Manutenção in vitro de células IDE8 em dois tipos de soro bovino

Detalhes bibliográficos
Autor(a) principal: Bastos,C.V.
Data de Publicação: 2007
Outros Autores: Vasconcelos,M.M.C., Ribeiro,M.F.B., Passos,L.M.F.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200046
Resumo: The present study had the objective of defining the culture conditions, optimizing the maintenance and expansion of an IDE-8 cell line in Brazil, with the aim to propose its use as a model for in vitro infection and multiplication of Brazilian strains of rickettsia and other hemoparasites. The supplementation of IDE-8 cells with two distinct fetal bovine sera (a Brazilian and an imported) was evaluated. Culture media were changed weekly and subcultures were carried out every 15 days. The development of cultures and subcultures was evaluated by the percentage of viability and cellular morphology. The results indicate that the imported SFB can be replaced by the Brazilian SFB one, as no significant differences (P<0.05) were seen among culture viabilities.
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spelling Manutenção in vitro de células IDE8 em dois tipos de soro bovinobovinoIDE8cultivo in vitrocélulas de carrapatosoro fetal bovinoThe present study had the objective of defining the culture conditions, optimizing the maintenance and expansion of an IDE-8 cell line in Brazil, with the aim to propose its use as a model for in vitro infection and multiplication of Brazilian strains of rickettsia and other hemoparasites. The supplementation of IDE-8 cells with two distinct fetal bovine sera (a Brazilian and an imported) was evaluated. Culture media were changed weekly and subcultures were carried out every 15 days. The development of cultures and subcultures was evaluated by the percentage of viability and cellular morphology. The results indicate that the imported SFB can be replaced by the Brazilian SFB one, as no significant differences (P<0.05) were seen among culture viabilities.Universidade Federal de Minas Gerais, Escola de Veterinária2007-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200046Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.59 n.2 2007reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09352007000200046info:eu-repo/semantics/openAccessBastos,C.V.Vasconcelos,M.M.C.Ribeiro,M.F.B.Passos,L.M.F.por2007-07-03T00:00:00Zoai:scielo:S0102-09352007000200046Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2007-07-03T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Manutenção in vitro de células IDE8 em dois tipos de soro bovino
title Manutenção in vitro de células IDE8 em dois tipos de soro bovino
spellingShingle Manutenção in vitro de células IDE8 em dois tipos de soro bovino
Bastos,C.V.
bovino
IDE8
cultivo in vitro
células de carrapato
soro fetal bovino
title_short Manutenção in vitro de células IDE8 em dois tipos de soro bovino
title_full Manutenção in vitro de células IDE8 em dois tipos de soro bovino
title_fullStr Manutenção in vitro de células IDE8 em dois tipos de soro bovino
title_full_unstemmed Manutenção in vitro de células IDE8 em dois tipos de soro bovino
title_sort Manutenção in vitro de células IDE8 em dois tipos de soro bovino
author Bastos,C.V.
author_facet Bastos,C.V.
Vasconcelos,M.M.C.
Ribeiro,M.F.B.
Passos,L.M.F.
author_role author
author2 Vasconcelos,M.M.C.
Ribeiro,M.F.B.
Passos,L.M.F.
author2_role author
author
author
dc.contributor.author.fl_str_mv Bastos,C.V.
Vasconcelos,M.M.C.
Ribeiro,M.F.B.
Passos,L.M.F.
dc.subject.por.fl_str_mv bovino
IDE8
cultivo in vitro
células de carrapato
soro fetal bovino
topic bovino
IDE8
cultivo in vitro
células de carrapato
soro fetal bovino
description The present study had the objective of defining the culture conditions, optimizing the maintenance and expansion of an IDE-8 cell line in Brazil, with the aim to propose its use as a model for in vitro infection and multiplication of Brazilian strains of rickettsia and other hemoparasites. The supplementation of IDE-8 cells with two distinct fetal bovine sera (a Brazilian and an imported) was evaluated. Culture media were changed weekly and subcultures were carried out every 15 days. The development of cultures and subcultures was evaluated by the percentage of viability and cellular morphology. The results indicate that the imported SFB can be replaced by the Brazilian SFB one, as no significant differences (P<0.05) were seen among culture viabilities.
publishDate 2007
dc.date.none.fl_str_mv 2007-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200046
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200046
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0102-09352007000200046
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.59 n.2 2007
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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