In vitro establishment of Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: an endemic species of the São Francisco River Valley

Detalhes bibliográficos
Autor(a) principal: Docha, Anna Luiza Mota
Data de Publicação: 2020
Outros Autores: Oliveira, Leandro Silva, Souza, Nayara dos Santos, Brondani, Gilvano Ebling
Tipo de documento: Artigo
Idioma: por
Título da fonte: Caderno de Ciências Agrárias (Online)
Texto Completo: https://periodicos.ufmg.br/index.php/ccaufmg/article/view/24029
Resumo: Ceiba rubriflora is a native Brazilian species, critically endangered, from region of middle São Francisco River. C. rubriflora's seed production is scarce because of its endemism. Therefore, the micropropagation is presented as a tool for its propagation and seedling production. Thus, the aim of the present study was to evaluate the in vitro establishment of C. rubriflora seeds after disinfestation with different concentrations of active chlorine and testing the in vitro multiplication of apical shoots in different concentrations of 6-benzylaminopurine (BAP). The in vitro establishment of seeds was performed in MS culture medium, which were previously submitted to sodium hypochlorite (NaOCl) disinfection at concentrations of 0.0; 0.5; 1.0; 1.5; and 2.5% active chlorine. Apical shoots of in vitro germinated seedlings were used as explants for multiplication, in MS culture medium, supplemented with BAP in concentrations of 0.0; 1.0 and 2.0 mg L-1. The evaluations were related to seedling survival, fungal and bacterial contamination and number of shoots per explant. The in vitro germination of seeds was satisfactory, with survival rate higher than 98%. The percentage of in vitro contamination was only 2%, free from bacterial cultures. The apical shoots of C. rubriflora were not multiplied in a single subculture in vitro in BAP culture medium. The results demonstrate the viability of in vitro germination of C. rubriflora seeds for the introduction and subsequent micropropagation process, opening perspectives for further studies with the species.
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spelling In vitro establishment of Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: an endemic species of the São Francisco River ValleyEstabelecimento in vitro de Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: uma espécie endêmica do vale do rio São FranciscoPaineira rubiMata secaConservação de espécieMicropropagaçãoRuby paineiraCalcareous Dry ForestSpecies conservationMicropropagationCeiba rubriflora is a native Brazilian species, critically endangered, from region of middle São Francisco River. C. rubriflora's seed production is scarce because of its endemism. Therefore, the micropropagation is presented as a tool for its propagation and seedling production. Thus, the aim of the present study was to evaluate the in vitro establishment of C. rubriflora seeds after disinfestation with different concentrations of active chlorine and testing the in vitro multiplication of apical shoots in different concentrations of 6-benzylaminopurine (BAP). The in vitro establishment of seeds was performed in MS culture medium, which were previously submitted to sodium hypochlorite (NaOCl) disinfection at concentrations of 0.0; 0.5; 1.0; 1.5; and 2.5% active chlorine. Apical shoots of in vitro germinated seedlings were used as explants for multiplication, in MS culture medium, supplemented with BAP in concentrations of 0.0; 1.0 and 2.0 mg L-1. The evaluations were related to seedling survival, fungal and bacterial contamination and number of shoots per explant. The in vitro germination of seeds was satisfactory, with survival rate higher than 98%. The percentage of in vitro contamination was only 2%, free from bacterial cultures. The apical shoots of C. rubriflora were not multiplied in a single subculture in vitro in BAP culture medium. The results demonstrate the viability of in vitro germination of C. rubriflora seeds for the introduction and subsequent micropropagation process, opening perspectives for further studies with the species.Ceiba rubriflora é uma espécie nativa da região do médio São Francisco que se encontra em risco crítico de extinção. Em decorrência do endemismo da espécie, a produção de sementes é escassa e a micropropagação apresenta-se como ferramenta para a propagação da espécie. Dessa forma, o objetivo do presente trabalho foi estabelecer in vitro sementes de C. rubriflora após desinfestação com diferentes concentrações de cloro ativo e testar a multiplicação in vitro de brotações apicais em diferentes concentrações de 6-benzilaminopurina (BAP). O estabelecimento in vitro das sementes foi realizado em meio de cultura MS, sendo estas submetidas previamente à desinfestação com hipoclorito de sódio (NaOCl) nas concentrações de 0,0; 0,5; 1,0; 1,5 e 2,5% de cloro ativo. Em condições in vitro, brotações apicais das plântulas germinadas foram utilizadas como explantes para a multiplicação, em meio de cultura MS, suplementado com BAP nas concentrações de 0,0; 1,0 e 2,0 mg L-1. As avaliações foram referentes à sobrevivência das plântulas, à contaminação fúngica e bacteriana e ao número de brotações por explante. A germinação in vitro das sementes foi superior a 98% com taxa de sobrevivência das plântulas maior que 50%. A porcentagem total de contaminação fúngica foi de apenas 2%, isenta de culturas bacterianas. Não houve multiplicação das brotações apicais de C. rubriflora em um único subcultivo in vitro em meio de cultura com BAP. Os resultados demonstram a viabilidade da germinação in vitro de sementes de C. rubriflora para a introdução e posterior processo de micropropagação, abrindo perspectivas para maiores estudos com a espécie.Universidade Federal de Minas Gerais2020-09-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://periodicos.ufmg.br/index.php/ccaufmg/article/view/2402910.35699/2447-6218.2020.24029Agrarian Sciences Journal; Vol. 12 (2020); 1-5Caderno de Ciências Agrárias; v. 12 (2020); 1-52447-62181984-6738reponame:Caderno de Ciências Agrárias (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGporhttps://periodicos.ufmg.br/index.php/ccaufmg/article/view/24029/19928https://periodicos.ufmg.br/index.php/ccaufmg/article/view/24029/19929Copyright (c) 2020 Caderno de Ciências Agráriashttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessDocha, Anna Luiza Mota Oliveira, Leandro SilvaSouza, Nayara dos Santos Brondani, Gilvano Ebling 2022-07-28T18:28:20Zoai:periodicos.ufmg.br:article/24029Revistahttps://periodicos.ufmg.br/index.php/ccaufmgPUBhttps://periodicos.ufmg.br/index.php/ccaufmg/oaiccaufmg@ica.ufmg.br2447-62181984-6738opendoar:2022-07-28T18:28:20Caderno de Ciências Agrárias (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv In vitro establishment of Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: an endemic species of the São Francisco River Valley
Estabelecimento in vitro de Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: uma espécie endêmica do vale do rio São Francisco
title In vitro establishment of Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: an endemic species of the São Francisco River Valley
spellingShingle In vitro establishment of Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: an endemic species of the São Francisco River Valley
Docha, Anna Luiza Mota
Paineira rubi
Mata seca
Conservação de espécie
Micropropagação
Ruby paineira
Calcareous Dry Forest
Species conservation
Micropropagation
title_short In vitro establishment of Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: an endemic species of the São Francisco River Valley
title_full In vitro establishment of Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: an endemic species of the São Francisco River Valley
title_fullStr In vitro establishment of Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: an endemic species of the São Francisco River Valley
title_full_unstemmed In vitro establishment of Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: an endemic species of the São Francisco River Valley
title_sort In vitro establishment of Ceiba rubriflora Carv.-Sobr. e L. P. Queiroz: an endemic species of the São Francisco River Valley
author Docha, Anna Luiza Mota
author_facet Docha, Anna Luiza Mota
Oliveira, Leandro Silva
Souza, Nayara dos Santos
Brondani, Gilvano Ebling
author_role author
author2 Oliveira, Leandro Silva
Souza, Nayara dos Santos
Brondani, Gilvano Ebling
author2_role author
author
author
dc.contributor.author.fl_str_mv Docha, Anna Luiza Mota
Oliveira, Leandro Silva
Souza, Nayara dos Santos
Brondani, Gilvano Ebling
dc.subject.por.fl_str_mv Paineira rubi
Mata seca
Conservação de espécie
Micropropagação
Ruby paineira
Calcareous Dry Forest
Species conservation
Micropropagation
topic Paineira rubi
Mata seca
Conservação de espécie
Micropropagação
Ruby paineira
Calcareous Dry Forest
Species conservation
Micropropagation
description Ceiba rubriflora is a native Brazilian species, critically endangered, from region of middle São Francisco River. C. rubriflora's seed production is scarce because of its endemism. Therefore, the micropropagation is presented as a tool for its propagation and seedling production. Thus, the aim of the present study was to evaluate the in vitro establishment of C. rubriflora seeds after disinfestation with different concentrations of active chlorine and testing the in vitro multiplication of apical shoots in different concentrations of 6-benzylaminopurine (BAP). The in vitro establishment of seeds was performed in MS culture medium, which were previously submitted to sodium hypochlorite (NaOCl) disinfection at concentrations of 0.0; 0.5; 1.0; 1.5; and 2.5% active chlorine. Apical shoots of in vitro germinated seedlings were used as explants for multiplication, in MS culture medium, supplemented with BAP in concentrations of 0.0; 1.0 and 2.0 mg L-1. The evaluations were related to seedling survival, fungal and bacterial contamination and number of shoots per explant. The in vitro germination of seeds was satisfactory, with survival rate higher than 98%. The percentage of in vitro contamination was only 2%, free from bacterial cultures. The apical shoots of C. rubriflora were not multiplied in a single subculture in vitro in BAP culture medium. The results demonstrate the viability of in vitro germination of C. rubriflora seeds for the introduction and subsequent micropropagation process, opening perspectives for further studies with the species.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-25
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufmg.br/index.php/ccaufmg/article/view/24029
10.35699/2447-6218.2020.24029
url https://periodicos.ufmg.br/index.php/ccaufmg/article/view/24029
identifier_str_mv 10.35699/2447-6218.2020.24029
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufmg.br/index.php/ccaufmg/article/view/24029/19928
https://periodicos.ufmg.br/index.php/ccaufmg/article/view/24029/19929
dc.rights.driver.fl_str_mv Copyright (c) 2020 Caderno de Ciências Agrárias
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Caderno de Ciências Agrárias
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv Agrarian Sciences Journal; Vol. 12 (2020); 1-5
Caderno de Ciências Agrárias; v. 12 (2020); 1-5
2447-6218
1984-6738
reponame:Caderno de Ciências Agrárias (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Caderno de Ciências Agrárias (Online)
collection Caderno de Ciências Agrárias (Online)
repository.name.fl_str_mv Caderno de Ciências Agrárias (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv ccaufmg@ica.ufmg.br
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