Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300001 |
Resumo: | The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was performed in in vitro produced embryos. The results were validated by the sex ratio of in vitro produced embryos using PCR by Y- specific sequences present in bovine male genomic DNA. After determining genetic sex of in vitro produced embryos, the results showed difference (P<0.05) in deviation of sex ratio when comparing the control group (45.2% females) with the other spermatozoa selection procedures (60.6% females) (P<0.05). The sperm selection methods are capable of selecting X-bearing spermatozoa without compromising the spermatozoa fertility (cleavage and blastocyst rates, 70% and 26%, respectively) and were considered relevant methods to be introduced in bovine in vitro produced embryo programs. |
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Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
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Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo productiondensity gradient centrifugationembryo sex ratioPercollswim-upThe purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was performed in in vitro produced embryos. The results were validated by the sex ratio of in vitro produced embryos using PCR by Y- specific sequences present in bovine male genomic DNA. After determining genetic sex of in vitro produced embryos, the results showed difference (P<0.05) in deviation of sex ratio when comparing the control group (45.2% females) with the other spermatozoa selection procedures (60.6% females) (P<0.05). The sperm selection methods are capable of selecting X-bearing spermatozoa without compromising the spermatozoa fertility (cleavage and blastocyst rates, 70% and 26%, respectively) and were considered relevant methods to be introduced in bovine in vitro produced embryo programs.Universidade Federal de Minas Gerais, Escola de Veterinária2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300001Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.64 n.3 2012reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352012000300001info:eu-repo/semantics/openAccessLucio,A.CResende,M.VDernowseck-Meirelles,J.A.Perini,A.P.Oliveira,L.ZMiguel,M.C.VCarmo,A.STomita,S.YAlves,B.C.AFazano,F.A.T.Lima,V.F.M.H.eng2012-07-02T00:00:00Zoai:scielo:S0102-09352012000300001Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2012-07-02T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production |
title |
Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production |
spellingShingle |
Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production Lucio,A.C density gradient centrifugation embryo sex ratio Percoll swim-up |
title_short |
Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production |
title_full |
Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production |
title_fullStr |
Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production |
title_full_unstemmed |
Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production |
title_sort |
Assessment of swim-up and discontinuous density gradient in sperm sex preselection for bovine embryo production |
author |
Lucio,A.C |
author_facet |
Lucio,A.C Resende,M.V Dernowseck-Meirelles,J.A. Perini,A.P. Oliveira,L.Z Miguel,M.C.V Carmo,A.S Tomita,S.Y Alves,B.C.A Fazano,F.A.T. Lima,V.F.M.H. |
author_role |
author |
author2 |
Resende,M.V Dernowseck-Meirelles,J.A. Perini,A.P. Oliveira,L.Z Miguel,M.C.V Carmo,A.S Tomita,S.Y Alves,B.C.A Fazano,F.A.T. Lima,V.F.M.H. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lucio,A.C Resende,M.V Dernowseck-Meirelles,J.A. Perini,A.P. Oliveira,L.Z Miguel,M.C.V Carmo,A.S Tomita,S.Y Alves,B.C.A Fazano,F.A.T. Lima,V.F.M.H. |
dc.subject.por.fl_str_mv |
density gradient centrifugation embryo sex ratio Percoll swim-up |
topic |
density gradient centrifugation embryo sex ratio Percoll swim-up |
description |
The purpose of this work was to associate the modified swim-up method with centrifugation in density gradient for the separation of X-bearing spermatozoa. Sperm viability and integrity were evaluated through the Trypan Blue/Giemsa staining method. Quality control of centrifuged spermatozoa was performed in in vitro produced embryos. The results were validated by the sex ratio of in vitro produced embryos using PCR by Y- specific sequences present in bovine male genomic DNA. After determining genetic sex of in vitro produced embryos, the results showed difference (P<0.05) in deviation of sex ratio when comparing the control group (45.2% females) with the other spermatozoa selection procedures (60.6% females) (P<0.05). The sperm selection methods are capable of selecting X-bearing spermatozoa without compromising the spermatozoa fertility (cleavage and blastocyst rates, 70% and 26%, respectively) and were considered relevant methods to be introduced in bovine in vitro produced embryo programs. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000300001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-09352012000300001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.64 n.3 2012 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220885394980864 |