Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000400671 |
Resumo: | ABSTRACT Infection caused by Aeromonas brings great harm to fish farming. Among the factors associated with bacterial pathogenesis, iron uptake can contribute to the survival and virulence of bacteria within hosts. The aim of this study was to check the presence of genes related to iron uptake in Aeromonas hydrophila deriving from aquatic organisms in the São Francisco Valley and associate the presence of these genes with the ability to grow in media containing different concentrations of iron. The DNAs of 41 isolates were extracted and used in PCRs to verify the presence of the Fur, AmoA and pvcAB genes related to iron uptake. The growth of the isolates belonging to different genetic profiles was verified in culture media containing different iron concentrations. Two isolates were positive for the presence of the Fur gene, seven for the AmoA gene and two for the pvcAB gene. The growth test showed that the low availability of iron did not interfere in the growth of the isolates, nor in the isolate that did not contain any of the genes evaluated in this study, suggesting that the iron uptake’s mechanisms of the tested isolates may be related to other genes and proteins. |
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Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrationsbacterial growthiron uptake genesfish farmingABSTRACT Infection caused by Aeromonas brings great harm to fish farming. Among the factors associated with bacterial pathogenesis, iron uptake can contribute to the survival and virulence of bacteria within hosts. The aim of this study was to check the presence of genes related to iron uptake in Aeromonas hydrophila deriving from aquatic organisms in the São Francisco Valley and associate the presence of these genes with the ability to grow in media containing different concentrations of iron. The DNAs of 41 isolates were extracted and used in PCRs to verify the presence of the Fur, AmoA and pvcAB genes related to iron uptake. The growth of the isolates belonging to different genetic profiles was verified in culture media containing different iron concentrations. Two isolates were positive for the presence of the Fur gene, seven for the AmoA gene and two for the pvcAB gene. The growth test showed that the low availability of iron did not interfere in the growth of the isolates, nor in the isolate that did not contain any of the genes evaluated in this study, suggesting that the iron uptake’s mechanisms of the tested isolates may be related to other genes and proteins.Universidade Federal de Minas Gerais, Escola de Veterinária2022-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000400671Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.74 n.4 2022reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-4162-12491info:eu-repo/semantics/openAccessFreitas,M.R.Araujo,B.N.Soares,R.A.N.Gouveia,J.J.S.Costa,M.M.Gouveia,G.V.eng2022-08-16T00:00:00Zoai:scielo:S0102-09352022000400671Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2022-08-16T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
title |
Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
spellingShingle |
Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations Freitas,M.R. bacterial growth iron uptake genes fish farming |
title_short |
Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
title_full |
Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
title_fullStr |
Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
title_full_unstemmed |
Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
title_sort |
Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
author |
Freitas,M.R. |
author_facet |
Freitas,M.R. Araujo,B.N. Soares,R.A.N. Gouveia,J.J.S. Costa,M.M. Gouveia,G.V. |
author_role |
author |
author2 |
Araujo,B.N. Soares,R.A.N. Gouveia,J.J.S. Costa,M.M. Gouveia,G.V. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Freitas,M.R. Araujo,B.N. Soares,R.A.N. Gouveia,J.J.S. Costa,M.M. Gouveia,G.V. |
dc.subject.por.fl_str_mv |
bacterial growth iron uptake genes fish farming |
topic |
bacterial growth iron uptake genes fish farming |
description |
ABSTRACT Infection caused by Aeromonas brings great harm to fish farming. Among the factors associated with bacterial pathogenesis, iron uptake can contribute to the survival and virulence of bacteria within hosts. The aim of this study was to check the presence of genes related to iron uptake in Aeromonas hydrophila deriving from aquatic organisms in the São Francisco Valley and associate the presence of these genes with the ability to grow in media containing different concentrations of iron. The DNAs of 41 isolates were extracted and used in PCRs to verify the presence of the Fur, AmoA and pvcAB genes related to iron uptake. The growth of the isolates belonging to different genetic profiles was verified in culture media containing different iron concentrations. Two isolates were positive for the presence of the Fur gene, seven for the AmoA gene and two for the pvcAB gene. The growth test showed that the low availability of iron did not interfere in the growth of the isolates, nor in the isolate that did not contain any of the genes evaluated in this study, suggesting that the iron uptake’s mechanisms of the tested isolates may be related to other genes and proteins. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000400671 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000400671 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4162-12491 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.74 n.4 2022 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220895962529792 |