Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured

Detalhes bibliográficos
Autor(a) principal: Lima-Verde,I.B.
Data de Publicação: 2009
Outros Autores: Matos,M.H.T., Bruno,J.B., Martins,F.S., Santos,R.R., Báo,S.N., Luque,M.C.A., Vieira,G.A.B., Silveira,E.R., Rodrigues,A.P.R., Figueiredo,J.R., Oliveira,M.A.L., Lima,P.F.
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009
Resumo: The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture.
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spelling Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro culturedcaprinepreantral follicleα-tocopherolternatinThe effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture.Universidade Federal de Minas Gerais, Escola de Veterinária2009-02-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.61 n.1 2009reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352009000100009info:eu-repo/semantics/openAccessLima-Verde,I.B.Matos,M.H.T.Bruno,J.B.Martins,F.S.Santos,R.R.Báo,S.N.Luque,M.C.A.Vieira,G.A.B.Silveira,E.R.Rodrigues,A.P.R.Figueiredo,J.R.Oliveira,M.A.L.Lima,P.F.eng2009-05-12T00:00:00Zoai:scielo:S0102-09352009000100009Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2009-05-12T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
title Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
spellingShingle Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
Lima-Verde,I.B.
caprine
preantral follicle
α-tocopherol
ternatin
title_short Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
title_full Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
title_fullStr Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
title_full_unstemmed Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
title_sort Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
author Lima-Verde,I.B.
author_facet Lima-Verde,I.B.
Matos,M.H.T.
Bruno,J.B.
Martins,F.S.
Santos,R.R.
Báo,S.N.
Luque,M.C.A.
Vieira,G.A.B.
Silveira,E.R.
Rodrigues,A.P.R.
Figueiredo,J.R.
Oliveira,M.A.L.
Lima,P.F.
author_role author
author2 Matos,M.H.T.
Bruno,J.B.
Martins,F.S.
Santos,R.R.
Báo,S.N.
Luque,M.C.A.
Vieira,G.A.B.
Silveira,E.R.
Rodrigues,A.P.R.
Figueiredo,J.R.
Oliveira,M.A.L.
Lima,P.F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lima-Verde,I.B.
Matos,M.H.T.
Bruno,J.B.
Martins,F.S.
Santos,R.R.
Báo,S.N.
Luque,M.C.A.
Vieira,G.A.B.
Silveira,E.R.
Rodrigues,A.P.R.
Figueiredo,J.R.
Oliveira,M.A.L.
Lima,P.F.
dc.subject.por.fl_str_mv caprine
preantral follicle
α-tocopherol
ternatin
topic caprine
preantral follicle
α-tocopherol
ternatin
description The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture.
publishDate 2009
dc.date.none.fl_str_mv 2009-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352009000100009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.61 n.1 2009
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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