Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation
Autor(a) principal: | |
---|---|
Data de Publicação: | 2005 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600008 |
Resumo: | The effect of cryopreservation in IFN-tau, from bovine embryos produced in vitro was evaluated. Two treated groups (G1= fresh bovine embryos, n=59 and G2= freezed embryos, n=84) were used to study the effect of cryopreservation on IFN-tau secretion. After reaching the blastocyst phase, the embryos were kept on individual culture for additional period of 7 days. On days 3 and 7 after the beginning of embryos cultivation, samples of the media culture were taken for IFN-tau secretion titration. Oocysts taken from follicles ranging from 3 to 5mm in diameter were obtained from ovaries of females at slaughterhouse. The embryos were frozen, after being dehydrated with ethylene glycol (1.8m), conditioned on 0.5ml palletes and frozen. Frozen embryos secreted lower IFN-tau than fresh embryos (P<0.05). At day 7 it was registered higher IFN-tau secretion from trophoblast than at day 3 (P<0.05). The increasing of IFN-tau secretion was observed when the blastocyst began to longed and it was directly related to the embryos development. The synthesis of IFN-tau is related to the capability of development of the blastocyst. Cryopreservation is a method that affects the maternal recognition of pregnancy and the post-freezing embryo development. |
id |
UFMG-8_48e54ecce0836b2e8ceffac3e7c750d1 |
---|---|
oai_identifier_str |
oai:scielo:S0102-09352005000600008 |
network_acronym_str |
UFMG-8 |
network_name_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository_id_str |
|
spelling |
Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservationbovineembryoPIVinterferon-tauThe effect of cryopreservation in IFN-tau, from bovine embryos produced in vitro was evaluated. Two treated groups (G1= fresh bovine embryos, n=59 and G2= freezed embryos, n=84) were used to study the effect of cryopreservation on IFN-tau secretion. After reaching the blastocyst phase, the embryos were kept on individual culture for additional period of 7 days. On days 3 and 7 after the beginning of embryos cultivation, samples of the media culture were taken for IFN-tau secretion titration. Oocysts taken from follicles ranging from 3 to 5mm in diameter were obtained from ovaries of females at slaughterhouse. The embryos were frozen, after being dehydrated with ethylene glycol (1.8m), conditioned on 0.5ml palletes and frozen. Frozen embryos secreted lower IFN-tau than fresh embryos (P<0.05). At day 7 it was registered higher IFN-tau secretion from trophoblast than at day 3 (P<0.05). The increasing of IFN-tau secretion was observed when the blastocyst began to longed and it was directly related to the embryos development. The synthesis of IFN-tau is related to the capability of development of the blastocyst. Cryopreservation is a method that affects the maternal recognition of pregnancy and the post-freezing embryo development.Universidade Federal de Minas Gerais, Escola de Veterinária2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600008Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.57 n.6 2005reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352005000600008info:eu-repo/semantics/openAccessAraújo,M.C.C.Vale Filho,V.R.Ferreira,A.M.Sá,W.F.Barreto Filho,J.B.Camargo,L.S.A.Serapião,R.V.Silva,M.V.G.B.eng2006-04-18T00:00:00Zoai:scielo:S0102-09352005000600008Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2006-04-18T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation |
title |
Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation |
spellingShingle |
Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation Araújo,M.C.C. bovine embryo PIV interferon-tau |
title_short |
Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation |
title_full |
Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation |
title_fullStr |
Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation |
title_full_unstemmed |
Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation |
title_sort |
Interferon tau secretion in cattle embryos in vitro fertilized before and after cryopreservation |
author |
Araújo,M.C.C. |
author_facet |
Araújo,M.C.C. Vale Filho,V.R. Ferreira,A.M. Sá,W.F. Barreto Filho,J.B. Camargo,L.S.A. Serapião,R.V. Silva,M.V.G.B. |
author_role |
author |
author2 |
Vale Filho,V.R. Ferreira,A.M. Sá,W.F. Barreto Filho,J.B. Camargo,L.S.A. Serapião,R.V. Silva,M.V.G.B. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Araújo,M.C.C. Vale Filho,V.R. Ferreira,A.M. Sá,W.F. Barreto Filho,J.B. Camargo,L.S.A. Serapião,R.V. Silva,M.V.G.B. |
dc.subject.por.fl_str_mv |
bovine embryo PIV interferon-tau |
topic |
bovine embryo PIV interferon-tau |
description |
The effect of cryopreservation in IFN-tau, from bovine embryos produced in vitro was evaluated. Two treated groups (G1= fresh bovine embryos, n=59 and G2= freezed embryos, n=84) were used to study the effect of cryopreservation on IFN-tau secretion. After reaching the blastocyst phase, the embryos were kept on individual culture for additional period of 7 days. On days 3 and 7 after the beginning of embryos cultivation, samples of the media culture were taken for IFN-tau secretion titration. Oocysts taken from follicles ranging from 3 to 5mm in diameter were obtained from ovaries of females at slaughterhouse. The embryos were frozen, after being dehydrated with ethylene glycol (1.8m), conditioned on 0.5ml palletes and frozen. Frozen embryos secreted lower IFN-tau than fresh embryos (P<0.05). At day 7 it was registered higher IFN-tau secretion from trophoblast than at day 3 (P<0.05). The increasing of IFN-tau secretion was observed when the blastocyst began to longed and it was directly related to the embryos development. The synthesis of IFN-tau is related to the capability of development of the blastocyst. Cryopreservation is a method that affects the maternal recognition of pregnancy and the post-freezing embryo development. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-09352005000600008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.57 n.6 2005 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220878925266944 |