Genotypic evaluation of antimicrobial resistance in Staphylococcus spp. isolated from bovine clinical mastitis

Detalhes bibliográficos
Autor(a) principal: Benites,N.R.
Data de Publicação: 2021
Outros Autores: Hora,A.S., Mello,P.L., Laes,M.A., Brandão,P.E., Melville,P.A., Zuniga,E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000200302
Resumo: ABSTRACT Bovine clinical mastitis caused by Staphylococcus spp. is a serious and widespread disease in the world of dairy farming. Antimicrobial therapy is of fundamental importance in the prevention and treatment of infectious mastitis, but the indiscriminate use of antimicrobials acts as a determining factor for the spread of the disease. The present study evaluated the resistance profiles of 57 Staphylococcus spp. isolated from bovine clinical mastitis to beta-lactams and gentamicin, relating characteristics of phenotype (in vitro susceptibility tests) and genotype (detection and expression of genes encoding resistance - mecA, mecALGA251, blaZ, femA, femB, and aacA-aphD - using PCR and RT-PCR, respectively). One or more genes coding for resistance to different antimicrobials were detected in 50 Staphylococcus spp. isolates. The femA and femB genes were the most frequent (75.4% for both). The observed expression of the genes was as follows: blaZ (60%), femA (39.5%), aacA-aphD (50%), femB (32.6%), mecA (8.3%), and mecALGA251 (0%). Considering the relevance of the genus Staphylococcus to bovine mastitis, this study aimed to elucidate aspects regarding the genotypic and phenotypic profiles of these microorganisms so as to contribute to the development of effective strategies for mastitis control.
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spelling Genotypic evaluation of antimicrobial resistance in Staphylococcus spp. isolated from bovine clinical mastitisstaphylococcal mastitisantimicrobial resistance genesgene expressionABSTRACT Bovine clinical mastitis caused by Staphylococcus spp. is a serious and widespread disease in the world of dairy farming. Antimicrobial therapy is of fundamental importance in the prevention and treatment of infectious mastitis, but the indiscriminate use of antimicrobials acts as a determining factor for the spread of the disease. The present study evaluated the resistance profiles of 57 Staphylococcus spp. isolated from bovine clinical mastitis to beta-lactams and gentamicin, relating characteristics of phenotype (in vitro susceptibility tests) and genotype (detection and expression of genes encoding resistance - mecA, mecALGA251, blaZ, femA, femB, and aacA-aphD - using PCR and RT-PCR, respectively). One or more genes coding for resistance to different antimicrobials were detected in 50 Staphylococcus spp. isolates. The femA and femB genes were the most frequent (75.4% for both). The observed expression of the genes was as follows: blaZ (60%), femA (39.5%), aacA-aphD (50%), femB (32.6%), mecA (8.3%), and mecALGA251 (0%). Considering the relevance of the genus Staphylococcus to bovine mastitis, this study aimed to elucidate aspects regarding the genotypic and phenotypic profiles of these microorganisms so as to contribute to the development of effective strategies for mastitis control.Universidade Federal de Minas Gerais, Escola de Veterinária2021-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000200302Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.73 n.2 2021reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-4162-11984info:eu-repo/semantics/openAccessBenites,N.R.Hora,A.S.Mello,P.L.Laes,M.A.Brandão,P.E.Melville,P.A.Zuniga,E.eng2022-07-11T00:00:00Zoai:scielo:S0102-09352021000200302Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2022-07-11T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Genotypic evaluation of antimicrobial resistance in Staphylococcus spp. isolated from bovine clinical mastitis
title Genotypic evaluation of antimicrobial resistance in Staphylococcus spp. isolated from bovine clinical mastitis
spellingShingle Genotypic evaluation of antimicrobial resistance in Staphylococcus spp. isolated from bovine clinical mastitis
Benites,N.R.
staphylococcal mastitis
antimicrobial resistance genes
gene expression
title_short Genotypic evaluation of antimicrobial resistance in Staphylococcus spp. isolated from bovine clinical mastitis
title_full Genotypic evaluation of antimicrobial resistance in Staphylococcus spp. isolated from bovine clinical mastitis
title_fullStr Genotypic evaluation of antimicrobial resistance in Staphylococcus spp. isolated from bovine clinical mastitis
title_full_unstemmed Genotypic evaluation of antimicrobial resistance in Staphylococcus spp. isolated from bovine clinical mastitis
title_sort Genotypic evaluation of antimicrobial resistance in Staphylococcus spp. isolated from bovine clinical mastitis
author Benites,N.R.
author_facet Benites,N.R.
Hora,A.S.
Mello,P.L.
Laes,M.A.
Brandão,P.E.
Melville,P.A.
Zuniga,E.
author_role author
author2 Hora,A.S.
Mello,P.L.
Laes,M.A.
Brandão,P.E.
Melville,P.A.
Zuniga,E.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Benites,N.R.
Hora,A.S.
Mello,P.L.
Laes,M.A.
Brandão,P.E.
Melville,P.A.
Zuniga,E.
dc.subject.por.fl_str_mv staphylococcal mastitis
antimicrobial resistance genes
gene expression
topic staphylococcal mastitis
antimicrobial resistance genes
gene expression
description ABSTRACT Bovine clinical mastitis caused by Staphylococcus spp. is a serious and widespread disease in the world of dairy farming. Antimicrobial therapy is of fundamental importance in the prevention and treatment of infectious mastitis, but the indiscriminate use of antimicrobials acts as a determining factor for the spread of the disease. The present study evaluated the resistance profiles of 57 Staphylococcus spp. isolated from bovine clinical mastitis to beta-lactams and gentamicin, relating characteristics of phenotype (in vitro susceptibility tests) and genotype (detection and expression of genes encoding resistance - mecA, mecALGA251, blaZ, femA, femB, and aacA-aphD - using PCR and RT-PCR, respectively). One or more genes coding for resistance to different antimicrobials were detected in 50 Staphylococcus spp. isolates. The femA and femB genes were the most frequent (75.4% for both). The observed expression of the genes was as follows: blaZ (60%), femA (39.5%), aacA-aphD (50%), femB (32.6%), mecA (8.3%), and mecALGA251 (0%). Considering the relevance of the genus Staphylococcus to bovine mastitis, this study aimed to elucidate aspects regarding the genotypic and phenotypic profiles of these microorganisms so as to contribute to the development of effective strategies for mastitis control.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000200302
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352021000200302
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4162-11984
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.73 n.2 2021
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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