In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production

Detalhes bibliográficos
Autor(a) principal: Leopoldino,W.M.
Data de Publicação: 2005
Outros Autores: Lana,R.P., Borges,A.C., Mantovani,H.C., Teixeira,R.M.A., Oliveira,J.S., Jaremtchuk,A.R., Eifert,E.C., Martins,R.G.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600012
Resumo: Ruminal fluid from steers fed on pasture was incubated with artificial media at pH 5.5 and 7.0 in two experiments. In the first, the effect of monensin level on resistance of ruminal bacteria to potassium depletion was evaluated; in the second, effects of the ionophores monensin and lasalocid on ammonia and protein production were quantified. In experiment 1, culture media affected potassium level. The monensin concentration needed to cause half maximal potassium depletion was 2.77µM at pH 5.5 but was 0.056µM at pH 7.0, showing that bacteria incubated at pH 5.5 were more tolerant to monensin than those incubated at pH 7.0. Both ionophores as well as increased acidity caused decreased ammonia production. Both ionophores inhibited ammonia production by 56%, independently of pH. In cultures incubated at pH 5.5 compared to pH 7.0, ammonia production was decreased by 50.5%, independently of the ionophores. Therefore, effects of ionophores and acidity were additive, and the maximum inhibition occurred in the presence of an ionophore at low pH (75.2%). Microbial protein production was lowest when lasalocid was present in a low pH culture medium, causing inhibition of microbial growth.
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spelling In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein productionbacterialasalocidmonensinpHrumenRuminal fluid from steers fed on pasture was incubated with artificial media at pH 5.5 and 7.0 in two experiments. In the first, the effect of monensin level on resistance of ruminal bacteria to potassium depletion was evaluated; in the second, effects of the ionophores monensin and lasalocid on ammonia and protein production were quantified. In experiment 1, culture media affected potassium level. The monensin concentration needed to cause half maximal potassium depletion was 2.77µM at pH 5.5 but was 0.056µM at pH 7.0, showing that bacteria incubated at pH 5.5 were more tolerant to monensin than those incubated at pH 7.0. Both ionophores as well as increased acidity caused decreased ammonia production. Both ionophores inhibited ammonia production by 56%, independently of pH. In cultures incubated at pH 5.5 compared to pH 7.0, ammonia production was decreased by 50.5%, independently of the ionophores. Therefore, effects of ionophores and acidity were additive, and the maximum inhibition occurred in the presence of an ionophore at low pH (75.2%). Microbial protein production was lowest when lasalocid was present in a low pH culture medium, causing inhibition of microbial growth.Universidade Federal de Minas Gerais, Escola de Veterinária2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600012Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.57 n.6 2005reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352005000600012info:eu-repo/semantics/openAccessLeopoldino,W.M.Lana,R.P.Borges,A.C.Mantovani,H.C.Teixeira,R.M.A.Oliveira,J.S.Jaremtchuk,A.R.Eifert,E.C.Martins,R.G.R.eng2006-04-18T00:00:00Zoai:scielo:S0102-09352005000600012Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2006-04-18T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production
title In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production
spellingShingle In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production
Leopoldino,W.M.
bacteria
lasalocid
monensin
pH
rumen
title_short In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production
title_full In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production
title_fullStr In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production
title_full_unstemmed In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production
title_sort In vitro effect of pH on resistance of ruminal bacteria to intracellular potassium depletion, and effect of pH and ionophores on ammonia and microbial protein production
author Leopoldino,W.M.
author_facet Leopoldino,W.M.
Lana,R.P.
Borges,A.C.
Mantovani,H.C.
Teixeira,R.M.A.
Oliveira,J.S.
Jaremtchuk,A.R.
Eifert,E.C.
Martins,R.G.R.
author_role author
author2 Lana,R.P.
Borges,A.C.
Mantovani,H.C.
Teixeira,R.M.A.
Oliveira,J.S.
Jaremtchuk,A.R.
Eifert,E.C.
Martins,R.G.R.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Leopoldino,W.M.
Lana,R.P.
Borges,A.C.
Mantovani,H.C.
Teixeira,R.M.A.
Oliveira,J.S.
Jaremtchuk,A.R.
Eifert,E.C.
Martins,R.G.R.
dc.subject.por.fl_str_mv bacteria
lasalocid
monensin
pH
rumen
topic bacteria
lasalocid
monensin
pH
rumen
description Ruminal fluid from steers fed on pasture was incubated with artificial media at pH 5.5 and 7.0 in two experiments. In the first, the effect of monensin level on resistance of ruminal bacteria to potassium depletion was evaluated; in the second, effects of the ionophores monensin and lasalocid on ammonia and protein production were quantified. In experiment 1, culture media affected potassium level. The monensin concentration needed to cause half maximal potassium depletion was 2.77µM at pH 5.5 but was 0.056µM at pH 7.0, showing that bacteria incubated at pH 5.5 were more tolerant to monensin than those incubated at pH 7.0. Both ionophores as well as increased acidity caused decreased ammonia production. Both ionophores inhibited ammonia production by 56%, independently of pH. In cultures incubated at pH 5.5 compared to pH 7.0, ammonia production was decreased by 50.5%, independently of the ionophores. Therefore, effects of ionophores and acidity were additive, and the maximum inhibition occurred in the presence of an ionophore at low pH (75.2%). Microbial protein production was lowest when lasalocid was present in a low pH culture medium, causing inhibition of microbial growth.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352005000600012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.57 n.6 2005
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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