Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009 |
Resumo: | The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture. |
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Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro culturedcaprinepreantral follicleα-tocopherolternatinThe effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture.Universidade Federal de Minas Gerais, Escola de Veterinária2009-02-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.61 n.1 2009reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352009000100009info:eu-repo/semantics/openAccessLima-Verde,I.B.Matos,M.H.T.Bruno,J.B.Martins,F.S.Santos,R.R.Báo,S.N.Luque,M.C.A.Vieira,G.A.B.Silveira,E.R.Rodrigues,A.P.R.Figueiredo,J.R.Oliveira,M.A.L.Lima,P.F.eng2009-05-12T00:00:00Zoai:scielo:S0102-09352009000100009Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2009-05-12T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured |
title |
Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured |
spellingShingle |
Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured Lima-Verde,I.B. caprine preantral follicle α-tocopherol ternatin |
title_short |
Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured |
title_full |
Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured |
title_fullStr |
Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured |
title_full_unstemmed |
Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured |
title_sort |
Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured |
author |
Lima-Verde,I.B. |
author_facet |
Lima-Verde,I.B. Matos,M.H.T. Bruno,J.B. Martins,F.S. Santos,R.R. Báo,S.N. Luque,M.C.A. Vieira,G.A.B. Silveira,E.R. Rodrigues,A.P.R. Figueiredo,J.R. Oliveira,M.A.L. Lima,P.F. |
author_role |
author |
author2 |
Matos,M.H.T. Bruno,J.B. Martins,F.S. Santos,R.R. Báo,S.N. Luque,M.C.A. Vieira,G.A.B. Silveira,E.R. Rodrigues,A.P.R. Figueiredo,J.R. Oliveira,M.A.L. Lima,P.F. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima-Verde,I.B. Matos,M.H.T. Bruno,J.B. Martins,F.S. Santos,R.R. Báo,S.N. Luque,M.C.A. Vieira,G.A.B. Silveira,E.R. Rodrigues,A.P.R. Figueiredo,J.R. Oliveira,M.A.L. Lima,P.F. |
dc.subject.por.fl_str_mv |
caprine preantral follicle α-tocopherol ternatin |
topic |
caprine preantral follicle α-tocopherol ternatin |
description |
The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-09352009000100009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.61 n.1 2009 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220881975574528 |