Baccharis trimera aqueous extract modulates inflammation and nociception in mice
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFMG |
Texto Completo: | https://doi.org/10.1186/s40816-021-00309-w http://hdl.handle.net/1843/56259 https://orcid.org/0000-0003-2665-5200 https://orcid.org/0000-0003-4876-8302 https://orcid.org/0000-0002-3496-6749 https://orcid.org/0000-0003-0620-2984 https://orcid.org/0000-0002-6832-2765 |
Resumo: | Background: The aerial parts of Baccharis trimera (Less.) are frequently used as a tea to treat several diseases. Therefore, the aim of this study was to identify the constituents of an aqueous extract of B. trimera, focusing on their antioxidant, anti-inflammation, and antinociception activities and properties. For that, the researchers performed in vivo assays using the formalin test and Freund’s Complete Adjuvant (FCA) to measure the acute and chronic inflammatory pain in mice. Moreover, the myeloperoxidase enzyme (MPO) was analyzed in the subcutaneous tissue after the FCA injection, together with the counting of lymphocytes in the peripheral blood of the mice. Results: The qualitative phytochemical analysis indicated the presence of flavonoids and saponins in the B. trimera aqueous extract. The high-performance liquid chromatography (HPLC) analyses showed the presence of phenolic compounds, such as chlorogenic acid, ellagic acid, rosmarinic acid, as well as flavonoids, such as rutin, quercetin, and luteolin. The DPPH assay was used in order to measure the antioxidant activity of the aqueous extract of B. trimera and this showed an IC50 of 118.18 ± 1.02 μg/mg. The data from the formalin test demonstrated that a single dose of the aqueous extract of B. trimera was not able to decrease the nociceptive behavior during the neurogenic phase, at any of the tested doses (20, 40, or 80 mg/kg p.o.). However, during the inflammatory phase of this test, the aqueous extract of B. trimera at 80 mg/kg (p.o.) significantly decreased the nociceptive behavior, showing more effectiveness when compared to the other tested doses (p < 0.05). Importantly, in the chronic inflammatory model on the 5th day of treatment, the aqueous extract of B. trimera (80 mg/kg p.o.) significantly reduced mechanical allodynia (p < 0.01), heat thermal hyperalgesia (p < 0.001), and paw edema (p < 0.05). There were no changes in the MPO activity, but the data exhibited an equivalent decrease in the number of lymphocytes in the blood of the mice that were treated with B. trimera (80 mg.kg− 1 p.o.) and diclofenac sodium. Conclusion: Taken together, the present data reinforces the potential of the B. trimera aqueous extract as an antiinflammatory and analgesic compound. |
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2023-07-14T19:38:10Z2023-07-14T19:38:10Z2021-10-267https://doi.org/10.1186/s40816-021-00309-w2199-1197http://hdl.handle.net/1843/56259https://orcid.org/0000-0003-2665-5200https://orcid.org/0000-0003-4876-8302https://orcid.org/0000-0002-3496-6749https://orcid.org/0000-0003-0620-2984https://orcid.org/0000-0002-6832-2765Background: The aerial parts of Baccharis trimera (Less.) are frequently used as a tea to treat several diseases. Therefore, the aim of this study was to identify the constituents of an aqueous extract of B. trimera, focusing on their antioxidant, anti-inflammation, and antinociception activities and properties. For that, the researchers performed in vivo assays using the formalin test and Freund’s Complete Adjuvant (FCA) to measure the acute and chronic inflammatory pain in mice. Moreover, the myeloperoxidase enzyme (MPO) was analyzed in the subcutaneous tissue after the FCA injection, together with the counting of lymphocytes in the peripheral blood of the mice. Results: The qualitative phytochemical analysis indicated the presence of flavonoids and saponins in the B. trimera aqueous extract. The high-performance liquid chromatography (HPLC) analyses showed the presence of phenolic compounds, such as chlorogenic acid, ellagic acid, rosmarinic acid, as well as flavonoids, such as rutin, quercetin, and luteolin. The DPPH assay was used in order to measure the antioxidant activity of the aqueous extract of B. trimera and this showed an IC50 of 118.18 ± 1.02 μg/mg. The data from the formalin test demonstrated that a single dose of the aqueous extract of B. trimera was not able to decrease the nociceptive behavior during the neurogenic phase, at any of the tested doses (20, 40, or 80 mg/kg p.o.). However, during the inflammatory phase of this test, the aqueous extract of B. trimera at 80 mg/kg (p.o.) significantly decreased the nociceptive behavior, showing more effectiveness when compared to the other tested doses (p < 0.05). Importantly, in the chronic inflammatory model on the 5th day of treatment, the aqueous extract of B. trimera (80 mg/kg p.o.) significantly reduced mechanical allodynia (p < 0.01), heat thermal hyperalgesia (p < 0.001), and paw edema (p < 0.05). There were no changes in the MPO activity, but the data exhibited an equivalent decrease in the number of lymphocytes in the blood of the mice that were treated with B. trimera (80 mg.kg− 1 p.o.) and diclofenac sodium. Conclusion: Taken together, the present data reinforces the potential of the B. trimera aqueous extract as an antiinflammatory and analgesic compound.Antecedentes: As partes aéreas de Baccharis trimera (Less.) são frequentemente utilizadas como chá para tratar várias doenças. Portanto, o objetivo deste estudo foi identificar os constituintes de um extrato aquoso de B. trimera, enfocando suas atividades e propriedades antioxidantes, anti-inflamatórias e antinociceptivas. Para isso, os pesquisadores realizaram ensaios in vivo usando o teste da formalina e o Adjuvante Completo de Freund (FCA) para medir a dor inflamatória aguda e crônica em camundongos. Além disso, a enzima mieloperoxidase (MPO) foi analisada no tecido subcutâneo após a injeção de FCA, juntamente com a contagem de linfócitos no sangue periférico dos camundongos. Resultados: A análise fitoquímica qualitativa indicou a presença de flavonoides e saponinas no extrato aquoso de B. trimera. As análises de cromatografia líquida de alta eficiência (HPLC) mostraram a presença de compostos fenólicos, como ácido clorogênico, ácido elágico, ácido rosmarínico, além de flavonoides, como rutina, quercetina e luteolina. O ensaio DPPH foi utilizado para medir a atividade antioxidante do extrato aquoso de B. trimera e este mostrou um IC50 de 118,18 ± 1,02 μg/mg. Os dados do teste da formalina demonstraram que uma única dose do extrato aquoso de B. trimera não foi capaz de diminuir o comportamento nociceptivo durante a fase neurogênica, em nenhuma das doses testadas (20, 40 ou 80 mg/kg p.o.) . Porém, durante a fase inflamatória desse teste, o extrato aquoso de B. trimera na dose de 80 mg/kg (p.o.) diminuiu significativamente o comportamento nociceptivo, mostrando-se mais eficaz quando comparado às outras doses testadas (p < 0,05). É importante ressaltar que no modelo inflamatório crônico no 5º dia de tratamento, o extrato aquoso de B. trimera (80 mg/kg p.o.) reduziu significativamente a alodinia mecânica (p < 0,01), hiperalgesia térmica térmica (p < 0,001) e edema de pata (p < 0,05). Não houve alteração na atividade da MPO, mas os dados mostraram uma diminuição equivalente no número de linfócitos no sangue dos camundongos tratados com B. trimera (80 mg.kg− 1 p.o.) e diclofenaco sódico. Conclusão: Em conjunto, os presentes dados reforçam o potencial do extrato aquoso de B. trimera como composto antiinflamatório e analgésico.Outra AgênciaengUniversidade Federal de Minas GeraisUFMGBrasilICB - DEPARTAMENTO DE FARMACOLOGIAClinical PhytoscienceAnti-InflamatóriosCapacidade de absorbância de radicais de oxigênioFlavonoidesCompostos fenólicosAnti-inflammatory effectAntioxidant assayFlavonoidsPhenolic compoundsVon Frey testHot plate testBaccharis trimera aqueous extract modulates inflammation and nociception in miceExtrato aquoso de Baccharis trimera modula inflamação e nocicepção em camundongosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://clinphytoscience.springeropen.com/articles/10.1186/s40816-021-00309-wNatiely Silveira de AlmeidaAlexandre de Barros Falcão FerrazCláudia PedronDione Silva CorrêaLuciene Bruno VieiraFlavia Tasmim Techera AntunesAlessandra Hubner de Souzaapplication/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/56259/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALBaccharis trimera aqueous extract modulates inflammation and nociception in mice.pdfBaccharis trimera aqueous extract modulates inflammation and nociception in mice.pdfapplication/pdf481847https://repositorio.ufmg.br/bitstream/1843/56259/2/Baccharis%20trimera%20aqueous%20extract%20modulates%20inflammation%20and%20nociception%20in%20mice.pdf7d3423d7c3868151985dac3e43ceb5daMD521843/562592023-07-14 16:38:10.727oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-07-14T19:38:10Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false |
dc.title.pt_BR.fl_str_mv |
Baccharis trimera aqueous extract modulates inflammation and nociception in mice |
dc.title.alternative.pt_BR.fl_str_mv |
Extrato aquoso de Baccharis trimera modula inflamação e nocicepção em camundongos |
title |
Baccharis trimera aqueous extract modulates inflammation and nociception in mice |
spellingShingle |
Baccharis trimera aqueous extract modulates inflammation and nociception in mice Natiely Silveira de Almeida Anti-inflammatory effect Antioxidant assay Flavonoids Phenolic compounds Von Frey test Hot plate test Anti-Inflamatórios Capacidade de absorbância de radicais de oxigênio Flavonoides Compostos fenólicos |
title_short |
Baccharis trimera aqueous extract modulates inflammation and nociception in mice |
title_full |
Baccharis trimera aqueous extract modulates inflammation and nociception in mice |
title_fullStr |
Baccharis trimera aqueous extract modulates inflammation and nociception in mice |
title_full_unstemmed |
Baccharis trimera aqueous extract modulates inflammation and nociception in mice |
title_sort |
Baccharis trimera aqueous extract modulates inflammation and nociception in mice |
author |
Natiely Silveira de Almeida |
author_facet |
Natiely Silveira de Almeida Alexandre de Barros Falcão Ferraz Cláudia Pedron Dione Silva Corrêa Luciene Bruno Vieira Flavia Tasmim Techera Antunes Alessandra Hubner de Souza |
author_role |
author |
author2 |
Alexandre de Barros Falcão Ferraz Cláudia Pedron Dione Silva Corrêa Luciene Bruno Vieira Flavia Tasmim Techera Antunes Alessandra Hubner de Souza |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Natiely Silveira de Almeida Alexandre de Barros Falcão Ferraz Cláudia Pedron Dione Silva Corrêa Luciene Bruno Vieira Flavia Tasmim Techera Antunes Alessandra Hubner de Souza |
dc.subject.por.fl_str_mv |
Anti-inflammatory effect Antioxidant assay Flavonoids Phenolic compounds Von Frey test Hot plate test |
topic |
Anti-inflammatory effect Antioxidant assay Flavonoids Phenolic compounds Von Frey test Hot plate test Anti-Inflamatórios Capacidade de absorbância de radicais de oxigênio Flavonoides Compostos fenólicos |
dc.subject.other.pt_BR.fl_str_mv |
Anti-Inflamatórios Capacidade de absorbância de radicais de oxigênio Flavonoides Compostos fenólicos |
description |
Background: The aerial parts of Baccharis trimera (Less.) are frequently used as a tea to treat several diseases. Therefore, the aim of this study was to identify the constituents of an aqueous extract of B. trimera, focusing on their antioxidant, anti-inflammation, and antinociception activities and properties. For that, the researchers performed in vivo assays using the formalin test and Freund’s Complete Adjuvant (FCA) to measure the acute and chronic inflammatory pain in mice. Moreover, the myeloperoxidase enzyme (MPO) was analyzed in the subcutaneous tissue after the FCA injection, together with the counting of lymphocytes in the peripheral blood of the mice. Results: The qualitative phytochemical analysis indicated the presence of flavonoids and saponins in the B. trimera aqueous extract. The high-performance liquid chromatography (HPLC) analyses showed the presence of phenolic compounds, such as chlorogenic acid, ellagic acid, rosmarinic acid, as well as flavonoids, such as rutin, quercetin, and luteolin. The DPPH assay was used in order to measure the antioxidant activity of the aqueous extract of B. trimera and this showed an IC50 of 118.18 ± 1.02 μg/mg. The data from the formalin test demonstrated that a single dose of the aqueous extract of B. trimera was not able to decrease the nociceptive behavior during the neurogenic phase, at any of the tested doses (20, 40, or 80 mg/kg p.o.). However, during the inflammatory phase of this test, the aqueous extract of B. trimera at 80 mg/kg (p.o.) significantly decreased the nociceptive behavior, showing more effectiveness when compared to the other tested doses (p < 0.05). Importantly, in the chronic inflammatory model on the 5th day of treatment, the aqueous extract of B. trimera (80 mg/kg p.o.) significantly reduced mechanical allodynia (p < 0.01), heat thermal hyperalgesia (p < 0.001), and paw edema (p < 0.05). There were no changes in the MPO activity, but the data exhibited an equivalent decrease in the number of lymphocytes in the blood of the mice that were treated with B. trimera (80 mg.kg− 1 p.o.) and diclofenac sodium. Conclusion: Taken together, the present data reinforces the potential of the B. trimera aqueous extract as an antiinflammatory and analgesic compound. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021-10-26 |
dc.date.accessioned.fl_str_mv |
2023-07-14T19:38:10Z |
dc.date.available.fl_str_mv |
2023-07-14T19:38:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1843/56259 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1186/s40816-021-00309-w |
dc.identifier.issn.pt_BR.fl_str_mv |
2199-1197 |
dc.identifier.orcid.pt_BR.fl_str_mv |
https://orcid.org/0000-0003-2665-5200 https://orcid.org/0000-0003-4876-8302 https://orcid.org/0000-0002-3496-6749 https://orcid.org/0000-0003-0620-2984 https://orcid.org/0000-0002-6832-2765 |
url |
https://doi.org/10.1186/s40816-021-00309-w http://hdl.handle.net/1843/56259 https://orcid.org/0000-0003-2665-5200 https://orcid.org/0000-0003-4876-8302 https://orcid.org/0000-0002-3496-6749 https://orcid.org/0000-0003-0620-2984 https://orcid.org/0000-0002-6832-2765 |
identifier_str_mv |
2199-1197 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Clinical Phytoscience |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
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Universidade Federal de Minas Gerais |
dc.publisher.initials.fl_str_mv |
UFMG |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
ICB - DEPARTAMENTO DE FARMACOLOGIA |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais |
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