Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles

Detalhes bibliográficos
Autor(a) principal: Domingues,S.F.S.
Data de Publicação: 2003
Outros Autores: Ferreira,H.S., Muniz,J.A.P.C., Lima,A.K.F., Ohashi,O.M., Figueiredo,J.R., Silva,L.D.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009
Resumo: The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.
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spelling Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian folliclesmonkeypreantral follicleisolationovaryCebus apellaThe aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.Universidade Federal de Minas Gerais, Escola de Veterinária2003-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.55 n.3 2003reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352003000300009info:eu-repo/semantics/openAccessDomingues,S.F.S.Ferreira,H.S.Muniz,J.A.P.C.Lima,A.K.F.Ohashi,O.M.Figueiredo,J.R.Silva,L.D.M.eng2003-10-08T00:00:00Zoai:scielo:S0102-09352003000300009Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2003-10-08T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
title Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
spellingShingle Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
Domingues,S.F.S.
monkey
preantral follicle
isolation
ovary
Cebus apella
title_short Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
title_full Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
title_fullStr Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
title_full_unstemmed Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
title_sort Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
author Domingues,S.F.S.
author_facet Domingues,S.F.S.
Ferreira,H.S.
Muniz,J.A.P.C.
Lima,A.K.F.
Ohashi,O.M.
Figueiredo,J.R.
Silva,L.D.M.
author_role author
author2 Ferreira,H.S.
Muniz,J.A.P.C.
Lima,A.K.F.
Ohashi,O.M.
Figueiredo,J.R.
Silva,L.D.M.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Domingues,S.F.S.
Ferreira,H.S.
Muniz,J.A.P.C.
Lima,A.K.F.
Ohashi,O.M.
Figueiredo,J.R.
Silva,L.D.M.
dc.subject.por.fl_str_mv monkey
preantral follicle
isolation
ovary
Cebus apella
topic monkey
preantral follicle
isolation
ovary
Cebus apella
description The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ovaries (n=6) of three C. apella females, two prepubertal and one adult. Ovaries were divided into four equal parts and fragmented with a tissue chopper, adjusted for serial sections at intervals of 250, 500, 750 and 1,000µm, respectively. Isolated follicles were counted in a Neubauer's chamber and classified as primordial, primary or secondary. The number (mean±SE) of preantral follicles isolated from 1/4 ovary varied from 68,330+17,590 (at the 1,000µm cut interval) to 300,830+111,460 (at the 500µm cut interval. The mean diameter of the isolated preantral follicles varied from 11.6µm to 27.8µm.
publishDate 2003
dc.date.none.fl_str_mv 2003-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09352003000300009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.55 n.3 2003
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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