida do óleo essencial, mas elevada toxicidade frente organismo não alvo, sendo necessário mais estudos de segurança para validar o uso desse óleo no meio ambiente. O capítulo IV aborda a influência da sazonalidade na composição química, atividade larvicida e toxicológica da espécie Mesosphaerum suaveolens (L.) Kuntze, onde a análise por CG-EM demonstrou mudanças qualitativas e quantitativas nas amostras obtidas em diferentes períodos de coleta, constatando 1,8-cineol como composto majoritário em todas as épocas avaliadas; evidenciando que os óleos apresentaram atividade larvicida frente A. aegypti, com concentrações letais 90,8-135,2 μg/mL; enquanto seus compostos isolados não apresentaram atividade até a maior concentração testada (200 μg/mL); demonstrando, ainda, que os óleos também apresentaram menor toxicidade frente A. salina e D. rerio, com concentrações que variaram de 94,6-215,7 μg/mL, evidenciando o potencial do óleo essencial da espécie para produção de bioprodutos larvicidas. O capítulo V descreve o possível mecanismo de ação larvicida do óleo essencial de M. suaveolens através da inibição enzimática da Acetilcolinesterase (AChE), além de detalhar alterações estruturais na larva causados pelo óleo essencial. No contexto geral a bioprospecção das espécies aromáticas demonstrou que os óleos essenciais são efetivos frente as larvas de A. aegypti, que a sazonalidade influencia na composição química e nas atividades biológicas, sendo a coleta realizada na estação seca, mais promissora para a maioria dos óleos essenciais, podendo gerar agentes larvicidas a serem usados no controle de arboviroses.Submitted by Sheila MONTEIRO (sheila.monteiro@ufma.br) on 2021-05-17T12:39:57Z No. of bitstreams: 1 Tese PARCIAL - Tássio Rômulo Silva Araújo Luz.pdf: 2014176 bytes, checksum: 38385f155c37ee193aa0caa82398aae8 (MD5)Made available in DSpace on 2021-05-17T12:39:57Z (GMT). No. of bitstreams: 1 Tese PARCIAL - Tássio Rômulo Silva Araújo Luz.pdf: 2014176 bytes, checksum: 38385f155c37ee193aa0caa82398aae8 (MD5) Previous issue date: 2021-02-24Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESFundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão - FAPEMAapplication/pdfporUniversidade Federal do MaranhãoPROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE/CCBSUFMABrasilDEPARTAMENTO DE FARMÁCIA/CCBSLarvicidaArtemia salinaDanio rerioToxicidadeÓleo essencialLarvicidalArtemia salinaDanio rerioToxicityEssential oilCiências da SaúdeBioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arbovirosesBioprospecting of aromatic plant species from the Legal Amazon against Aedes aegypti L. 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dc.title.por.fl_str_mv Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses
dc.title.alternative.eng.fl_str_mv Bioprospecting of aromatic plant species from the Legal Amazon against Aedes aegypti L. (DIPTERA: CULICIDAE): an alternative for the control of arboviruses
title Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses
spellingShingle Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses
LUZ, Tássio Rômulo Silva Araújo
Larvicida
Artemia salina
Danio rerio
Toxicidade
Óleo essencial
Larvicidal
Artemia salina
Danio rerio
Toxicity
Essential oil
Ciências da Saúde
title_short Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses
title_full Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses
title_fullStr Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses
title_full_unstemmed Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses
title_sort Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses
author LUZ, Tássio Rômulo Silva Araújo
author_facet LUZ, Tássio Rômulo Silva Araújo
author_role author
dc.contributor.advisor1.fl_str_mv COUTINHO, Denise Fernandes
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/7346399893912346
dc.contributor.advisor-co1.fl_str_mv AMARAL, Flavia Maria Mendonça do
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/9334507801916334
dc.contributor.referee1.fl_str_mv COUTINHO, Denise Fernandes
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/7346399893912346
dc.contributor.referee2.fl_str_mv AMARAL, Flavia Maria Mendonça do
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/9334507801916334
dc.contributor.referee3.fl_str_mv ROCHA, Cláudia Quintino da
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/5609489233382242
dc.contributor.referee4.fl_str_mv DIAS, Clarice Noleto
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/8044369355384082
dc.contributor.referee5.fl_str_mv VILANOVA, Crisálida Machado
dc.contributor.referee5Lattes.fl_str_mv http://lattes.cnpq.br/6169887955326331
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5646604104428602
dc.contributor.author.fl_str_mv LUZ, Tássio Rômulo Silva Araújo
contributor_str_mv COUTINHO, Denise Fernandes
AMARAL, Flavia Maria Mendonça do
COUTINHO, Denise Fernandes
AMARAL, Flavia Maria Mendonça do
ROCHA, Cláudia Quintino da
DIAS, Clarice Noleto
VILANOVA, Crisálida Machado
dc.subject.por.fl_str_mv Larvicida
Artemia salina
Danio rerio
Toxicidade
Óleo essencial
topic Larvicida
Artemia salina
Danio rerio
Toxicidade
Óleo essencial
Larvicidal
Artemia salina
Danio rerio
Toxicity
Essential oil
Ciências da Saúde
dc.subject.eng.fl_str_mv Larvicidal
Artemia salina
Danio rerio
Toxicity
Essential oil
dc.subject.cnpq.fl_str_mv Ciências da Saúde
description In the world context, especially in tropical countries, arboviruses have become an important and constant threat to public health, especially those transmitted by Aedes aegypti L. mosquitoes, and the prevention of these diseases is based mainly on vector control. The use of synthetic products for this purpose has shown problems mainly related to mosquito resistance and environmental toxicity, and studies are needed to enable the development of safer and more effective products. Thus, the objective of the study was to perform the bioprospecting of larvicidal activity against Aedes aegypti of aromatic plant species of the local flora, determining the best time of collection to obtain the essential oils through the evaluation of chemical composition, biological activity, and safety against, Artemia salina Leach and Danio rerio Hamilton Buchanan from them, to obtain larvicidal bioproducts. Chapter I comprises a literature review of essential oils and isolated compounds that have been evaluated for their larvicidal activity, and safety, and it has been shown that several oils have this biological activity and safety for use in the environment, guiding future research. Chapter II presents the results of the seasonal bioprospecting of the chemical composition, larvicidal activity and toxicity of the species: Eugenia uniflora L., Lantana camara L., Plectranthus neochilus Schltr and Ocimum basilicum L.; The chemical analysis performed by chromatography coupled to mass spectrometry showed curzerene, germacrene D, caryophyllene and methylchavicol/linalool as major compounds, respectively. Although the species presented larvicidal activity against Aedes aegypti, the essential oils showed toxicity against the non-target organisms evaluated, and additional safety studies are required. Chapter III deals with the influence of seasonality on the chemical composition, larvicidal and toxicological activity of a rare chemotype of the species Cinamomum verum J. Perls, characterized by the presence of the major compound cinnamyl acetate, and despite the larvicidal activity, the essential oil showed high toxicity against non-target organisms and further safety studies are needed to validate the use of this oil in the environment. Chapter IV deals with the influence of seasonality on the chemical composition, larvicidal and toxicological activity of Mesosphaerum suaveolens (L.) Kuntze species, where GC-MS analysis showed qualitative and quantitative changes, with 1,8-cineol as the major compound in all species the evaluated times. The oils showed larvicidal activity against A. aegypti, with lethal concentrations 90.8-135.2 μg/mL, and their isolated compounds showed no activity up to the highest concentration tested, the oils also showed lower toxicity against A. salina and D. rerio, with concentrations ranging from 94.6-215.7 μg/mL, demonstrating the potential of the species essential oil for production of larvicidal bioproducts. Chapter V describes the possible mechanism of larvicidal action of the essential oil of M. suaveolens through the enzyme inhibition of Acetylcholinesterase (AChE), in addition to detailing structural changes in the larva caused by the essential oil. In the general context the bioprospecting of aromatic species showed that the oils are effective against A. aegypti larvae, that seasonality influences the chemical composition and biological activities, being the collection in the dry season more promising for most essential oils, can generate larvicidal agents to be used in the control of arboviruses.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-05-17T12:39:57Z
dc.date.issued.fl_str_mv 2021-02-24
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv LUZ, Tássio Rômulo Silva Araújo. Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses. 2021. 216 f. Tese (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021.
dc.identifier.uri.fl_str_mv https://tedebc.ufma.br/jspui/handle/tede/tede/3306
identifier_str_mv LUZ, Tássio Rômulo Silva Araújo. Bioprospecção de espécies vegetais aromáticas da Amazônia Legal frente Aedes aegypti L. (DIPTERA: CULICIDAE): uma alternativa para o controle de arboviroses. 2021. 216 f. Tese (Programa de Pós-Graduação em Ciências da Saúde/CCBS) - Universidade Federal do Maranhão, São Luís, 2021.
url https://tedebc.ufma.br/jspui/handle/tede/tede/3306
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Maranhão
dc.publisher.program.fl_str_mv PROGRAMA DE PÓS-GRADUAÇÃO EM CIÊNCIAS DA SAÚDE/CCBS
dc.publisher.initials.fl_str_mv UFMA
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv DEPARTAMENTO DE FARMÁCIA/CCBS
publisher.none.fl_str_mv Universidade Federal do Maranhão
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFMA
instname:Universidade Federal do Maranhão (UFMA)
instacron:UFMA
instname_str Universidade Federal do Maranhão (UFMA)
instacron_str UFMA
institution UFMA
reponame_str Biblioteca Digital de Teses e Dissertações da UFMA
collection Biblioteca Digital de Teses e Dissertações da UFMA
bitstream.url.fl_str_mv http://tedebc.ufma.br:8080/bitstream/tede/3306/1/license.txt
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repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFMA - Universidade Federal do Maranhão (UFMA)
repository.mail.fl_str_mv repositorio@ufma.br||repositorio@ufma.br
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