Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis

Detalhes bibliográficos
Autor(a) principal: Toscan,G.
Data de Publicação: 2020
Outros Autores: Cezar,A.S., Bräunig,P., Pereira,G.R., Vargas,A.C., Sangioni,L.A., Gonçalves,P.B.D., Vogel,F.S.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000501719
Resumo: ABSTRACT Neospora caninum is the main etiologic agent of neosporosis in domestic animals and its pathogenesis comprises two characteristic phases: acute and chronic. Rodents are used as experimental models to mimic acute and chronic bovine neosporosis. In this study, we inoculated a total of 27 female gerbils, with different doses of N. caninum tachyzoites aiming to induce chronic disease. DNA was extracted from different organs of each animal after spontaneous death or euthanasia. Encephalic tissues were submitted to a highly sensitive real time PCR aiming to detect chronically infected animals. All the other samples were submitted to standard PCR. A total of 11 gerbils died due to acute neosporosis, as confirmed by N. caninum DNA detection in organs. 5x103 tachyzoites/mL of N. caninum was the dosage of antigen that can induce chronic infection in gerbils. In the encephalon sections of some animals that showed clinical signs of persistent infection, we found 70% positive for the anterior encephalon section, suggesting this area as preferential for cyst formation. Therefore, we determined the doses of tachyzoites that cause acute or chronic infection and detection of positive tissues, preferably, systemic organs during acute and encephalon in chronic phases.
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spelling Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosisMeriones unguiculatuschronic infectionneosporosisencephalonABSTRACT Neospora caninum is the main etiologic agent of neosporosis in domestic animals and its pathogenesis comprises two characteristic phases: acute and chronic. Rodents are used as experimental models to mimic acute and chronic bovine neosporosis. In this study, we inoculated a total of 27 female gerbils, with different doses of N. caninum tachyzoites aiming to induce chronic disease. DNA was extracted from different organs of each animal after spontaneous death or euthanasia. Encephalic tissues were submitted to a highly sensitive real time PCR aiming to detect chronically infected animals. All the other samples were submitted to standard PCR. A total of 11 gerbils died due to acute neosporosis, as confirmed by N. caninum DNA detection in organs. 5x103 tachyzoites/mL of N. caninum was the dosage of antigen that can induce chronic infection in gerbils. In the encephalon sections of some animals that showed clinical signs of persistent infection, we found 70% positive for the anterior encephalon section, suggesting this area as preferential for cyst formation. Therefore, we determined the doses of tachyzoites that cause acute or chronic infection and detection of positive tissues, preferably, systemic organs during acute and encephalon in chronic phases.Universidade Federal de Minas Gerais, Escola de Veterinária2020-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000501719Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.72 n.5 2020reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-4162-11603info:eu-repo/semantics/openAccessToscan,G.Cezar,A.S.Bräunig,P.Pereira,G.R.Vargas,A.C.Sangioni,L.A.Gonçalves,P.B.D.Vogel,F.S.F.eng2020-11-05T00:00:00Zoai:scielo:S0102-09352020000501719Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2020-11-05T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis
title Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis
spellingShingle Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis
Toscan,G.
Meriones unguiculatus
chronic infection
neosporosis
encephalon
title_short Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis
title_full Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis
title_fullStr Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis
title_full_unstemmed Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis
title_sort Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis
author Toscan,G.
author_facet Toscan,G.
Cezar,A.S.
Bräunig,P.
Pereira,G.R.
Vargas,A.C.
Sangioni,L.A.
Gonçalves,P.B.D.
Vogel,F.S.F.
author_role author
author2 Cezar,A.S.
Bräunig,P.
Pereira,G.R.
Vargas,A.C.
Sangioni,L.A.
Gonçalves,P.B.D.
Vogel,F.S.F.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Toscan,G.
Cezar,A.S.
Bräunig,P.
Pereira,G.R.
Vargas,A.C.
Sangioni,L.A.
Gonçalves,P.B.D.
Vogel,F.S.F.
dc.subject.por.fl_str_mv Meriones unguiculatus
chronic infection
neosporosis
encephalon
topic Meriones unguiculatus
chronic infection
neosporosis
encephalon
description ABSTRACT Neospora caninum is the main etiologic agent of neosporosis in domestic animals and its pathogenesis comprises two characteristic phases: acute and chronic. Rodents are used as experimental models to mimic acute and chronic bovine neosporosis. In this study, we inoculated a total of 27 female gerbils, with different doses of N. caninum tachyzoites aiming to induce chronic disease. DNA was extracted from different organs of each animal after spontaneous death or euthanasia. Encephalic tissues were submitted to a highly sensitive real time PCR aiming to detect chronically infected animals. All the other samples were submitted to standard PCR. A total of 11 gerbils died due to acute neosporosis, as confirmed by N. caninum DNA detection in organs. 5x103 tachyzoites/mL of N. caninum was the dosage of antigen that can induce chronic infection in gerbils. In the encephalon sections of some animals that showed clinical signs of persistent infection, we found 70% positive for the anterior encephalon section, suggesting this area as preferential for cyst formation. Therefore, we determined the doses of tachyzoites that cause acute or chronic infection and detection of positive tissues, preferably, systemic organs during acute and encephalon in chronic phases.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000501719
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4162-11603
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.72 n.5 2020
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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