Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction

Detalhes bibliográficos
Autor(a) principal: Carvalho,R.
Data de Publicação: 2000
Outros Autores: Passos,L.M.F., Oliveira,A.M., Henry,M., Martins,A.S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400002
Resumo: The genome of one equine embryo and three equine semen specimens collected from a Brazilian farm were tested by polymerase chain reaction (PCR) for the presence of EHV-1 and EHV-4-specific timidine kinase (TK) sequences. The PCR detected specific EHV-1 TK gene sequences in all samples tested. The peripheral blood leukocytes (PBL) of the embryo donor mare also was amplified by EHV-1 TK primers. Infectious virus was not recovered from any specimens. The animals did not show any clinical signal of EHV-1 or EHV-4 infections. EHV-4 was not detected in the studied specimens. The results indicate that PCR was more sensitive than virus isolation in cell culture for detecting EHV-1 in semen of carrier horses.
id UFMG-8_e323b7abe9c988c9c4e416089d660175
oai_identifier_str oai:scielo:S0102-09352000000400002
network_acronym_str UFMG-8
network_name_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository_id_str
spelling Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reactionEquinesemenembryoEHV-1EHV-4PCRThe genome of one equine embryo and three equine semen specimens collected from a Brazilian farm were tested by polymerase chain reaction (PCR) for the presence of EHV-1 and EHV-4-specific timidine kinase (TK) sequences. The PCR detected specific EHV-1 TK gene sequences in all samples tested. The peripheral blood leukocytes (PBL) of the embryo donor mare also was amplified by EHV-1 TK primers. Infectious virus was not recovered from any specimens. The animals did not show any clinical signal of EHV-1 or EHV-4 infections. EHV-4 was not detected in the studied specimens. The results indicate that PCR was more sensitive than virus isolation in cell culture for detecting EHV-1 in semen of carrier horses.Universidade Federal de Minas Gerais, Escola de Veterinária2000-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400002Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.52 n.4 2000reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352000000400002info:eu-repo/semantics/openAccessCarvalho,R.Passos,L.M.F.Oliveira,A.M.Henry,M.Martins,A.S.eng2000-09-21T00:00:00Zoai:scielo:S0102-09352000000400002Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2000-09-21T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
title Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
spellingShingle Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
Carvalho,R.
Equine
semen
embryo
EHV-1
EHV-4
PCR
title_short Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
title_full Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
title_fullStr Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
title_full_unstemmed Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
title_sort Detection of equine herpesvirus 1 DNA in a single embryo and in horse semen by polymerase chain reaction
author Carvalho,R.
author_facet Carvalho,R.
Passos,L.M.F.
Oliveira,A.M.
Henry,M.
Martins,A.S.
author_role author
author2 Passos,L.M.F.
Oliveira,A.M.
Henry,M.
Martins,A.S.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho,R.
Passos,L.M.F.
Oliveira,A.M.
Henry,M.
Martins,A.S.
dc.subject.por.fl_str_mv Equine
semen
embryo
EHV-1
EHV-4
PCR
topic Equine
semen
embryo
EHV-1
EHV-4
PCR
description The genome of one equine embryo and three equine semen specimens collected from a Brazilian farm were tested by polymerase chain reaction (PCR) for the presence of EHV-1 and EHV-4-specific timidine kinase (TK) sequences. The PCR detected specific EHV-1 TK gene sequences in all samples tested. The peripheral blood leukocytes (PBL) of the embryo donor mare also was amplified by EHV-1 TK primers. Infectious virus was not recovered from any specimens. The animals did not show any clinical signal of EHV-1 or EHV-4 infections. EHV-4 was not detected in the studied specimens. The results indicate that PCR was more sensitive than virus isolation in cell culture for detecting EHV-1 in semen of carrier horses.
publishDate 2000
dc.date.none.fl_str_mv 2000-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352000000400002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.52 n.4 2000
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
_version_ 1750220876553388032

Registros relacionados