Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000501719 |
Resumo: | ABSTRACT Neospora caninum is the main etiologic agent of neosporosis in domestic animals and its pathogenesis comprises two characteristic phases: acute and chronic. Rodents are used as experimental models to mimic acute and chronic bovine neosporosis. In this study, we inoculated a total of 27 female gerbils, with different doses of N. caninum tachyzoites aiming to induce chronic disease. DNA was extracted from different organs of each animal after spontaneous death or euthanasia. Encephalic tissues were submitted to a highly sensitive real time PCR aiming to detect chronically infected animals. All the other samples were submitted to standard PCR. A total of 11 gerbils died due to acute neosporosis, as confirmed by N. caninum DNA detection in organs. 5x103 tachyzoites/mL of N. caninum was the dosage of antigen that can induce chronic infection in gerbils. In the encephalon sections of some animals that showed clinical signs of persistent infection, we found 70% positive for the anterior encephalon section, suggesting this area as preferential for cyst formation. Therefore, we determined the doses of tachyzoites that cause acute or chronic infection and detection of positive tissues, preferably, systemic organs during acute and encephalon in chronic phases. |
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Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosisMeriones unguiculatuschronic infectionneosporosisencephalonABSTRACT Neospora caninum is the main etiologic agent of neosporosis in domestic animals and its pathogenesis comprises two characteristic phases: acute and chronic. Rodents are used as experimental models to mimic acute and chronic bovine neosporosis. In this study, we inoculated a total of 27 female gerbils, with different doses of N. caninum tachyzoites aiming to induce chronic disease. DNA was extracted from different organs of each animal after spontaneous death or euthanasia. Encephalic tissues were submitted to a highly sensitive real time PCR aiming to detect chronically infected animals. All the other samples were submitted to standard PCR. A total of 11 gerbils died due to acute neosporosis, as confirmed by N. caninum DNA detection in organs. 5x103 tachyzoites/mL of N. caninum was the dosage of antigen that can induce chronic infection in gerbils. In the encephalon sections of some animals that showed clinical signs of persistent infection, we found 70% positive for the anterior encephalon section, suggesting this area as preferential for cyst formation. Therefore, we determined the doses of tachyzoites that cause acute or chronic infection and detection of positive tissues, preferably, systemic organs during acute and encephalon in chronic phases.Universidade Federal de Minas Gerais, Escola de Veterinária2020-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000501719Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.72 n.5 2020reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/1678-4162-11603info:eu-repo/semantics/openAccessToscan,G.Cezar,A.S.Bräunig,P.Pereira,G.R.Vargas,A.C.Sangioni,L.A.Gonçalves,P.B.D.Vogel,F.S.F.eng2020-11-05T00:00:00Zoai:scielo:S0102-09352020000501719Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2020-11-05T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.none.fl_str_mv |
Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis |
title |
Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis |
spellingShingle |
Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis Toscan,G. Meriones unguiculatus chronic infection neosporosis encephalon |
title_short |
Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis |
title_full |
Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis |
title_fullStr |
Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis |
title_full_unstemmed |
Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis |
title_sort |
Neospora caninum DNA distribution in tissues of gerbils as experimental models of chronic neosporosis |
author |
Toscan,G. |
author_facet |
Toscan,G. Cezar,A.S. Bräunig,P. Pereira,G.R. Vargas,A.C. Sangioni,L.A. Gonçalves,P.B.D. Vogel,F.S.F. |
author_role |
author |
author2 |
Cezar,A.S. Bräunig,P. Pereira,G.R. Vargas,A.C. Sangioni,L.A. Gonçalves,P.B.D. Vogel,F.S.F. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Toscan,G. Cezar,A.S. Bräunig,P. Pereira,G.R. Vargas,A.C. Sangioni,L.A. Gonçalves,P.B.D. Vogel,F.S.F. |
dc.subject.por.fl_str_mv |
Meriones unguiculatus chronic infection neosporosis encephalon |
topic |
Meriones unguiculatus chronic infection neosporosis encephalon |
description |
ABSTRACT Neospora caninum is the main etiologic agent of neosporosis in domestic animals and its pathogenesis comprises two characteristic phases: acute and chronic. Rodents are used as experimental models to mimic acute and chronic bovine neosporosis. In this study, we inoculated a total of 27 female gerbils, with different doses of N. caninum tachyzoites aiming to induce chronic disease. DNA was extracted from different organs of each animal after spontaneous death or euthanasia. Encephalic tissues were submitted to a highly sensitive real time PCR aiming to detect chronically infected animals. All the other samples were submitted to standard PCR. A total of 11 gerbils died due to acute neosporosis, as confirmed by N. caninum DNA detection in organs. 5x103 tachyzoites/mL of N. caninum was the dosage of antigen that can induce chronic infection in gerbils. In the encephalon sections of some animals that showed clinical signs of persistent infection, we found 70% positive for the anterior encephalon section, suggesting this area as preferential for cyst formation. Therefore, we determined the doses of tachyzoites that cause acute or chronic infection and detection of positive tissues, preferably, systemic organs during acute and encephalon in chronic phases. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000501719 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000501719 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-4162-11603 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais, Escola de Veterinária |
dc.source.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.72 n.5 2020 reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online) instname:Universidade Federal de Minas Gerais (UFMG) instacron:UFMG |
instname_str |
Universidade Federal de Minas Gerais (UFMG) |
instacron_str |
UFMG |
institution |
UFMG |
reponame_str |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
collection |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) |
repository.name.fl_str_mv |
Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG) |
repository.mail.fl_str_mv |
journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br |
_version_ |
1750220894656004096 |