Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro

Detalhes bibliográficos
Autor(a) principal: Sollecito,N.V.
Data de Publicação: 2019
Outros Autores: Pereira,E.C.M., Grázia,J.G.V., Neves,B.P., Couto,B.V.R., Andrade,V.B., Miranda,M.S., Silva,J.K.R., Borges,A.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300723
Resumo: ABSTRACT The aim of this study was to evaluate the supplementation of embryo culture medium with antioxidant obtained from oily extract of Lippia origanoides on in vitro blastocyst development and quality. Oocytes collected from slaughterhouse ovaries were matured and fertilized in vitro following standard laboratory procedures. Zygotes were cultured in SOF medium supplemented according to the following treatments: T1 embryo culture medium without antioxidant supplementation; T2)50μM/mL Cysteamine; T3)2.5μg/mL; T4)5.0μg/mL and T5)10.0μg/mL of antioxidant obtained from oily extract of Lippia origanoides. On the seventh day of culture, the blastocysts were fixed and evaluated for apoptosis rates, number of total cell and inner cell mass cells by means of the TUNEL Test. The use of antioxidants during cultivation did not increase (P> 0.05) the final blastocyst production rate. The treatments T2, T3, T4 and T5 had the lowest (P< 0.05) apoptotic indexes (4.5±1.1%, 8.4±2.5%, 3.4±1.1% and 5.5±0.9%, respectively) when compared to T1 treatment (10.0±1.4%). The number of inner cell mass did not differ (P> 0.05) among embryos from different treatments. The addition of antioxidant obtained from oily extract of Lippia origanoides reduces the apoptosis rate and improves the quality without increasing the total in vitro production of bovine embryos.
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spelling Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitroantioxidantsapoptosisLippia origanoidesin vitro embryo productionABSTRACT The aim of this study was to evaluate the supplementation of embryo culture medium with antioxidant obtained from oily extract of Lippia origanoides on in vitro blastocyst development and quality. Oocytes collected from slaughterhouse ovaries were matured and fertilized in vitro following standard laboratory procedures. Zygotes were cultured in SOF medium supplemented according to the following treatments: T1 embryo culture medium without antioxidant supplementation; T2)50μM/mL Cysteamine; T3)2.5μg/mL; T4)5.0μg/mL and T5)10.0μg/mL of antioxidant obtained from oily extract of Lippia origanoides. On the seventh day of culture, the blastocysts were fixed and evaluated for apoptosis rates, number of total cell and inner cell mass cells by means of the TUNEL Test. The use of antioxidants during cultivation did not increase (P> 0.05) the final blastocyst production rate. The treatments T2, T3, T4 and T5 had the lowest (P< 0.05) apoptotic indexes (4.5±1.1%, 8.4±2.5%, 3.4±1.1% and 5.5±0.9%, respectively) when compared to T1 treatment (10.0±1.4%). The number of inner cell mass did not differ (P> 0.05) among embryos from different treatments. The addition of antioxidant obtained from oily extract of Lippia origanoides reduces the apoptosis rate and improves the quality without increasing the total in vitro production of bovine embryos.Universidade Federal de Minas Gerais, Escola de Veterinária2019-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300723Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.71 n.3 2019reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-4162-10323info:eu-repo/semantics/openAccessSollecito,N.V.Pereira,E.C.M.Grázia,J.G.V.Neves,B.P.Couto,B.V.R.Andrade,V.B.Miranda,M.S.Silva,J.K.R.Borges,A.M.eng2019-07-02T00:00:00Zoai:scielo:S0102-09352019000300723Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2019-07-02T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
title Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
spellingShingle Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
Sollecito,N.V.
antioxidants
apoptosis
Lippia origanoides
in vitro embryo production
title_short Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
title_full Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
title_fullStr Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
title_full_unstemmed Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
title_sort Antioxidant activity of oily extract obtained from Lippia origanoides improves the quality of bovine embryos produced in vitro
author Sollecito,N.V.
author_facet Sollecito,N.V.
Pereira,E.C.M.
Grázia,J.G.V.
Neves,B.P.
Couto,B.V.R.
Andrade,V.B.
Miranda,M.S.
Silva,J.K.R.
Borges,A.M.
author_role author
author2 Pereira,E.C.M.
Grázia,J.G.V.
Neves,B.P.
Couto,B.V.R.
Andrade,V.B.
Miranda,M.S.
Silva,J.K.R.
Borges,A.M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sollecito,N.V.
Pereira,E.C.M.
Grázia,J.G.V.
Neves,B.P.
Couto,B.V.R.
Andrade,V.B.
Miranda,M.S.
Silva,J.K.R.
Borges,A.M.
dc.subject.por.fl_str_mv antioxidants
apoptosis
Lippia origanoides
in vitro embryo production
topic antioxidants
apoptosis
Lippia origanoides
in vitro embryo production
description ABSTRACT The aim of this study was to evaluate the supplementation of embryo culture medium with antioxidant obtained from oily extract of Lippia origanoides on in vitro blastocyst development and quality. Oocytes collected from slaughterhouse ovaries were matured and fertilized in vitro following standard laboratory procedures. Zygotes were cultured in SOF medium supplemented according to the following treatments: T1 embryo culture medium without antioxidant supplementation; T2)50μM/mL Cysteamine; T3)2.5μg/mL; T4)5.0μg/mL and T5)10.0μg/mL of antioxidant obtained from oily extract of Lippia origanoides. On the seventh day of culture, the blastocysts were fixed and evaluated for apoptosis rates, number of total cell and inner cell mass cells by means of the TUNEL Test. The use of antioxidants during cultivation did not increase (P> 0.05) the final blastocyst production rate. The treatments T2, T3, T4 and T5 had the lowest (P< 0.05) apoptotic indexes (4.5±1.1%, 8.4±2.5%, 3.4±1.1% and 5.5±0.9%, respectively) when compared to T1 treatment (10.0±1.4%). The number of inner cell mass did not differ (P> 0.05) among embryos from different treatments. The addition of antioxidant obtained from oily extract of Lippia origanoides reduces the apoptosis rate and improves the quality without increasing the total in vitro production of bovine embryos.
publishDate 2019
dc.date.none.fl_str_mv 2019-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300723
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000300723
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4162-10323
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.71 n.3 2019
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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