Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus

Detalhes bibliográficos
Autor(a) principal: Matheus Ferreira Inácio
Data de Publicação: 2021
Outros Autores: Franciellen Morais Costa, Otávio Cardoso Filho, Isabela Parolis Martins, Valdo Soares Martins Júnior, Eduardo Robson Duarte, Sônia Ribeiro Arrudas, Yule Roberta Ferreira Nunes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFMG
Texto Completo: https://doi.org/10.33448/rsd-v10i12.20493
http://hdl.handle.net/1843/53799
Resumo: In this study we evaluated the potential of Mauritia flexuosa leaves in the egg hatching and larval development inhibition and for reduction of egg count of this nematode in sheep feces. The leaves of this palm were collected and dehydrated for the production of aqueous and ethanolic extracts with and without tannins. Gas chromatography analysis indicated the presence of fifteen and ten major compounds in the aqueous and ethanolic extracts, respectively, and both showed catechin peaks. The total of condensed tannins for leaves of Mauritia flexuosa was 33.23% ± 2. Aqueous and ethanolic extracts showed 100% anthelmintic activity to inhibit hatchability at 75 mg/ml. The inhibitory concentrations LC90 for aqueous and ethanolic extracts were, respectively, 21.8 and 8.5 mg/ml. The dehydrated leaves powder of M. flexuosa at ≥ 152.08 mg / g of coproculture presented efficiencies greater than 80% for inhibition of larval development. The in vivo administration of aqueous extract at 62.1 mg/kg PC promoted anti-helminthic efficacy of 54.57% and, after 14 days, no clinical signs of toxicity and clinical changes were observed in the treated lambs, indicating potential of this extract for the control alternative of haemonchosis.
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spelling 2023-05-23T12:41:07Z2023-05-23T12:41:07Z2021-09-201012e276101220493https://doi.org/10.33448/rsd-v10i12.204932525-3409http://hdl.handle.net/1843/53799In this study we evaluated the potential of Mauritia flexuosa leaves in the egg hatching and larval development inhibition and for reduction of egg count of this nematode in sheep feces. The leaves of this palm were collected and dehydrated for the production of aqueous and ethanolic extracts with and without tannins. Gas chromatography analysis indicated the presence of fifteen and ten major compounds in the aqueous and ethanolic extracts, respectively, and both showed catechin peaks. The total of condensed tannins for leaves of Mauritia flexuosa was 33.23% ± 2. Aqueous and ethanolic extracts showed 100% anthelmintic activity to inhibit hatchability at 75 mg/ml. The inhibitory concentrations LC90 for aqueous and ethanolic extracts were, respectively, 21.8 and 8.5 mg/ml. The dehydrated leaves powder of M. flexuosa at ≥ 152.08 mg / g of coproculture presented efficiencies greater than 80% for inhibition of larval development. The in vivo administration of aqueous extract at 62.1 mg/kg PC promoted anti-helminthic efficacy of 54.57% and, after 14 days, no clinical signs of toxicity and clinical changes were observed in the treated lambs, indicating potential of this extract for the control alternative of haemonchosis.Neste estudo avaliou-se o potencial das folhas de Mauritia flexuosa na inibição da eclosão e do desenvolvimento larval e redução da contagem de ovos deste nematódeo em fezes de ovinos. As folhas dessa palmeira foram coletadas e desidratadas para a produção dos extratos aquosos e etanólicos com e sem taninos. A análise por cromatografia gasosa indicou a presença de 15 e 10 compostos principais nos extratos aquoso e etanólico, respectivamente, e ambos mostraram picos de catequina. O total de taninos condensados para folhas de Mauritia flexuosa foi de 33,23% ± 2. Os extratos aquoso e etanólico mostraram 100% de atividade anti-helmíntica para inibir a eclodibilidade a 75 mg / ml. As concentrações inibitórias CL90 para os extratos aquosos e etanólicos foram, respectivamente, 21,8 e 8,5 mg / ml. O pó de folhas desidratadas de M. flexuosaa ≥ 152,08 mg / g de coprocultura apresentou eficácias superiores a 80% para inibição do desenvolvimento larval. A administração in vivodo extrato aquoso a 62,1 mg / kg de PC promoveu eficácia anti-helmíntica de 54,57% e, após 14 dias, não foram observados sinais clínicos de toxicidade e alterações clínicas nos cordeiros tratados, indicando potencial deste extrato para o controle alternativo de hemoncose.CNPq - Conselho Nacional de Desenvolvimento Científico e TecnológicoFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas GeraisCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOutra AgênciaengUniversidade Federal de Minas GeraisUFMGBrasilICA - INSTITUTO DE CIÊNCIAS AGRÁRIASICB - DEPARTAMENTO DE MICROBIOLOGIAResearch, Society and DevelopmentCarneirosHaemonchus contortusAnti-helmínticosNematodaBuritiCromatografia a gásLeaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortusFolhas de Mauritia flexuosa L. f. (Arecaceae) tem eficácia, in vitro e in vivo no controle de Haemonchus contortusHojas de Mauritia flexuosa L. f. (Arecaceae) es eficaz in vitro e in vivo en el control de Haemonchus contortusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttps://rsdjournal.org/index.php/rsd/article/view/20493Matheus Ferreira InácioFranciellen Morais CostaOtávio Cardoso FilhoIsabela Parolis MartinsValdo Soares Martins JúniorEduardo Robson DuarteSônia Ribeiro ArrudasYule Roberta Ferreira Nunesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFMGinstname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMGLICENSELicense.txtLicense.txttext/plain; charset=utf-82042https://repositorio.ufmg.br/bitstream/1843/53799/1/License.txtfa505098d172de0bc8864fc1287ffe22MD51ORIGINALLeaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus.pdfLeaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus.pdfapplication/pdf287343https://repositorio.ufmg.br/bitstream/1843/53799/2/Leaves%20of%20Mauritia%20flexuosa%20L.%20f.%20%28Arecaceae%29%20is%20effective%20in%20vitro%20and%20in%20vivo%20in%20the%20control%20of%20Haemonchus%20contortus.pdf3e488e637ef24189a0229b9827e8f97dMD521843/537992023-05-23 17:05:12.083oai:repositorio.ufmg.br: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Repositório de PublicaçõesPUBhttps://repositorio.ufmg.br/oaiopendoar:2023-05-23T20:05:12Repositório Institucional da UFMG - Universidade Federal de Minas Gerais (UFMG)false
dc.title.pt_BR.fl_str_mv Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus
dc.title.alternative.pt_BR.fl_str_mv Folhas de Mauritia flexuosa L. f. (Arecaceae) tem eficácia, in vitro e in vivo no controle de Haemonchus contortus
Hojas de Mauritia flexuosa L. f. (Arecaceae) es eficaz in vitro e in vivo en el control de Haemonchus contortus
title Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus
spellingShingle Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus
Matheus Ferreira Inácio
Carneiros
Haemonchus contortus
Anti-helmínticos
Nematoda
Buriti
Cromatografia a gás
title_short Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus
title_full Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus
title_fullStr Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus
title_full_unstemmed Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus
title_sort Leaves of Mauritia flexuosa L. f. (Arecaceae) is effective in vitro and in vivo in the control of Haemonchus contortus
author Matheus Ferreira Inácio
author_facet Matheus Ferreira Inácio
Franciellen Morais Costa
Otávio Cardoso Filho
Isabela Parolis Martins
Valdo Soares Martins Júnior
Eduardo Robson Duarte
Sônia Ribeiro Arrudas
Yule Roberta Ferreira Nunes
author_role author
author2 Franciellen Morais Costa
Otávio Cardoso Filho
Isabela Parolis Martins
Valdo Soares Martins Júnior
Eduardo Robson Duarte
Sônia Ribeiro Arrudas
Yule Roberta Ferreira Nunes
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Matheus Ferreira Inácio
Franciellen Morais Costa
Otávio Cardoso Filho
Isabela Parolis Martins
Valdo Soares Martins Júnior
Eduardo Robson Duarte
Sônia Ribeiro Arrudas
Yule Roberta Ferreira Nunes
dc.subject.other.pt_BR.fl_str_mv Carneiros
Haemonchus contortus
Anti-helmínticos
Nematoda
Buriti
Cromatografia a gás
topic Carneiros
Haemonchus contortus
Anti-helmínticos
Nematoda
Buriti
Cromatografia a gás
description In this study we evaluated the potential of Mauritia flexuosa leaves in the egg hatching and larval development inhibition and for reduction of egg count of this nematode in sheep feces. The leaves of this palm were collected and dehydrated for the production of aqueous and ethanolic extracts with and without tannins. Gas chromatography analysis indicated the presence of fifteen and ten major compounds in the aqueous and ethanolic extracts, respectively, and both showed catechin peaks. The total of condensed tannins for leaves of Mauritia flexuosa was 33.23% ± 2. Aqueous and ethanolic extracts showed 100% anthelmintic activity to inhibit hatchability at 75 mg/ml. The inhibitory concentrations LC90 for aqueous and ethanolic extracts were, respectively, 21.8 and 8.5 mg/ml. The dehydrated leaves powder of M. flexuosa at ≥ 152.08 mg / g of coproculture presented efficiencies greater than 80% for inhibition of larval development. The in vivo administration of aqueous extract at 62.1 mg/kg PC promoted anti-helminthic efficacy of 54.57% and, after 14 days, no clinical signs of toxicity and clinical changes were observed in the treated lambs, indicating potential of this extract for the control alternative of haemonchosis.
publishDate 2021
dc.date.issued.fl_str_mv 2021-09-20
dc.date.accessioned.fl_str_mv 2023-05-23T12:41:07Z
dc.date.available.fl_str_mv 2023-05-23T12:41:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1843/53799
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.33448/rsd-v10i12.20493
dc.identifier.issn.pt_BR.fl_str_mv 2525-3409
url https://doi.org/10.33448/rsd-v10i12.20493
http://hdl.handle.net/1843/53799
identifier_str_mv 2525-3409
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Research, Society and Development
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.publisher.initials.fl_str_mv UFMG
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
ICB - DEPARTAMENTO DE MICROBIOLOGIA
publisher.none.fl_str_mv Universidade Federal de Minas Gerais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFMG
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
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institution UFMG
reponame_str Repositório Institucional da UFMG
collection Repositório Institucional da UFMG
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