Effect of probiotic supplemented diet on marine shrimp survival after challenge with Vibrio harveyi

• Autor(a) principal: Vieira,F.N.
• Data de Publicação: 2010
• Outros Autores: Buglione,C.C., Mouriño,J.P.L., Jatobá,A., Martins,M.L., Schleder,D.D., Andreatta,E.R., Barraco,M.A., Vinatea,L.A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000300019
• topic: Litopenaeus vannamei; Lactobacillus plantarum; intestinal microbiota; immunostimulation

The effect of a Lactobacillus plantarum-supplemented diet on shrimp growth, digestive tract bacterial microbiota, survival, and some hemato-immunological parameters after an experimental challenge with Vibrio harveyi was studied. No difference (P&gt;0.05) was observed in shrimp survival (80±6%) and final weight (6.63±0.56g) after 60 days feeding trial. Total bacteria count and Vibrio spp. count in the digestive tract were not diferent (P&gt;0.05) until day 40, but they were lower (P<0.05) in the probiotic-supplemented group on day 60. Total lactic bacteria in the shrimp digestive tract was higher after day 20 in the probiotic-supplemented group. Ten hours after V. harveyi challenge, survival of the probiotic-supplemented group (65.7±2.9%) was higher (P&gt;0.05) than the control group (39.9±4.4%). Bacterial counts in hemolymph and hepatopancreas was lower (P<0.05) in the probiotic-supplemented group than in the control group after V. harveyi challenge. Total hemocyte count and serum agglutination activity were higher (P&gt;0.05) in the probiotic-supplemented group after challenge with V. harveyi. Probiotic-supplemented diet modifies shrimp digestive tract bacterial microbiota, increasing resistance to V. harveyi infection.